SSGCID: Target Page

BuphA.00090.a

Phospho-2-dehydro-3-deoxyheptonate aldolase

CENTER ID:	BuphA.00090.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00090.a.B1.GE40188	Full length( BuphA.00090.a )		1	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.2665
RefSeq:	YP_001858729.1
UniProt:	B2JGF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPPHNTDDVR IRELKELTPP AHLIREFPCD EKVSDLIFNA RQSMHRILHG MDDRLIVIVG PCSIHDTKAA LEYAGRLIEQ RKRFAGELEI VMRVYFEKPR TTVGWKGLIN DPYMDNSFKI NDGLRTAREL LMKINELGLP AGTEYLDMIS PQYIADLISW GAIGARTTES QVHRELASGL SCPVGFKNGT DGNVKIAVDA IKAASQPHHF LSVTKGGHSA IVSTAGNEDC HIILRGGKAP NYDADSVNAA CSDIGKAGLA ARLMIDASHA NSSKKHENQI PVCADIGRQI AAGDERIVGV MVESHLVAGR QDLVDGCALT YGQSITDACI GWDDSVAVLE GLADAVKQRR VARGSGN

NT Sequence

ATGCCCCCGC ACAACACCGA CGATGTCCGT ATCCGCGAAT TGAAAGAACT GACGCCGCCC GCGCACCTGA TCCGCGAATT TCCCTGCGAC GAAAAAGTGT CGGACCTGAT CTTCAACGCG CGTCAATCGA TGCATCGGAT TCTGCACGGC ATGGACGACC GGCTGATCGT GATCGTCGGG CCGTGCTCGA TTCACGACAC GAAGGCCGCG CTCGAGTACG CGGGGCGCCT GATCGAGCAA CGCAAGCGCT TCGCGGGCGA ACTGGAAATC GTGATGCGCG TGTACTTCGA AAAACCGCGC ACGACGGTGG GCTGGAAAGG CCTCATCAAC GACCCGTACA TGGACAACAG CTTCAAGATC AACGACGGCC TGCGCACCGC CCGCGAGCTG CTGATGAAGA TCAACGAGCT GGGGCTGCCC GCCGGCACCG AGTATCTCGA CATGATCAGC CCGCAATACA TCGCCGATCT GATCTCGTGG GGCGCGATCG GCGCGCGCAC GACGGAATCG CAGGTGCATC GCGAACTCGC GTCGGGGCTG TCGTGCCCGG TCGGCTTCAA GAACGGCACG GACGGCAACG TGAAGATCGC CGTCGACGCG ATCAAGGCCG CTTCGCAGCC TCACCATTTC CTGTCAGTGA CGAAGGGCGG CCATTCGGCC ATTGTCTCGA CGGCGGGCAA CGAGGATTGC CACATCATCC TGCGCGGCGG GAAGGCCCCG AACTACGACG CCGACAGCGT GAACGCCGCG TGCAGCGACA TCGGCAAGGC AGGCCTCGCC GCGCGTCTGA TGATCGACGC GAGCCACGCG AACAGCTCGA AGAAGCATGA AAACCAGATT CCCGTGTGCG CGGACATCGG CCGCCAGATC GCGGCGGGCG ACGAGCGCAT CGTCGGCGTG ATGGTTGAAT CGCATCTCGT CGCGGGCCGT CAGGATCTGG TGGACGGCTG CGCGCTGACG TACGGTCAGA GCATCACCGA TGCGTGCATC GGCTGGGACG ACAGCGTTGC CGTGCTCGAA GGTCTTGCAG ACGCCGTCAA GCAGCGGCGC GTCGCGCGCG GCAGCGGAAA C

Details for BuphA.00090.a.B1.GE40188

HARVESTED ON:	5/9/2016
SEQUENCED ON:	5/23/2016
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFPCDEK VSDLIFNARQ SMHRILHGMD DRLIVIVGPC SIHDTKAALE YAGRLIEQRK RFAGELEIVM RVYFEKPRTT VGWKGLINDP YMDNSFKIND GLRTARELLM KINELGLPAG TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHI ILRGGKAPNY DADSVNAACS DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQIAA GDERIVGVMV ESHLVAGRQD LVDGCALTYG QSITDACIGW DDSVAVLEGL ADAVKQ

Validated NT Sequence

gctgcttgac ggcgtctgca agaccttcga gcacggcaac gctgtcgtcc cagccgatgc acgcatcggt gatgctctga ccgtacgtca gcgcgcagcc gtccaccaga tcctgacggc ccgcgacgag atgcgattca accatcacgc cgacgatgcg ctcgtcgccc gccgcgatct ggcggccgat gtccgcgcac acgggaatct ggttttcatg cttcttcgag ctgttcgcgt ggctcgcgtc gatcatcaga cgcgcggcga ggcctgcctt gccgatgtcg ctgcacgcgg cgttcacgct gtcggcgtcg tagttcgggg ccttcccgcc gcgcaggatg atgtggcaat cctcgttgcc cgccgtcgag acaatggccg aatggccgcc cttcgtcact gacaggaaat ggtgaggctg cgaagcggcc ttgatcgcgt cgacggcgat cttcacgttg ccgtccgtgc cgttcttgaa gccgaccggg cacgacagcc ccgacgcgag ttcgcgatgc acctgcgatt ccgtcgtgcg cgcgccgatc gcgccccacg agatcagatc ggcgatgtat tgcgggctga tcatgtcgag atactcggtg ccggcgggca gccccagctc gttgatcttc atcagcagct cgcgggcggt gcgcaggccg tcgttgatct tgaagctgtt gtccatgtac gggtcgttga tgaggccttt ccagcccacc gtcgtgcgcg gtttttcgaa gtacacgcgc atcacgattt ccagttcgcc cgcgaagcgc ttgcgttgct cgatcaggcg ccccgcgtac tcgagcgcgg ccttcgtgtc gtgaatcgag cacggcccga cgatcacgat cagccggtcg tccatgccgt gcagaatccg atgcatcgat tgacgcgcgt tgaagatcag gtccgacact ttttcgtcgc agggaaattc gcggatcagg tgcgcgggcg gcgtcagttc tttcaattcg cggatacgga catcgtcggt gttgtgcggg ggcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccc cgcacaacac cgacgatgtc cgtatccgcg aattgaaaga actgacgccg cccgcgcacc tgatccgcga atttccctgc gacgaaaaag tgtcggacct gatcttcaac gcgcgtcaat cgatgcatcg gattctgcac ggcatggacg accggctgat cgtgatcgtc gggccgtgct cgattcacga cacgaaggcc gcgctcgagt acgcggggcg cctgatcgag caacgcaagc gcttcgcggg cgaactggaa atcgtgatgc gcgtgtactt cgaaaaaccg cgcacgacgg tgggctggaa aggcctcatc aacgacccgt acatggacaa cagcttcaag atcaacgacg gcctgcgcac cgcccgcgag ctgctgatga agatcaacga gctggggctg cccgccggca ccgagtatct cgacatgatc agcccgcaat acatcgccga tctgatctcg tggggcgcga tcggcgcgcg cacgacggaa tcgcaggtgc atcgcgaact cgcgtcgggg ctgtcgtgcc cggtcggctt caagaacggc acggacggca acgtgaagat cgccgtcgac gcgatcaagg ccgcttcgca gcctcaccat ttcctgtcag tgacgaaggg cggccattcg gccattgtct cgacggcggg caacgaggat tgccacatca tcctgcgcgg cgggaaggcc ccgaactacg acgccgacag cgtgaacgcc gcgtgcagcg acatcggcaa ggcaggcctc gccgcgcgtc tgatgatcga cgcgagccac gcgaacagct cgaagaagca tgaaaaccag attcccgtgt gcgcggacat cggccgccag atcgcggcgg gcgacgagcg catcgtcggc gtgatggttg aatcgcatct cgtcgcgggc cgtcaggatc tggtggacgg ctgcgcgctg acgtacggtc agagcatcac cgatgcgtgc atcggctggg acgacagcgt tgccgtgctc gaaggtcttg cagacgccgt caagcagcgg cgcgtcgcgc gcggcagcgg aaactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat