BuphA.00117.a

Intradiol ring-cleavage dioxygenase

CENTER ID: BuphA.00117.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00117.a.B1.GE40196 Full length( BuphA.00117.a ) 1 293
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00117.a.B1.PS38289 Full length( BuphA.00117.a ) 1 293

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.5920
RefSeq: YP_001861744.1
UniProt: B2JUH6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTDEREAER ALTEQVVASF ANAEDARLRT LMQSLVRHMH AFVREVEPTE AEWMAAIRFL TATGKMCDDL VRQEFILLSD TLGVSMLVDA INHRLATGAT DTTVFGPFYI EGMPERAYGE NMAFTPGTPA LVHGRVLTTL GEPVAGALLD VWQTADNGMY SGQDTAQPHG NLRGRYRTDA EGRFAITTIV PVSYPIPTDG PVGQMLRATG RHPWRPAHLH FMIKAPGYRT LVTHLFDKDD PYLQSDAVFG VKPSLLVAYG ERSAGDELAR RFGFSGDYRE ARYDFVVDES ATP
NT Sequence
ATGACGACGG ACGAACGAGA GGCCGAACGA GCCTTGACCG AACAGGTGGT GGCGAGTTTT GCGAACGCGG AAGATGCACG GCTGCGCACC CTGATGCAAA GCCTCGTGCG GCACATGCAT GCGTTCGTGC GTGAAGTCGA GCCGACGGAA GCCGAGTGGA TGGCGGCGAT CCGCTTCCTG ACCGCGACGG GCAAGATGTG CGACGACCTG GTGCGGCAGG AGTTCATCCT GTTGTCGGAT ACGCTGGGCG TGTCGATGCT GGTCGATGCG ATCAATCATC GGCTGGCCAC CGGTGCGACC GATACGACGG TGTTCGGCCC GTTCTATATC GAAGGAATGC CGGAGCGTGC TTACGGCGAG AACATGGCCT TCACGCCGGG CACACCCGCG CTCGTGCATG GCCGTGTGCT GACGACGTTG GGCGAACCCG TCGCGGGGGC GTTGCTCGAC GTATGGCAGA CGGCCGACAA CGGCATGTAT TCCGGGCAGG ACACGGCGCA GCCGCATGGC AATCTGCGCG GACGCTATCG CACGGACGCT GAAGGCCGGT TTGCGATCAC GACAATCGTG CCCGTCAGCT ATCCGATCCC GACGGACGGC CCGGTTGGAC AGATGCTCCG AGCGACAGGC AGGCATCCGT GGCGTCCCGC GCATCTGCAT TTCATGATCA AGGCGCCGGG CTATCGCACG CTCGTCACAC ATCTGTTCGA CAAGGACGAT CCGTATCTGC AATCGGATGC TGTGTTCGGC GTGAAGCCTT CGCTGCTGGT CGCGTACGGC GAGCGCTCCG CAGGCGACGA ACTCGCGCGC CGCTTCGGCT TCAGCGGCGA TTATCGCGAA GCGCGCTACG ACTTCGTTGT CGATGAGAGC GCGACGCCA
Details for BuphA.00117.a.B1.GE40196
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 33kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT TDEREAERAL TEQVVASFAN AEDARLRTLM QSLVRHMHAF VREVEPTEAE WMAAIRFLTA TGKMCDDLVR QEFILLSDTL GVSMLVDAIN HRLATGATDT TVFGPFYIEG MPERAYGENM AFTPGTPALV HGRVLTTLGE PVAGALLDVW QTADNGMYSG QDTAQPHGNL RGRYRTDAEG RFAITTIVPV SYPIPTDGPV GQMLRATGRH PWRPAHLHFM IKAPGYRTLV THLFDKDDPY LQSDAVFGVK PSLLVAYGER SAGDELARRF GFSGDYREAR YDXXXDESAT P
Validated NT Sequence
ttactcactt atggcgtcgc gctctcatcn nnnnnnnngt cgtagcgcgc ttcgcgataa tcgccgctga agccgaagcg gcgcgcgagt tcgtcgcctg cggagcgctc gccgtacgcg accagcagcg aaggcttcac gccgaacaca gcatccgatt gcagatacgg atcgtccttg tcgaacagat gtgtgacgag cgtgcgatag cccggcgcct tgatcatgaa atgcagatgc gcgggacgcc acggatgcct gcctgtcgct cggagcatct gtccaaccgg gccgtccgtc gggatcggat agctgacggg cacgattgtc gtgatcgcaa accggccttc agcgtccgtg cgatagcgtc cgcgcagatt gccatgcggc tgcgccgtgt cctgcccgga atacatgccg ttgtcggccg tctgccatac gtcgagcaac gcccccgcga cgggttcgcc caacgtcgtc agcacacggc catgcacgag cgcgggtgtg cccggcgtga aggccatgtt ctcgccgtaa gcacgctccg gcattccttc gatatagaac gggccgaaca ccgtcgtatc ggtcgcaccg gtggccagcc gatgattgat cgcatcgacc agcatcgaca cgcccagcgt atccgacaac aggatgaact cctgccgcac caggtcgtcg cacatcttgc ccgtcgcggt caggaagcgg atcgccgcca tccactcggc ttccgtcggc tcgacttcac gcacgaacgc atgcatgtgc cgcacgaggc tttgcatcag ggtgcgcagc cgtgcatctt ccgcgttcgc aaaactcgcc accacctgtt cggtcaaggc tcgttcggcc tctcgttcgt ccgtcgtcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMT TDEREAERAL TEQVVASFAN AEDARLRTLM QSLVRHMHAF VREVEPTEAE WMAAIRFLTA TGKMCDDLVR QEFILLSDTL GVSMLVDAIN HRLATGATDT TVFGPFYIEG MPERAYGENM AFTPGTPALV HGRVLTTLGE PVAGALLDVW QTADNGMYSG QDTAQPHGNL RGRYRTDAEG RFAITTIVPV SYPIPTDGPV GQMLRATGRH PWRPAHLHFM IKAPGYRTLV THLFDKDDPY LQSDAVFGVK PSLLVAYGER SAGDELARRF GFSGDYREAR YDFVVDESAT P
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cggacgaacg agaggccgaa cgagccttga ccgaacaggt ggtggcgagt tttgcgaacg cggaagatgc acggctgcgc accctgatgc aaagcctcgt gcggcacatg catgcgttcg tgcgtgaagt cgagccgacg gaagccgagt ggatggcggc gatccgcttc ctgaccgcga cgggcaagat gtgcgacgac ctggtgcggc aggagttcat cctgttgtcg gatacgctgg gcgtgtcgat gctggtcgat gcgatcaatc atcggctggc caccggtgcg accgatacga cggtgttcgg cccgttctat atcgaaggaa tgccggagcg tgcttacggc gagaacatgg ccttcacgcc gggcacaccc gcgctcgtgc atggccgtgt gctgacgacg ttgggcgaac ccgtcgcggg ggcgttgctc gacgtatggc agacggccga caacggcatg tattccgggc aggacacggc gcagccgcat ggcaatctgc gcggacgcta tcgcacggac gctgaaggcc ggtttgcgat cacgacaatc gtgcccgtca gctatccgat cccgacggac ggcccggttg gacagatgct ccgagcgaca ggcaggcatc cgtggcgtcc cgcgcatctg catttcatga tcaaggcgcc gggctatcgc acgctcgtca cacatctgtt cgacaaggac gatccgtatc tgcaatcgga tgctgtgttc ggcgtgaagc cttcgctgct ggtcgcgtac ggcgagcgct ccgcaggcga cgaactcgcg cgccgcttcg gcttcagcgg cgattatcgc gaagcgcgct acgacttcgt tgtcgatgag agcgcgacgc catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuphA.00117.a.B1.PS38289
PURIFICATION DATe: 5/23/2017
CONCENTRATION: 17.2mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Bufer: 20mM HEPES, 300mM NaCl, 5% Glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT TDEREAERAL TEQVVASFAN AEDARLRTLM QSLVRHMHAF VREVEPTEAE WMAAIRFLTA TGKMCDDLVR QEFILLSDTL GVSMLVDAIN HRLATGATDT TVFGPFYIEG MPERAYGENM AFTPGTPALV HGRVLTTLGE PVAGALLDVW QTADNGMYSG QDTAQPHGNL RGRYRTDAEG RFAITTIVPV SYPIPTDGPV GQMLRATGRH PWRPAHLHFM IKAPGYRTLV THLFDKDDPY LQSDAVFGVK PSLLVAYGER SAGDELARRF GFSGDYREAR YDXXXDESAT P
Validated NT Sequence
ttactcactt atggcgtcgc gctctcatcn nnnnnnnngt cgtagcgcgc ttcgcgataa tcgccgctga agccgaagcg gcgcgcgagt tcgtcgcctg cggagcgctc gccgtacgcg accagcagcg aaggcttcac gccgaacaca gcatccgatt gcagatacgg atcgtccttg tcgaacagat gtgtgacgag cgtgcgatag cccggcgcct tgatcatgaa atgcagatgc gcgggacgcc acggatgcct gcctgtcgct cggagcatct gtccaaccgg gccgtccgtc gggatcggat agctgacggg cacgattgtc gtgatcgcaa accggccttc agcgtccgtg cgatagcgtc cgcgcagatt gccatgcggc tgcgccgtgt cctgcccgga atacatgccg ttgtcggccg tctgccatac gtcgagcaac gcccccgcga cgggttcgcc caacgtcgtc agcacacggc catgcacgag cgcgggtgtg cccggcgtga aggccatgtt ctcgccgtaa gcacgctccg gcattccttc gatatagaac gggccgaaca ccgtcgtatc ggtcgcaccg gtggccagcc gatgattgat cgcatcgacc agcatcgaca cgcccagcgt atccgacaac aggatgaact cctgccgcac caggtcgtcg cacatcttgc ccgtcgcggt caggaagcgg atcgccgcca tccactcggc ttccgtcggc tcgacttcac gcacgaacgc atgcatgtgc cgcacgaggc tttgcatcag ggtgcgcagc cgtgcatctt ccgcgttcgc aaaactcgcc accacctgtt cggtcaaggc tcgttcggcc tctcgttcgt ccgtcgtcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMT TDEREAERAL TEQVVASFAN AEDARLRTLM QSLVRHMHAF VREVEPTEAE WMAAIRFLTA TGKMCDDLVR QEFILLSDTL GVSMLVDAIN HRLATGATDT TVFGPFYIEG MPERAYGENM AFTPGTPALV HGRVLTTLGE PVAGALLDVW QTADNGMYSG QDTAQPHGNL RGRYRTDAEG RFAITTIVPV SYPIPTDGPV GQMLRATGRH PWRPAHLHFM IKAPGYRTLV THLFDKDDPY LQSDAVFGVK PSLLVAYGER SAGDELARRF GFSGDYREAR YDFVVDESAT P
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA CGGACGAACG AGAGGCCGAA CGAGCCTTGA CCGAACAGGT GGTGGCGAGT TTTGCGAACG CGGAAGATGC ACGGCTGCGC ACCCTGATGC AAAGCCTCGT GCGGCACATG CATGCGTTCG TGCGTGAAGT CGAGCCGACG GAAGCCGAGT GGATGGCGGC GATCCGCTTC CTGACCGCGA CGGGCAAGAT GTGCGACGAC CTGGTGCGGC AGGAGTTCAT CCTGTTGTCG GATACGCTGG GCGTGTCGAT GCTGGTCGAT GCGATCAATC ATCGGCTGGC CACCGGTGCG ACCGATACGA CGGTGTTCGG CCCGTTCTAT ATCGAAGGAA TGCCGGAGCG TGCTTACGGC GAGAACATGG CCTTCACGCC GGGCACACCC GCGCTCGTGC ATGGCCGTGT GCTGACGACG TTGGGCGAAC CCGTCGCGGG GGCGTTGCTC GACGTATGGC AGACGGCCGA CAACGGCATG TATTCCGGGC AGGACACGGC GCAGCCGCAT GGCAATCTGC GCGGACGCTA TCGCACGGAC GCTGAAGGCC GGTTTGCGAT CACGACAATC GTGCCCGTCA GCTATCCGAT CCCGACGGAC GGCCCGGTTG GACAGATGCT CCGAGCGACA GGCAGGCATC CGTGGCGTCC CGCGCATCTG CATTTCATGA TCAAGGCGCC GGGCTATCGC ACGCTCGTCA CACATCTGTT CGACAAGGAC GATCCGTATC TGCAATCGGA TGCTGTGTTC GGCGTGAAGC CTTCGCTGCT GGTCGCGTAC GGCGAGCGCT CCGCAGGCGA CGAACTCGCG CGCCGCTTCG GCTTCAGCGG CGATTATCGC GAAGCGCGCT ACGACTTCGT TGTCGATGAG AGCGCGACGC CATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT