BuphA.00113.a
Beta-ketoacyl synthase
| CENTER ID: | BuphA.00113.a |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00113.a.A1.GE32278 | Full length( BuphA.00113.a ) | 1 | 408 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00113.a.A1.PS01343 | Full length( BuphA.00113.a ) | 1 | 408 |
Structures
4EWG
DEPOSITED: 4/26/2012
DETERMINATION: XRay
CLONE: BuphA.00113.a.A1.GE32278
PROTEIN: BuphA.00113.a.A1.PS01343
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_205037 |
| PATRIC ID: | fig|391038.7.peg.743 |
| RefSeq: | YP_001856938.1 |
| UniProt: | B2JEW3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRVVITGMG GVTALGSRWD EIEAALKAGR NAVRRMPDWD YFESLHTRLA APLPGFAQPA DWPRKKTRSM GRVSMYAVRA SELALADAGF AGDESISDGR MGVAYGSSSG SVEPIRAFGT MLESGSMTDV TSNSYVQMMP HTTAVNVSLF WDLKGRIVPT SSACASGSQA IGYAYENIAM GKQTLMLAGG AEELSGPAVA VFDTLYATST RNDEPHLTPR PFDAKRDGLV VGEGAATLVL EEYEHAKARG ATIHAEIVGF GCNSDGAHMT QPTASTMARA MQLALEDAKL DANAIAYVNA HGTSTDRGDV AESQATARTF GERMPISSLK SYVGHTLGAC GALEAWWTIE MMKRNWYAPT LNLTEVDPAC APLDYIRGEA RAIDAEYVMS NNFAFGGINT SLIFRRVR
NT Sequence
ATGAAGCGTG TCGTCATTAC GGGCATGGGC GGCGTCACGG CGCTCGGCAG CCGCTGGGAC GAGATCGAAG CGGCACTGAA GGCAGGCCGC AACGCGGTGC GGCGCATGCC GGACTGGGAT TACTTCGAGT CGCTGCATAC GCGTCTCGCG GCGCCGCTGC CTGGGTTCGC TCAACCCGCC GACTGGCCGC GCAAGAAGAC GCGCTCGATG GGCAGGGTAT CGATGTACGC GGTGCGCGCG AGCGAACTCG CGCTCGCCGA TGCGGGTTTC GCGGGGGACG AGTCGATCAG CGACGGCCGC ATGGGCGTCG CATACGGCTC GTCGTCGGGT TCCGTCGAAC CGATCCGCGC ATTCGGCACG ATGCTCGAAT CGGGTTCGAT GACCGACGTC ACGTCGAACA GCTACGTGCA GATGATGCCG CACACGACGG CCGTCAACGT GAGCCTCTTC TGGGATCTGA AGGGGCGTAT CGTGCCGACC TCGTCGGCAT GTGCGTCGGG CAGCCAGGCA ATCGGCTATG CCTACGAAAA TATCGCCATG GGCAAGCAGA CGCTCATGCT CGCGGGCGGC GCGGAAGAAC TGTCGGGGCC GGCCGTCGCC GTGTTCGACA CGCTGTATGC GACCAGCACG CGCAATGACG AGCCGCACCT GACGCCGCGT CCGTTCGACG CGAAGCGCGA CGGTCTCGTG GTCGGCGAGG GCGCGGCGAC GCTCGTGCTC GAAGAATACG AGCACGCGAA GGCGCGCGGC GCGACGATCC ATGCGGAGAT CGTCGGCTTC GGCTGCAATT CGGACGGCGC GCACATGACG CAGCCGACGG CGAGCACGAT GGCGCGCGCG ATGCAGCTCG CGCTCGAAGA CGCAAAGCTC GACGCGAATG CGATTGCCTA TGTGAACGCG CACGGCACGT CGACGGATCG CGGCGACGTG GCGGAGAGCC AGGCCACGGC ACGGACTTTC GGCGAGCGCA TGCCGATCTC GTCGCTGAAG AGCTACGTCG GCCATACGCT TGGTGCATGC GGCGCGCTCG AAGCGTGGTG GACGATCGAA ATGATGAAGC GCAACTGGTA CGCGCCGACG CTCAATTTGA CTGAAGTCGA TCCCGCTTGC GCGCCGCTCG ATTACATTCG AGGCGAGGCA CGCGCGATCG ATGCCGAATA CGTGATGAGC AATAACTTCG CGTTTGGGGG CATCAACACG TCGCTGATTT TCAGGCGCGT TCGA
Details for BuphA.00113.a.A1.GE32278
| HARVESTED ON: | 7/28/2011 |
| SEQUENCED ON: | 8/3/2011 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MKRVVITGMG GVTALGSRWD EIEAALKAGR NAVRRMPDWD YFESLHTRLA APLPGFAQPA DWPRKKTRSM GRVSMYAVRA SELALADAGF AGDESISDGR MGVAYGSSSG SVEPIRAFGT MLESGSMTDV TSNSYVQMMP HTTAVNVSLF WDLKGRIVPT SSACASGSQA IGYAYENIAM GKQTLMLAGG AEELSGPAVA VFDTLYATST RNDEPHLTPR PFDAKRDGLV VGEGAATLVL EEYEHAKARG ATIHAEIVGF GCNSDGAHMT QPTASTMARA MQLALEDAKL DANAIAYVNA HGTSTDRGDV AESQATARTF GERMPISSLK SYVGHTLGAC GALEAWWTIE MMKRNWYAPT LNLTEVDPAC APLDYIRGEA RAIDAEYVMS NNFAFGGINT SLIFRRVR
Validated NT Sequence
ttattatcga acgcgcctga aaatcagcga cgtgttgatg cccccaaacg cgaagttatt gctcatcacg tattcggcat cgatcgcgcg tgcctcgcct cgaatgtaat cgagcggcgc gcaagcggga tcgacttcag tcaaattgag cgtcggcgcg taccagttgc gcttcatcat ttcgatcgtc caccacgctt cgagcgcgcc gcatgcacca agcgtatggc cgacgtagct cttcagcgac gagatcggca tgcgctcgcc gaaagtccgt gccgtggcct ggctctccgc cacgtcgccg cgatccgtcg acgtgccgtg cgcgttcaca taggcaatcg cattcgcgtc gagctttgcg tcttcgagcg cgagctgcat cgcgcgcgcc atcgtgctcg ccgtcggctg cgtcatgtgc gcgccgtccg aattgcagcc gaagccgacg atctccgcat ggatcgtcgc gccgcgcgcc ttcgcgtgct cgtattcttc gagcacgagc gtcgccgcgc cctcgccgac cacgagaccg tcgcgcttcg cgtcgaacgg acgcggcgtc aggtgcggct cgtcattgcg cgtgctggtc gcatacagcg tgtcgaacac ggcgacggcc ggccccgaca gttcttccgc gccgcccgcg agcatgagcg tctgcttgcc catggcgata ttttcgtagg catagccgat tgcctggctg cccgacgcac atgccgacga ggtcggcacg atacgcccct tcagatccca gaagaggctc acgttgacgg ccgtcgtgtg cggcatcatc tgcacgtagc tgttcgacgt gacgtcggtc atcgaacccg attcgagcat cgtgccgaat gcgcggatcg gttcgacgga acccgacgac gagccgtatg cgacgcccat gcggccgtcg ctgatcgact cgtcccccgc gaaacccgca tcggcgagcg cgagttcgct cgcgcgcacc gcgtacatcg ataccctgcc catcgagcgc gtcttcttgc gcggccagtc ggcgggttga gcgaacccag gcagcggcgc cgcgagacgc gtatgcagcg actcgaagta atcccagtcc ggcatgcgcc gcaccgcgtt gcggcctgcc ttcagtgccg cttcgatctc gtcccagcgg ctgccgagcg ccgtgacgcc gcccatgccc gtaatgacga cacgcttcat cgaaccagga ccctgggtct gagcttccag g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKRVVITGM GGVTALGSRW DEIEAALKAG RNAVRRMPDW DYFESLHTRL AAPLPGFAQP ADWPRKKTRS MGRVSMYAVR ASELALADAG FAGDESISDG RMGVAYGSSS GSVEPIRAFG TMLESGSMTD VTSNSYVQMM PHTTAVNVSL FWDLKGRIVP TSSACASGSQ AIGYAYENIA MGKQTLMLAG GAEELSGPAV AVFDTLYATS TRNDEPHLTP RPFDAKRDGL VVGEGAATLV LEEYEHAKAR GATIHAEIVG FGCNSDGAHM TQPTASTMAR AMQLALEDAK LDANAIAYVN AHGTSTDRGD VAESQATART FGERMPISSL KSYVGHTLGA CGALEAWWTI EMMKRNWYAP TLNLTEVDPA CAPLDYIRGE ARAIDAEYVM SNNFAFGGIN TSLIFRRVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagcgtgtc gtcattacgg gcatgggcgg cgtcacggcg ctcggcagcc gctgggacga gatcgaagcg gcactgaagg caggccgcaa cgcggtgcgg cgcatgccgg actgggatta cttcgagtcg ctgcatacgc gtctcgcggc gccgctgcct