BuphA.00107.e

Efflux transporter, RND family, MFP subunit

CENTER ID: BuphA.00107.e
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00107.e.A1.GE34737 Full length( BuphA.00107.e ) 1 445
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00107.e.A1.PS01630 Full length( BuphA.00107.e ) 1 445

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.5790
RefSeq: YP_001861626.1
UniProt: B2JP27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASWQSNRAS DFGAGFGGSV GEDGPAQDGP AQKRPLASRF ALAAALVSAL SLTACKKAPT EPPRPTVEVT VVTVAQKETP VDFEFTAQTQ SSREVEIRAR VDGFLERRLY DEGALVKEGQ VLFVIDRKPF EAALQSARGV LAQQQARLLV TRQNLARVVP LAAQNALSKK DLDDATGNEK EAEAAVIAAQ GEVRTAELNL SYCTIRSPLK GLSSFARVQD GSYVTPTQSG LLTYVYQLDP MWVNFSISEN ELLRYREQIA KGLLRFPADN RFDVTVIQAD GSVYPQKGRI DFTNPAFSQQ TGTFLVRATF ANPDGTLRPG QFVRAKVSGA VRPNAILVPQ RAVLQGAKSH FVWVLDQDAK PHQRVVDVGD WHGDDWFVNE GLKPGERVVV DGAIRVSSDA QIKVVSAPPG SGAPGTPAAP ASAPAAAASV PAATVAGTQA RPASP
NT Sequence
ATGGCGTCTT GGCAGTCGAA TCGCGCATCT GATTTCGGCG CAGGCTTCGG TGGATCGGTT GGAGAAGACG GGCCGGCCCA AGACGGGCCG GCCCAAAAAC GGCCGCTGGC ATCACGCTTT GCGCTCGCGG CGGCATTGGT GTCCGCCTTG TCCCTGACCG CCTGTAAAAA AGCGCCCACC GAACCCCCGC GCCCGACTGT CGAAGTGACG GTGGTGACGG TTGCGCAGAA GGAAACGCCC GTCGACTTCG AGTTCACCGC GCAGACGCAG AGTTCGCGCG AAGTCGAAAT CCGCGCGCGC GTCGACGGCT TTCTCGAGCG GCGTCTGTAC GACGAAGGCG CGCTGGTCAA GGAAGGCCAG GTGCTGTTCG TGATAGACCG CAAGCCCTTC GAAGCCGCGC TGCAATCGGC CAGGGGCGTG CTCGCGCAGC AGCAGGCGCG CCTGCTCGTC ACCAGACAGA ACCTCGCGCG CGTCGTGCCG CTCGCCGCGC AGAACGCGCT GAGCAAGAAG GACCTGGACG ACGCCACCGG CAACGAGAAG GAAGCCGAAG CCGCCGTGAT CGCCGCGCAG GGCGAGGTGC GCACGGCCGA ACTGAACCTG AGCTACTGCA CGATCCGCTC GCCGCTCAAG GGGCTGTCGA GCTTCGCGCG CGTGCAGGAC GGCAGCTACG TCACGCCGAC GCAATCGGGC CTGCTGACGT ACGTCTACCA GCTCGATCCG ATGTGGGTGA ACTTCAGCAT CTCCGAGAAC GAACTGCTGC GCTACCGCGA GCAGATCGCC AAGGGCCTGC TGCGCTTTCC CGCCGACAAC CGGTTCGACG TGACCGTGAT CCAGGCCGAC GGCTCGGTCT ATCCGCAAAA AGGCCGTATC GATTTCACGA ACCCCGCGTT CAGCCAGCAG ACGGGCACCT TCCTCGTGCG CGCGACCTTC GCCAATCCGG ACGGCACGCT GCGCCCGGGC CAGTTCGTGC GCGCGAAAGT GTCGGGCGCG GTGCGCCCCA ACGCGATCCT CGTGCCGCAG CGCGCGGTGC TGCAGGGCGC GAAGAGCCAC TTCGTGTGGG TGCTCGACCA GGACGCGAAG CCGCATCAGC GTGTGGTCGA TGTCGGCGAC TGGCACGGCG ACGACTGGTT CGTCAACGAA GGCCTGAAGC CGGGCGAGCG CGTGGTGGTC GACGGCGCGA TCCGCGTGTC GTCGGATGCG CAGATCAAGG TGGTGAGCGC GCCGCCGGGC TCGGGCGCGC CCGGCACGCC CGCGGCCCCT GCGTCGGCGC CCGCGGCGGC GGCGTCCGTG CCCGCGGCGA CCGTGGCCGG GACGCAGGCG CGTCCGGCCA GCCCC
Details for BuphA.00107.e.A1.GE34737
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 49kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 14
PERCENT COVERAGE: 31
Validated AA Sequence
MLLVVLFKKT FFLXLAEHLE VILCSGNSLI KKLHTIFTRT LNDGLSFLLG LXQLLNQRVG TLASTHRTRT LGLXFQGTHM MVXVMSHXXX XXXXXXXXXX XXNRCGLPIX XXXXXXSAGX XXXXXXXXXX XRXXSXSXML KFTHIGSSW
Validated NT Sequence
ttatgtcaat gtcaagggtg gcagctacaa gcttgttgac nagcatgtcc ttgcactccc taaaacgctc atcatactgc ctaatctttt cctttgcctt cttgtcnnan tcgtccaatt gaggttcatc atcgccatgt tgctggtggt actcttcaag aagacgtttt tcctcantct cgctgaacac cttgaggtca ttctctgcag cggaaatagc ctcattaaga agcttcacac gattttcacg cgcacgctta acgatggcct cagcttcctc ctcggccttc ancaattgct gaatcaacgc gttggaaccc ttgccagtac tcatcgaacc aggaccctgg gtctganctt ccagggtacc catatgatgg tgntggtgat gagccatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaccgttg tggtctccct atannnnnnn nnatnannnn nnngtcggcg ggnnnnnnna nnnngnncnt gncgancnnn nnnnngtanc gcancanttc gntctcnnan atgctgaagt tcacccacat cggatcgagc tggtagacgt acgtcagcag gccngattgc gtnggcgtga cgtagctgcc gtcctgcacg cgcgcgaagc tcgacagccc cttgagcggc gagcggatcg tncagtagnt caggttcagn tcggccgtgc gcacctcgcc ntgcgcggcg atcacggcgg cttcggcttc cttctcgttg ccggtgnnnt ngtccagntc cttcttgctc agcgcgttct gcgcggcgag cggcacgacg cgcgcgaggt tctgtctggt gacgagcagg cgcgcctgct gctgcgcgag cacgcccctg gccgattgca gcgcggcttc gaagggcttg cggtctatca cgaacagcac ctggccttcc ttgaccagcg cgccttcgtc gtacagacgc cgctcgagaa agccgtcgac gcgcgcgcgg atttcgactt cgcgcgaact ctgcgt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASWQSNRA SDFGAGFGGS VGEDGPAQDG PAQKRPLASR FALAAALVSA LSLTACKKAP TEPPRPTVEV TVVTVAQKET PVDFEFTAQT QSSREVEIRA RVDGFLERRL YDEGALVKEG QVLFVIDRKP FEAALQSARG VLAQQQARLL VTRQNLARVV PLAAQNALSK KDLDDATGNE KEAEAAVIAA QGEVRTAELN LSYCTIRSPL KGLSSFARVQ DGSYVTPTQS GLLTYVYQLD PMWVNFSISE NELLRYREQI AKGLLRFPAD NRFDVTVIQA DGSVYPQKGR IDFTNPAFSQ QTGTFLVRAT FANPDGTLRP GQFVRAKVSG AVRPNAILVP QRAVLQGAKS HFVWVLDQDA KPHQRVVDVG DWHGDDWFVN EGLKPGERVV VDGAIRVSSD AQIKVVSAPP GSGAPGTPAA PASAPAAAAS VPAATVAGTQ ARPASP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtcttgg cagtcgaatc gcgcatctga tttcggcgca ggcttcggtg gatcggttgg agaagacggg ccggcccaag acgggccggc ccaaaaacgg ccgctggcat cacgctttgc gctcgcggcg gcattggtgt ccgccttgtc cctgaccgcc tgtaaaaaag cgcccaccga acccccgcgc ccgactgtcg aagtgacggt ggtgacggtt gcgcagaagg aaacgcccgt cgacttcgag ttcaccgcgc agacgcagag ttcgcgcgaa gtcgaaatcc gcgcgcgcgt cgacggcttt ctcgagcggc gtctgtacga cgaaggcgcg ctggtcaagg aaggccaggt gctgttcgtg atagaccgca agcccttcga agccgcgctg caatcggcca ggggcgtgct cgcgcagcag caggcgcgcc tgctcgtcac cagacagaac ctcgcgcgcg tcgtgccgct cgccgcgcag aacgcgctga gcaagaagga cctggacgac gccaccggca acgagaagga agccgaagcc gccgtgatcg ccgcgcaggg cgaggtgcgc acggccgaac tgaacctgag ctactgcacg atccgctcgc cgctcaaggg gctgtcgagc ttcgcgcgcg tgcaggacgg cagctacgtc acgccgacgc aatcgggcct gctgacgtac gtctaccagc tcgatccgat gtgggtgaac ttcagcatct ccgagaacga actgctgcgc taccgcgagc agatcgccaa gggcctgctg cgctttcccg ccgacaaccg gttcgacgtg accgtgatcc aggccgacgg ctcggtctat ccgcaaaaag gccgtatcga tttcacgaac cccgcgttca gccagcagac gggcaccttc ctcgtgcgcg cgaccttcgc caatccggac ggcacgctgc gcccgggcca gttcgtgcgc gcgaaagtgt cgggcgcggt gcgccccaac gcgatcctcg tgccgcagcg cgcggtgctg cagggcgcga agagccactt cgtgtgggtg ctcgaccagg acgcgaagcc gcatcagcgt gtggtcgatg tcggcgactg gcacggcgac gactggttcg tcaacgaagg cctgaagccg ggcgagcgcg tggtggtcga cggcgcgatc cgcgtgtcgt cggatgcgca gatcaaggtg gtgagcgcgc cgccgggctc gggcgcgccc ggcacgcccg cggcccctgc gtcggcgccc gcggcggcgg cgtccgtgcc cgcggcgacc gtggccggga cgcaggcgcg tccggccagc ccctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BuphA.00107.e.A1.PS01630
PURIFICATION DATe: 9/5/2012
CONCENTRATION: 26.15mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 14
PERCENT COVERAGE: 31
Protocol Notes
notes unavailable
Validated AA Sequence
MLLVVLFKKT FFLXLAEHLE VILCSGNSLI KKLHTIFTRT LNDGLSFLLG LXQLLNQRVG TLASTHRTRT LGLXFQGTHM MVXVMSHXXX XXXXXXXXXX XXNRCGLPIX XXXXXXSAGX XXXXXXXXXX XRXXSXSXML KFTHIGSSW
Validated NT Sequence
ttatgtcaat gtcaagggtg gcagctacaa gcttgttgac nagcatgtcc ttgcactccc taaaacgctc atcatactgc ctaatctttt cctttgcctt cttgtcnnan tcgtccaatt gaggttcatc atcgccatgt tgctggtggt actcttcaag aagacgtttt tcctcantct cgctgaacac cttgaggtca ttctctgcag cggaaatagc ctcattaaga agcttcacac gattttcacg cgcacgctta acgatggcct cagcttcctc ctcggccttc ancaattgct gaatcaacgc gttggaaccc ttgccagtac tcatcgaacc aggaccctgg gtctganctt ccagggtacc catatgatgg tgntggtgat gagccatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaccgttg tggtctccct atannnnnnn nnatnannnn nnngtcggcg ggnnnnnnna nnnngnncnt gncgancnnn nnnnngtanc gcancanttc gntctcnnan atgctgaagt tcacccacat cggatcgagc tggtagacgt acgtcagcag gccngattgc gtnggcgtga cgtagctgcc gtcctgcacg cgcgcgaagc tcgacagccc cttgagcggc gagcggatcg tncagtagnt caggttcagn tcggccgtgc gcacctcgcc ntgcgcggcg atcacggcgg cttcggcttc cttctcgttg ccggtgnnnt ngtccagntc cttcttgctc agcgcgttct gcgcggcgag cggcacgacg cgcgcgaggt tctgtctggt gacgagcagg cgcgcctgct gctgcgcgag cacgcccctg gccgattgca gcgcggcttc gaagggcttg cggtctatca cgaacagcac ctggccttcc ttgaccagcg cgccttcgtc gtacagacgc cgctcgagaa agccgtcgac gcgcgcgcgg atttcgactt cgcgcgaact ctgcgt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASWQSNRA SDFGAGFGGS VGEDGPAQDG PAQKRPLASR FALAAALVSA LSLTACKKAP TEPPRPTVEV TVVTVAQKET PVDFEFTAQT QSSREVEIRA RVDGFLERRL YDEGALVKEG QVLFVIDRKP FEAALQSARG VLAQQQARLL VTRQNLARVV PLAAQNALSK KDLDDATGNE KEAEAAVIAA QGEVRTAELN LSYCTIRSPL KGLSSFARVQ DGSYVTPTQS GLLTYVYQLD PMWVNFSISE NELLRYREQI AKGLLRFPAD NRFDVTVIQA DGSVYPQKGR IDFTNPAFSQ QTGTFLVRAT FANPDGTLRP GQFVRAKVSG AVRPNAILVP QRAVLQGAKS HFVWVLDQDA KPHQRVVDVG DWHGDDWFVN EGLKPGERVV VDGAIRVSSD AQIKVVSAPP GSGAPGTPAA PASAPAAAAS VPAATVAGTQ ARPASP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGTCTTGG CAGTCGAATC GCGCATCTGA TTTCGGCGCA GGCTTCGGTG GATCGGTTGG AGAAGACGGG CCGGCCCAAG ACGGGCCGGC CCAAAAACGG CCGCTGGCAT CACGCTTTGC GCTCGCGGCG GCATTGGTGT CCGCCTTGTC CCTGACCGCC TGTAAAAAAG CGCCCACCGA ACCCCCGCGC CCGACTGTCG AAGTGACGGT GGTGACGGTT GCGCAGAAGG AAACGCCCGT CGACTTCGAG TTCACCGCGC AGACGCAGAG TTCGCGCGAA GTCGAAATCC GCGCGCGCGT CGACGGCTTT CTCGAGCGGC GTCTGTACGA CGAAGGCGCG CTGGTCAAGG AAGGCCAGGT GCTGTTCGTG ATAGACCGCA AGCCCTTCGA AGCCGCGCTG CAATCGGCCA GGGGCGTGCT CGCGCAGCAG CAGGCGCGCC TGCTCGTCAC CAGACAGAAC CTCGCGCGCG TCGTGCCGCT CGCCGCGCAG AACGCGCTGA GCAAGAAGGA CCTGGACGAC GCCACCGGCA ACGAGAAGGA AGCCGAAGCC GCCGTGATCG CCGCGCAGGG CGAGGTGCGC ACGGCCGAAC TGAACCTGAG CTACTGCACG ATCCGCTCGC CGCTCAAGGG GCTGTCGAGC TTCGCGCGCG TGCAGGACGG CAGCTACGTC ACGCCGACGC AATCGGGCCT GCTGACGTAC GTCTACCAGC TCGATCCGAT GTGGGTGAAC TTCAGCATCT CCGAGAACGA ACTGCTGCGC TACCGCGAGC AGATCGCCAA GGGCCTGCTG CGCTTTCCCG CCGACAACCG GTTCGACGTG ACCGTGATCC AGGCCGACGG CTCGGTCTAT CCGCAAAAAG GCCGTATCGA TTTCACGAAC CCCGCGTTCA GCCAGCAGAC GGGCACCTTC CTCGTGCGCG CGACCTTCGC CAATCCGGAC GGCACGCTGC GCCCGGGCCA GTTCGTGCGC GCGAAAGTGT CGGGCGCGGT GCGCCCCAAC GCGATCCTCG TGCCGCAGCG CGCGGTGCTG CAGGGCGCGA AGAGCCACTT CGTGTGGGTG CTCGACCAGG ACGCGAAGCC GCATCAGCGT GTGGTCGATG TCGGCGACTG GCACGGCGAC GACTGGTTCG TCAACGAAGG CCTGAAGCCG GGCGAGCGCG TGGTGGTCGA CGGCGCGATC CGCGTGTCGT CGGATGCGCA GATCAAGGTG GTGAGCGCGC CGCCGGGCTC GGGCGCGCCC GGCACGCCCG CGGCCCCTGC GTCGGCGCCC GCGGCGGCGG CGTCCGTGCC CGCGGCGACC GTGGCCGGGA CGCAGGCGCG TCCGGCCAGC CCCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA