BuphA.00113.c

3-oxoacyl-(Acyl-carrier-protein) synthase 2

CENTER ID: BuphA.00113.c
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00113.c.A1.GE32279 Full length( BuphA.00113.c ) 1 428
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.876
RefSeq: YP_001857067.1
UniProt: B2JFJ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSCGFCFVI YGKGVTVSRR RVVVTGLGLI SPVGNNVADG WANLVAGKSG IANITKFDAS NFSTRFAGEV KGFNIEDYIA GKEARHMDTF IHYGVAAGIQ AMQDSGLEIT DANSERVGVV VGSGIGGLPM IEVTQTELLN RGPRRISPFF VPASIINMIS GHLSIKFGLK GPNLSMVTAC TTGLHCIGEA SRLIEYGDAD VMIAGGAEST VSPLGIGGFA AARALSQRND DPATASRPWD KDRDGFVLGE GAGVMVLEEY EHAKARGAKI YAEVGGYGMS GDAYHMTAPL EDGDGARRCM IAAMKNAGVN PDSVNYLNAH GTSTPLGDLA ETTGIKRAFG DHSKKIVVNS TKSMTGHLLG GAGGLESVFT VLAVHHQVSP PTINIFNQDP ECDLDYCANT AREMKIDVAL KNSFGFGGTN GTLVFKRA
NT Sequence
GTGCGCTCCT GTGGCTTTTG TTTTGTCATC TATGGAAAAG GGGTTACCGT GAGCCGTCGT CGTGTTGTTG TTACAGGCCT GGGGCTTATT TCGCCTGTTG GCAATAATGT TGCCGACGGC TGGGCCAATC TGGTCGCCGG CAAGTCTGGC ATCGCCAACA TCACGAAGTT CGACGCGTCG AACTTCTCCA CACGTTTCGC CGGTGAGGTG AAGGGCTTCA ACATTGAAGA CTACATCGCC GGCAAGGAAG CGCGCCATAT GGATACGTTC ATCCATTACG GCGTGGCTGC CGGCATCCAG GCGATGCAGG ACAGCGGCCT CGAAATCACC GATGCGAACT CGGAACGCGT TGGCGTCGTG GTCGGTTCGG GCATCGGCGG CCTGCCAATG ATCGAAGTCA CGCAGACGGA ACTGCTGAAT CGCGGCCCGC GCCGGATTTC GCCGTTCTTC GTGCCGGCGT CGATCATCAA CATGATCTCC GGTCATCTGT CGATCAAGTT CGGCCTGAAG GGTCCGAACC TGTCGATGGT GACGGCTTGT ACGACGGGTC TGCATTGCAT CGGCGAGGCA TCGCGTCTGA TCGAGTACGG CGACGCCGAC GTGATGATCG CGGGCGGCGC GGAATCGACC GTTTCGCCCC TCGGCATCGG CGGCTTCGCG GCGGCGCGCG CGCTGTCGCA GCGTAACGAC GATCCGGCGA CGGCGAGCCG TCCTTGGGAC AAGGACCGCG ACGGTTTCGT GCTCGGCGAG GGTGCGGGCG TGATGGTGCT CGAAGAGTAC GAACATGCGA AGGCGCGCGG CGCGAAGATC TACGCGGAAG TCGGCGGTTA CGGCATGAGC GGCGACGCGT ATCACATGAC GGCCCCGCTC GAAGACGGCG ACGGCGCACG CCGCTGCATG ATCGCAGCGA TGAAGAACGC TGGCGTCAAC CCCGATTCGG TGAATTACCT GAACGCGCAC GGCACCTCGA CGCCGCTCGG CGACCTCGCC GAAACCACGG GCATCAAGCG CGCATTCGGC GATCACTCGA AGAAGATCGT CGTAAATTCG ACAAAGTCGA TGACGGGTCA CCTGCTGGGC GGTGCGGGCG GTCTGGAGTC CGTGTTCACG GTCCTGGCTG TGCATCACCA GGTGTCGCCG CCGACCATCA ACATCTTCAA CCAGGATCCG GAATGCGATC TCGACTACTG CGCCAACACG GCGCGGGAGA TGAAGATCGA CGTCGCGCTG AAGAACTCGT TCGGGTTCGG CGGCACGAAC GGCACGCTGG TTTTCAAGCG CGCC
Details for BuphA.00113.c.A1.GE32279
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 67
PERCENT COVERAGE: 90
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRSCGFCF VIYGKGVTVS RRRVVVTGLG LISPVGNNVA DGWANLVAGK SGIANITKFD ASNFSTRFAG EVKGFNIEDY IAGKEARHMD TFIHYGVAAG IQAMQDSGLE ITDANSERVG VVVGSGIGGL PMIEVTQTEL LNRGPRRISP FFVPASIINM ISGHLSIKFG LKGPNLSMVT ACTTGLHCIG EASRLIEYGD ADVMIAGGAE STVSPLGIGG FAAARALSQR NDDPATASRP WGQGPRRFRA RRGCGRDGAR RVRTCEGARR EDLRGSXRLR HERRRVSHDG PARRXRXRTX LHDRSDXNAG XTPXXXXTXT XXAXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttgtttac tttaagnagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgcg ctcctgtggc ttttgttttg tcatctatgg aaaaggggtt accgtgagcc gtcgtcgtgt tgttgttaca ggcctggggc ttatttcgcc tgttggcaat aatgttgccg acggctgggc caatctggtc gccggcaagt ctggcatcgc caacatcacg aagttcgacg cgtcgaactt ctccacacgt ttcgccggtg aggtgaaggg cttcaacatt gaagactaca tcgccggcaa ggaagcgcgc catatggata cgttcatcca ttacggcgtg gctgccggca tccaggcgat gcaggacagc ggcctcgaaa tcaccgatgc gaactcggaa cgcgttggcg tcgtggtcgg ttcgggcatc ggcggcctgc caatgatcga agtcacgcag acggaactgc tgaatcgcgg cccgcgccgg atttcgccgt tcttcgtgcc ggcgtcgatc atcaacatga tctccggtca tctgtcgatc aagttcggcc tgaagggtcc gaacctgtcg atggtgacgg cttgtacgac gggtctgcat tgcatcggcg aggcatcgcg tctgatcgag tacggcgacg ccgacgtgat gatcgcgggc ggcgcggaat cgaccgtttc gcccctcggc atcggcggct tcgcggcggc gcgcgcgctg tcgcagcgta acgacgatcc ggcgacggcg agccgtcctt ggggacaagg accgcgacgg tttcgtgctc ggcgagggtg cgggcgtgat ggtgctcgaa gagtacgaac atgcgaaggc gcgcggcgcg aagatctacg cggaagtcng cggttacggc atgagcggcg acgcgtatca catgacggcc ccgctcgaag anggcgangn cgcacgncgc tgcatgatcg cagcgatnan aacgctggnn tnaccccgan ncngngannt acctgnacgn gcanggcncn cgncgncnct nggnnacntc nncnaaannn cnggnntnnn nnnnnnantn ggnnatnann nnnnnaaann nnnnnanntn nnnaaannnn nnnnnnnngg nnnncnnnnn nnnnnnnnnn nnnntnnnnn nnnnnnnnnn nnnnnnngnn nnnnnnnnnn gnnngnnnnn ncnnnnnnnn nnnnntnnnn a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRSCGFCF VIYGKGVTVS RRRVVVTGLG LISPVGNNVA DGWANLVAGK SGIANITKFD ASNFSTRFAG EVKGFNIEDY IAGKEARHMD TFIHYGVAAG IQAMQDSGLE ITDANSERVG VVVGSGIGGL PMIEVTQTEL LNRGPRRISP FFVPASIINM ISGHLSIKFG LKGPNLSMVT ACTTGLHCIG EASRLIEYGD ADVMIAGGAE STVSPLGIGG FAAARALSQR NDDPATASRP WDKDRDGFVL GEGAGVMVLE EYEHAKARGA KIYAEVGGYG MSGDAYHMTA PLEDGDGARR CMIAAMKNAG VNPDSVNYLN AHGTSTPLGD LAETTGIKRA FGDHSKKIVV NSTKSMTGHL LGGAGGLESV FTVLAVHHQV SPPTINIFNQ DPECDLDYCA NTAREMKIDV ALKNSFGFGG TNGTLVFKRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgctcc tgtggctttt gttttgtcat ctatggaaaa ggggttaccg tgagccgtcg tcgtgttgtt gttacaggcc tggggcttat ttcgcctgtt ggcaataatg ttgccgacgg ctgggccaat ctggtcgccg gcaagtctgg catcgccaac atcacgaagt tcgacgcgtc gaacttctcc acacgtttcg ccggtgaggt gaagggcttc aacattgaag actacatcgc cggcaaggaa gcgcgccata tggatacgtt catccattac ggcgtggctg ccggcatcca ggcgatgcag gacagcggcc tcgaaatcac cgatgcgaac tcggaacgcg ttggcgtcgt ggtcggttcg ggcatcggcg gcctgccaat gatcgaagtc acgcagacgg aactgctgaa tcgcggcccg cgccggattt cgccgttctt cgtgccggcg tcgatcatca acatgatctc cggtcatctg tcgatcaagt tcggcctgaa gggtccgaac ctgtcgatgg tgacggcttg tacgacgggt ctgcattgca tcggcgaggc atcgcgtctg atcgagtacg gcgacgccga cgtgatgatc gcgggcggcg cggaatcgac cgtttcgccc ctcggcatcg gcggcttcgc ggcggcgcgc gcgctgtcgc agcgtaacga cgatccggcg acggcgagcc gtccttggga caaggaccgc gacggtttcg tgctcggcga gggtgcgggc gtgatggtgc tcgaagagta cgaacatgcg aaggcgcgcg gcgcgaagat ctacgcggaa gtcggcggtt acggcatgag cggcgacgcg tatcacatga cggccccgct cgaagacggc gacggcgcac gccgctgcat gatcgcagcg atgaagaacg ctggcgtcaa ccccgattcg gtgaattacc tgaacgcgca cggcacctcg acgccgctcg gcgacctcgc cgaaaccacg ggcatcaagc gcgcattcgg cgatcactcg aagaagatcg tcgtaaattc gacaaagtcg atgacgggtc acctgctggg cggtgcgggc ggtctggagt ccgtgttcac ggtcctggct gtgcatcacc aggtgtcgcc gccgaccatc aacatcttca accaggatcc ggaatgcgat ctcgactact gcgccaacac ggcgcgggag atgaagatcg acgtcgcgct gaagaactcg ttcgggttcg gcggcacgaa cggcacgctg gttttcaagc gcgcctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa