BuphA.00090.b

Phospho-2-dehydro-3-deoxyheptonate aldolase

CENTER ID: BuphA.00090.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00090.b.B1.GE40189 Full length( BuphA.00090.b ) 1 374
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4338
RefSeq: YP_001860278.1
UniProt: B2JPP1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRIDNPARD QEAGVADSTQ DTTRIDDTRI GAVRPLISPA LLLDELPCPP DVQTLVEKSR VEIADILHDR DDRLVVVVGP CSIHDHDQAM EYAHKLRTAA EALRDDLMIV MRVYFEKPRT TVGWKGYIND PRLDGSFRIN EGLRRARELL LEISGLGLPT GTEFLDLLSP QYIADLIAWG AIGARTTESQ SHRQLASGLS CPIGFKNGTD GGVQIAADAI VAAAASHAFM GMTKMGMAAI FETRGNDDAH VILRGGKKGP NYDAAGVEEA CTALRKVGLR EQVMVDCSHA NSNKSHERQI EVAQDLANQL SGGDKRIVGV MIESHLEAGR QDLKPGVPLA HGVSITDACI GWAQTEPVLE TLAQAVRARR QRNA
NT Sequence
GTGAGCCGCA TCGACAACCC CGCACGCGAC CAGGAAGCCG GCGTCGCCGA TTCCACCCAG GACACCACGC GCATCGACGA TACGCGCATC GGCGCGGTGC GTCCGCTGAT CTCACCTGCG CTGCTGCTCG ACGAACTGCC GTGCCCGCCG GACGTCCAGA CGCTCGTCGA GAAGAGCCGC GTCGAAATCG CCGACATCCT GCACGATCGC GACGACCGGC TGGTCGTCGT CGTTGGTCCG TGTTCGATTC ACGATCACGA TCAGGCAATG GAGTACGCGC ACAAGCTGAG AACCGCCGCC GAAGCATTGC GCGATGACCT GATGATCGTG ATGCGCGTGT ACTTCGAGAA GCCGCGTACG ACAGTCGGCT GGAAAGGCTA TATCAACGAT CCGCGTCTGG ACGGCAGCTT CCGCATCAAC GAAGGGCTGC GCCGCGCGCG TGAACTGCTG CTGGAGATCA GCGGCCTCGG CCTGCCGACG GGCACCGAGT TTCTCGATCT GCTGAGCCCG CAATACATCG CGGACCTGAT CGCGTGGGGC GCGATCGGCG CACGCACGAC GGAAAGCCAG AGTCACCGGC AGCTCGCGTC GGGCCTGTCG TGTCCGATCG GCTTCAAGAA CGGCACGGAC GGAGGCGTGC AGATCGCCGC CGACGCAATC GTCGCGGCAG CGGCGAGCCA CGCTTTCATG GGCATGACCA AGATGGGCAT GGCCGCCATC TTCGAGACGC GCGGCAACGA CGACGCGCAC GTCATCCTGC GCGGCGGCAA GAAAGGGCCG AACTACGACG CGGCGGGCGT CGAGGAAGCG TGCACCGCGC TGCGCAAGGT CGGCTTGCGC GAACAGGTGA TGGTGGACTG CTCGCACGCG AACTCGAACA AGTCGCATGA GCGGCAGATC GAAGTCGCAC AAGATCTGGC CAACCAGTTG TCGGGCGGCG ACAAGCGGAT CGTCGGCGTA ATGATCGAGA GCCATCTTGA AGCGGGCCGT CAGGATCTGA AGCCGGGCGT GCCGCTTGCT CACGGTGTAT CGATCACCGA CGCGTGCATC GGCTGGGCGC AAACGGAGCC CGTGCTTGAG ACGCTGGCGC AAGCCGTCCG CGCGCGCCGT CAACGCAACG CT
Details for BuphA.00090.b.B1.GE40189
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMV SRIDNPARDQ EAGVADSTQD TTRIDDTRIG AVRPLISPAL LLDELPCPPD VQTLVEKSRV EIADILHDRD DRLVVVVGPC SIHDHDQAME YAHKLRTAAE ALRDDLMIVM RVYFEKPRTT VGWKGYINDP RLDGSFRINE GLRRARELLL EISGLGLPTG TEFLDLLSPQ YIADLIAWGA IGARTTESQS HRQLASGLSC PIGFKNGTDG GVQIAADAIV AAAASHAFMG MTKMGMAAIF ETRGNDDAHV ILRGGKKGPN YDAAGVEEAC TALRKVGLRE QVMVDCSHAN SNKSHERQIE VAQDLANQLS GGDKRIVGVM IESHLEAGRQ DLKPGVPLAH GVSITDACIG WAQTEPVLET LAQA
Validated NT Sequence
acggcttgcg ccagcgtctc aagcacgggc tccgtttgcg cccagccgat gcacgcgtcg gtgatcgata caccgtgagc aagcggcacg cccggcttca gatcctgacg gcccgcttca agatggctct cgatcattac gccgacgatc cgcttgtcgc cgcccgacaa ctggttggcc agatcttgtg cgacttcgat ctgccgctca tgcgacttgt tcgagttcgc gtgcgagcag tccaccatca cctgttcgcg caagccgacc ttgcgcagcg cggtgcacgc ttcctcgacg cccgccgcgt cgtagttcgg ccctttcttg ccgccgcgca ggatgacgtg cgcgtcgtcg ttgccgcgcg tctcgaagat ggcggccatg cccatcttgg tcatgcccat gaaagcgtgg ctcgccgctg ccgcgacgat tgcgtcggcg gcgatctgca cgcctccgtc cgtgccgttc ttgaagccga tcggacacga caggcccgac gcgagctgcc ggtgactctg gctttccgtc gtgcgtgcgc cgatcgcgcc ccacgcgatc aggtccgcga tgtattgcgg gctcagcaga tcgagaaact cggtgcccgt cggcaggccg aggccgctga tctccagcag cagttcacgc gcgcggcgca gcccttcgtt gatgcggaag ctgccgtcca gacgcggatc gttgatatag cctttccagc cgactgtcgt acgcggcttc tcgaagtaca cgcgcatcac gatcatcagg tcatcgcgca atgcttcggc ggcggttctc agcttgtgcg cgtactccat tgcctgatcg tgatcgtgaa tcgaacacgg accaacgacg acgaccagcc ggtcgtcgcg atcgtgcagg atgtcggcga tttcgacgcg gctcttctcg acgagcgtct ggacgtccgg cgggcacggc agttcgtcga gcagcagcgc aggtgagatc agcggacgca ccgcgccgat gcgcgtatcg tcgatgcgcg tggtgtcctg ggtggaatcg gcgacgccgg cttcctggtc gcgtgcgggg ttgtcgatgc ggctcaccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS RIDNPARDQE AGVADSTQDT TRIDDTRIGA VRPLISPALL LDELPCPPDV QTLVEKSRVE IADILHDRDD RLVVVVGPCS IHDHDQAMEY AHKLRTAAEA LRDDLMIVMR VYFEKPRTTV GWKGYINDPR LDGSFRINEG LRRARELLLE ISGLGLPTGT EFLDLLSPQY IADLIAWGAI GARTTESQSH RQLASGLSCP IGFKNGTDGG VQIAADAIVA AAASHAFMGM TKMGMAAIFE TRGNDDAHVI LRGGKKGPNY DAAGVEEACT ALRKVGLREQ VMVDCSHANS NKSHERQIEV AQDLANQLSG GDKRIVGVMI ESHLEAGRQD LKPGVPLAHG VSITDACIGW AQTEPVLETL AQAVRARRQR NA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagcc gcatcgacaa ccccgcacgc gaccaggaag ccggcgtcgc cgattccacc caggacacca cgcgcatcga cgatacgcgc atcggcgcgg tgcgtccgct gatctcacct gcgctgctgc tcgacgaact gccgtgcccg ccggacgtcc agacgctcgt cgagaagagc cgcgtcgaaa tcgccgacat cctgcacgat cgcgacgacc ggctggtcgt cgtcgttggt ccgtgttcga ttcacgatca cgatcaggca atggagtacg cgcacaagct gagaaccgcc gccgaagcat tgcgcgatga cctgatgatc gtgatgcgcg tgtacttcga gaagccgcgt acgacagtcg gctggaaagg ctatatcaac gatccgcgtc tggacggcag cttccgcatc aacgaagggc tgcgccgcgc gcgtgaactg ctgctggaga tcagcggcct cggcctgccg acgggcaccg agtttctcga tctgctgagc ccgcaataca tcgcggacct gatcgcgtgg ggcgcgatcg gcgcacgcac gacggaaagc cagagtcacc ggcagctcgc gtcgggcctg tcgtgtccga tcggcttcaa gaacggcacg gacggaggcg tgcagatcgc cgccgacgca atcgtcgcgg cagcggcgag ccacgctttc atgggcatga ccaagatggg catggccgcc atcttcgaga cgcgcggcaa cgacgacgcg cacgtcatcc tgcgcggcgg caagaaaggg ccgaactacg acgcggcggg cgtcgaggaa gcgtgcaccg cgctgcgcaa ggtcggcttg cgcgaacagg tgatggtgga ctgctcgcac gcgaactcga acaagtcgca tgagcggcag atcgaagtcg cacaagatct ggccaaccag ttgtcgggcg gcgacaagcg gatcgtcggc gtaatgatcg agagccatct tgaagcgggc cgtcaggatc tgaagccggg cgtgccgctt gctcacggtg tatcgatcac cgacgcgtgc atcggctggg cgcaaacgga gcccgtgctt gagacgctgg cgcaagccgt ccgcgcgcgc cgtcaacgca acgcttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat