BuphA.00113.b

Beta-ketoacyl synthase

CENTER ID: BuphA.00113.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00113.b.A1.GE32184 Full length( BuphA.00113.b ) 1 408
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.744
RefSeq: YP_001856939.1
UniProt: B2JEW4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRVQGMQRV VVTGMGIVSC IGNTLDDVSA ALREGRSGIT HVDAWRERGF GTQVAGVASV DGEPPFDRKL ERFMGDTARF ACHAARKAID DAGLDIAALR SHHVGAVLGS GVGTMSAYDA AMAVANTRGV EKTPPYTVPQ AMSSTASANV AQVFGIEGVT YSPSSACTTS ALAIGQAMQL IQTGRQRIVL AGGSECLHDN MTLMFDAMHA LSRRFNDTPA SASRPYDTAR DGFVIAAGGG VLVLEALDHA LARGARIYAE LTGFGHCTDG AGMVSPHAPG IARAMQVALD EAGARPDYVN AHAPSTPLGD IEELRALQTV FGLDVPPFSS TKGLTGHPLG ACGAHEAIYT LLMMRDGFIA GTVGIEQIEP LAQQLPLVQA TREARIDRAL SVSFGFGGSC ASLMFARI
NT Sequence
ATGACGCGTG TGCAAGGCAT GCAGCGCGTC GTCGTCACGG GCATGGGCAT CGTGTCGTGC ATCGGCAATA CGCTCGACGA CGTCAGCGCT GCGCTGCGTG AAGGACGCAG CGGCATCACG CATGTCGATG CGTGGCGCGA GCGCGGCTTC GGCACCCAGG TCGCGGGTGT CGCGTCGGTG GACGGCGAGC CGCCATTCGA TCGCAAGCTT GAACGATTCA TGGGCGATAC CGCGCGCTTC GCGTGCCACG CGGCGCGCAA GGCCATCGAC GACGCAGGTC TCGATATCGC CGCGCTGCGT TCGCATCACG TCGGTGCCGT GCTCGGCTCG GGTGTCGGCA CGATGTCGGC GTATGACGCG GCGATGGCCG TCGCGAACAC GCGCGGTGTC GAGAAAACGC CGCCGTACAC GGTGCCGCAG GCGATGAGCA GCACCGCCTC CGCGAACGTC GCGCAGGTGT TCGGCATCGA GGGCGTCACG TATTCGCCGT CGTCGGCGTG CACGACGTCG GCGCTCGCGA TCGGCCAGGC CATGCAGCTG ATTCAAACGG GACGTCAACG GATCGTTCTC GCAGGTGGCA GCGAATGCCT GCACGACAAC ATGACGCTGA TGTTCGACGC GATGCATGCG CTGTCGCGCC GCTTCAACGA CACGCCCGCC AGCGCCTCGC GACCTTACGA TACGGCGCGC GACGGCTTCG TGATCGCAGC GGGCGGCGGC GTGCTCGTGC TCGAAGCGCT CGATCATGCG CTCGCACGCG GCGCGCGCAT CTATGCGGAG CTGACGGGGT TCGGCCACTG CACGGACGGC GCGGGCATGG TGTCGCCGCA CGCGCCGGGC ATCGCGCGCG CAATGCAGGT TGCGCTCGAC GAAGCGGGCG CGCGTCCCGA TTACGTGAAT GCGCACGCGC CGTCGACGCC GCTCGGCGAC ATCGAGGAAC TGCGCGCGCT GCAAACGGTG TTCGGTTTGG ACGTGCCGCC GTTTTCGTCG ACGAAGGGGC TGACGGGGCA TCCCCTCGGC GCATGCGGGG CGCATGAGGC GATCTACACG CTGCTCATGA TGCGCGACGG TTTTATCGCA GGGACGGTGG GCATCGAACA GATCGAGCCG CTCGCACAAC AACTGCCGCT GGTGCAGGCC ACGCGCGAAG CGAGGATCGA TCGCGCGTTG TCGGTGTCGT TCGGTTTCGG GGGGAGTTGC GCGAGCCTGA TGTTTGCCAG GATT
Details for BuphA.00113.b.A1.GE32184
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MTRVQGMQRV VVTGMGIVSC IGNTLDDVXA ALREGRSGIT HVDAWRERGF XTQVAGVASV DGEPPFDRKL ERFXXDTARF ACHAARKAID DAGLDIAALR SHHVGAVLGS GVGTMSAYDA AMAVANTRGV EKTPPYTVPQ AMSSTASANV AQVFGIEGVT YSPSSACTTS ALAIGQAMQL IQTGRQRIVL AGGSECLHDN MTLMFDAMHA LSRRFNDTPA SASRPYDTAR DGFVIAAGGG VLVLEALDHA LARGARIYAE LTGFGHCTDG AGMVSPHAPG IARAMQVALD EAGARPDYVN AHAPSTPLGD IEELRALQTV FGLDVPPFSS TKGLTGHPLG ACGAHEAIYT LLMMRDGFIA GTVGIEQIEP LAQQLPLVQA TREARIDRAL SVSFGFGGSC ASLMFARI
Validated NT Sequence
ttaaatcctg gcaaacatca ggctcgcgca actccccccg aaaccgaacg acaccgacaa cgcgcgatcg atcctcgctt cgcgcgtggc ctgcaccagc ggcagttgtt gtgcgagcgg ctcgatctgt tcgatgccca ccgtccctgc gataaaaccg tcgcgcatca tgagcagcgt gtagatcgcc tcatgcgccc cgcatgcgcc gaggggatgc cccgtcagcc ccttcgtcga cgaaaacggc ggcacgtcca aaccgaacac cgtttgcagc gcgcgcagtt cctcgatgtc gccgagcggc gtcgacggcg cgtgcgcatt cacgtaatcg ggacgcgcgc ccgcttcgtc gagcgcaacc tgcattgcgc gcgcgatgcc cggcgcgtgc ggcgacacca tgcccgcgcc gtccgtgcag tggccgaacc ccgtcagctc cgcatagatg cgcgcgccgc gtgcgagcgc atgatcgagc gcttcgagca cgagcacgcc gccgcccgct gcgatcacga agccgtcgcg cgccgtatcg taaggtcgcg aggcgctggc gggcgtgtcg ttgaagcggc gcgacagcgc atgcatcgcg tcgaacatca gcgtcatgtt gtcgtgcagg cattcgctgc cacctgcgag aacgatccgt tgacgtcccg tttgaatcag ctgcatggcc tggccgatcg cgagcgccga cgtcgtgcac gcngacgacg gcgaatacgt gacgccctcg atgccgaaca cctgcgcgac gttcgcggag gcggtgctgc tcatcgcctg cggcaccgtg tacggcggcg ttttctcgac accgcgcgtg ttcgcgacgg ccatcgccgc gtcatacgcc gacatcgtgc cgacacccga gccgagcacg gcaccgacgt gatgcgaacg cagcgcggcg atatcgagac ctgcgtcgtc gatggccttg cgcgccgcgt ggcacgcgaa gcgcgcggta tcnnccanga atcgttcaag cttgcgatcg aatggcggct cgccgtccac cgacgcgaca cccgcgacct gggtgcngaa gccgcgctcg cgccacgcat cgacatgcgt gatgccgctg cgtccttcac gcagcgcagc gcngacgtcg tcgagcgtat tgccgatgca cgacacgatg cccatgcccg tgacgacgac gcgctgcatg ccttgcacac gcgtcatcga accaggacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVQGMQR VVVTGMGIVS CIGNTLDDVS AALREGRSGI THVDAWRERG FGTQVAGVAS VDGEPPFDRK LERFMGDTAR FACHAARKAI DDAGLDIAAL RSHHVGAVLG SGVGTMSAYD AAMAVANTRG VEKTPPYTVP QAMSSTASAN VAQVFGIEGV TYSPSSACTT SALAIGQAMQ LIQTGRQRIV LAGGSECLHD NMTLMFDAMH ALSRRFNDTP ASASRPYDTA RDGFVIAAGG GVLVLEALDH ALARGARIYA ELTGFGHCTD GAGMVSPHAP GIARAMQVAL DEAGARPDYV NAHAPSTPLG DIEELRALQT VFGLDVPPFS STKGLTGHPL GACGAHEAIY TLLMMRDGFI AGTVGIEQIE PLAQQLPLVQ ATREARIDRA LSVSFGFGGS CASLMFARI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcgtgtg caaggcatgc agcgcgtcgt cgtcacgggc atgggcatcg tgtcgtgcat cggcaatacg ctcgacgacg tcagcgctgc gctgcgtgaa ggacgcagcg gcatcacgca tgtcgatgcg tggcgcgagc gcggcttcgg cacccaggtc gcgggtgtcg cgtcggtgga cggcgagccg ccattcgatc gcaagcttga acgattcatg ggcgataccg cgcgcttcgc gtgccacgcg gcgcgcaagg ccatcgacga cgcaggtctc gatatcgccg cgctgcgttc gcatcacgtc ggtgccgtgc tcggctcggg tgtcggcacg atgtcggcgt atgacgcggc gatggccgtc gcgaacacgc gcggtgtcga gaaaacgccg ccgtacacgg tgccgcaggc gatgagcagc accgcctccg cgaacgtcgc gcaggtgttc ggcatcgagg gcgtcacgta ttcgccgtcg tcggcgtgca cgacgtcggc gctcgcgatc ggccaggcca tgcagctgat tcaaacggga cgtcaacgga tcgttctcgc aggtggcagc gaatgcctgc acgacaacat gacgctgatg ttcgacgcga tgcatgcgct gtcgcgccgc ttcaacgaca cgcccgccag cgcctcgcga ccttacgata cggcgcgcga cggcttcgtg atcgcagcgg gcggcggcgt gctcgtgctc gaagcgctcg atcatgcgct cgcacgcggc gcgcgcatct atgcggagct gacggggttc ggccactgca cggacggcgc gggcatggtg tcgccgcacg cgccgggcat cgcgcgcgca atgcaggttg cgctcgacga agcgggcgcg cgtcccgatt acgtgaatgc gcacgcgccg tcgacgccgc tcggcgacat cgaggaactg cgcgcgctgc aaacggtgtt cggtttggac gtgccgccgt tttcgtcgac gaaggggctg acggggcatc ccctcggcgc atgcggggcg catgaggcga tctacacgct gctcatgatg cgcgacggtt ttatcgcagg gacggtgggc atcgaacaga tcgagccgct cgcacaacaa ctgccgctgg tgcaggccac gcgcgaagcg aggatcgatc gcgcgttgtc ggtgtcgttc ggtttcgggg ggagttgcgc gagcctgatg tttgccagga tttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa