SSGCID: Target Page

BuphA.00102.a

2-dehydro-3-deoxyphosphooctonate aldolase

CENTER ID:	BuphA.00102.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00102.a.A1.GE32270	Full length( BuphA.00102.a )		1	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.1531
RefSeq:	YP_001857668.1
UniProt:	B2JIX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLGDFEIGL DKPFFLIAGT CVVESEQMTI DTAGKLKEIC AKLKIPFIYK SSYDKANRSS GKSFRGLGMD EGLRILSEVK RQLGLHVLTD VHSEHEIEPV ASVVDVLQTP AFLCRQTDFI HACARSGKPV NIKKGQFLAP HDMKNVIDKA RDAAREAGLS EDRFMACERG VSFGYNNLVS DMRSLAIMRE TGAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAAVAT GIAGLFMETH PNPAEAKSDG PNAVPLHRMA DLLETLMTLD QAVKRTPFLE NDFN

NT Sequence

ATGAAACTGG GCGATTTCGA AATCGGCCTC GACAAGCCGT TTTTCCTGAT CGCGGGCACC TGTGTCGTCG AATCCGAACA GATGACGATC GACACGGCCG GCAAGCTGAA GGAAATCTGT GCGAAGCTGA AGATTCCGTT CATCTACAAG TCTTCGTACG ACAAGGCCAA TCGCAGCAGC GGTAAGTCTT TCCGTGGTTT GGGAATGGAC GAGGGATTGC GCATCCTCTC CGAAGTGAAG CGTCAGCTGG GCCTTCACGT CTTGACCGAC GTGCACAGCG AGCATGAGAT CGAGCCGGTT GCATCCGTCG TCGACGTGCT GCAGACGCCG GCGTTCCTAT GCCGTCAGAC GGACTTCATC CACGCGTGCG CGCGTTCGGG CAAGCCCGTT AACATCAAGA AGGGCCAGTT CCTCGCGCCG CACGACATGA AGAACGTGAT CGACAAGGCG CGCGATGCGG CGCGTGAAGC CGGTCTGTCG GAAGACCGCT TCATGGCGTG CGAGCGCGGT GTGTCGTTCG GCTATAACAA CCTCGTGTCG GACATGCGTT CGCTCGCGAT CATGCGCGAA ACGGGCGCGC CCGTCGTGTT CGACGCGACC CACTCGGTTC AATTGCCGGG CGGGCAGGGC ACGAGTTCGG GGGGGCAGCG CGAGTTCGTC CCCGTTCTGG CGCGCGCGGC CGTCGCAACG GGTATCGCGG GCCTGTTCAT GGAAACGCAT CCGAATCCGG CGGAAGCGAA GTCGGACGGT CCGAATGCTG TGCCGCTGCA TCGCATGGCC GATCTGCTCG AGACGCTGAT GACGCTCGAC CAGGCCGTCA AGCGCACACC GTTCCTCGAG AACGATTTCA AC

Details for BuphA.00102.a.A1.GE32270

HARVESTED ON:	7/28/2011
SEQUENCED ON:	8/3/2011
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttattagttg aaatcgttct cgaggaacgg tgtgcgcttg acggcctggt cgagcgtcat cagcgtctcg agcagatcgg ccatgcgatg cagcggcaca gcattcggac cgtccgactt cgcttccgcc ggattcggat gcgtttccat gaacaggccc gcgatacccg ttgcgacggc cgcgcgcgcc agaacgggga cgaactcgcg ctgccccccc gaactcgtgc cctgcccgcc cggcaattga accgagtggg tcgcgtcgaa cacgacgggc gcgcccgttt cgcgcatgat cgcgagcgaa cgcatgtccg acacgaggtt gttatagccg aacgacacac cgcgctcgca cgccatgaag cggtcttccg acagaccggc ttcacgcgcc gcatcgcgcg ccttgtcgat cacgttcttc atgtcgtgcg gcgcgaggaa ctggcccttc ttgatgttaa cgggcttgcc cgaacgcgcg cacgcgtgga tgaagtccgt ctgacggcat aggaacgccg gcgtctgcag cacgtcgacg acggatgcaa ccggctcgat ctcatgctcg ctgtgcacgt cggtcaagac gtgaaggccc agctgacgct tcacttcgga gaggatgcgc aatccctcgt ccattcccaa accacggaaa gacttaccgc tgctgcgatt ggccttgtcg tacgaagact tgtagatgaa cggaatcttc agcttcgcac agatttcctt cagcttgccg gccgtgtcga tcgtcatctg ttcggattcg acgacacagg tgcccgcgat caggaaaaac ggcttgtcga ggccgatttc gaaatcgccc agtttcatcg aaccaggacc ctgggtctga gcttc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKLGDFEIG LDKPFFLIAG TCVVESEQMT IDTAGKLKEI CAKLKIPFIY KSSYDKANRS SGKSFRGLGM DEGLRILSEV KRQLGLHVLT DVHSEHEIEP VASVVDVLQT PAFLCRQTDF IHACARSGKP VNIKKGQFLA PHDMKNVIDK ARDAAREAGL SEDRFMACER GVSFGYNNLV SDMRSLAIMR ETGAPVVFDA THSVQLPGGQ GTSSGGQREF VPVLARAAVA TGIAGLFMET HPNPAEAKSD GPNAVPLHRM ADLLETLMTL DQAVKRTPFL ENDFN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaactgggc gatttcgaaa tcggcctcga caagccgttt ttcctgatcg cgggcacctg tgtcgtcgaa tccgaacaga tgacgatcga cacggccggc aagctgaagg aaatctgtgc gaagctgaag attccgttca tctacaagtc ttcgtacgac aaggccaatc gcagcagcgg taagtctttc cgtggtttgg gaatggacga gggattgcgc atcctctccg aagtgaagcg tcagctgggc cttcacgtct tgaccgacgt gcacagcgag catgagatcg agccggttgc atccgtcgtc gacgtgctgc agacgccggc gttcctatgc cgtcagacgg acttcatcca cgcgtgcgcg cgttcgggca agcccgttaa catcaagaag ggccagttcc tcgcgccgca cgacatgaag aacgtgatcg acaaggcgcg cgatgcggcg cgtgaagccg gtctgtcgga agaccgcttc atggcgtgcg agcgcggtgt gtcgttcggc tataacaacc tcgtgtcgga catgcgttcg ctcgcgatca tgcgcgaaac gggcgcgccc gtcgtgttcg acgcgaccca ctcggttcaa ttgccgggcg ggcagggcac gagttcgggg gggcagcgcg agttcgtccc cgttctggcg cgcgcggccg tcgcaacggg tatcgcgggc ctgttcatgg aaacgcatcc gaatccggcg gaagcgaagt cggacggtcc gaatgctgtg ccgctgcatc gcatggccga tctgctcgag acgctgatga cgctcgacca ggccgtcaag cgcacaccgt tcctcgagaa cgatttcaac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa