BuphA.00107.d

Efflux transporter, RND family, MFP subunit

CENTER ID: BuphA.00107.d
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00107.d.A2.GE32274 Mature protein( BuphA.00107.d ) 27 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00107.d.A2.PS01242 Mature protein( BuphA.00107.d ) 27 405

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.3689
RefSeq: YP_001859687.1
UniProt: B2JLK8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPALLKNRNF RMLAATAAIS AVLPLLAACG DQHAAPPQQT PEVGVVTIRP SAVPVTVELP GRVSAHLVAE VRARVDGIVQ KRLYTEGSEV KAGQTLYKID PAPYIASLNS AKATLAKAQA TLVSAHAEAA RYKVLVAANA ISKQDYDNAV ASEGQATADV AAGKAAVDTA QINLGYTDVS SPISGRIGKS SVTEGAYVQS SAATLLATVQ QLDPVYVDLT QSSLVGLKLR RDAQEGRLKT SGPNAAKVTL ILEDGRPYGG TGKLEFSDVT VDQTTGSVTV RALFDNAQGV LLPGMYVRAK IEEGVNEQAF VVPQQGVTHD QKGDPTALVV GADGKVAMRQ LVTSGTYGAN WVVESGLQTG DRVIVQGVDK VRPGMSVKTV AAQLPAADTA TQTAATPASG AQSAQ
NT Sequence
ATGCCTGCGC TATTAAAGAA CCGTAACTTT CGAATGCTCG CGGCCACGGC TGCGATTTCC GCCGTGCTAC CGCTACTCGC CGCGTGCGGC GACCAGCACG CAGCCCCGCC GCAACAGACG CCCGAAGTCG GCGTGGTTAC CATCCGGCCT TCCGCCGTGC CCGTCACCGT CGAATTGCCA GGCCGCGTGA GCGCGCATCT GGTCGCCGAA GTGCGCGCGC GCGTCGACGG CATCGTGCAG AAGCGCCTCT ATACAGAGGG CAGCGAAGTG AAGGCGGGGC AAACGCTCTA CAAGATCGAC CCCGCGCCGT ACATCGCATC GCTGAACAGC GCGAAGGCGA CGCTTGCGAA GGCGCAGGCG ACGCTGGTCT CCGCACATGC CGAGGCCGCG CGCTATAAGG TGCTGGTCGC AGCGAACGCG ATCAGCAAGC AGGACTACGA CAACGCCGTA GCATCCGAAG GCCAGGCCAC CGCCGATGTC GCAGCAGGCA AGGCAGCCGT CGACACCGCG CAGATCAACC TCGGTTATAC CGACGTGAGC TCGCCGATCT CGGGACGCAT CGGCAAGTCG TCGGTGACGG AAGGCGCATA CGTGCAGTCC AGCGCGGCCA CGCTGCTCGC AACCGTGCAG CAGCTCGATC CCGTCTACGT GGATCTCACG CAATCGAGCC TCGTCGGCCT CAAGCTGCGC CGCGACGCAC AGGAAGGGCG CCTCAAGACG AGCGGACCAA ACGCGGCGAA AGTCACGTTG ATTCTCGAAG ACGGCCGTCC TTACGGCGGC ACGGGCAAGC TCGAATTCTC CGACGTGACC GTCGATCAGA CGACGGGCTC CGTCACTGTG CGCGCGCTGT TCGATAACGC GCAGGGTGTG CTGCTGCCGG GCATGTATGT GCGCGCGAAG ATTGAAGAGG GCGTGAACGA GCAGGCGTTC GTCGTGCCGC AACAGGGCGT CACGCATGAC CAGAAGGGCG ATCCGACCGC GCTCGTGGTC GGCGCCGACG GCAAGGTTGC GATGCGGCAA CTGGTGACGT CGGGCACCTA CGGCGCAAAT TGGGTTGTCG AGAGCGGGCT GCAAACGGGC GACCGCGTGA TCGTGCAAGG CGTCGACAAG GTGCGCCCGG GCATGAGCGT GAAGACAGTC GCGGCACAGC TGCCCGCCGC CGATACGGCC ACGCAGACGG CGGCAACGCC CGCGTCGGGC GCGCAGAGCG CGCAG
Details for BuphA.00107.d.A2.GE32274
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MAACGDQHAA PPQQTPEVGV VTIRPSAVPV TVELPGRVSA HLVAEVRARV DGIVQKRLYT EGSEVKAGQT LYKIDPAPYI ASLNSAKATL AKAQATLVSA HAEAARYKVL VAANAISKQD YDNAVASEGQ ATADVAAGKA AVDTAQINLG YTDVSSPISG RIGKSSVTEG AYVQSSAATL LATVQQLDPV YVDLTQSSLV GLKLRRDAQE GRLKTSGPNA AKVTLILEDG RPYGGTGKLE FSDVTVDQTT GSVTVRALFD NAQGVLLPGM YVRAKIEEGV NEQAFVVPQQ GVTHDQKGDP TALVVGADGK VAMRQLVTSG TYGANWVVES GLQTGDRVIV QGVDKVRPGM SVKTVAAQLP AADTATQTAA TPASGAQSAQ
Validated NT Sequence
ttattactgc gcgctctgcg cgcccgacgc gggcgttgcc gccgtctgcg tggccgtatc ggcggcgggc agctgtgccg cgactgtctt cacgctcatg cccgggcgca ccttgtcgac gccttgcacg atcacgcggt cgcccgtttg cagcccgctc tcgacaaccc aatttgcgcc gtaggtgccc gacgtcacca gttgccgcat cgcaaccttg ccgtcggcgc cgaccacgag cgcggtcgga tcgcccttct ggtcatgcgt gacgccctgt tgcggcacga cgaacgcctg ctcgttcacg ccctcttcaa tcttcgcgcg cacatacatg cccggcagca gcacaccctg cgcgttatcg aacagcgcgc gcacagtgac ggagcccgtc gtctgatcga cggtcacgtc ggagaattcg agcttgcccg tgccgccgta aggacggccg tcttcgagaa tcaacgtgac tttcgccgcg tttggtccgc tcgtcttgag gcgcccttcc tgtgcgtcgc ggcgcagctt gaggccgacg aggctcgatt gcgtgagatc cacgtagacg ggatcgagct gctgcacggt tgcgagcagc gtggccgcgc tggactgcac gtatgcgcct tccgtcaccg acgacttgcc gatgcgtccc gagatcggcg agctcacgtc ggtataaccg aggttgatct gcgcggtgtc gacggctgcc ttgcctgctg cgacatcggc ggtggcctgg ccttcggatg ctacggcgtt gtcgtagtcc tgcttgctga tcgcgttcgc tgcgaccagc accttatagc gcgcggcctc ggcatgtgcg gagaccagcg tcgcctgcgc cttcgcaagc gtcgccttcg cgctgttcag cgatgcgatg tacggcgcgg ggtcgatctt gtagagcgtt tgccccgcct tcacttcgct gccctctgta tagaggcgct tctgcacgat gccgtcgacg cgcgcgcgca cttcggcgac cagatgcgcg ctcacgcggc ctggcaattc gacggtgacg ggcacggcgg aaggccggat ggtaaccacg ccgacttcgg gcgtctgttg cggcggggct gcgtgctggt cgccgcacgc ggccatcgaa ccaggaccct gggtctgagc ttccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAACGDQHA APPQQTPEVG VVTIRPSAVP VTVELPGRVS AHLVAEVRAR VDGIVQKRLY TEGSEVKAGQ TLYKIDPAPY IASLNSAKAT LAKAQATLVS AHAEAARYKV LVAANAISKQ DYDNAVASEG QATADVAAGK AAVDTAQINL GYTDVSSPIS GRIGKSSVTE GAYVQSSAAT LLATVQQLDP VYVDLTQSSL VGLKLRRDAQ EGRLKTSGPN AAKVTLILED GRPYGGTGKL EFSDVTVDQT TGSVTVRALF DNAQGVLLPG MYVRAKIEEG VNEQAFVVPQ QGVTHDQKGD PTALVVGADG KVAMRQLVTS GTYGANWVVE SGLQTGDRVI VQGVDKVRPG MSVKTVAAQL PAADTATQTA ATPASGAQSA Q
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgcgtgc ggcgaccagc acgcagcccc gccgcaacag acgcccgaag tcggcgtggt taccatccgg ccttccgccg tgcccgtcac cgtcgaattg ccaggccgcg tgagcgcgca tctggtcgcc gaagtgcgcg cgcgcgtcga cggcatcgtg cagaagcgcc tctatacaga gggcagcgaa gtgaaggcgg ggcaaacgct ctacaagatc gaccccgcgc cgtacatcgc atcgctgaac agcgcgaagg cgacgcttgc gaaggcgcag gcgacgctgg tctccgcaca tgccgaggcc gcgcgctata aggtgctggt cgcagcgaac gcgatcagca agcaggacta cgacaacgcc gtagcatccg aaggccaggc caccgccgat gtcgcagcag gcaaggcagc cgtcgacacc gcgcagatca acctcggtta taccgacgtg agctcgccga tctcgggacg catcggcaag tcgtcggtga cggaaggcgc atacgtgcag tccagcgcgg ccacgctgct cgcaaccgtg cagcagctcg atcccgtcta cgtggatctc acgcaatcga gcctcgtcgg cctcaagctg cgccgcgacg cacaggaagg gcgcctcaag acgagcggac caaacgcggc gaaagtcacg ttgattctcg aagacggccg tccttacggc ggcacgggca agctcgaatt ctccgacgtg accgtcgatc agacgacggg ctccgtcact gtgcgcgcgc tgttcgataa cgcgcagggt gtgctgctgc cgggcatgta tgtgcgcgcg aagattgaag agggcgtgaa cgagcaggcg ttcgtcgtgc cgcaacaggg cgtcacgcat gaccagaagg gcgatccgac cgcgctcgtg gtcggcgccg acggcaaggt tgcgatgcgg caactggtga cgtcgggcac ctacggcgca aattgggttg tcgagagcgg gctgcaaacg ggcgaccgcg tgatcgtgca aggcgtcgac aaggtgcgcc cgggcatgag cgtgaagaca gtcgcggcac agctgcccgc cgccgatacg gccacgcaga cggcggcaac gcccgcgtcg ggcgcgcaga gcgcgcagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuphA.00107.d.A2.PS01242
PURIFICATION DATe: 10/20/2011
CONCENTRATION: 50.08mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MAACGDQHAA PPQQTPEVGV VTIRPSAVPV TVELPGRVSA HLVAEVRARV DGIVQKRLYT EGSEVKAGQT LYKIDPAPYI ASLNSAKATL AKAQATLVSA HAEAARYKVL VAANAISKQD YDNAVASEGQ ATADVAAGKA AVDTAQINLG YTDVSSPISG RIGKSSVTEG AYVQSSAATL LATVQQLDPV YVDLTQSSLV GLKLRRDAQE GRLKTSGPNA AKVTLILEDG RPYGGTGKLE FSDVTVDQTT GSVTVRALFD NAQGVLLPGM YVRAKIEEGV NEQAFVVPQQ GVTHDQKGDP TALVVGADGK VAMRQLVTSG TYGANWVVES GLQTGDRVIV QGVDKVRPGM SVKTVAAQLP AADTATQTAA TPASGAQSAQ
Validated NT Sequence
ttattactgc gcgctctgcg cgcccgacgc gggcgttgcc gccgtctgcg tggccgtatc ggcggcgggc agctgtgccg cgactgtctt cacgctcatg cccgggcgca ccttgtcgac gccttgcacg atcacgcggt cgcccgtttg cagcccgctc tcgacaaccc aatttgcgcc gtaggtgccc gacgtcacca gttgccgcat cgcaaccttg ccgtcggcgc cgaccacgag cgcggtcgga tcgcccttct ggtcatgcgt gacgccctgt tgcggcacga cgaacgcctg ctcgttcacg ccctcttcaa tcttcgcgcg cacatacatg cccggcagca gcacaccctg cgcgttatcg aacagcgcgc gcacagtgac ggagcccgtc gtctgatcga cggtcacgtc ggagaattcg agcttgcccg tgccgccgta aggacggccg tcttcgagaa tcaacgtgac tttcgccgcg tttggtccgc tcgtcttgag gcgcccttcc tgtgcgtcgc ggcgcagctt gaggccgacg aggctcgatt gcgtgagatc cacgtagacg ggatcgagct gctgcacggt tgcgagcagc gtggccgcgc tggactgcac gtatgcgcct tccgtcaccg acgacttgcc gatgcgtccc gagatcggcg agctcacgtc ggtataaccg aggttgatct gcgcggtgtc gacggctgcc ttgcctgctg cgacatcggc ggtggcctgg ccttcggatg ctacggcgtt gtcgtagtcc tgcttgctga tcgcgttcgc tgcgaccagc accttatagc gcgcggcctc ggcatgtgcg gagaccagcg tcgcctgcgc cttcgcaagc gtcgccttcg cgctgttcag cgatgcgatg tacggcgcgg ggtcgatctt gtagagcgtt tgccccgcct tcacttcgct gccctctgta tagaggcgct tctgcacgat gccgtcgacg cgcgcgcgca cttcggcgac cagatgcgcg ctcacgcggc ctggcaattc gacggtgacg ggcacggcgg aaggccggat ggtaaccacg ccgacttcgg gcgtctgttg cggcggggct gcgtgctggt cgccgcacgc ggccatcgaa ccaggaccct gggtctgagc ttccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAACGDQHA APPQQTPEVG VVTIRPSAVP VTVELPGRVS AHLVAEVRAR VDGIVQKRLY TEGSEVKAGQ TLYKIDPAPY IASLNSAKAT LAKAQATLVS AHAEAARYKV LVAANAISKQ DYDNAVASEG QATADVAAGK AAVDTAQINL GYTDVSSPIS GRIGKSSVTE GAYVQSSAAT LLATVQQLDP VYVDLTQSSL VGLKLRRDAQ EGRLKTSGPN AAKVTLILED GRPYGGTGKL EFSDVTVDQT TGSVTVRALF DNAQGVLLPG MYVRAKIEEG VNEQAFVVPQ QGVTHDQKGD PTALVVGADG KVAMRQLVTS GTYGANWVVE SGLQTGDRVI VQGVDKVRPG MSVKTVAAQL PAADTATQTA ATPASGAQSA Q
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGCGTGC GGCGACCAGC ACGCAGCCCC GCCGCAACAG ACGCCCGAAG TCGGCGTGGT TACCATCCGG CCTTCCGCCG TGCCCGTCAC CGTCGAATTG CCAGGCCGCG TGAGCGCGCA TCTGGTCGCC GAAGTGCGCG CGCGCGTCGA CGGCATCGTG CAGAAGCGCC TCTATACAGA GGGCAGCGAA GTGAAGGCGG GGCAAACGCT CTACAAGATC GACCCCGCGC CGTACATCGC ATCGCTGAAC AGCGCGAAGG CGACGCTTGC GAAGGCGCAG GCGACGCTGG TCTCCGCACA TGCCGAGGCC GCGCGCTATA AGGTGCTGGT CGCAGCGAAC GCGATCAGCA AGCAGGACTA CGACAACGCC GTAGCATCCG AAGGCCAGGC CACCGCCGAT GTCGCAGCAG GCAAGGCAGC CGTCGACACC GCGCAGATCA ACCTCGGTTA TACCGACGTG AGCTCGCCGA TCTCGGGACG CATCGGCAAG TCGTCGGTGA CGGAAGGCGC ATACGTGCAG TCCAGCGCGG CCACGCTGCT CGCAACCGTG CAGCAGCTCG ATCCCGTCTA CGTGGATCTC ACGCAATCGA GCCTCGTCGG CCTCAAGCTG CGCCGCGACG CACAGGAAGG GCGCCTCAAG ACGAGCGGAC CAAACGCGGC GAAAGTCACG TTGATTCTCG AAGACGGCCG TCCTTACGGC GGCACGGGCA AGCTCGAATT CTCCGACGTG ACCGTCGATC AGACGACGGG CTCCGTCACT GTGCGCGCGC TGTTCGATAA CGCGCAGGGT GTGCTGCTGC CGGGCATGTA TGTGCGCGCG AAGATTGAAG AGGGCGTGAA CGAGCAGGCG TTCGTCGTGC CGCAACAGGG CGTCACGCAT GACCAGAAGG GCGATCCGAC CGCGCTCGTG GTCGGCGCCG ACGGCAAGGT TGCGATGCGG CAACTGGTGA CGTCGGGCAC CTACGGCGCA AATTGGGTTG TCGAGAGCGG GCTGCAAACG GGCGACCGCG TGATCGTGCA AGGCGTCGAC AAGGTGCGCC CGGGCATGAG CGTGAAGACA GTCGCGGCAC AGCTGCCCGC CGCCGATACG GCCACGCAGA CGGCGGCAAC GCCCGCGTCG GGCGCGCAGA GCGCGCAGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A