BuphA.00107.f

Efflux transporter, RND family, MFP subunit

CENTER ID: BuphA.00107.f
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00107.f.A2.GE34736 Mature protein( BuphA.00107.f ) 28 429
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00107.f.A2.PW35346 Mature protein( BuphA.00107.f ) 28 429

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4895
RefSeq: YP_001860790.1
UniProt: B2JR78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVFPLSRSK LAVAALAVIV IAGLGTFGAI RVDARSAGQS APTIVPEVDV ATVIQKTITD WQSYSGRLQA VEKVEVRPLV SGTIVSVNFQ DGALVKKGDV LFVIDPRPYQ AEVDRAAAQL AAAQSRAGYA QSDWERAQRL IGDSAIARRD YDEKQNAARE ANANVKAAQA ALEAAQINLG YTKIVAPVSG RVSRAEITLG NVVNAGASAA PLTTLVSVSP IYAEFDADEQ TYLQYISQVK SDSKVPVELG LANETGYSRK GTIQSVDNRL DTSSGTIRVR ARFDNADGSL VPGLYARVKV GGSEPHSALL IDDAAIGTDQ DKKFVFVVDK DDHVGYREIQ LGDLQGSLRV VKGGLHAGDR IVVNGTQRVR PGAQIRAHMV PMDAGNGNSA PVADDAQADK QAQLDGSKQH AEPQKQAQAQ PHAKTRKDS
NT Sequence
ATGTCTGTTT TTCCTCTCTC TCGTTCGAAG CTGGCGGTTG CGGCGCTGGC CGTGATCGTC ATCGCGGGCC TCGGCACGTT CGGCGCGATC CGCGTGGATG CACGCTCAGC CGGGCAATCC GCGCCGACCA TCGTGCCGGA AGTCGACGTC GCGACCGTCA TTCAGAAAAC CATCACCGAC TGGCAAAGCT ATTCGGGCCG TCTTCAGGCA GTGGAAAAGG TCGAGGTGCG CCCGCTCGTC TCTGGCACCA TCGTGTCCGT GAACTTCCAG GACGGCGCGC TCGTGAAGAA GGGCGACGTG CTCTTCGTGA TCGATCCGCG CCCGTATCAG GCGGAAGTCG ATCGCGCCGC GGCACAGCTC GCCGCTGCGC AGTCGCGCGC AGGCTACGCG CAAAGCGACT GGGAGCGTGC GCAACGGCTG ATCGGCGATA GCGCGATTGC CAGGCGCGAT TACGACGAGA AGCAGAATGC GGCGCGCGAA GCGAACGCGA ATGTGAAGGC CGCGCAAGCG GCGCTCGAAG CGGCGCAGAT CAACCTCGGC TATACGAAGA TCGTCGCGCC CGTGTCGGGC CGCGTGTCGC GTGCCGAGAT CACGCTCGGC AATGTCGTGA ACGCGGGCGC GAGCGCCGCG CCGCTGACGA CGCTCGTGTC GGTGTCGCCG ATCTACGCGG AGTTCGACGC CGACGAGCAG ACGTATCTTC AGTACATCAG CCAGGTGAAG AGCGACAGCA AGGTGCCCGT CGAACTGGGC CTCGCGAACG AAACGGGCTA TTCGCGCAAG GGCACGATCC AGTCGGTCGA CAACCGGCTG GATACGTCGT CAGGCACGAT CCGCGTGCGC GCACGTTTCG ACAACGCTGA CGGCTCGCTG GTCCCAGGCC TCTATGCACG CGTGAAAGTG GGCGGCAGCG AGCCGCATTC GGCGCTGCTG ATCGACGATG CGGCGATCGG CACGGACCAG GACAAGAAGT TCGTGTTCGT CGTCGACAAA GACGATCACG TCGGCTACCG CGAAATCCAG TTGGGCGATC TGCAGGGCAG TCTGCGCGTC GTCAAGGGTG GGCTGCACGC GGGCGACCGC ATCGTCGTGA ACGGCACGCA GCGAGTGCGC CCCGGTGCGC AGATCCGGGC GCACATGGTG CCGATGGACG CAGGCAATGG GAACAGCGCG CCCGTCGCCG ACGACGCGCA GGCGGACAAG CAGGCGCAAC TCGACGGATC GAAGCAGCAC GCCGAACCGC AAAAGCAGGC ACAGGCGCAG CCGCACGCGA AGACACGCAA GGATTCG
Details for BuphA.00107.f.A2.GE34736
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAIRVDAR SAGQSAPTIV PEVDVATVIQ KTITDWQSYS GRLQAVEKVE VRPLVSGTIV SVNFQDGALV KKGDVLFVID PRPYQAEVDR AAAQLAAAQS RAGYAQSDWE RAQRLIGDSA IARRDYDEKQ NAAREANANV KAAQAALEAA QINLGYTKIV APVSGRVSRA EITLGNVVNA GASAAPLTTL VSVSPIYAEF DADEQTYLQY ISQVKSDSKV PVELGLANET GYSRKGTIQS VDNRLDTSSG TIRVRARFDN ADGSLVPGLY ARVKVGGSEP HSALLIDDAA IGTDQDKKFV FVVDKDDHVG YREIQLGDLQ GSLRVVKGGL HAGDRIVVNG TQRVRPGAQI RAHMVPMDAG NGNSAPVADD AQADKQAQLD GSKQHAEPQK QAQAQPHAKT RK
Validated NT Sequence
ccttgcgtgt cttcgcgtgc ggctgcgcct gtgcctgctt ttgcggttcg gcgtgctgct tcgatccgtc gagttgcgcc tgcttgtccg cctgcgcgtc gtcggcgacg ggcgcgctgt tcccattgcc tgcgtccatc ggcaccatgt gcgcccggat ctgcgcaccg gggcgcactc gctgcgtgcc gttcacgacg atgcggtcgc ccgcgtgcag cccacccttg acgacgcgca gactgccctg cagatcgccc aactggattt cgcggtagcc gacgtgatcg tctttgtcga cgacgaacac gaacttcttg tcctggtccg tgccgatcgc cgcatcgtcg atcagcagcg ccgaatgcgg ctcgctgccg cccactttca cgcgtgcata gaggcctggg accagcgagc cgtcagcgtt gtcgaaacgt gcgcgcacgc ggatcgtgcc tgacgacgta tccagccggt tgtcgaccga ctggatcgtg cccttgcgcg aatagcccgt ttcgttcgcg aggcccagtt cgacgggcac cttgctgtcg ctcttcacct ggctgatgta ctgaagatac gtctgctcgt cggcgtcgaa ctccgcgtag atcggcgaca ccgacacgag cgtcgtcagc ggcgcggcgc tcgcgcccgc gttcacgaca ttgccgagcg tgatctcggc acgcgacacg cggcccgaca cgggcgcgac gatcttcgta tagccgaggt tgatctgcgc cgcttcgagc gccgcttgcg cggccttcac attcgcgttc gcttcgcgcg ccgcattctg cttctcgtcg taatcgcgcc tggcaatcgc gctatcgccg atcagccgtt gcgcacgctc ccagtcgctt tgcgcgtagc ctgcgcgcga ctgcgcagcg gcgagctgtg ccgcggcgcg atcgacttcc gcctgatacg ggcgcggatc gatcacgaag agcacgtcgc ccttcttcac gagcgcgccg tcctggaagt tcacggacac gatggtgcca gagacgagcg ggcgcacctc gaccttttcc actgcctgaa gacggcccga atagctttgc cagtcggtga tggttttctg aatgacggtc gcgacgtcga cttccggcac gatggtcggc gcggattgcc cggctgagcg tgcatccacg cggatcgcgc ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAIRVDAR SAGQSAPTIV PEVDVATVIQ KTITDWQSYS GRLQAVEKVE VRPLVSGTIV SVNFQDGALV KKGDVLFVID PRPYQAEVDR AAAQLAAAQS RAGYAQSDWE RAQRLIGDSA IARRDYDEKQ NAAREANANV KAAQAALEAA QINLGYTKIV APVSGRVSRA EITLGNVVNA GASAAPLTTL VSVSPIYAEF DADEQTYLQY ISQVKSDSKV PVELGLANET GYSRKGTIQS VDNRLDTSSG TIRVRARFDN ADGSLVPGLY ARVKVGGSEP HSALLIDDAA IGTDQDKKFV FVVDKDDHVG YREIQLGDLQ GSLRVVKGGL HAGDRIVVNG TQRVRPGAQI RAHMVPMDAG NGNSAPVADD AQADKQAQLD GSKQHAEPQK QAQAQPHAKT RKDS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgcgatc cgcgtggatg cacgctcagc cgggcaatcc gcgccgacca tcgtgccgga agtcgacgtc gcgaccgtca ttcagaaaac catcaccgac tggcaaagct attcgggccg tcttcaggca gtggaaaagg tcgaggtgcg cccgctcgtc tctggcacca tcgtgtccgt gaacttccag gacggcgcgc tcgtgaagaa gggcgacgtg ctcttcgtga tcgatccgcg cccgtatcag gcggaagtcg atcgcgccgc ggcacagctc gccgctgcgc agtcgcgcgc aggctacgcg caaagcgact gggagcgtgc gcaacggctg atcggcgata gcgcgattgc caggcgcgat tacgacgaga agcagaatgc ggcgcgcgaa gcgaacgcga atgtgaaggc cgcgcaagcg gcgctcgaag cggcgcagat caacctcggc tatacgaaga tcgtcgcgcc cgtgtcgggc cgcgtgtcgc gtgccgagat cacgctcggc aatgtcgtga acgcgggcgc gagcgccgcg ccgctgacga cgctcgtgtc ggtgtcgccg atctacgcgg agttcgacgc cgacgagcag acgtatcttc agtacatcag ccaggtgaag agcgacagca aggtgcccgt cgaactgggc ctcgcgaacg aaacgggcta ttcgcgcaag ggcacgatcc agtcggtcga caaccggctg gatacgtcgt caggcacgat ccgcgtgcgc gcacgtttcg acaacgctga cggctcgctg gtcccaggcc tctatgcacg cgtgaaagtg ggcggcagcg agccgcattc ggcgctgctg atcgacgatg cggcgatcgg cacggaccag gacaagaagt tcgtgttcgt cgtcgacaaa gacgatcacg tcggctaccg cgaaatccag ttgggcgatc tgcagggcag tctgcgcgtc gtcaagggtg ggctgcacgc gggcgaccgc atcgtcgtga acggcacgca gcgagtgcgc cccggtgcgc agatccgggc gcacatggtg ccgatggacg caggcaatgg gaacagcgcg cccgtcgccg acgacgcgca ggcggacaag caggcgcaac tcgacggatc gaagcagcac gccgaaccgc aaaagcaggc acaggcgcag ccgcacgcga agacacgcaa ggattcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuphA.00107.f.A2.PW35346
PURIFICATION DATe: 4/6/2012
CONCENTRATION: 27.1mg/ml
OBSERVED MW: 43kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAIRVDAR SAGQSAPTIV PEVDVATVIQ KTITDWQSYS GRLQAVEKVE VRPLVSGTIV SVNFQDGALV KKGDVLFVID PRPYQAEVDR AAAQLAAAQS RAGYAQSDWE RAQRLIGDSA IARRDYDEKQ NAAREANANV KAAQAALEAA QINLGYTKIV APVSGRVSRA EITLGNVVNA GASAAPLTTL VSVSPIYAEF DADEQTYLQY ISQVKSDSKV PVELGLANET GYSRKGTIQS VDNRLDTSSG TIRVRARFDN ADGSLVPGLY ARVKVGGSEP HSALLIDDAA IGTDQDKKFV FVVDKDDHVG YREIQLGDLQ GSLRVVKGGL HAGDRIVVNG TQRVRPGAQI RAHMVPMDAG NGNSAPVADD AQADKQAQLD GSKQHAEPQK QAQAQPHAKT RK
Validated NT Sequence
ccttgcgtgt cttcgcgtgc ggctgcgcct gtgcctgctt ttgcggttcg gcgtgctgct tcgatccgtc gagttgcgcc tgcttgtccg cctgcgcgtc gtcggcgacg ggcgcgctgt tcccattgcc tgcgtccatc ggcaccatgt gcgcccggat ctgcgcaccg gggcgcactc gctgcgtgcc gttcacgacg atgcggtcgc ccgcgtgcag cccacccttg acgacgcgca gactgccctg cagatcgccc aactggattt cgcggtagcc gacgtgatcg tctttgtcga cgacgaacac gaacttcttg tcctggtccg tgccgatcgc cgcatcgtcg atcagcagcg ccgaatgcgg ctcgctgccg cccactttca cgcgtgcata gaggcctggg accagcgagc cgtcagcgtt gtcgaaacgt gcgcgcacgc ggatcgtgcc tgacgacgta tccagccggt tgtcgaccga ctggatcgtg cccttgcgcg aatagcccgt ttcgttcgcg aggcccagtt cgacgggcac cttgctgtcg ctcttcacct ggctgatgta ctgaagatac gtctgctcgt cggcgtcgaa ctccgcgtag atcggcgaca ccgacacgag cgtcgtcagc ggcgcggcgc tcgcgcccgc gttcacgaca ttgccgagcg tgatctcggc acgcgacacg cggcccgaca cgggcgcgac gatcttcgta tagccgaggt tgatctgcgc cgcttcgagc gccgcttgcg cggccttcac attcgcgttc gcttcgcgcg ccgcattctg cttctcgtcg taatcgcgcc tggcaatcgc gctatcgccg atcagccgtt gcgcacgctc ccagtcgctt tgcgcgtagc ctgcgcgcga ctgcgcagcg gcgagctgtg ccgcggcgcg atcgacttcc gcctgatacg ggcgcggatc gatcacgaag agcacgtcgc ccttcttcac gagcgcgccg tcctggaagt tcacggacac gatggtgcca gagacgagcg ggcgcacctc gaccttttcc actgcctgaa gacggcccga atagctttgc cagtcggtga tggttttctg aatgacggtc gcgacgtcga cttccggcac gatggtcggc gcggattgcc cggctgagcg tgcatccacg cggatcgcgc ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAIRVDAR SAGQSAPTIV PEVDVATVIQ KTITDWQSYS GRLQAVEKVE VRPLVSGTIV SVNFQDGALV KKGDVLFVID PRPYQAEVDR AAAQLAAAQS RAGYAQSDWE RAQRLIGDSA IARRDYDEKQ NAAREANANV KAAQAALEAA QINLGYTKIV APVSGRVSRA EITLGNVVNA GASAAPLTTL VSVSPIYAEF DADEQTYLQY ISQVKSDSKV PVELGLANET GYSRKGTIQS VDNRLDTSSG TIRVRARFDN ADGSLVPGLY ARVKVGGSEP HSALLIDDAA IGTDQDKKFV FVVDKDDHVG YREIQLGDLQ GSLRVVKGGL HAGDRIVVNG TQRVRPGAQI RAHMVPMDAG NGNSAPVADD AQADKQAQLD GSKQHAEPQK QAQAQPHAKT RKDS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGCGATC CGCGTGGATG CACGCTCAGC CGGGCAATCC GCGCCGACCA TCGTGCCGGA AGTCGACGTC GCGACCGTCA TTCAGAAAAC CATCACCGAC TGGCAAAGCT ATTCGGGCCG TCTTCAGGCA GTGGAAAAGG TCGAGGTGCG CCCGCTCGTC TCTGGCACCA TCGTGTCCGT GAACTTCCAG GACGGCGCGC TCGTGAAGAA GGGCGACGTG CTCTTCGTGA TCGATCCGCG CCCGTATCAG GCGGAAGTCG ATCGCGCCGC GGCACAGCTC GCCGCTGCGC AGTCGCGCGC AGGCTACGCG CAAAGCGACT GGGAGCGTGC GCAACGGCTG ATCGGCGATA GCGCGATTGC CAGGCGCGAT TACGACGAGA AGCAGAATGC GGCGCGCGAA GCGAACGCGA ATGTGAAGGC CGCGCAAGCG GCGCTCGAAG CGGCGCAGAT CAACCTCGGC TATACGAAGA TCGTCGCGCC CGTGTCGGGC CGCGTGTCGC GTGCCGAGAT CACGCTCGGC AATGTCGTGA ACGCGGGCGC GAGCGCCGCG CCGCTGACGA CGCTCGTGTC GGTGTCGCCG ATCTACGCGG AGTTCGACGC CGACGAGCAG ACGTATCTTC AGTACATCAG CCAGGTGAAG AGCGACAGCA AGGTGCCCGT CGAACTGGGC CTCGCGAACG AAACGGGCTA TTCGCGCAAG GGCACGATCC AGTCGGTCGA CAACCGGCTG GATACGTCGT CAGGCACGAT CCGCGTGCGC GCACGTTTCG ACAACGCTGA CGGCTCGCTG GTCCCAGGCC TCTATGCACG CGTGAAAGTG GGCGGCAGCG AGCCGCATTC GGCGCTGCTG ATCGACGATG CGGCGATCGG CACGGACCAG GACAAGAAGT TCGTGTTCGT CGTCGACAAA GACGATCACG TCGGCTACCG CGAAATCCAG TTGGGCGATC TGCAGGGCAG TCTGCGCGTC GTCAAGGGTG GGCTGCACGC GGGCGACCGC ATCGTCGTGA ACGGCACGCA GCGAGTGCGC CCCGGTGCGC AGATCCGGGC GCACATGGTG CCGATGGACG CAGGCAATGG GAACAGCGCG CCCGTCGCCG ACGACGCGCA GGCGGACAAG CAGGCGCAAC TCGACGGATC GAAGCAGCAC GCCGAACCGC AAAAGCAGGC ACAGGCGCAG CCGCACGCGA AGACACGCAA GGATTCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA