SSGCID: Target Page

BuphA.00109.b

N-isopropylammelide isopropylaminohydrolase

CENTER ID:	BuphA.00109.b
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00109.b.B1.GE40193	Full length( BuphA.00109.b )		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.5617
RefSeq:	YP_001861464.1
UniProt:	B2JNB6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKDTMLLTDV RMPDGVRVDV AIAEGRIAAI GPDLQVKHEV AREHGNGALL LPGFVEGHTH LDKTMWGMRW YRNEVGPTLT DRIDNERRFR AQSGHDAAAA SLALAHAFLQ MGTTRLRTHV DIDTDAGLKH LEGVLTTRDA LGDVLDMQTV AFPQSGMLIR EGTIALLDDA LRAGADVLGA LDPALIDGDP VASLNATFDL ASRHHKPIDI HLHEPAEIGG FTLKLLLDRV EALGMQGRVV ISHAFCLGAL APRERDPLLE RLARLDVALL TTAPASVTVP PLAACIEKGV TLFGGNDGIR DTWNPFGSPD MLERAAQIAM RYDLRRDDAI AVAFDCVSHA AARGCQFTDY GLHVGARADL VLVDAETVAH AVAARPVRQL VVANGRVVAR HGRLADTL

NT Sequence

ATGAAAGACA CGATGTTGCT TACCGATGTC CGCATGCCGG ACGGCGTGCG CGTCGATGTC GCGATCGCCG AGGGCCGCAT CGCTGCGATC GGTCCCGATT TGCAGGTCAA ACATGAGGTG GCGCGCGAGC ACGGCAACGG TGCGTTGCTG CTGCCCGGTT TTGTAGAAGG CCATACGCAT CTCGACAAGA CCATGTGGGG CATGCGCTGG TATCGCAACG AAGTGGGACC CACGCTCACC GATCGCATCG ACAACGAGCG GCGCTTTCGA GCGCAGAGCG GACACGACGC GGCGGCGGCA TCGCTTGCGT TGGCGCACGC GTTCCTGCAA ATGGGCACCA CGCGTTTGCG CACGCACGTC GATATCGACA CGGATGCGGG GCTCAAGCAC CTCGAAGGCG TACTGACGAC ACGCGACGCG CTCGGCGACG TCCTCGACAT GCAGACCGTT GCGTTTCCAC AGTCGGGCAT GCTCATCCGT GAGGGCACCA TCGCGTTGCT CGACGATGCA TTGCGTGCGG GCGCCGACGT GCTCGGCGCG CTGGACCCAG CGCTGATCGA CGGCGATCCC GTCGCTTCGC TGAACGCCAC CTTCGATCTC GCCTCGCGCC ATCACAAGCC GATCGATATT CATCTGCACG AGCCCGCCGA AATCGGCGGT TTCACATTGA AGCTGCTGCT CGACCGTGTC GAAGCGCTCG GCATGCAGGG ACGCGTCGTG ATCAGCCATG CGTTCTGTCT CGGCGCGCTG GCACCGCGCG AGCGCGATCC GCTGCTCGAA CGGCTCGCAC GACTCGACGT CGCGTTGCTC ACGACGGCGC CCGCCTCCGT TACCGTGCCG CCGCTAGCCG CGTGCATCGA AAAAGGTGTC ACGCTGTTCG GCGGCAACGA CGGCATCCGC GACACCTGGA ATCCGTTTGG CTCGCCCGAC ATGCTGGAGC GCGCGGCGCA GATCGCGATG CGCTACGACC TGCGCCGCGA CGATGCGATC GCCGTCGCGT TCGATTGCGT GAGCCACGCC GCCGCTCGCG GTTGCCAGTT CACCGACTAC GGCCTGCATG TGGGCGCACG TGCCGATCTC GTCCTCGTCG ATGCGGAAAC CGTCGCGCAT GCCGTTGCGG CGCGGCCCGT GCGCCAGCTG GTGGTGGCGA ACGGACGCGT TGTTGCGCGT CACGGAAGGC TGGCCGACAC GCTC

Details for BuphA.00109.b.B1.GE40193

HARVESTED ON:	5/9/2016
SEQUENCED ON:	5/23/2016
EXPECTED MW:	44kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMK DTMLLTDVRM PDGVRVDVAI AEGRIAAIGP DLQVKHEVAR EHGNGALLLP GFVEGHTHLD KTMWGMRWYR NEVGPTLTDR IDNERRFRAQ SGHDAAAASL ALAHAFLQMG TTRLRTHVDI DTDAGLKHLE GVLTTRDALG DVLDMQTVAF PQSGMLIREG TIALLDDALR AGADVLGALD PALIDGDPVA SLNATFDLAS RHHKPIDIHL HEPAEIGGFT LKLLLDRVEA LGMQGRVVIS HAFCLGALAP RERDPLLERL ARLDVALLTT APASVTVPPL AACIEKGVTL FGGNDGIRDT WNPFGSPDML ERAAQIAMRY DLRRDDAIAV AFDCVSHAAA RGCQFTDYGL HVGARADLVL VDAETVAHAV AARPVRQLVV ANGRVVARX

Validated NT Sequence

cgnnncgcgc aacaacgcgt ccgttcgcca ccaccagctg gcgcacgggc cgcgccgcaa cggcatgcgc gacggtttcc gcatcgacga ggacgagatc ggcacgtgcg cccacatgca ggccgtagtc ggtgaactgg caaccgcgag cggcggcgtg gctcacgcaa tcgaacgcga cggcgatcgc atcgtcgcgg cgcaggtcgt agcgcatcgc gatctgcgcc gcgcgctcca gcatgtcggg cgagccaaac ggattccagg tgtcgcggat gccgtcgttg ccgccgaaca gcgtgacacc tttttcgatg cacgcggcta gcggcggcac ggtaacggag gcgggcgccg tcgtgagcaa cgcgacgtcg agtcgtgcga gccgttcgag cagcggatcg cgctcgcgcg gtgccagcgc gccgagacag aacgcatggc tgatcacgac gcgtccctgc atgccgagcg cttcgacacg gtcgagcagc agcttcaatg tgaaaccgcc gatttcggcg ggctcgtgca gatgaatatc gatcggcttg tgatggcgcg aggcgagatc gaaggtggcg ttcagcgaag cgacgggatc gccgtcgatc agcgctgggt ccagcgcgcc gagcacgtcg gcgcccgcac gcaatgcatc gtcgagcaac gcgatggtgc cctcacggat gagcatgccc gactgtggaa acgcaacggt ctgcatgtcg aggacgtcgc cgagcgcgtc gcgtgtcgtc agtacgcctt cgaggtgctt gagccccgca tccgtgtcga tatcgacgtg cgtgcgcaaa cgcgtggtgc ccatttgcag gaacgcgtgc gccaacgcaa gcgatgccgc cgccgcgtcg tgtccgctct gcgctcgaaa gcgccgctcg ttgtcgatgc gatcggtgag cgtgggtccc acttcgttgc gataccagcg catgccccac atggtcttgt cgagatgcgt atggccttct acaaaaccgg gcagcagcaa cgcaccgttg ccgtgctcgc gcgccacctc atgtttgacc tgcaaatcgg gaccgatcgc agcgatgcgg ccctcggcga tcgcgacatc gacgcgcacg ccgtccggca tgcggacatc ggtaagcaac atcgtgtctt tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag acacgatgtt gcttaccgat gtccgcatgc cggacggcgt gcgcgtcgat gtcgcgatcg ccgagggccg catcgctgcg atcggtcccg atttgcaggt caaacatgag gtggcgcgcg agcacggcaa cggtgcgttg ctgctgcccg gttttgtaga aggccatacg catctcgaca agaccatgtg gggcatgcgc tggtatcgca acgaagtggg acccacgctc accgatcgca tcgacaacga gcggcgcttt cgagcgcaga gcggacacga cgcggcggcg gcatcgcttg cgttggcgca cgcgttcctg caaatgggca ccacgcgttt gcgcacgcac gtcgatatcg acacggatgc ggggctcaag cacctcgaag gcgtactgac gacacgcgac gcgctcggcg acgtcctcga catgcagacc gttgcgtttc cacagtcggg catgctcatc cgtgagggca ccatcgcgtt gctcgacgat gcattgcgtg cgggcgccga cgtgctcggc gcgctggacc cagcgctgat cgacggcgat cccgtcgctt cgctgaacgc caccttcgat ctcgcctcgc gccatcacaa gccgatcgat attcatctgc acgagcccgc cgaaatcggc ggtttcacat tgaagctgct gctcgaccgt gtcgaagcgc tcggcatgca gggacgcgtc gtgatcagcc atgcgttctg tctcggcgcg ctggcaccgc gcgagcgcga tccgctgctc gaacggctcg cacgactcga cgtcgcgttg ctcacgacgg cgcccgcctc cgttaccgtg ccgccgctag ccgcgtgcat cgaaaaaggt gtcacgctgt tcggcggcaa cgacggcatc cgcgacacct ggaatccgtt tggctcgccc gacatgctgg agcgcgcggc gcagatcgcg atgcgctacg acctgcgccg cgacgatgcg atcgccgtcg cgttcgattg cgtgagccac gccgccgctc gcggttgcca gttcaccgac tacggcctgc atgtgggcgc acgtgccgat ctcgtcctcg tcgatgcgga aaccgtcgcg catgccgttg cggcgcggcc cgtgcgccag ctggtggtgg cgaacggacg cgttgttgcg cgtcacggaa ggctggccga cacgctctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at