BuphA.00116.b
4-hydroxythreonine-4-phosphate dehydrogenase
| CENTER ID: | BuphA.00116.b |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00116.b.B1.GE35964 | Full length( BuphA.00116.b ) | 1 | 342 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00116.b.B1.PS01665 | Full length( BuphA.00116.b ) | 1 | 342 |
Structures
4JQP
DEPOSITED: 3/20/2013
DETERMINATION: XRay
CLONE: BuphA.00116.b.B1.GE35964
PROTEIN: BuphA.00116.b.B1.PS01665
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_135377 |
| PATRIC ID: | fig|391038.7.peg.6148 |
| RefSeq: | YP_001861948.1 |
| UniProt: | B2JVC5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSTYSRDQI MSNYLPVIGI TMGDAAGVGA EVVVKSLAHA SVYAQCRPLV IGDAKRLERA NQIVGGEMKI RRIEDASEAR YEQGTIDCID LGLIPDDLPF GQLSAIAGDA AYQYIKRAVE LAQSGKIDAI CTAPLNKEAL HAGGHKYPGH TEMLAHLTGV DEVSMMLVAP QLRVIHVTTH IGIIDAIRKI EPGLVQRTIE RGNATLVKAG IERPRIGVCG INPHAGENGL FGYGEEEEKI IPAVTLLQER GLDVTGPLPA DTLFFRAGRG DFDLVVAMYH DQGHGPVKVL GLEAGVNVTV GLEVIRTSVD HGTAFDIAGK GVVDEGSMLE ALRQGAELAT RR
NT Sequence
ATGAACAGCA CGTATTCACG AGATCAAATC ATGAGCAACT ACCTTCCCGT AATAGGCATC ACGATGGGCG ATGCAGCCGG CGTCGGCGCT GAAGTCGTCG TCAAGAGCCT CGCGCATGCA TCCGTCTATG CGCAATGCCG TCCGCTCGTG ATCGGCGACG CGAAGCGCCT CGAACGCGCA AATCAGATCG TGGGCGGCGA GATGAAAATT CGCCGCATCG AAGACGCCTC CGAAGCGCGC TACGAGCAGG GCACGATCGA CTGCATCGAC CTCGGCCTGA TTCCCGACGA CCTGCCGTTC GGCCAGCTGT CGGCTATCGC GGGCGATGCC GCGTACCAGT ACATCAAGCG CGCCGTGGAA CTCGCGCAGT CGGGCAAGAT CGACGCGATC TGCACGGCGC CTCTGAACAA GGAAGCGCTG CATGCGGGCG GTCACAAGTA TCCGGGCCAC ACGGAAATGC TGGCGCATCT GACGGGTGTC GACGAAGTGT CGATGATGCT CGTCGCCCCG CAACTGCGCG TGATCCATGT GACGACGCAC ATCGGCATCA TCGACGCCAT CCGCAAGATC GAGCCGGGCC TCGTGCAGCG CACGATCGAA CGCGGCAATG CGACGCTCGT GAAGGCGGGG ATCGAGCGTC CGCGCATCGG CGTGTGCGGG ATCAATCCGC ATGCGGGCGA GAACGGTCTG TTCGGCTACG GCGAAGAGGA AGAGAAGATC ATCCCCGCGG TGACGCTGCT GCAGGAGCGC GGTCTCGACG TGACGGGCCC GTTGCCCGCC GACACCCTGT TTTTCCGCGC GGGCCGCGGC GATTTCGATC TGGTGGTGGC GATGTATCAC GACCAGGGGC ATGGTCCCGT GAAGGTGCTC GGTCTCGAAG CGGGCGTGAA CGTGACGGTG GGGCTGGAAG TGATCCGCAC GTCCGTCGAT CACGGCACCG CTTTCGACAT CGCGGGCAAG GGTGTCGTCG ATGAAGGCAG CATGCTCGAA GCGCTGCGCC AGGGCGCCGA ACTGGCGACG CGCCGC
Details for BuphA.00116.b.B1.GE35964
| HARVESTED ON: | 8/28/2012 |
| SEQUENCED ON: | 8/31/2012 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MSNYLPVIGI TMGDAAGVGA EVVVKSLAHA SVYAQCRPLV IGDAKRLERA NQIVGGEMKI RRIEDASEAR YEQGTIDCID LGLIPDDLPF GQLSAIAGDA AYQYIKRAVE LAQSGKIDAI CTAPLNKEAL HAGGHKYPGH TEMLAHLTGV DEVSMMLVAP QLRVIHVTTH IGIIDAIRKI EPGLVQRTIE RGNATLVKAG IERPRIGVCG INPHAGENGL FGYGEEEEKI IPAVTLLQER GLDVTGPLPA DTLFFRAGRG DFDLVVAMYH DQGHGPVKVL GLEAGVNVTV GLEVIRTSVD HGTAFDIAGK GVVDEGSMLE ALRQGAELAT R
Validated NT Sequence
ggcgcgtcgc cagttcggcg ccctggcgca gcgcttcgag catgctgcct tcatcgacga cacccttgcc cgcgatgtcg aaagcggtgc cgtgatcgac ggacgtgcgg atcacttcca gccccaccgt cacgttcacg cccgcttcga gaccgagcac cttcacggga ccatgcccct ggtcgtgata catcgccacc accagatcga aatcgccgcg gcccgcgcgg aaaaacaggg tgtcggcggg caacgggccc gtcacgtcga gaccgcgctc ctgcagcagc gtcaccgcgg ggatgatctt ctcttcctct tcgccgtagc cgaacagacc gttctcgccc gcatgcggat tgatcccgca cacgccgatg cgcggacgct cgatccccgc cttcacgagc gtcgcattgc cgcgttcgat cgtgcgctgc acgaggcccg gctcgatctt gcggatggcg tcgatgatgc cgatgtgcgt cgtcacatgg atcacgcgca gttgcggggc gacgagcatc atcgacactt cgtcgacacc cgtcagatgc gccagcattt ccgtgtggcc cggatacttg tgaccgcccg catgcagcgc ttccttgttc agaggcgccg tgcagatcgc gtcgatcttg cccgactgcg cgagttccac ggcgcgcttg atgtactggt acgcggcatc gcccgcgata gccgacagct ggccgaacgg caggtcgtcg ggaatcaggc cgaggtcgat gcagtcgatc gtgccctgct cgtagcgcgc ttcggaggcg tcttcgatgc ggcgaatttt catctcgccg cccacgatct gatttgcgcg ttcgaggcgc ttcgcgtcgc cgatcacgag cggacggcat tgcgcataga cggatgcatg cgcgaggctc ttgacgacga cttcagcgcc gacgccggct gcatcgccca tcgtgatgcc tattacggga aggtagttgc tcatgatttg atctcgtgaa tacgtgct
Expected Protein Sequence
MAHHHHHHMN STYSRDQIMS NYLPVIGITM GDAAGVGAEV VVKSLAHASV YAQCRPLVIG DAKRLERANQ IVGGEMKIRR IEDASEARYE QGTIDCIDLG LIPDDLPFGQ LSAIAGDAAY QYIKRAVELA QSGKIDAICT APLNKEALHA GGHKYPGHTE MLAHLTGVDE VSMMLVAPQL RVIHVTTHIG IIDAIRKIEP GLVQRTIERG NATLVKAGIE RPRIGVCGIN PHAGENGLFG YGEEEEKIIP AVTLLQERGL DVTGPLPADT LFFRAGRGDF DLVVAMYHDQ GHGPVKVLGL EAGVNVTVGL EVIRTSVDHG TAFDIAGKGV VDEGSMLEAL RQGAELATRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca gcacgtattc acgagatcaa atcatgagca actaccttcc cgtaataggc atcacgatgg gcgatgcagc cggcgtcggc gctgaagtcg tcgtcaagag cctcgcgcat gcatccgtct atgcgcaatg ccgtccgctc gtgatcggcg acgcgaagcg cctcgaacgc gcaaatcaga tcgtgggcgg cgagatgaaa attcgccgca tcgaagacgc ctccgaagcg cgctacgagc agggcacgat cgactgcatc gacctcggcc tgattcccga cgacctgccg ttcggccagc tgtcggctat cgcgggcgat gccgcgtacc agtacatcaa gcgcgccgtg gaactcgcgc agtcgggcaa gatcgacgcg atctgcacgg cgcctctgaa caaggaagcg ctgcatgcgg gcggtcacaa gtatccgggc cacacggaaa tgctggcgca tctgacgggt gtcgacgaag tgtcgatgat gctcgtcgcc ccgcaactgc gcgtgatcca tgtgacgacg cacatcggca tcatcgacgc catccgcaag atcgagccgg gcctcgtgca gcgcacgatc gaacgcggca atgcgacgct cgtgaaggcg gggatcgagc gtccgcgcat cggcgtgtgc gggatcaatc cgcatgcggg cgagaacggt ctgttcggct acggcgaaga ggaagagaag atcatccccg cggtgacgct gctgcaggag cgcggtctcg acgtgacggg cccgttgccc gccgacaccc tgtttttccg cgcgggccgc ggcgatttcg atctggtggt ggcgatgtat cacgaccagg ggcatggtcc cgtgaaggtg ctcggtctcg aagcgggcgt gaacgtgacg gtggggctgg aagtgatccg cacgtccgtc gatcacggca ccgctttcga catcgcgggc aagggtgtcg tcgatgaagg cagcatgctc gaagcgctgc gccagggcgc cgaactggcg acgcgccgct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BuphA.00116.b.B1.PS01665
| PURIFICATION DATe: | 11/1/2012 |
| CONCENTRATION: | 17mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSNYLPVIGI TMGDAAGVGA EVVVKSLAHA SVYAQCRPLV IGDAKRLERA NQIVGGEMKI RRIEDASEAR YEQGTIDCID LGLIPDDLPF GQLSAIAGDA AYQYIKRAVE LAQSGKIDAI CTAPLNKEAL HAGGHKYPGH TEMLAHLTGV DEVSMMLVAP QLRVIHVTTH IGIIDAIRKI EPGLVQRTIE RGNATLVKAG IERPRIGVCG INPHAGENGL FGYGEEEEKI IPAVTLLQER GLDVTGPLPA DTLFFRAGRG DFDLVVAMYH DQGHGPVKVL GLEAGVNVTV GLEVIRTSVD HGTAFDIAGK GVVDEGSMLE ALRQGAELAT R
Validated NT Sequence
ggcgcgtcgc cagttcggcg ccctggcgca gcgcttcgag catgctgcct tcatcgacga cacccttgcc cgcgatgtcg aaagcggtgc cgtgatcgac ggacgtgcgg atcacttcca gccccaccgt cacgttcacg cccgcttcga gaccgagcac cttcacggga ccatgcccct ggtcgtgata catcgccacc accagatcga aatcgccgcg gcccgcgcgg aaaaacaggg tgtcggcggg caacgggccc gtcacgtcga gaccgcgctc ctgcagcagc gtcaccgcgg ggatgatctt ctcttcctct tcgccgtagc cgaacagacc gttctcgccc gcatgcggat tgatcccgca cacgccgatg cgcggacgct cgatccccgc cttcacgagc gtcgcattgc cgcgttcgat cgtgcgctgc acgaggcccg gctcgatctt gcggatggcg tcgatgatgc cgatgtgcgt cgtcacatgg atcacgcgca gttgcggggc gacgagcatc atcgacactt cgtcgacacc cgtcagatgc gccagcattt ccgtgtggcc cggatacttg tgaccgcccg catgcagcgc ttccttgttc agaggcgccg tgcagatcgc gtcgatcttg cccgactgcg cgagttccac ggcgcgcttg atgtactggt acgcggcatc gcccgcgata gccgacagct ggccgaacgg caggtcgtcg ggaatcaggc cgaggtcgat gcagtcgatc gtgccctgct cgtagcgcgc ttcggaggcg tcttcgatgc ggcgaatttt catctcgccg cccacgatct gatttgcgcg ttcgaggcgc ttcgcgtcgc cgatcacgag cggacggcat tgcgcataga cggatgcatg cgcgaggctc ttgacgacga cttcagcgcc gacgccggct gcatcgccca tcgtgatgcc tattacggga aggtagttgc tcatgatttg atctcgtgaa tacgtgct
Expressed Protein Sequence
MAHHHHHHMN STYSRDQIMS NYLPVIGITM GDAAGVGAEV VVKSLAHASV YAQCRPLVIG DAKRLERANQ IVGGEMKIRR IEDASEARYE QGTIDCIDLG LIPDDLPFGQ LSAIAGDAAY QYIKRAVELA QSGKIDAICT APLNKEALHA GGHKYPGHTE MLAHLTGVDE VSMMLVAPQL RVIHVTTHIG IIDAIRKIEP GLVQRTIERG NATLVKAGIE RPRIGVCGIN PHAGENGLFG YGEEEEKIIP AVTLLQERGL DVTGPLPADT LFFRAGRGDF DLVVAMYHDQ GHGPVKVLGL EAGVNVTVGL EVIRTSVDHG TAFDIAGKGV VDEGSMLEAL RQGAELATRR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACA GCACGTATTC ACGAGATCAA ATCATGAGCA ACTACCTTCC CGTAATAGGC ATCACGATGG GCGATGCAGC CGGCGTCGGC GCTGAAGTCG TCGTCAAGAG CCTCGCGCAT GCATCCGTCT ATGCGCAATG CCGTCCGCTC GTGATCGGCG ACGCGAAGCG CCTCGAACGC GCAAATCAGA TCGTGGGCGG CGAGATGAAA ATTCGCCGCA TCGAAGACGC CTCCGAAGCG CGCTACGAGC AGGGCACGAT CGACTGCATC GACCTCGGCC TGATTCCCGA CGACCTGCCG TTCGGCCAGC TGTCGGCTAT CGCGGGCGAT GCCGCGTACC AGTACATCAA GCGCGCCGTG GAACTCGCGC AGTCGGGCAA GATCGACGCG ATCTGCACGG CGCCTCTGAA CAAGGAAGCG CTGCATGCGG GCGGTCACAA GTATCCGGGC CACACGGAAA TGCTGGCGCA TCTGACGGGT GTCGACGAAG TGTCGATGAT GCTCGTCGCC CCGCAACTGC GCGTGATCCA TGTGACGACG CACATCGGCA TCATCGACGC CATCCGCAAG ATCGAGCCGG GCCTCGTGCA GCGCACGATC GAACGCGGCA ATGCGACGCT CGTGAAGGCG GGGATCGAGC GTCCGCGCAT CGGCGTGTGC GGGATCAATC CGCATGCGGG CGAGAACGGT CTGTTCGGCT ACGGCGAAGA GGAAGAGAAG ATCATCCCCG CGGTGACGCT GCTGCAGGAG CGCGGTCTCG ACGTGACGGG CCCGTTGCCC GCCGACACCC TGTTTTTCCG CGCGGGCCGC GGCGATTTCG ATCTGGTGGT GGCGATGTAT CACGACCAGG GGCATGGTCC CGTGAAGGTG CTCGGTCTCG AAGCGGGCGT GAACGTGACG GTGGGGCTGG AAGTGATCCG CACGTCCGTC GATCACGGCA CCGCTTTCGA CATCGCGGGC AAGGGTGTCG TCGATGAAGG CAGCATGCTC GAAGCGCTGC GCCAGGGCGC CGAACTGGCG ACGCGCCGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT