BuphA.00116.a

4-hydroxythreonine-4-phosphate dehydrogenase

CENTER ID: BuphA.00116.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00116.a.B1.GE35963 Full length( BuphA.00116.a ) 1 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.413
RefSeq: YP_001856637.1
UniProt: B2JCX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIAAQPSNDP LHIAITTGEP AGVGPELTAQ ALAAAGAHWP DARFSVLGDS ALMDERAKAV GVDWAAQQGR RIAMQHHALA VPALAGKLDA QNGPYVLGLL DAAIDGALAG TFDAIVTAPL QKSTINDAGV PFTGHTEYLA ERTGTPHVVM MLAGTGARPL RVALATTHLP LKDVSAALTV DGIADTLRII DHDLRRHFGL PAPRILVTGL NPHAGENGYL GREEIDVISP ALRLANGQGI DARGPYPADT LFQPRYLNEA DCVLAMFHDQ GLPVLKYATF GEGINVTLGL PIIRTSVDHG TALDLAGTGR ADAGSLIAAI DTAVFMARNR RAA
NT Sequence
ATGATCGCTG CCCAGCCCTC GAACGACCCG CTTCACATCG CGATCACCAC GGGCGAGCCG GCTGGCGTCG GTCCTGAACT GACGGCACAG GCGCTCGCAG CGGCGGGCGC ACATTGGCCG GACGCGCGCT TCTCGGTGCT CGGCGACAGC GCTCTGATGG ACGAGCGTGC GAAGGCCGTC GGCGTCGACT GGGCCGCGCA GCAGGGCCGT CGCATTGCCA TGCAGCATCA TGCGCTTGCC GTGCCGGCGC TGGCCGGGAA GCTGGATGCA CAGAACGGCC CGTATGTGCT CGGGCTGCTC GATGCGGCTA TCGATGGCGC GCTGGCGGGT ACGTTCGACG CTATCGTCAC CGCGCCGCTG CAGAAGAGCA CGATCAACGA TGCCGGCGTG CCGTTCACCG GCCACACCGA ATATCTGGCG GAGCGCACGG GGACGCCGCA TGTCGTGATG ATGCTGGCGG GCACAGGCGC AAGGCCGCTG CGCGTCGCGC TGGCGACTAC GCATCTGCCG CTGAAGGACG TGTCGGCGGC GTTGACGGTC GACGGCATCG CCGACACGCT GCGCATCATC GATCACGACT TGCGTCGCCA TTTTGGCCTG CCCGCGCCGC GCATTCTCGT CACGGGTCTG AATCCTCACG CGGGCGAGAA CGGCTATCTC GGGCGCGAGG AAATCGATGT GATCTCGCCC GCGCTGCGAC TGGCGAACGG GCAGGGCATC GACGCGCGCG GTCCGTATCC GGCGGATACT CTGTTCCAGC CGCGTTATCT GAACGAAGCC GATTGCGTGC TCGCGATGTT CCACGATCAG GGCCTGCCCG TGCTCAAATA TGCGACGTTC GGCGAAGGCA TCAACGTGAC CTTGGGCCTG CCGATCATCC GCACGTCCGT CGACCACGGC ACGGCGCTCG ATCTTGCCGG TACGGGCCGC GCGGATGCGG GCAGCCTGAT CGCCGCGATC GACACCGCTG TTTTCATGGC GCGCAACCGG CGCGCCGCG
Details for BuphA.00116.a.B1.GE35963
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMI AAQPSNDPLH IAITTGEPAG VGPELTAQAL AAAGAHWPDA RFSVLGDSAL MDERAKAVGV DWAAQQGRRI AMQHHALAVP ALAGKLDAQN GPYVLGLLDA AIDGALAGTF DAIVTAPLQK STINDAGVPF TGHTEYLAER TGTPHVVMML AGTGARPLRV ALATTHLPLK DVSAALTVDG IADTLRIIDH DLRRHFGLPA PRILVTGLNP HAGENGYLGR EEIDVISPAL RLANGXXSTR AVRIXXILXS XAXLXEAXXX XXXXIXXXXX XXXXXXXX
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgatc gctgcccagc cctcgaacga cccgcttcac atcgcgatca ccacgggcga gccggctggc gtcggtcctg aactgacggc acaggcgctc gcagcggcgg gcgcacattg gccggacgcg cgcttctcgg tgctcggcga cagcgctctg atggacgagc gtgcgaaggc cgtcggcgtc gactgggccg cgcagcaggg ccgtcgcatt gccatgcagc atcatgcgct tgccgtgccg gcgctggccg ggaagctgga tgcacagaac ggcccgtatg tgctcgggct gctcgatgcg gctatcgatg gcgcgctggc gggtacgttc gacgctatcg tcaccgcgcc gctgcagaag agcacgatca acgatgccgg cgtgccgttc accggccaca ccgaatatct ggcggagcgc acggggacgc cgcatgtcgt gatgatgctg gcgggcacag gcgcaaggcc gctgcgcgtc gcgctggcga ctacgcatct gccgctgaag gacgtgtcgg cggcgttgac ggtcgacggc atcgccgaca cgctgcgcat catcgatcac gacttgcgtc gccattttgg cctgcccgcg ccgcgcattc tcgtcacggg tctgaatcct cacgcgggcg agaacggcta tctcgggcgc gaggaaatcg atgtgatctc gcccgcgctg cgactggcga acgggnnnnn atcgacgcgc gcggtccgta tccnnnngat actctgntcc ancgcntntc tgnacgaagc cnangnnnnt cnnnatnnnn ngatcngnnn nncnnngntn annntgcnnc ntgnnannnt nanntgactn gnnnncnann ancnnnnncn nnnnnnnnnn nnnnnnnnnn nnngnngnnn cngnnnnncn ntnnnnncnn ttnnnngnnn nnnngnnnnn nnnnnnnnnn na
Expected Protein Sequence
MAHHHHHHMI AAQPSNDPLH IAITTGEPAG VGPELTAQAL AAAGAHWPDA RFSVLGDSAL MDERAKAVGV DWAAQQGRRI AMQHHALAVP ALAGKLDAQN GPYVLGLLDA AIDGALAGTF DAIVTAPLQK STINDAGVPF TGHTEYLAER TGTPHVVMML AGTGARPLRV ALATTHLPLK DVSAALTVDG IADTLRIIDH DLRRHFGLPA PRILVTGLNP HAGENGYLGR EEIDVISPAL RLANGQGIDA RGPYPADTLF QPRYLNEADC VLAMFHDQGL PVLKYATFGE GINVTLGLPI IRTSVDHGTA LDLAGTGRAD AGSLIAAIDT AVFMARNRRA A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg ctgcccagcc ctcgaacgac ccgcttcaca tcgcgatcac cacgggcgag ccggctggcg tcggtcctga actgacggca caggcgctcg cagcggcggg cgcacattgg ccggacgcgc gcttctcggt gctcggcgac agcgctctga tggacgagcg tgcgaaggcc gtcggcgtcg actgggccgc gcagcagggc cgtcgcattg ccatgcagca tcatgcgctt gccgtgccgg cgctggccgg gaagctggat gcacagaacg gcccgtatgt gctcgggctg ctcgatgcgg ctatcgatgg cgcgctggcg ggtacgttcg acgctatcgt caccgcgccg ctgcagaaga gcacgatcaa cgatgccggc gtgccgttca ccggccacac cgaatatctg gcggagcgca cggggacgcc gcatgtcgtg atgatgctgg cgggcacagg cgcaaggccg ctgcgcgtcg cgctggcgac tacgcatctg ccgctgaagg acgtgtcggc ggcgttgacg gtcgacggca tcgccgacac gctgcgcatc atcgatcacg acttgcgtcg ccattttggc ctgcccgcgc cgcgcattct cgtcacgggt ctgaatcctc acgcgggcga gaacggctat ctcgggcgcg aggaaatcga tgtgatctcg cccgcgctgc gactggcgaa cgggcagggc atcgacgcgc gcggtccgta tccggcggat actctgttcc agccgcgtta tctgaacgaa gccgattgcg tgctcgcgat gttccacgat cagggcctgc ccgtgctcaa atatgcgacg ttcggcgaag gcatcaacgt gaccttgggc ctgccgatca tccgcacgtc cgtcgaccac ggcacggcgc tcgatcttgc cggtacgggc cgcgcggatg cgggcagcct gatcgccgcg atcgacaccg ctgttttcat ggcgcgcaac cggcgcgccg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat