BuphA.00065.a

4-hydroxybenzoate 3-monooxygenase

CENTER ID: BuphA.00065.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00065.a.B1.GE40185 Full length( BuphA.00065.a ) 1 390
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00065.a.B1.PS38191 Full length( BuphA.00065.a ) 1 390

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4236
RefSeq: YP_001860183.1
UniProt: B2JPD4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRTQVGIIGA GPAGLLLSHL LHLQGIDSVV LESRSRDQIE STIRAGVLEQ GTMDLLTETG VGERMKAEGA LHHGFELAFE GKRRRIDLTD LTGKAITVYA QHEVIKDLVA ARMAAQGELR FGVSEVSLHD IDGAAPAIRY RHQGEAHELQ CDFIIGCDGS QGISRNAIPE ALRRDYQRVY PFGWFGILAE APPSSDELIY ARHERGFALV STRSPNVQRM YFQCDPSDNV DNWSDDRIWA EMHARVDSDE GHKVVEGKIF QKNIVGMRSF VSTTMQHGRL FLAGDAAHIV PPTGAKGLNL AVSDVRILSD ALRAFYKEDG SDQLERYSET ALKRIWRAEH FSYWMTRMMH RLDDASPFEQ QLQVAELEHV TTSRSAAISM AENYVGAVAV
NT Sequence
ATGCGTACGC AAGTCGGCAT CATTGGCGCT GGTCCTGCTG GACTGCTTCT CTCCCATCTT CTTCATCTGC AGGGTATTGA TTCCGTCGTG CTCGAATCGC GCAGCCGCGA TCAGATCGAA TCGACGATCC GCGCAGGCGT GCTCGAGCAG GGCACGATGG ATCTGCTCAC GGAAACCGGC GTAGGCGAGC GGATGAAAGC GGAAGGTGCG CTGCATCACG GCTTCGAACT CGCGTTTGAA GGCAAGCGCC GCCGCATCGA TCTCACTGAC CTCACGGGCA AAGCGATCAC GGTCTACGCG CAGCACGAAG TCATCAAGGA TCTGGTCGCC GCGCGTATGG CGGCGCAAGG GGAGTTGCGT TTCGGCGTGT CGGAAGTGTC GTTGCACGAT ATCGACGGTG CTGCGCCGGC TATCCGTTAT CGCCATCAAG GTGAAGCGCA TGAATTGCAA TGTGATTTCA TCATCGGCTG CGACGGCTCG CAGGGCATTT CGCGCAACGC GATTCCCGAA GCACTCCGCC GCGACTATCA GCGGGTTTAT CCGTTTGGCT GGTTCGGCAT TCTGGCCGAA GCGCCGCCCA GTTCCGACGA ATTGATCTAT GCGCGTCACG AGCGCGGCTT TGCGCTGGTC AGCACGCGCT CGCCGAACGT GCAGCGCATG TACTTCCAGT GCGACCCGAG CGACAACGTG GACAACTGGT CCGACGATCG CATCTGGGCG GAAATGCATG CACGCGTCGA TTCGGATGAA GGCCACAAGG TGGTTGAAGG CAAGATCTTC CAGAAGAACA TTGTCGGCAT GCGCAGTTTC GTGTCGACGA CGATGCAGCA CGGGCGCCTG TTCCTCGCCG GTGACGCAGC GCATATCGTG CCGCCCACAG GCGCGAAAGG TTTGAATCTC GCTGTTTCCG ACGTGCGCAT TTTGTCGGAT GCATTGCGCG CGTTCTACAA GGAAGACGGC AGCGATCAGC TGGAACGCTA TAGCGAAACG GCGCTGAAGC GAATCTGGCG CGCAGAACAT TTCTCGTACT GGATGACGCG CATGATGCAC CGTCTCGATG ATGCATCGCC ATTCGAGCAG CAATTGCAGG TTGCGGAACT CGAGCATGTG ACCACTTCGC GCAGCGCGGC GATTTCGATG GCCGAGAACT ACGTCGGCGC GGTGGCGGTT
Details for BuphA.00065.a.B1.GE40185
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMR TQVGIIGAGP AGLLLSHLLH LQGIDSVVLE SRSRDQIEST IRAGVLEQGT MDLLTETGVG ERMKAEGALH HGFELAFEGK RRRIDLTDLT GKAITVYAQH EVIKDLVAAR MAAQGELRFG VSEVSLHDID GAAPAIRYRH QGEAHELQCD FIIGCDGSQG ISRNAIPEAL RRDYQRVYPF GWFGILAEAP PSSDELIYAR HERGFALVST RSPNVQRMYF QCDPSDNVDN WSDDRIWAEM HARVDSDEGH KVVEGKIFQK NIVGMRSFVS TTMQHGRLFL AGDAAHIVPP TGAKGLNLAV SDVRILSDAL RAFYKEDGSD QLERYSETAL KRIWRAEHFS YWMTRMMHRL DDASPFEQQL QVAELEHVTT SRSAAISMA
Validated NT Sequence
cggccatcga aatcgccgcg ctgcgcgaag tggtcacatg ctcgagttcc gcaacctgca attgctgctc gaatggcgat gcatcatcga gacggtgcat catgcgcgtc atccagtacg agaaatgttc tgcgcgccag attcgcttca gcgccgtttc gctatagcgt tccagctgat cgctgccgtc ttccttgtag aacgcgcgca atgcatccga caaaatgcgc acgtcggaaa cagcgagatt caaacctttc gcgcctgtgg gcggcacgat atgcgctgcg tcaccggcga ggaacaggcg cccgtgctgc atcgtcgtcg acacgaaact gcgcatgccg acaatgttct tctggaagat cttgccttca accaccttgt ggccttcatc cgaatcgacg cgtgcatgca tttccgccca gatgcgatcg tcggaccagt tgtccacgtt gtcgctcggg tcgcactgga agtacatgcg ctgcacgttc ggcgagcgcg tgctgaccag cgcaaagccg cgctcgtgac gcgcatagat caattcgtcg gaactgggcg gcgcttcggc cagaatgccg aaccagccaa acggataaac ccgctgatag tcgcggcgga gtgcttcggg aatcgcgttg cgcgaaatgc cctgcgagcc gtcgcagccg atgatgaaat cacattgcaa ttcatgcgct tcaccttgat ggcgataacg gatagccggc gcagcaccgt cgatatcgtg caacgacact tccgacacgc cgaaacgcaa ctccccttgc gccgccatac gcgcggcgac cagatccttg atgacttcgt gctgcgcgta gaccgtgatc gctttgcccg tgaggtcagt gagatcgatg cggcggcgct tgccttcaaa cgcgagttcg aagccgtgat gcagcgcacc ttccgctttc atccgctcgc ctacgccggt ttccgtgagc agatccatcg tgccctgctc gagcacgcct gcgcggatcg tcgattcgat ctgatcgcgg ctgcgcgatt cgagcacgac ggaatcaata ccctgcagat gaagaagatg ggagagaagc agtccagcag gaccagcgcc aatgatgccg acttgcgtac gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMR TQVGIIGAGP AGLLLSHLLH LQGIDSVVLE SRSRDQIEST IRAGVLEQGT MDLLTETGVG ERMKAEGALH HGFELAFEGK RRRIDLTDLT GKAITVYAQH EVIKDLVAAR MAAQGELRFG VSEVSLHDID GAAPAIRYRH QGEAHELQCD FIIGCDGSQG ISRNAIPEAL RRDYQRVYPF GWFGILAEAP PSSDELIYAR HERGFALVST RSPNVQRMYF QCDPSDNVDN WSDDRIWAEM HARVDSDEGH KVVEGKIFQK NIVGMRSFVS TTMQHGRLFL AGDAAHIVPP TGAKGLNLAV SDVRILSDAL RAFYKEDGSD QLERYSETAL KRIWRAEHFS YWMTRMMHRL DDASPFEQQL QVAELEHVTT SRSAAISMAE NYVGAVAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgta cgcaagtcgg catcattggc gctggtcctg ctggactgct tctctcccat cttcttcatc tgcagggtat tgattccgtc gtgctcgaat cgcgcagccg cgatcagatc gaatcgacga tccgcgcagg cgtgctcgag cagggcacga tggatctgct cacggaaacc ggcgtaggcg agcggatgaa agcggaaggt gcgctgcatc acggcttcga actcgcgttt gaaggcaagc gccgccgcat cgatctcact gacctcacgg gcaaagcgat cacggtctac gcgcagcacg aagtcatcaa ggatctggtc gccgcgcgta tggcggcgca aggggagttg cgtttcggcg tgtcggaagt gtcgttgcac gatatcgacg gtgctgcgcc ggctatccgt tatcgccatc aaggtgaagc gcatgaattg caatgtgatt tcatcatcgg ctgcgacggc tcgcagggca tttcgcgcaa cgcgattccc gaagcactcc gccgcgacta tcagcgggtt tatccgtttg gctggttcgg cattctggcc gaagcgccgc ccagttccga cgaattgatc tatgcgcgtc acgagcgcgg ctttgcgctg gtcagcacgc gctcgccgaa cgtgcagcgc atgtacttcc agtgcgaccc gagcgacaac gtggacaact ggtccgacga tcgcatctgg gcggaaatgc atgcacgcgt cgattcggat gaaggccaca aggtggttga aggcaagatc ttccagaaga acattgtcgg catgcgcagt ttcgtgtcga cgacgatgca gcacgggcgc ctgttcctcg ccggtgacgc agcgcatatc gtgccgccca caggcgcgaa aggtttgaat ctcgctgttt ccgacgtgcg cattttgtcg gatgcattgc gcgcgttcta caaggaagac ggcagcgatc agctggaacg ctatagcgaa acggcgctga agcgaatctg gcgcgcagaa catttctcgt actggatgac gcgcatgatg caccgtctcg atgatgcatc gccattcgag cagcaattgc aggttgcgga actcgagcat gtgaccactt cgcgcagcgc ggcgatttcg atggccgaga actacgtcgg cgcggtggcg gtttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BuphA.00065.a.B1.PS38191
PURIFICATION DATe: 2/7/2017
CONCENTRATION: 45mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC buffer: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR TQVGIIGAGP AGLLLSHLLH LQGIDSVVLE SRSRDQIEST IRAGVLEQGT MDLLTETGVG ERMKAEGALH HGFELAFEGK RRRIDLTDLT GKAITVYAQH EVIKDLVAAR MAAQGELRFG VSEVSLHDID GAAPAIRYRH QGEAHELQCD FIIGCDGSQG ISRNAIPEAL RRDYQRVYPF GWFGILAEAP PSSDELIYAR HERGFALVST RSPNVQRMYF QCDPSDNVDN WSDDRIWAEM HARVDSDEGH KVVEGKIFQK NIVGMRSFVS TTMQHGRLFL AGDAAHIVPP TGAKGLNLAV SDVRILSDAL RAFYKEDGSD QLERYSETAL KRIWRAEHFS YWMTRMMHRL DDASPFEQQL QVAELEHVTT SRSAAISMA
Validated NT Sequence
cggccatcga aatcgccgcg ctgcgcgaag tggtcacatg ctcgagttcc gcaacctgca attgctgctc gaatggcgat gcatcatcga gacggtgcat catgcgcgtc atccagtacg agaaatgttc tgcgcgccag attcgcttca gcgccgtttc gctatagcgt tccagctgat cgctgccgtc ttccttgtag aacgcgcgca atgcatccga caaaatgcgc acgtcggaaa cagcgagatt caaacctttc gcgcctgtgg gcggcacgat atgcgctgcg tcaccggcga ggaacaggcg cccgtgctgc atcgtcgtcg acacgaaact gcgcatgccg acaatgttct tctggaagat cttgccttca accaccttgt ggccttcatc cgaatcgacg cgtgcatgca tttccgccca gatgcgatcg tcggaccagt tgtccacgtt gtcgctcggg tcgcactgga agtacatgcg ctgcacgttc ggcgagcgcg tgctgaccag cgcaaagccg cgctcgtgac gcgcatagat caattcgtcg gaactgggcg gcgcttcggc cagaatgccg aaccagccaa acggataaac ccgctgatag tcgcggcgga gtgcttcggg aatcgcgttg cgcgaaatgc cctgcgagcc gtcgcagccg atgatgaaat cacattgcaa ttcatgcgct tcaccttgat ggcgataacg gatagccggc gcagcaccgt cgatatcgtg caacgacact tccgacacgc cgaaacgcaa ctccccttgc gccgccatac gcgcggcgac cagatccttg atgacttcgt gctgcgcgta gaccgtgatc gctttgcccg tgaggtcagt gagatcgatg cggcggcgct tgccttcaaa cgcgagttcg aagccgtgat gcagcgcacc ttccgctttc atccgctcgc ctacgccggt ttccgtgagc agatccatcg tgccctgctc gagcacgcct gcgcggatcg tcgattcgat ctgatcgcgg ctgcgcgatt cgagcacgac ggaatcaata ccctgcagat gaagaagatg ggagagaagc agtccagcag gaccagcgcc aatgatgccg acttgcgtac gcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMR TQVGIIGAGP AGLLLSHLLH LQGIDSVVLE SRSRDQIEST IRAGVLEQGT MDLLTETGVG ERMKAEGALH HGFELAFEGK RRRIDLTDLT GKAITVYAQH EVIKDLVAAR MAAQGELRFG VSEVSLHDID GAAPAIRYRH QGEAHELQCD FIIGCDGSQG ISRNAIPEAL RRDYQRVYPF GWFGILAEAP PSSDELIYAR HERGFALVST RSPNVQRMYF QCDPSDNVDN WSDDRIWAEM HARVDSDEGH KVVEGKIFQK NIVGMRSFVS TTMQHGRLFL AGDAAHIVPP TGAKGLNLAV SDVRILSDAL RAFYKEDGSD QLERYSETAL KRIWRAEHFS YWMTRMMHRL DDASPFEQQL QVAELEHVTT SRSAAISMAE NYVGAVAV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTA CGCAAGTCGG CATCATTGGC GCTGGTCCTG CTGGACTGCT TCTCTCCCAT CTTCTTCATC TGCAGGGTAT TGATTCCGTC GTGCTCGAAT CGCGCAGCCG CGATCAGATC GAATCGACGA TCCGCGCAGG CGTGCTCGAG CAGGGCACGA TGGATCTGCT CACGGAAACC GGCGTAGGCG AGCGGATGAA AGCGGAAGGT GCGCTGCATC ACGGCTTCGA ACTCGCGTTT GAAGGCAAGC GCCGCCGCAT CGATCTCACT GACCTCACGG GCAAAGCGAT CACGGTCTAC GCGCAGCACG AAGTCATCAA GGATCTGGTC GCCGCGCGTA TGGCGGCGCA AGGGGAGTTG CGTTTCGGCG TGTCGGAAGT GTCGTTGCAC GATATCGACG GTGCTGCGCC GGCTATCCGT TATCGCCATC AAGGTGAAGC GCATGAATTG CAATGTGATT TCATCATCGG CTGCGACGGC TCGCAGGGCA TTTCGCGCAA CGCGATTCCC GAAGCACTCC GCCGCGACTA TCAGCGGGTT TATCCGTTTG GCTGGTTCGG CATTCTGGCC GAAGCGCCGC CCAGTTCCGA CGAATTGATC TATGCGCGTC ACGAGCGCGG CTTTGCGCTG GTCAGCACGC GCTCGCCGAA CGTGCAGCGC ATGTACTTCC AGTGCGACCC GAGCGACAAC GTGGACAACT GGTCCGACGA TCGCATCTGG GCGGAAATGC ATGCACGCGT CGATTCGGAT GAAGGCCACA AGGTGGTTGA AGGCAAGATC TTCCAGAAGA ACATTGTCGG CATGCGCAGT TTCGTGTCGA CGACGATGCA GCACGGGCGC CTGTTCCTCG CCGGTGACGC AGCGCATATC GTGCCGCCCA CAGGCGCGAA AGGTTTGAAT CTCGCTGTTT CCGACGTGCG CATTTTGTCG GATGCATTGC GCGCGTTCTA CAAGGAAGAC GGCAGCGATC AGCTGGAACG CTATAGCGAA ACGGCGCTGA AGCGAATCTG GCGCGCAGAA CATTTCTCGT ACTGGATGAC GCGCATGATG CACCGTCTCG ATGATGCATC GCCATTCGAG CAGCAATTGC AGGTTGCGGA ACTCGAGCAT GTGACCACTT CGCGCAGCGC GGCGATTTCG ATGGCCGAGA ACTACGTCGG CGCGGTGGCG GTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT