BuphA.00107.g

Efflux transporter, RND family, MFP subunit

CENTER ID: BuphA.00107.g
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00107.g.A2.GE32277 Mature protein( BuphA.00107.g ) 33 383
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00107.g.A2.PS01375 Mature protein( BuphA.00107.g ) 33 383

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.7971
RefSeq: YP_001863648.1
UniProt: B2JY90

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTKRPMTRR MIIMLIFVCL LLSALVGFNL FRAHMIKKFM ASNAVPPATV TAAVAGYQTW QPQLAAVGSL RAVRGVDVTT EVAGLVREIA FSSGQEVKAG QVLVRLNDDS DRAQLASLQA AAELAQTVYK RDKAQFDIQA IAKAQLDADA ADLKSKKAQV DQQAALVEKK TIRAPFAGRV GITTVNPGQY INPGDAIVTL QAIDPIYADF YLPQQQLGQL QVGQAIVVDT NAFSNRTFDG KIRSINPRVD STTRNVQIEA TVDNRERKLL PGMYAKVKID AGAVQRYLTL PQTAITYNPY GATVFVVKPG AQKNAQNKIM PVAQQVFVAP GPTRGDQVAI LKGIGEGAQV VTSGQLKLKN GTPLVIDNRV LPADNPNPTP QEQ
NT Sequence
ATGACAACGA AGAGACCCAT GACCAGGCGG ATGATCATCA TGCTGATCTT CGTGTGCTTG CTGCTCAGTG CGCTGGTCGG CTTCAATCTG TTCAGGGCGC ACATGATCAA AAAGTTCATG GCGAGCAATG CCGTGCCGCC CGCGACCGTC ACGGCCGCTG TCGCAGGCTA TCAGACGTGG CAGCCGCAGC TTGCCGCCGT GGGCAGCCTG CGCGCGGTGC GCGGCGTCGA CGTGACGACG GAAGTGGCGG GGCTCGTGCG CGAAATCGCA TTCAGCTCGG GCCAGGAAGT GAAGGCAGGC CAGGTGCTCG TGCGCCTGAA CGACGATTCG GACCGCGCGC AGCTCGCGTC GCTGCAGGCC GCGGCGGAAC TCGCGCAAAC CGTCTATAAG CGCGACAAGG CTCAGTTCGA CATTCAGGCG ATTGCGAAGG CGCAACTCGA CGCCGATGCC GCCGACCTGA AGAGCAAGAA AGCCCAGGTC GACCAGCAGG CCGCGCTCGT CGAAAAGAAA ACGATACGCG CGCCCTTCGC GGGACGCGTC GGCATCACGA CGGTCAACCC GGGCCAGTAC ATCAATCCGG GCGATGCGAT CGTCACGCTG CAAGCCATCG ATCCGATCTA CGCCGACTTC TATCTGCCGC AACAGCAACT CGGACAATTG CAGGTCGGCC AGGCGATCGT CGTCGATACG AATGCGTTCA GCAACCGCAC GTTCGACGGC AAGATCCGCT CGATCAATCC GCGCGTGGAC AGCACGACGC GTAACGTGCA GATCGAAGCG ACCGTCGACA ACCGCGAGCG CAAGCTGCTG CCCGGCATGT ACGCGAAAGT GAAGATCGAC GCGGGCGCCG TGCAGCGCTA TCTGACCTTG CCGCAAACGG CGATCACCTA TAACCCGTAC GGCGCGACCG TGTTCGTCGT GAAGCCCGGC GCGCAAAAGA ATGCGCAAAA CAAGATCATG CCCGTCGCGC AGCAGGTGTT CGTGGCGCCG GGGCCGACGC GCGGCGATCA GGTCGCGATT CTCAAGGGCA TCGGCGAAGG CGCGCAGGTC GTGACGAGCG GCCAGTTGAA ACTGAAGAAC GGCACGCCGC TCGTGATCGA CAACCGCGTG CTGCCCGCGG ACAACCCGAA TCCGACGCCT CAGGAACAA
Details for BuphA.00107.g.A2.GE32277
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MAHMIKKFMA SNAVPPATVT AAVAGYQTWQ PQLAAVGSLR AVRGVDVTTE VAGLVREIAF SSGQEVKAGQ VLVRLNDDSD RAQLASLQAA AELAQTVYKR DKAQFDIQAI AKAQLDADAA DLKSKKAQVD QQAALVEKKT IRAPFAGRVG ITTVNPGQYI NPGDAIVTLQ AIDPIYADFY LPQQQLGQLQ VGQAIVVDTN AFSNRTFDGK IRSINPRVDS TTRNVQIEAT VDNRERKLLP GMYAKVKIDA GAVQRYLTLP QTAITYNPYG ATVFVVKPGA QKNAQNKIMP VAQQVFVAPG PTRGDQVAIL KGIGEGAQVV TSGQLKLKNG TPLVIDNRVL PADNPNPTPQ
Validated NT Sequence
cctgaggcgt cggattcggg ttgtccgcgg gcagcacgcg gttgtcgatc acgagcggcg tgccgttctt cagtttcaac tggccgctcg tcacgacctg cgcgccttcg ccgatgccct tgagaatcgc gacctgatcg ccgcgcgtcg gccccggcgc cacgaacacc tgctgcgcga cgggcatgat cttgttttgc gcattctttt gcgcgccggg cttcacgacg aacacggtcg cgccgtacgg gttataggtg atcgccgttt gcggcaaggt cagatagcgc tgcacggcgc ccgcgtcgat cttcactttc gcgtacatgc cgggcagcag cttgcgctcg cggttgtcga cggtcgcttc gatctgcacg ttacgcgtcg tgctgtccac gcgcggattg atcgagcgga tcttgccgtc gaacgtgcgg ttgctgaacg cattcgtatc gacgacgatc gcctggccga cctgcaattg tccgagttgc tgttgcggca gatagaagtc ggcgtagatc ggatcgatgg cttgcagcgt gacgatcgca tcgcccggat tgatgtactg gcccgggttg accgtcgtga tgccgacgcg tcccgcgaag ggcgcgcgta tcgttttctt ttcgacgagc gcggcctgct ggtcgacctg ggctttcttg ctcttcaggt cggcggcatc ggcgtcgagt tgcgccttcg caatcgcctg aatgtcgaac tgagccttgt cgcgcttata gacggtttgc gcgagttccg ccgcggcctg cagcgacgcg agctgcgcgc ggtccgaatc gtcgttcagg cgcacgagca cctggcctgc cttcacttcc tggcccgagc tgaatgcgat ttcgcgcacg agccccgcca cttccgtcgt cacgtcgacg ccgcgcaccg cgcgcaggct gcccacggcg gcaagctgcg gctgccacgt ctgatagcct gcgacagcgg ccgtgacggt cgcgggcggc acggcattgc tcgccatgaa ctttttgatc atgtgcgcca tcgaaccagg accctgggtc tgagcttcca gg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAHMIKKFM ASNAVPPATV TAAVAGYQTW QPQLAAVGSL RAVRGVDVTT EVAGLVREIA FSSGQEVKAG QVLVRLNDDS DRAQLASLQA AAELAQTVYK RDKAQFDIQA IAKAQLDADA ADLKSKKAQV DQQAALVEKK TIRAPFAGRV GITTVNPGQY INPGDAIVTL QAIDPIYADF YLPQQQLGQL QVGQAIVVDT NAFSNRTFDG KIRSINPRVD STTRNVQIEA TVDNRERKLL PGMYAKVKID AGAVQRYLTL PQTAITYNPY GATVFVVKPG AQKNAQNKIM PVAQQVFVAP GPTRGDQVAI LKGIGEGAQV VTSGQLKLKN GTPLVIDNRV LPADNPNPTP QEQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcacatg atcaaaaagt tcatggcgag caatgccgtg ccgcccgcga ccgtcacggc cgctgtcgca ggctatcaga cgtggcagcc gcagcttgcc gccgtgggca gcctgcgcgc ggtgcgcggc gtcgacgtga cgacggaagt ggcggggctc gtgcgcgaaa tcgcattcag ctcgggccag gaagtgaagg caggccaggt gctcgtgcgc ctgaacgacg attcggaccg cgcgcagctc gcgtcgctgc aggccgcggc ggaactcgcg caaaccgtct ataagcgcga caaggctcag ttcgacattc aggcgattgc gaaggcgcaa ctcgacgccg atgccgccga cctgaagagc aagaaagccc aggtcgacca gcaggccgcg ctcgtcgaaa agaaaacgat acgcgcgccc ttcgcgggac gcgtcggcat cacgacggtc aacccgggcc agtacatcaa tccgggcgat gcgatcgtca cgctgcaagc catcgatccg atctacgccg acttctatct gccgcaacag caactcggac aattgcaggt cggccaggcg atcgtcgtcg atacgaatgc gttcagcaac cgcacgttcg acggcaagat ccgctcgatc aatccgcgcg tggacagcac gacgcgtaac gtgcagatcg aagcgaccgt cgacaaccgc gagcgcaagc tgctgcccgg catgtacgcg aaagtgaaga tcgacgcggg cgccgtgcag cgctatctga ccttgccgca aacggcgatc acctataacc cgtacggcgc gaccgtgttc gtcgtgaagc ccggcgcgca aaagaatgcg caaaacaaga tcatgcccgt cgcgcagcag gtgttcgtgg cgccggggcc gacgcgcggc gatcaggtcg cgattctcaa gggcatcggc gaaggcgcgc aggtcgtgac gagcggccag ttgaaactga agaacggcac gccgctcgtg atcgacaacc gcgtgctgcc cgcggacaac ccgaatccga cgcctcagga acaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BuphA.00107.g.A2.PS01375
PURIFICATION DATe: 2/1/2012
CONCENTRATION: 25.04mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MAHMIKKFMA SNAVPPATVT AAVAGYQTWQ PQLAAVGSLR AVRGVDVTTE VAGLVREIAF SSGQEVKAGQ VLVRLNDDSD RAQLASLQAA AELAQTVYKR DKAQFDIQAI AKAQLDADAA DLKSKKAQVD QQAALVEKKT IRAPFAGRVG ITTVNPGQYI NPGDAIVTLQ AIDPIYADFY LPQQQLGQLQ VGQAIVVDTN AFSNRTFDGK IRSINPRVDS TTRNVQIEAT VDNRERKLLP GMYAKVKIDA GAVQRYLTLP QTAITYNPYG ATVFVVKPGA QKNAQNKIMP VAQQVFVAPG PTRGDQVAIL KGIGEGAQVV TSGQLKLKNG TPLVIDNRVL PADNPNPTPQ
Validated NT Sequence
cctgaggcgt cggattcggg ttgtccgcgg gcagcacgcg gttgtcgatc acgagcggcg tgccgttctt cagtttcaac tggccgctcg tcacgacctg cgcgccttcg ccgatgccct tgagaatcgc gacctgatcg ccgcgcgtcg gccccggcgc cacgaacacc tgctgcgcga cgggcatgat cttgttttgc gcattctttt gcgcgccggg cttcacgacg aacacggtcg cgccgtacgg gttataggtg atcgccgttt gcggcaaggt cagatagcgc tgcacggcgc ccgcgtcgat cttcactttc gcgtacatgc cgggcagcag cttgcgctcg cggttgtcga cggtcgcttc gatctgcacg ttacgcgtcg tgctgtccac gcgcggattg atcgagcgga tcttgccgtc gaacgtgcgg ttgctgaacg cattcgtatc gacgacgatc gcctggccga cctgcaattg tccgagttgc tgttgcggca gatagaagtc ggcgtagatc ggatcgatgg cttgcagcgt gacgatcgca tcgcccggat tgatgtactg gcccgggttg accgtcgtga tgccgacgcg tcccgcgaag ggcgcgcgta tcgttttctt ttcgacgagc gcggcctgct ggtcgacctg ggctttcttg ctcttcaggt cggcggcatc ggcgtcgagt tgcgccttcg caatcgcctg aatgtcgaac tgagccttgt cgcgcttata gacggtttgc gcgagttccg ccgcggcctg cagcgacgcg agctgcgcgc ggtccgaatc gtcgttcagg cgcacgagca cctggcctgc cttcacttcc tggcccgagc tgaatgcgat ttcgcgcacg agccccgcca cttccgtcgt cacgtcgacg ccgcgcaccg cgcgcaggct gcccacggcg gcaagctgcg gctgccacgt ctgatagcct gcgacagcgg ccgtgacggt cgcgggcggc acggcattgc tcgccatgaa ctttttgatc atgtgcgcca tcgaaccagg accctgggtc tgagcttcca gg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAHMIKKFM ASNAVPPATV TAAVAGYQTW QPQLAAVGSL RAVRGVDVTT EVAGLVREIA FSSGQEVKAG QVLVRLNDDS DRAQLASLQA AAELAQTVYK RDKAQFDIQA IAKAQLDADA ADLKSKKAQV DQQAALVEKK TIRAPFAGRV GITTVNPGQY INPGDAIVTL QAIDPIYADF YLPQQQLGQL QVGQAIVVDT NAFSNRTFDG KIRSINPRVD STTRNVQIEA TVDNRERKLL PGMYAKVKID AGAVQRYLTL PQTAITYNPY GATVFVVKPG AQKNAQNKIM PVAQQVFVAP GPTRGDQVAI LKGIGEGAQV VTSGQLKLKN GTPLVIDNRV LPADNPNPTP QEQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCACATG ATCAAAAAGT TCATGGCGAG CAATGCCGTG CCGCCCGCGA CCGTCACGGC CGCTGTCGCA GGCTATCAGA CGTGGCAGCC GCAGCTTGCC GCCGTGGGCA GCCTGCGCGC GGTGCGCGGC GTCGACGTGA CGACGGAAGT GGCGGGGCTC GTGCGCGAAA TCGCATTCAG CTCGGGCCAG GAAGTGAAGG CAGGCCAGGT GCTCGTGCGC CTGAACGACG ATTCGGACCG CGCGCAGCTC GCGTCGCTGC AGGCCGCGGC GGAACTCGCG CAAACCGTCT ATAAGCGCGA CAAGGCTCAG TTCGACATTC AGGCGATTGC GAAGGCGCAA CTCGACGCCG ATGCCGCCGA CCTGAAGAGC AAGAAAGCCC AGGTCGACCA GCAGGCCGCG CTCGTCGAAA AGAAAACGAT ACGCGCGCCC TTCGCGGGAC GCGTCGGCAT CACGACGGTC AACCCGGGCC AGTACATCAA TCCGGGCGAT GCGATCGTCA CGCTGCAAGC CATCGATCCG ATCTACGCCG ACTTCTATCT GCCGCAACAG CAACTCGGAC AATTGCAGGT CGGCCAGGCG ATCGTCGTCG ATACGAATGC GTTCAGCAAC CGCACGTTCG ACGGCAAGAT CCGCTCGATC AATCCGCGCG TGGACAGCAC GACGCGTAAC GTGCAGATCG AAGCGACCGT CGACAACCGC GAGCGCAAGC TGCTGCCCGG CATGTACGCG AAAGTGAAGA TCGACGCGGG CGCCGTGCAG CGCTATCTGA CCTTGCCGCA AACGGCGATC ACCTATAACC CGTACGGCGC GACCGTGTTC GTCGTGAAGC CCGGCGCGCA AAAGAATGCG CAAAACAAGA TCATGCCCGT CGCGCAGCAG GTGTTCGTGG CGCCGGGGCC GACGCGCGGC GATCAGGTCG CGATTCTCAA GGGCATCGGC GAAGGCGCGC AGGTCGTGAC GAGCGGCCAG TTGAAACTGA AGAACGGCAC GCCGCTCGTG ATCGACAACC GCGTGCTGCC CGCGGACAAC CCGAATCCGA CGCCTCAGGA ACAATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA