SSGCID: Target Page

BuphA.00093.c

NADH:flavin oxidoreductase/NADH oxidase

CENTER ID:	BuphA.00093.c
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00093.c.B1.GE39391	Full length( BuphA.00093.c )		1	373

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.6605
RefSeq:	YP_001862373.1
UniProt:	B2JWK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDATSPILT SYNRNGIALR NRIAVAPMTR ITATEYGYPT QAMFDYYKRF AKGGFGLVTT EGIYTDKAFS QGYRFQPGLA DDEQAKAWST FNREMHEHGT PVFAQLMHAG ALSQGNVYRS HTVGPSAVRP KGEQMKFYFG QGLYAEPKEM TDAEIDEAIQ GFVGAARRAV NIAGFKGVEI HGANGYLLDQ FLTAGTNRRT DRWGGDTKAR VQLLVDVVEA VRLGIGDAVP VGIRISQGKV NDFGSRWSGG EADAEVIFGT LADAGIDFLH VTEFEAWQPA FEGTHDSLVT LAHRYAPAVT VIANGGLHTA ERINHALASG ANLVTVGRGA LANPDLPKLL ANRREPRTFD SSILQPIASI KPEELAMQIA AST

NT Sequence

ATGAGCGACG CAACCTCCCC CATCCTTACT TCGTATAACC GCAACGGCAT CGCATTACGC AACCGCATCG CCGTCGCCCC CATGACGCGT ATCACGGCCA CGGAATACGG CTATCCGACC CAAGCCATGT TCGACTACTA CAAGCGCTTC GCGAAAGGTG GTTTCGGCCT GGTAACGACG GAAGGCATCT ATACCGACAA GGCGTTCTCG CAAGGATACC GGTTTCAGCC AGGATTGGCC GACGACGAAC AGGCGAAGGC ATGGTCGACG TTCAATCGCG AGATGCATGA GCATGGGACC CCTGTGTTCG CGCAACTCAT GCATGCCGGG GCGTTGAGTC AAGGCAACGT TTATCGAAGC CATACCGTGG GCCCGTCGGC CGTCCGCCCG AAGGGCGAAC AGATGAAGTT CTACTTCGGC CAGGGACTCT ATGCTGAGCC AAAAGAGATG ACAGACGCTG AGATTGATGA GGCTATTCAG GGGTTTGTCG GCGCGGCACG ACGTGCCGTG AATATTGCCG GGTTCAAGGG CGTGGAAATT CACGGCGCTA ACGGGTATCT CCTCGACCAG TTCCTGACAG CAGGCACTAA TCGTCGGACC GACCGTTGGG GCGGCGACAC GAAGGCTCGC GTGCAACTGC TCGTCGATGT TGTCGAAGCA GTCAGGCTCG GCATCGGAGA TGCTGTACCC GTGGGTATCA GGATTTCACA AGGCAAGGTC AACGATTTCG GTTCCAGGTG GTCGGGTGGC GAGGCGGATG CCGAAGTGAT CTTCGGCACA CTTGCCGATG CAGGCATCGA CTTCCTTCAC GTCACGGAGT TCGAGGCCTG GCAGCCGGCC TTCGAAGGCA CGCACGATAG CCTCGTGACG CTGGCACACC GTTACGCGCC CGCCGTCACC GTCATCGCAA ACGGCGGCTT GCACACGGCT GAGCGTATCA ACCACGCGCT GGCCTCCGGT GCGAATCTGG TGACAGTCGG ACGCGGCGCG CTGGCGAATC CCGACCTGCC GAAACTGCTC GCAAACAGGC GCGAACCTCG TACATTCGAC AGTTCGATCC TTCAACCGAT CGCCAGCATC AAGCCCGAAG AACTCGCAAT GCAGATAGCC GCTTCAACG

Details for BuphA.00093.c.B1.GE39391

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS DATSPILTSY NRNGIALRNR IAVAPMTRIT ATEYGYPTQA MFDYYKRFAK GGFGLVTTEG IYTDKAFSQG YRFQPGLADD EQAKAWSTFN REMHEHGTPV FAQLMHAGAL SQGNVYRSHT VGPSAVRPKG EQMKFYFGQG LYAEPKEMTD AEIDEAIQGF VGAARRAVNI AGFKGVEIHG ANGYLLDQFL TAGTNRRTDR WGGDTKARVQ LLVDVVEAVR LGIGDAVPVG IRISQGKVND FGSRWSGGEA DAEVIFGTLA DAGIDFLHVT EFEAWQPAFE GTHDSLVTLA HRYAPAVTVI ANGGLHTAER INHALASGAN LVTVGRGALA NPDLPKLLAN RREPRTFDSS ILQPIASIKP EE

Validated NT Sequence

gagttcttcg ggcttgatgc tggcgatcgg ttgaaggatc gaactgtcga atgtacgagg ttcgcgcctg tttgcgagca gtttcggcag gtcgggattc gccagcgcgc cgcgtccgac tgtcaccaga ttcgcaccgg aggccagcgc gtggttgata cgctcagccg tgtgcaagcc gccgtttgcg atgacggtga cggcgggcgc gtaacggtgt gccagcgtca cgaggctatc gtgcgtgcct tcgaaggccg gctgccaggc ctcgaactcc gtgacgtgaa ggaagtcgat gcctgcatcg gcaagtgtgc cgaagatcac ttcggcatcc gcctcgccac ccgaccacct ggaaccgaaa tcgttgacct tgccttgtga aatcctgata cccacgggta cagcatctcc gatgccgagc ctgactgctt cgacaacatc gacgagcagt tgcacgcgag ccttcgtgtc gccgccccaa cggtcggtcc gacgattagt gcctgctgtc aggaactggt cgaggagata cccgttagcg ccgtgaattt ccacgccctt gaacccggca atattcacgg cacgtcgtgc cgcgccgaca aacccctgaa tagcctcatc aatctcagcg tctgtcatct cttttggctc agcatagagt ccctggccga agtagaactt catctgttcg cccttcgggc ggacggccga cgggcccacg gtatggcttc gataaacgtt gccttgactc aacgccccgg catgcatgag ttgcgcgaac acaggggtcc catgctcatg catctcgcga ttgaacgtcg accatgcctt cgcctgttcg tcgtcggcca atcctggctg aaaccggtat ccttgcgaga acgccttgtc ggtatagatg ccttccgtcg ttaccaggcc gaaaccacct ttcgcgaagc gcttgtagta gtcgaacatg gcttgggtcg gatagccgta ttccgtggcc gtgatacgcg tcatgggggc gacggcgatg cggttgcgta atgcgatgcc gttgcggtta tacgaagtaa ggatggggga ggttgcgtcg ctcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acgcaacctc ccccatcctt acttcgtata accgcaacgg catcgcatta cgcaaccgca tcgccgtcgc ccccatgacg cgtatcacgg ccacggaata cggctatccg acccaagcca tgttcgacta ctacaagcgc ttcgcgaaag gtggtttcgg cctggtaacg acggaaggca tctataccga caaggcgttc tcgcaaggat accggtttca gccaggattg gccgacgacg aacaggcgaa ggcatggtcg acgttcaatc gcgagatgca tgagcatggg acccctgtgt tcgcgcaact catgcatgcc ggggcgttga gtcaaggcaa cgtttatcga agccataccg tgggcccgtc ggccgtccgc ccgaagggcg aacagatgaa gttctacttc ggccagggac tctatgctga gccaaaagag atgacagacg ctgagattga tgaggctatt caggggtttg tcggcgcggc acgacgtgcc gtgaatattg ccgggttcaa gggcgtggaa attcacggcg ctaacgggta tctcctcgac cagttcctga cagcaggcac taatcgtcgg accgaccgtt ggggcggcga cacgaaggct cgcgtgcaac tgctcgtcga tgttgtcgaa gcagtcaggc tcggcatcgg agatgctgta cccgtgggta tcaggatttc acaaggcaag gtcaacgatt tcggttccag gtggtcgggt ggcgaggcgg atgccgaagt gatcttcggc acacttgccg atgcaggcat cgacttcctt cacgtcacgg agttcgaggc ctggcagccg gccttcgaag gcacgcacga tagcctcgtg acgctggcac accgttacgc gcccgccgtc accgtcatcg caaacggcgg cttgcacacg gctgagcgta tcaaccacgc gctggcctcc ggtgcgaatc tggtgacagt cggacgcggc gcgctggcga atcccgacct gccgaaactg ctcgcaaaca ggcgcgaacc tcgtacattc gacagttcga tccttcaacc gatcgccagc atcaagcccg aagaactcgc aatgcagata gccgcttcaa cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat