BuphA.00104.a
Beta-lactamase
| CENTER ID: | BuphA.00104.a |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00104.a.B1.GE40114 | Full length( BuphA.00104.a ) | 1 | 281 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00104.a.B1.PS37967 | Full length( BuphA.00104.a ) | 1 | 281 |
Structures
5VPQ
DEPOSITED: 5/5/2017
DETERMINATION: XRay
CLONE: BuphA.00104.a.B1.GE40114
PROTEIN: BuphA.00104.a.B1.PS37967
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_143180 |
| PATRIC ID: | fig|391038.7.peg.2371 |
| RefSeq: | YP_001858450.1 |
| UniProt: | B2JET9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIGVRAQPAS SVSINLAARE RFEQLEASSG GRLGVAALRT ADGSYVGYRE SERFPMCSTF KLLAVALILK RSMAERGLLE ERIRYGDAQV VAHSPVTKRH AGGEGMTVGE LSAAALQHSD NTAANLLLTS LGGPEVLNQF ALSIGDTWFD LVRNEPEVNA SVPGDMRDTT SPRAMLLDVQ KLLLADDVLG AREREQLKAW MLGNTTGATR IRAAVPAAGW LVADNTGSGD YGTANDVAVV YPPSSAPLVV AIYFTGVTPK ETPPQDAIVD EAARIVFDAL R
NT Sequence
ATGATCGGCG TACGTGCGCA GCCGGCTTCC TCCGTATCGA TCAATCTTGC TGCGCGTGAG CGCTTCGAAC AACTGGAGGC ATCGAGTGGC GGCCGGCTCG GCGTCGCGGC GCTCAGAACG GCTGACGGTT CCTATGTCGG CTACCGGGAG TCGGAACGCT TTCCGATGTG CAGTACTTTC AAGCTGCTCG CCGTCGCATT GATCCTGAAG CGCAGCATGG CCGAACGCGG CTTGCTCGAA GAGCGCATCC GCTACGGCGA CGCTCAGGTC GTCGCTCATT CGCCCGTCAC GAAGCGGCAC GCAGGCGGCG AGGGAATGAC GGTAGGGGAG TTGAGTGCAG CGGCGCTCCA GCATAGCGAC AACACCGCGG CCAATCTGTT GCTGACGTCG CTTGGCGGGC CGGAGGTGCT GAACCAGTTT GCGTTATCGA TCGGCGACAC CTGGTTCGAC CTCGTTCGCA ACGAACCCGA AGTGAACGCA TCGGTGCCCG GGGACATGCG TGACACCACG TCGCCGCGCG CGATGCTGCT CGATGTGCAG AAACTGCTGC TCGCAGACGA CGTGCTGGGT GCGCGCGAGC GCGAGCAGTT GAAGGCGTGG ATGCTGGGCA ATACGACGGG TGCCACGCGC ATCCGCGCGG CCGTGCCTGC GGCCGGATGG TTGGTCGCGG ACAACACGGG CAGTGGAGAC TACGGCACGG CTAACGACGT CGCCGTGGTA TATCCGCCCT CGTCGGCGCC GTTGGTCGTC GCGATCTACT TCACGGGCGT GACGCCGAAG GAAACGCCGC CACAGGACGC AATCGTCGAC GAGGCGGCGC GAATCGTATT CGACGCGCTG CGA
Details for BuphA.00104.a.B1.GE40114
| HARVESTED ON: | 4/6/2016 |
| SEQUENCED ON: | 4/13/2016 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHMIG VRAQPASSVS INXAARERFE QLEASSGGRL GVAALRTADG SYVGYRESER FPMCSTFKLL AVALILKRSM AERGLLEERI RYGDAQVVAH SPVTKRHAGG EGMTVGELSA AALQHSDNTA ANLLLTSLGG PEVLNQFALS IGDTWFDLVR NEPEVNASVP GDMRDTTSPR AMLLDVQKLL LADDVLGARE REQLKAWMLG NTTGATRIRA AVPAAGWLVA DNTGSGDYGT ANDVAVVYPP SSAPLVVAIY FTGVTPKETP PQDAIVDEAA XXVFDALR
Validated NT Sequence
atcttactca cttatcgcag cgcgtcgaat acgnnnnnng ccgcctcgtc gacgattgcg tcctgtggcg gcgtttcctt cggcgtcacg cccgtgaagt agatcgcgac gaccaacggc gccgacgagg gcggatatac cacggcgacg tcgttagccg tgccgtagtc tccactgccc gtgttgtccg cgaccaacca tccggccgca ggcacggccg cgcggatgcg cgtggcaccc gtcgtattgc ccagcatcca cgccttcaac tgctcgcgct cgcgcgcacc cagcacgtcg tctgcgagca gcagtttctg cacatcgagc agcatcgcgc gcggcgacgt ggtgtcacgc atgtccccgg gcaccgatgc gttcacttcg ggttcgttgc gaacgaggtc gaaccaggtg tcgccgatcg ataacgcaaa ctggttcagc acctccggcc cgccaagcga cgtcagcaac agattggccg cggtgttgtc gctatgctgg agcgccgctg cactcaactc ccctaccgtc attccctcgc cgcctgcgtg ccgcttcgtg acgggcgaat gagcgacgac ctgagcgtcg ccgtagcgga tgcgctcttc gagcaagccg cgttcggcca tgctgcgctt caggatcaat gcgacggcga gcagcttgaa agtactgcac atcggaaagc gttccgactc ccggtagccg acataggaac cgtcagccgt tctgagcgcc gcgacgccga gccggccgcc actcgatgcc tccagttgtt cgaagcgctc acgcgcagca anattgatcg atacggagga agccggctgc gcacgtacgc cgatcatatg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMI GVRAQPASSV SINLAARERF EQLEASSGGR LGVAALRTAD GSYVGYRESE RFPMCSTFKL LAVALILKRS MAERGLLEER IRYGDAQVVA HSPVTKRHAG GEGMTVGELS AAALQHSDNT AANLLLTSLG GPEVLNQFAL SIGDTWFDLV RNEPEVNASV PGDMRDTTSP RAMLLDVQKL LLADDVLGAR EREQLKAWML GNTTGATRIR AAVPAAGWLV ADNTGSGDYG TANDVAVVYP PSSAPLVVAI YFTGVTPKET PPQDAIVDEA ARIVFDALR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg gcgtacgtgc gcagccggct tcctccgtat cgatcaatct tgctgcgcgt gagcgcttcg aacaactgga ggcatcgagt ggcggccggc tcggcgtcgc ggcgctcaga acggctgacg gttcctatgt cggctaccgg gagtcggaac gctttccgat gtgcagtact ttcaagctgc tcgccgtcgc attgatcctg aagcgcagca tggccgaacg cggcttgctc gaagagcgca tccgctacgg cgacgctcag gtcgtcgctc attcgcccgt cacgaagcgg cacgcaggcg gcgagggaat gacggtaggg gagttgagtg cagcggcgct ccagcatagc gacaacaccg cggccaatct gttgctgacg tcgcttggcg ggccggaggt gctgaaccag tttgcgttat cgatcggcga cacctggttc gacctcgttc gcaacgaacc cgaagtgaac gcatcggtgc ccggggacat gcgtgacacc acgtcgccgc gcgcgatgct gctcgatgtg cagaaactgc tgctcgcaga cgacgtgctg ggtgcgcgcg agcgcgagca gttgaaggcg tggatgctgg gcaatacgac gggtgccacg cgcatccgcg cggccgtgcc tgcggccgga tggttggtcg cggacaacac gggcagtgga gactacggca cggctaacga cgtcgccgtg gtatatccgc cctcgtcggc gccgttggtc gtcgcgatct acttcacggg cgtgacgccg aaggaaacgc cgccacagga cgcaatcgtc gacgaggcgg cgcgaatcgt attcgacgcg ctgcgatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BuphA.00104.a.B1.PS37967
| PURIFICATION DATe: | 7/6/2016 |
| CONCENTRATION: | 34.22mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHMIG VRAQPASSVS INXAARERFE QLEASSGGRL GVAALRTADG SYVGYRESER FPMCSTFKLL AVALILKRSM AERGLLEERI RYGDAQVVAH SPVTKRHAGG EGMTVGELSA AALQHSDNTA ANLLLTSLGG PEVLNQFALS IGDTWFDLVR NEPEVNASVP GDMRDTTSPR AMLLDVQKLL LADDVLGARE REQLKAWMLG NTTGATRIRA AVPAAGWLVA DNTGSGDYGT ANDVAVVYPP SSAPLVVAIY FTGVTPKETP PQDAIVDEAA XXVFDALR
Validated NT Sequence
atcttactca cttatcgcag cgcgtcgaat acgnnnnnng ccgcctcgtc gacgattgcg tcctgtggcg gcgtttcctt cggcgtcacg cccgtgaagt agatcgcgac gaccaacggc gccgacgagg gcggatatac cacggcgacg tcgttagccg tgccgtagtc tccactgccc gtgttgtccg cgaccaacca tccggccgca ggcacggccg cgcggatgcg cgtggcaccc gtcgtattgc ccagcatcca cgccttcaac tgctcgcgct cgcgcgcacc cagcacgtcg tctgcgagca gcagtttctg cacatcgagc agcatcgcgc gcggcgacgt ggtgtcacgc atgtccccgg gcaccgatgc gttcacttcg ggttcgttgc gaacgaggtc gaaccaggtg tcgccgatcg ataacgcaaa ctggttcagc acctccggcc cgccaagcga cgtcagcaac agattggccg cggtgttgtc gctatgctgg agcgccgctg cactcaactc ccctaccgtc attccctcgc cgcctgcgtg ccgcttcgtg acgggcgaat gagcgacgac ctgagcgtcg ccgtagcgga tgcgctcttc gagcaagccg cgttcggcca tgctgcgctt caggatcaat gcgacggcga gcagcttgaa agtactgcac atcggaaagc gttccgactc ccggtagccg acataggaac cgtcagccgt tctgagcgcc gcgacgccga gccggccgcc actcgatgcc tccagttgtt cgaagcgctc acgcgcagca anattgatcg atacggagga agccggctgc gcacgtacgc cgatcatatg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMI GVRAQPASSV SINLAARERF EQLEASSGGR LGVAALRTAD GSYVGYRESE RFPMCSTFKL LAVALILKRS MAERGLLEER IRYGDAQVVA HSPVTKRHAG GEGMTVGELS AAALQHSDNT AANLLLTSLG GPEVLNQFAL SIGDTWFDLV RNEPEVNASV PGDMRDTTSP RAMLLDVQKL LLADDVLGAR EREQLKAWML GNTTGATRIR AAVPAAGWLV ADNTGSGDYG TANDVAVVYP PSSAPLVVAI YFTGVTPKET PPQDAIVDEA ARIVFDALR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCG GCGTACGTGC GCAGCCGGCT TCCTCCGTAT CGATCAATCT TGCTGCGCGT GAGCGCTTCG AACAACTGGA GGCATCGAGT GGCGGCCGGC TCGGCGTCGC GGCGCTCAGA ACGGCTGACG GTTCCTATGT CGGCTACCGG GAGTCGGAAC GCTTTCCGAT GTGCAGTACT TTCAAGCTGC TCGCCGTCGC ATTGATCCTG AAGCGCAGCA TGGCCGAACG CGGCTTGCTC GAAGAGCGCA TCCGCTACGG CGACGCTCAG GTCGTCGCTC ATTCGCCCGT CACGAAGCGG CACGCAGGCG GCGAGGGAAT GACGGTAGGG GAGTTGAGTG CAGCGGCGCT CCAGCATAGC GACAACACCG CGGCCAATCT GTTGCTGACG TCGCTTGGCG GGCCGGAGGT GCTGAACCAG TTTGCGTTAT CGATCGGCGA CACCTGGTTC GACCTCGTTC GCAACGAACC CGAAGTGAAC GCATCGGTGC CCGGGGACAT GCGTGACACC ACGTCGCCGC GCGCGATGCT GCTCGATGTG CAGAAACTGC TGCTCGCAGA CGACGTGCTG GGTGCGCGCG AGCGCGAGCA GTTGAAGGCG TGGATGCTGG GCAATACGAC GGGTGCCACG CGCATCCGCG CGGCCGTGCC TGCGGCCGGA TGGTTGGTCG CGGACAACAC GGGCAGTGGA GACTACGGCA CGGCTAACGA CGTCGCCGTG GTATATCCGC CCTCGTCGGC GCCGTTGGTC GTCGCGATCT ACTTCACGGG CGTGACGCCG AAGGAAACGC CGCCACAGGA CGCAATCGTC GACGAGGCGG CGCGAATCGT ATTCGACGCG CTGCGATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T