gggttcgctc aacccgccga ctggccgcgc aagaagacgc gctcgatggg cagggtatcg atgtacgcgg tgcgcgcgag cgaactcgcg ctcgccgatg cgggtttcgc gggggacgag tcgatcagcg acggccgcat gggcgtcgca tacggctcgt cgtcgggttc cgtcgaaccg atccgcgcat tcggcacgat gctcgaatcg ggttcgatga ccgacgtcac gtcgaacagc tacgtgcaga tgatgccgca cacgacggcc gtcaacgtga gcctcttctg ggatctgaag gggcgtatcg tgccgacctc gtcggcatgt gcgtcgggca gccaggcaat cggctatgcc tacgaaaata tcgccatggg caagcagacg ctcatgctcg cgggcggcgc ggaagaactg tcggggccgg ccgtcgccgt gttcgacacg ctgtatgcga ccagcacgcg caatgacgag ccgcacctga cgccgcgtcc gttcgacgcg aagcgcgacg gtctcgtggt cggcgagggc gcggcgacgc tcgtgctcga agaatacgag cacgcgaagg cgcgcggcgc gacgatccat gcggagatcg tcggcttcgg ctgcaattcg gacggcgcgc acatgacgca gccgacggcg agcacgatgg cgcgcgcgat gcagctcgcg ctcgaagacg caaagctcga cgcgaatgcg attgcctatg tgaacgcgca cggcacgtcg acggatcgcg gcgacgtggc ggagagccag gccacggcac ggactttcgg cgagcgcatg ccgatctcgt cgctgaagag ctacgtcggc catacgcttg gtgcatgcgg cgcgctcgaa gcgtggtgga cgatcgaaat gatgaagcgc aactggtacg cgccgacgct caatttgact gaagtcgatc ccgcttgcgc gccgctcgat tacattcgag gcgaggcacg cgcgatcgat gccgaatacg tgatgagcaa taacttcgcg tttgggggca tcaacacgtc gctgattttc aggcgcgttc gataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BuphA.00113.a.A1.PS01343
| PURIFICATION DATe: | 1/12/2012 |
| CONCENTRATION: | 24.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MKRVVITGMG GVTALGSRWD EIEAALKAGR NAVRRMPDWD YFESLHTRLA APLPGFAQPA DWPRKKTRSM GRVSMYAVRA SELALADAGF AGDESISDGR MGVAYGSSSG SVEPIRAFGT MLESGSMTDV TSNSYVQMMP HTTAVNVSLF WDLKGRIVPT SSACASGSQA IGYAYENIAM GKQTLMLAGG AEELSGPAVA VFDTLYATST RNDEPHLTPR PFDAKRDGLV VGEGAATLVL EEYEHAKARG ATIHAEIVGF GCNSDGAHMT QPTASTMARA MQLALEDAKL DANAIAYVNA HGTSTDRGDV AESQATARTF GERMPISSLK SYVGHTLGAC GALEAWWTIE MMKRNWYAPT LNLTEVDPAC APLDYIRGEA RAIDAEYVMS NNFAFGGINT SLIFRRVR
Validated NT Sequence
ttattatcga acgcgcctga aaatcagcga cgtgttgatg cccccaaacg cgaagttatt gctcatcacg tattcggcat cgatcgcgcg tgcctcgcct cgaatgtaat cgagcggcgc gcaagcggga tcgacttcag tcaaattgag cgtcggcgcg taccagttgc gcttcatcat ttcgatcgtc caccacgctt cgagcgcgcc gcatgcacca agcgtatggc cgacgtagct cttcagcgac gagatcggca tgcgctcgcc gaaagtccgt gccgtggcct ggctctccgc cacgtcgccg cgatccgtcg acgtgccgtg cgcgttcaca taggcaatcg cattcgcgtc gagctttgcg tcttcgagcg cgagctgcat cgcgcgcgcc atcgtgctcg ccgtcggctg cgtcatgtgc gcgccgtccg aattgcagcc gaagccgacg atctccgcat ggatcgtcgc gccgcgcgcc ttcgcgtgct cgtattcttc gagcacgagc gtcgccgcgc cctcgccgac cacgagaccg tcgcgcttcg cgtcgaacgg acgcggcgtc aggtgcggct cgtcattgcg cgtgctggtc gcatacagcg tgtcgaacac ggcgacggcc ggccccgaca gttcttccgc gccgcccgcg agcatgagcg tctgcttgcc catggcgata ttttcgtagg catagccgat tgcctggctg cccgacgcac atgccgacga ggtcggcacg atacgcccct tcagatccca gaagaggctc acgttgacgg ccgtcgtgtg cggcatcatc tgcacgtagc tgttcgacgt gacgtcggtc atcgaacccg attcgagcat cgtgccgaat gcgcggatcg gttcgacgga acccgacgac gagccgtatg cgacgcccat gcggccgtcg ctgatcgact cgtcccccgc gaaacccgca tcggcgagcg cgagttcgct cgcgcgcacc gcgtacatcg ataccctgcc catcgagcgc gtcttcttgc gcggccagtc ggcgggttga gcgaacccag gcagcggcgc cgcgagacgc gtatgcagcg actcgaagta atcccagtcc ggcatgcgcc gcaccgcgtt gcggcctgcc ttcagtgccg cttcgatctc gtcccagcgg ctgccgagcg ccgtgacgcc gcccatgccc gtaatgacga cacgcttcat cgaaccagga ccctgggtct gagcttccag g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKRVVITGM GGVTALGSRW DEIEAALKAG RNAVRRMPDW DYFESLHTRL AAPLPGFAQP ADWPRKKTRS MGRVSMYAVR ASELALADAG FAGDESISDG RMGVAYGSSS GSVEPIRAFG TMLESGSMTD VTSNSYVQMM PHTTAVNVSL FWDLKGRIVP TSSACASGSQ AIGYAYENIA MGKQTLMLAG GAEELSGPAV AVFDTLYATS TRNDEPHLTP RPFDAKRDGL VVGEGAATLV LEEYEHAKAR GATIHAEIVG FGCNSDGAHM TQPTASTMAR AMQLALEDAK LDANAIAYVN AHGTSTDRGD VAESQATART FGERMPISSL KSYVGHTLGA CGALEAWWTI EMMKRNWYAP TLNLTEVDPA CAPLDYIRGE ARAIDAEYVM SNNFAFGGIN TSLIFRRVR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGCGTGTC GTCATTACGG GCATGGGCGG CGTCACGGCG CTCGGCAGCC GCTGGGACGA GATCGAAGCG GCACTGAAGG CAGGCCGCAA CGCGGTGCGG CGCATGCCGG ACTGGGATTA CTTCGAGTCG CTGCATACGC GTCTCGCGGC GCCGCTGCCT GGGTTCGCTC AACCCGCCGA CTGGCCGCGC AAGAAGACGC GCTCGATGGG CAGGGTATCG ATGTACGCGG TGCGCGCGAG CGAACTCGCG CTCGCCGATG CGGGTTTCGC GGGGGACGAG TCGATCAGCG ACGGCCGCAT GGGCGTCGCA TACGGCTCGT CGTCGGGTTC CGTCGAACCG ATCCGCGCAT TCGGCACGAT GCTCGAATCG GGTTCGATGA CCGACGTCAC GTCGAACAGC TACGTGCAGA TGATGCCGCA CACGACGGCC GTCAACGTGA GCCTCTTCTG GGATCTGAAG GGGCGTATCG TGCCGACCTC GTCGGCATGT GCGTCGGGCA GCCAGGCAAT CGGCTATGCC TACGAAAATA TCGCCATGGG CAAGCAGACG CTCATGCTCG CGGGCGGCGC GGAAGAACTG TCGGGGCCGG CCGTCGCCGT GTTCGACACG CTGTATGCGA CCAGCACGCG CAATGACGAG CCGCACCTGA CGCCGCGTCC GTTCGACGCG AAGCGCGACG GTCTCGTGGT CGGCGAGGGC GCGGCGACGC TCGTGCTCGA AGAATACGAG CACGCGAAGG CGCGCGGCGC GACGATCCAT GCGGAGATCG TCGGCTTCGG CTGCAATTCG GACGGCGCGC ACATGACGCA GCCGACGGCG AGCACGATGG CGCGCGCGAT GCAGCTCGCG CTCGAAGACG CAAAGCTCGA CGCGAATGCG ATTGCCTATG TGAACGCGCA CGGCACGTCG ACGGATCGCG GCGACGTGGC GGAGAGCCAG GCCACGGCAC GGACTTTCGG CGAGCGCATG CCGATCTCGT CGCTGAAGAG CTACGTCGGC CATACGCTTG GTGCATGCGG CGCGCTCGAA GCGTGGTGGA CGATCGAAAT GATGAAGCGC AACTGGTACG CGCCGACGCT CAATTTGACT GAAGTCGATC CCGCTTGCGC GCCGCTCGAT TACATTCGAG GCGAGGCACG CGCGATCGAT GCCGAATACG TGATGAGCAA TAACTTCGCG TTTGGGGGCA TCAACACGTC GCTGATTTTC AGGCGCGTTC GATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA