BuphA.00118.a
Glucose-1-phosphate thymidylyltransferase
| CENTER ID: | BuphA.00118.a |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00118.a.B1.GE40197 | Full length( BuphA.00118.a ) | 1 | 297 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00118.a.B1.PW38413 | Full length( BuphA.00118.a ) | 1 | 297 |
Structures
6N0U
DEPOSITED: 11/7/2018
DETERMINATION: XRay
CLONE: BuphA.00118.a.B1.GE40197
PROTEIN: BuphA.00118.a.B1.PW38413
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131815 |
| PATRIC ID: | fig|391038.7.peg.2465 |
| RefSeq: | YP_001858539.1 |
| UniProt: | B2JFC5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARKGIILAG GSGTRLYPIT HVVSKQLLPV YDKPMIYYPL STLMVAGIRD VLIISTPQDT PRFEAMLGDG SQWGMNIQYA VQPSPDGLAQ AFIIGREFVG NDPSALILGD NIFYGHDLAK QLERANARTD GATVFAYHVH DPERYGVVEF DKDFRALSIE EKPAKPRSNY AVTGLYFYDN QVCDIAADIK PSARGELEIT DVNSRYLSQK TLDVEIMGRG YAWLDTGTHD SLIEAATFIA TLQKRQGLVV ACPEEIAYRR KWINAEQLLA LARPLSKNAY GQYLQNLLTD QVAWPSV
NT Sequence
ATGGCGCGCA AGGGCATTAT TCTGGCGGGC GGCTCGGGCA CCCGGCTATA TCCTATAACG CATGTCGTGT CGAAGCAGTT GCTTCCCGTG TACGACAAGC CGATGATTTA TTACCCGCTG TCGACGCTGA TGGTCGCGGG CATCCGCGAT GTCCTGATCA TCTCGACGCC GCAAGACACG CCGCGCTTTG AAGCGATGCT CGGCGACGGC AGCCAGTGGG GGATGAACAT CCAGTACGCG GTGCAGCCGT CGCCGGATGG GCTTGCGCAG GCATTCATCA TTGGTCGCGA GTTTGTTGGC AATGATCCGT CGGCGCTGAT TCTCGGTGAC AACATTTTCT ACGGCCACGA TCTGGCGAAG CAGCTGGAAC GAGCGAATGC GCGCACGGAT GGCGCAACGG TTTTCGCGTA TCACGTGCAT GATCCCGAGC GCTATGGCGT GGTCGAGTTC GACAAGGACT TCCGCGCGCT GTCGATCGAA GAAAAGCCCG CGAAGCCGCG CTCGAACTAC GCAGTGACGG GCCTGTACTT CTACGACAAT CAAGTTTGCG ATATTGCTGC CGATATCAAG CCTTCGGCTC GCGGTGAACT CGAAATCACG GACGTGAATT CGCGTTACCT GTCGCAGAAG ACGCTTGATG TCGAGATCAT GGGCCGCGGC TATGCGTGGC TCGATACGGG TACGCACGAT TCACTGATCG AAGCGGCGAC GTTCATCGCG ACGCTGCAAA AGCGGCAGGG GCTTGTGGTT GCATGTCCGG AAGAAATTGC GTATCGGCGC AAGTGGATCA ATGCCGAGCA ATTGCTTGCG CTCGCCAGGC CGCTGTCGAA GAACGCTTAC GGGCAGTATC TGCAAAACCT TCTTACGGAC CAAGTCGCAT GGCCATCAGT G
Details for BuphA.00118.a.B1.GE40197
| HARVESTED ON: | 5/9/2016 |
| SEQUENCED ON: | 5/23/2016 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA RKGIILAGGS GTRLYPITHV VSKQLLPVYD KPMIYYPLST LMVAGIRDVL IISTPQDTPR FEAMLGDGSQ WGMNIQYAVQ PSPDGLAQAF IIGREFVGND PSALILGDNI FYGHDLAKQL ERANARTDGA TVFAYHVHDP ERYGVVEFDK DFRALSIEEK PAKPRSNYAV TGLYFYDNQV CDIAADIKPS ARGELEITDV NSRYLSQKTL DVEIMGRGYA WLDTGTHDSL IEAATFIATL QKRQGLVVAC PEEIAYRRKW INAEQLLALA RPLSKNAYGQ YLQNLLXXXV AWPSV
Validated NT Sequence
ttactcactt acactgatgg ccatgcgacn nnnnnnnnna gaaggttttg cagatactgc ccgtaagcgt tcttcgacag cggcctggcg agcgcaagca attgctcggc attgatccac ttgcgccgat acgcaatttc ttccggacat gcaaccacaa gcccctgccg cttttgcagc gtcgcgatga acgtcgccgc ttcgatcagt gaatcgtgcg tacccgtatc gagccacgca tagccgcggc ccatgatctc gacatcaagc gtcttctgcg acaggtaacg cgaattcacg tccgtgattt cgagttcacc gcgagccgaa ggcttgatat cggcagcaat atcgcaaact tgattgtcgt agaagtacag gcccgtcact gcgtagttcg agcgcggctt cgcgggcttt tcttcgatcg acagcgcgcg gaagtccttg tcgaactcga ccacgccata gcgctcggga tcatgcacgt gatacgcgaa aaccgttgcg ccatccgtgc gcgcattcgc tcgttccagc tgcttcgcca gatcgtggcc gtagaaaatg ttgtcaccga gaatcagcgc cgacggatca ttgccaacaa actcgcgacc aatgatgaat gcctgcgcaa gcccatccgg cgacggctgc accgcgtact ggatgttcat cccccactgg ctgccgtcgc cgagcatcgc ttcaaagcgc ggcgtgtctt gcggcgtcga gatgatcagg acatcgcgga tgcccgcgac catcagcgtc gacagcgggt aataaatcat cggcttgtcg tacacgggaa gcaactgctt cgacacgaca tgcgttatag gatatagccg ggtgcccgag ccgcccgcca gaataatgcc cttgcgcgcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMA RKGIILAGGS GTRLYPITHV VSKQLLPVYD KPMIYYPLST LMVAGIRDVL IISTPQDTPR FEAMLGDGSQ WGMNIQYAVQ PSPDGLAQAF IIGREFVGND PSALILGDNI FYGHDLAKQL ERANARTDGA TVFAYHVHDP ERYGVVEFDK DFRALSIEEK PAKPRSNYAV TGLYFYDNQV CDIAADIKPS ARGELEITDV NSRYLSQKTL DVEIMGRGYA WLDTGTHDSL IEAATFIATL QKRQGLVVAC PEEIAYRRKW INAEQLLALA RPLSKNAYGQ YLQNLLTDQV AWPSV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc gcaagggcat tattctggcg ggcggctcgg gcacccggct atatcctata acgcatgtcg tgtcgaagca gttgcttccc gtgtacgaca agccgatgat ttattacccg ctgtcgacgc tgatggtcgc gggcatccgc gatgtcctga tcatctcgac gccgcaagac acgccgcgct ttgaagcgat gctcggcgac ggcagccagt gggggatgaa catccagtac gcggtgcagc cgtcgccgga tgggcttgcg caggcattca tcattggtcg cgagtttgtt ggcaatgatc cgtcggcgct gattctcggt gacaacattt tctacggcca cgatctggcg aagcagctgg aacgagcgaa tgcgcgcacg gatggcgcaa cggttttcgc gtatcacgtg catgatcccg agcgctatgg cgtggtcgag ttcgacaagg acttccgcgc gctgtcgatc gaagaaaagc ccgcgaagcc gcgctcgaac tacgcagtga cgggcctgta cttctacgac aatcaagttt gcgatattgc tgccgatatc aagccttcgg ctcgcggtga actcgaaatc acggacgtga attcgcgtta cctgtcgcag aagacgcttg atgtcgagat catgggccgc ggctatgcgt ggctcgatac gggtacgcac gattcactga tcgaagcggc gacgttcatc gcgacgctgc aaaagcggca ggggcttgtg gttgcatgtc cggaagaaat tgcgtatcgg cgcaagtgga tcaatgccga gcaattgctt gcgctcgcca ggccgctgtc gaagaacgct tacgggcagt atctgcaaaa ccttcttacg gaccaagtcg catggccatc agtgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuphA.00118.a.B1.PW38413
| PURIFICATION DATe: | 1/16/2018 |
| CONCENTRATION: | 31.12mg/ml |
| OBSERVED MW: | 31kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA RKGIILAGGS GTRLYPITHV VSKQLLPVYD KPMIYYPLST LMVAGIRDVL IISTPQDTPR FEAMLGDGSQ WGMNIQYAVQ PSPDGLAQAF IIGREFVGND PSALILGDNI FYGHDLAKQL ERANARTDGA TVFAYHVHDP ERYGVVEFDK DFRALSIEEK PAKPRSNYAV TGLYFYDNQV CDIAADIKPS ARGELEITDV NSRYLSQKTL DVEIMGRGYA WLDTGTHDSL IEAATFIATL QKRQGLVVAC PEEIAYRRKW INAEQLLALA RPLSKNAYGQ YLQNLLXXXV AWPSV
Validated NT Sequence
ttactcactt acactgatgg ccatgcgacn nnnnnnnnna gaaggttttg cagatactgc ccgtaagcgt tcttcgacag cggcctggcg agcgcaagca attgctcggc attgatccac ttgcgccgat acgcaatttc ttccggacat gcaaccacaa gcccctgccg cttttgcagc gtcgcgatga acgtcgccgc ttcgatcagt gaatcgtgcg tacccgtatc gagccacgca tagccgcggc ccatgatctc gacatcaagc gtcttctgcg acaggtaacg cgaattcacg tccgtgattt cgagttcacc gcgagccgaa ggcttgatat cggcagcaat atcgcaaact tgattgtcgt agaagtacag gcccgtcact gcgtagttcg agcgcggctt cgcgggcttt tcttcgatcg acagcgcgcg gaagtccttg tcgaactcga ccacgccata gcgctcggga tcatgcacgt gatacgcgaa aaccgttgcg ccatccgtgc gcgcattcgc tcgttccagc tgcttcgcca gatcgtggcc gtagaaaatg ttgtcaccga gaatcagcgc cgacggatca ttgccaacaa actcgcgacc aatgatgaat gcctgcgcaa gcccatccgg cgacggctgc accgcgtact ggatgttcat cccccactgg ctgccgtcgc cgagcatcgc ttcaaagcgc ggcgtgtctt gcggcgtcga gatgatcagg acatcgcgga tgcccgcgac catcagcgtc gacagcgggt aataaatcat cggcttgtcg tacacgggaa gcaactgctt cgacacgaca tgcgttatag gatatagccg ggtgcccgag ccgcccgcca gaataatgcc cttgcgcgcc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMA RKGIILAGGS GTRLYPITHV VSKQLLPVYD KPMIYYPLST LMVAGIRDVL IISTPQDTPR FEAMLGDGSQ WGMNIQYAVQ PSPDGLAQAF IIGREFVGND PSALILGDNI FYGHDLAKQL ERANARTDGA TVFAYHVHDP ERYGVVEFDK DFRALSIEEK PAKPRSNYAV TGLYFYDNQV CDIAADIKPS ARGELEITDV NSRYLSQKTL DVEIMGRGYA WLDTGTHDSL IEAATFIATL QKRQGLVVAC PEEIAYRRKW INAEQLLALA RPLSKNAYGQ YLQNLLTDQV AWPSV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGC GCAAGGGCAT TATTCTGGCG GGCGGCTCGG GCACCCGGCT ATATCCTATA ACGCATGTCG TGTCGAAGCA GTTGCTTCCC GTGTACGACA AGCCGATGAT TTATTACCCG CTGTCGACGC TGATGGTCGC GGGCATCCGC GATGTCCTGA TCATCTCGAC GCCGCAAGAC ACGCCGCGCT TTGAAGCGAT GCTCGGCGAC GGCAGCCAGT GGGGGATGAA CATCCAGTAC GCGGTGCAGC CGTCGCCGGA TGGGCTTGCG CAGGCATTCA TCATTGGTCG CGAGTTTGTT GGCAATGATC CGTCGGCGCT GATTCTCGGT GACAACATTT TCTACGGCCA CGATCTGGCG AAGCAGCTGG AACGAGCGAA TGCGCGCACG GATGGCGCAA CGGTTTTCGC GTATCACGTG CATGATCCCG AGCGCTATGG CGTGGTCGAG TTCGACAAGG ACTTCCGCGC GCTGTCGATC GAAGAAAAGC CCGCGAAGCC GCGCTCGAAC TACGCAGTGA CGGGCCTGTA CTTCTACGAC AATCAAGTTT GCGATATTGC TGCCGATATC AAGCCTTCGG CTCGCGGTGA ACTCGAAATC ACGGACGTGA ATTCGCGTTA CCTGTCGCAG AAGACGCTTG ATGTCGAGAT CATGGGCCGC GGCTATGCGT GGCTCGATAC GGGTACGCAC GATTCACTGA TCGAAGCGGC GACGTTCATC GCGACGCTGC AAAAGCGGCA GGGGCTTGTG GTTGCATGTC CGGAAGAAAT TGCGTATCGG CGCAAGTGGA TCAATGCCGA GCAATTGCTT GCGCTCGCCA GGCCGCTGTC GAAGAACGCT TACGGGCAGT ATCTGCAAAA CCTTCTTACG GACCAAGTCG CATGGCCATC AGTGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT