BuphA.00058.c

Thioredoxin-disulfide reductase

CENTER ID: BuphA.00058.c
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00058.c.A1.GE33063 Full length( BuphA.00058.c ) 1 411
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.7214
RefSeq: YP_001862946.1
UniProt: B2JTJ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVRIYGRMRS AHGYAIRDFL HRCDIPFEWI ELGSDKEAEE LARVQHLFDS RLPVCVFHDG TRLESPTIRQ ITEKLGWFAS PSREAYDLAI YGAGPAGLSA AVYGASEGLH TVLVERWALG GQAGSSSKIE NYLGFPQGVS GAELAERARD QAVKFGAEIL LARAGVHAEF PPGQGIVYLE DGTRITARTS ICATGVAYRT LGLAGEERFL GAGLYYGAGA SEAALIHGEN VYVVGGGNSA GQAAMHFSQS ANRVYMLVRD ASLKNTLSQY LVDRITSASN IEVRTCTEVT ALAGSDVLQE ITLTDVRTAT ARTVRTNWLF VCIGGVPQTQ WAQEVGIVRD EGGYLVTGPD LQEYRANLNW PLERAPLHLE TSVPGVFAAG DVRHASIKRV ASAVGEGAMA VALVHRYLNS S
NT Sequence
ATGGTCAGGA TCTATGGACG CATGCGCAGC GCACATGGCT ACGCGATACG CGACTTCCTG CATCGCTGTG ACATACCGTT CGAATGGATC GAACTGGGCA GCGATAAGGA AGCTGAAGAA CTGGCTCGCG TGCAGCATCT CTTCGATTCG CGCCTGCCCG TCTGCGTTTT CCACGACGGA ACGCGGCTCG AAAGTCCGAC CATCCGGCAA ATCACCGAGA AGCTTGGCTG GTTTGCGAGT CCATCCCGTG AGGCCTACGA TCTCGCGATT TACGGTGCAG GACCGGCGGG ATTGAGCGCC GCGGTGTACG GTGCATCGGA GGGACTGCAT ACCGTACTGG TGGAGCGCTG GGCACTGGGC GGACAGGCAG GTAGCAGTTC GAAGATCGAG AACTATCTGG GCTTTCCACA GGGCGTGAGC GGCGCGGAAC TTGCTGAACG GGCGCGCGAT CAGGCCGTCA AGTTCGGCGC CGAAATCCTG CTGGCGCGCG CCGGCGTGCA CGCGGAGTTC CCGCCCGGCC AGGGCATCGT ATATCTGGAA GACGGCACGC GTATCACGGC GCGCACGTCT ATCTGCGCGA CCGGCGTGGC ATATCGCACG CTAGGCCTGG CAGGTGAAGA GCGCTTTCTC GGCGCAGGTC TTTATTACGG CGCCGGCGCC AGTGAGGCCG CGCTGATACA TGGCGAAAAC GTATATGTGG TGGGCGGCGG CAATTCGGCG GGACAGGCCG CCATGCATTT TTCGCAGTCC GCCAACCGTG TCTATATGCT CGTGCGTGAC GCATCGCTCA AGAACACGTT GTCACAATAC CTGGTGGATC GCATCACATC GGCATCGAAC ATTGAAGTGC GCACATGCAC TGAAGTTACC GCGCTGGCAG GCAGCGACGT GTTGCAGGAG ATCACGCTGA CCGATGTGCG AACCGCCACG GCCCGTACGG TAAGAACGAA TTGGCTATTC GTCTGCATTG GCGGCGTGCC GCAGACGCAA TGGGCACAGG AAGTCGGCAT CGTTCGCGAT GAAGGCGGCT ATCTCGTAAC CGGTCCCGAT CTGCAGGAAT ACCGCGCGAA CCTGAATTGG CCTCTCGAAC GCGCGCCGCT GCATCTGGAA ACGAGTGTGC CTGGCGTATT CGCAGCGGGC GACGTGCGTC ACGCATCGAT CAAGCGCGTG GCGTCCGCAG TCGGCGAAGG GGCAATGGCT GTGGCGCTGG TGCATCGCTA TCTGAATAGC AGT
Details for BuphA.00058.c.A1.GE33063
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/23/2011
EXPECTED MW: 46kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 70
Validated AA Sequence
MGTLEAQTQG PGSMVRIYGR MRSAHGYAIR DFLHRCDIPF EWIELGSDKE AEELARVQHL FDSRLPVCVF HDGTRLESPT IRQITEKLGW FASPSREAYD LAIYGAGPAG LSAAVYGASE GLHTVLVERW ALGGQAGSSS KIENYLGFPQ GVSGAELAER ARDQAVKFGA EILLARAGVH AEFPPGQGIV YLEDGTRITA RTSICATGVA YRTLGLAGEE RFLGAXLYYG AGASEAALIX GENVYVVGGG NSAGXAAXHF SQSAXRVYML VRDASLXNTL SXYLVXRITS ASNXEVXTCX EXXAL
Validated NT Sequence
tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggt caggatctat ggacgcatgc gcagcgcaca tggctacgcg atacgcgact tcctgcatcg ctgtgacata ccgttcgaat ggatcgaact gggcagcgat aaggaagctg aagaactggc tcgcgtgcag catctcttcg attcgcgcct gcccgtctgc gttttccacg acggaacgcg gctcgaaagt ccgaccatcc ggcaaatcac cgagaagctt ggctggtttg cgagtccatc ccgtgaggcc tacgatctcg cgatttacgg tgcaggaccg gcgggattga gcgccgcggt gtacggtgca tcggagggac tgcataccgt actggtggag cgctgggcac tgggcggaca ggcaggtagc agttcgaaga tcgagaacta tctgggcttt ccacagggcg tgagcggcgc ggaacttgct gaacgggcgc gcgatcaggc cgtcaagttc ggcgccgaaa tcctgctggc gcgcgccggc gtgcacgcgg agttcccgcc cggccagggc atcgtatatc tggaagacgg cacgcgtatc acggcgcgca cgtctatctg cgcgaccggc gtggcatatc gcacgctagg cctggcaggt gaagagcgct ttctcggcgc angtctttat tacggcgccg gcgccagtga ggccgcgctg atacntggcg aaaacgtata tgtggtgggc ggcggcaatt cggcgggaca ngccgccntg catttttcgc agtccgccna ccgtgtctat atgctcgtgc gtgacgcatc gctcnanaac acgttgtcnc natacctggt ggnncgcatc acatcggcnt cgaacantga agtgngcaca tgcnctgaan ttnccgcgct gg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRIYGRMR SAHGYAIRDF LHRCDIPFEW IELGSDKEAE ELARVQHLFD SRLPVCVFHD GTRLESPTIR QITEKLGWFA SPSREAYDLA IYGAGPAGLS AAVYGASEGL HTVLVERWAL GGQAGSSSKI ENYLGFPQGV SGAELAERAR DQAVKFGAEI LLARAGVHAE FPPGQGIVYL EDGTRITART SICATGVAYR TLGLAGEERF LGAGLYYGAG ASEAALIHGE NVYVVGGGNS AGQAAMHFSQ SANRVYMLVR DASLKNTLSQ YLVDRITSAS NIEVRTCTEV TALAGSDVLQ EITLTDVRTA TARTVRTNWL FVCIGGVPQT QWAQEVGIVR DEGGYLVTGP DLQEYRANLN WPLERAPLHL ETSVPGVFAA GDVRHASIKR VASAVGEGAM AVALVHRYLN SS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtcaggatc tatggacgca tgcgcagcgc acatggctac gcgatacgcg acttcctgca tcgctgtgac ataccgttcg aatggatcga actgggcagc gataaggaag ctgaagaact ggctcgcgtg cagcatctct tcgattcgcg cctgcccgtc tgcgttttcc acgacggaac gcggctcgaa agtccgacca tccggcaaat caccgagaag cttggctggt ttgcgagtcc atcccgtgag gcctacgatc tcgcgattta cggtgcagga ccggcgggat tgagcgccgc ggtgtacggt gcatcggagg gactgcatac cgtactggtg gagcgctggg cactgggcgg acaggcaggt agcagttcga agatcgagaa ctatctgggc tttccacagg gcgtgagcgg cgcggaactt gctgaacggg cgcgcgatca ggccgtcaag ttcggcgccg aaatcctgct ggcgcgcgcc ggcgtgcacg cggagttccc gcccggccag ggcatcgtat atctggaaga cggcacgcgt atcacggcgc gcacgtctat ctgcgcgacc ggcgtggcat atcgcacgct aggcctggca ggtgaagagc gctttctcgg cgcaggtctt tattacggcg ccggcgccag tgaggccgcg ctgatacatg gcgaaaacgt atatgtggtg ggcggcggca attcggcggg acaggccgcc atgcattttt cgcagtccgc caaccgtgtc tatatgctcg tgcgtgacgc atcgctcaag aacacgttgt cacaatacct ggtggatcgc atcacatcgg catcgaacat tgaagtgcgc acatgcactg aagttaccgc gctggcaggc agcgacgtgt tgcaggagat cacgctgacc gatgtgcgaa ccgccacggc ccgtacggta agaacgaatt ggctattcgt ctgcattggc ggcgtgccgc agacgcaatg ggcacaggaa gtcggcatcg ttcgcgatga aggcggctat ctcgtaaccg gtcccgatct gcaggaatac cgcgcgaacc tgaattggcc tctcgaacgc gcgccgctgc atctggaaac gagtgtgcct ggcgtattcg cagcgggcga cgtgcgtcac gcatcgatca agcgcgtggc gtccgcagtc ggcgaagggg caatggctgt ggcgctggtg catcgctatc tgaatagcag ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa