BuphA.00058.b

Ferredoxin--NADP reductase

CENTER ID: BuphA.00058.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00058.b.A1.GE33062 Full length( BuphA.00058.b ) 1 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00058.b.A1.PS01345 Full length( BuphA.00058.b ) 1 351

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.3955
RefSeq: YP_001859921.1
UniProt: B2JN27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSSANIATD SPANTDVLII GAGPVGLFAA FEAGVIGLSS VIVDNVERAG GQCIELYPDK PIYDIPAIPS CTARELVDRL LEQCRPFAPP LHLGHRVDTV EQLDNGRWLA RTDKGLTVES AAILIAAGNG SFVPQRLQLD DAAPLEGHYV HYSVSNLADF AGKKMVVAGG GDSALDWALA LRTVAQHVTL VHRRNGFSAT DSSVANMRRA VEAGEMNFAV GTIASLNAPG GQLESIELRQ AEGSVQLATD HLLVLFGLVA DLGPIQQWGV EVRGGRITVD TSNYESSRPG IFAAGDIAGY PNKQKLILSG FHEASLALRK AYSYAYPDKK RVHIHSSYDA KLAERVAASH T
NT Sequence
ATGACAAGTT CGGCAAACAT TGCGACCGAC TCACCGGCCA ACACCGACGT GCTGATCATC GGCGCGGGCC CAGTGGGCCT GTTCGCCGCT TTCGAGGCAG GCGTGATCGG GCTTTCCAGC GTGATCGTCG ACAACGTCGA GCGCGCAGGC GGCCAGTGTA TCGAGCTGTA TCCGGACAAG CCGATCTACG ACATCCCCGC GATCCCCTCC TGCACCGCCC GAGAACTGGT CGACCGCCTG CTCGAGCAAT GCCGGCCTTT CGCTCCGCCG CTGCATCTCG GGCATCGCGT CGACACGGTC GAGCAGCTGG ATAACGGGCG CTGGCTCGCG CGTACCGACA AGGGACTCAC GGTTGAATCC GCCGCCATCC TGATTGCGGC CGGCAATGGG TCGTTCGTGC CGCAACGCTT GCAGCTCGAC GACGCAGCGC CGCTCGAAGG TCATTATGTT CACTACAGCG TAAGCAATCT CGCCGATTTC GCCGGGAAGA AAATGGTGGT GGCAGGGGGA GGCGACTCCG CGCTCGATTG GGCCTTGGCG CTTCGCACCG TCGCGCAGCA CGTCACGCTC GTGCACCGGC GCAATGGCTT CAGCGCGACG GACTCGAGCG TCGCCAACAT GCGCCGTGCC GTCGAGGCGG GCGAAATGAA TTTTGCGGTC GGCACGATTG CGAGCCTGAA CGCACCGGGT GGACAGCTCG AATCGATCGA GCTTCGTCAG GCCGAGGGAT CCGTGCAGCT GGCCACGGAT CATCTGCTCG TTCTGTTCGG TCTCGTCGCG GACCTGGGTC CGATCCAACA ATGGGGCGTC GAAGTGCGGG GCGGCCGCAT CACTGTCGAC ACCTCGAACT ACGAGAGTTC GCGTCCCGGC ATCTTCGCAG CAGGCGATAT CGCGGGCTAC CCGAACAAGC AGAAGCTGAT TCTGTCTGGC TTTCACGAAG CGTCGCTCGC GCTTCGGAAG GCGTACAGCT ACGCGTATCC GGACAAGAAA CGCGTGCATA TCCACTCGAG TTACGACGCC AAACTCGCAG AGCGCGTCGC CGCGTCTCAC ACC
Details for BuphA.00058.b.A1.GE33062
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/23/2011
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 18
PERCENT COVERAGE: 84
Validated AA Sequence
MHAFLVRIRV AVRLPKRERR FVKARQNQLL LVRVARDIAC CEDAGTRTLV VRGVDSDAAA PHFDAPLLDR TQVRDETEQN EQMIRGQLHG SLGLTKLDRF ELSTRCVQAR NRADRKIHFA RLDGTAHVGD ARVRRAEAIA PVHERDVLRD GAKRQGPIER GVASPCHHHF LPGEIGEIAY AVVNIMTFER RCVVELQALR HERPIAGRNQ DGGGFNRESL VGTREPAPRY PAARPCRRDA RDAAAERKAG IARAGGRPVL GRCRRGSRGC RRSACPDTAR YTGRLRARRC RRSRWKARSR LPRKRRTGPL GPRR
Validated NT Sequence
agacgcggcg acgcgctctg cgagtttggc gtcgtaactc gagtggatat gcacgcgttt cttgtccgga tacgcgtagc tgtacgcctt ccgaagcgcg agcgacgctt cgtgaaagcc agacagaatc agcttctgct tgttcgggta gcccgcgata tcgcctgctg cgaagatgcc gggacgcgaa ctctcgtagt tcgaggtgtc gacagtgatg cggccgcccc gcacttcgac gccccattgt tggatcggac ccaggtccgc gacgagaccg aacagaacga gcagatgatc cgtggccagc tgcacggatc cctcggcctg acgaagctcg atcgattcga gctgtccacc cggtgcgttc aggctcgcaa tcgtgccgac cgcaaaattc atttcgcccg cctcgacggc acggcgcatg ttggcgacgc tcgagtccgt cgcgctgaag ccattgcgcc ggtgcacgag cgtgacgtgc tgcgcgacgg tgcgaagcgc caaggcccaa tcgagcgcgg agtcgcctcc ccctgccacc accattttct tcccggcgaa atcggcgaga ttgcttacgc tgtagtgaac ataatgacct tcgagcggcg ctgcgtcgtc gagctgcaag cgttgcggca cgaacgaccc attgccggcc gcaatcagga tggcggcgga ttcaaccgtg agtcccttgt cggtacgcgc gagccagcgc cccgttatcc agctgctcga ccgtgtcgac gcgatgcccg agatgcagcg gcggagcgaa aggccggcat tgctcgagca ggcggtcgac cagttctcgg gcggtgcagg aggggatcgc ggggatgtcg tagatcggct tgtccggata cagctcgata cactggccgc ctgcgcgctc gacgttgtcg acgatcacgc tggaaagccc gatcacgcct gcctcgaaag cggcgaacag gcccactggg cccgcgccga tgatcagcac gtcggtgttg gccggtgagt cggtcgcaat gtttgccgaa cttgtcatcg aaccaggacc ctgggtctga gcttccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTSSANIAT DSPANTDVLI IGAGPVGLFA AFEAGVIGLS SVIVDNVERA GGQCIELYPD KPIYDIPAIP SCTARELVDR LLEQCRPFAP PLHLGHRVDT VEQLDNGRWL ARTDKGLTVE SAAILIAAGN GSFVPQRLQL DDAAPLEGHY VHYSVSNLAD FAGKKMVVAG GGDSALDWAL ALRTVAQHVT LVHRRNGFSA TDSSVANMRR AVEAGEMNFA VGTIASLNAP GGQLESIELR QAEGSVQLAT DHLLVLFGLV ADLGPIQQWG VEVRGGRITV DTSNYESSRP GIFAAGDIAG YPNKQKLILS GFHEASLALR KAYSYAYPDK KRVHIHSSYD AKLAERVAAS HT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaagttcg gcaaacattg cgaccgactc accggccaac accgacgtgc tgatcatcgg cgcgggccca gtgggcctgt tcgccgcttt cgaggcaggc gtgatcgggc tttccagcgt gatcgtcgac aacgtcgagc gcgcaggcgg ccagtgtatc gagctgtatc cggacaagcc gatctacgac atccccgcga tcccctcctg caccgcccga gaactggtcg accgcctgct cgagcaatgc cggcctttcg ctccgccgct gcatctcggg catcgcgtcg acacggtcga gcagctggat aacgggcgct ggctcgcgcg taccgacaag ggactcacgg ttgaatccgc cgccatcctg attgcggccg gcaatgggtc gttcgtgccg caacgcttgc agctcgacga cgcagcgccg ctcgaaggtc attatgttca ctacagcgta agcaatctcg ccgatttcgc cgggaagaaa atggtggtgg cagggggagg cgactccgcg ctcgattggg ccttggcgct tcgcaccgtc gcgcagcacg tcacgctcgt gcaccggcgc aatggcttca gcgcgacgga ctcgagcgtc gccaacatgc gccgtgccgt cgaggcgggc gaaatgaatt ttgcggtcgg cacgattgcg agcctgaacg caccgggtgg acagctcgaa tcgatcgagc ttcgtcaggc cgagggatcc gtgcagctgg ccacggatca tctgctcgtt ctgttcggtc tcgtcgcgga cctgggtccg atccaacaat ggggcgtcga agtgcggggc ggccgcatca ctgtcgacac ctcgaactac gagagttcgc gtcccggcat cttcgcagca ggcgatatcg cgggctaccc gaacaagcag aagctgattc tgtctggctt tcacgaagcg tcgctcgcgc ttcggaaggc gtacagctac gcgtatccgg acaagaaacg cgtgcatatc cactcgagtt acgacgccaa actcgcagag cgcgtcgccg cgtctcacac ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BuphA.00058.b.A1.PS01345
PURIFICATION DATe: 1/16/2012
CONCENTRATION: 92.77mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 18
PERCENT COVERAGE: 84
Protocol Notes
notes unavailable
Validated AA Sequence
MHAFLVRIRV AVRLPKRERR FVKARQNQLL LVRVARDIAC CEDAGTRTLV VRGVDSDAAA PHFDAPLLDR TQVRDETEQN EQMIRGQLHG SLGLTKLDRF ELSTRCVQAR NRADRKIHFA RLDGTAHVGD ARVRRAEAIA PVHERDVLRD GAKRQGPIER GVASPCHHHF LPGEIGEIAY AVVNIMTFER RCVVELQALR HERPIAGRNQ DGGGFNRESL VGTREPAPRY PAARPCRRDA RDAAAERKAG IARAGGRPVL GRCRRGSRGC RRSACPDTAR YTGRLRARRC RRSRWKARSR LPRKRRTGPL GPRR
Validated NT Sequence
agacgcggcg acgcgctctg cgagtttggc gtcgtaactc gagtggatat gcacgcgttt cttgtccgga tacgcgtagc tgtacgcctt ccgaagcgcg agcgacgctt cgtgaaagcc agacagaatc agcttctgct tgttcgggta gcccgcgata tcgcctgctg cgaagatgcc gggacgcgaa ctctcgtagt tcgaggtgtc gacagtgatg cggccgcccc gcacttcgac gccccattgt tggatcggac ccaggtccgc gacgagaccg aacagaacga gcagatgatc cgtggccagc tgcacggatc cctcggcctg acgaagctcg atcgattcga gctgtccacc cggtgcgttc aggctcgcaa tcgtgccgac cgcaaaattc atttcgcccg cctcgacggc acggcgcatg ttggcgacgc tcgagtccgt cgcgctgaag ccattgcgcc ggtgcacgag cgtgacgtgc tgcgcgacgg tgcgaagcgc caaggcccaa tcgagcgcgg agtcgcctcc ccctgccacc accattttct tcccggcgaa atcggcgaga ttgcttacgc tgtagtgaac ataatgacct tcgagcggcg ctgcgtcgtc gagctgcaag cgttgcggca cgaacgaccc attgccggcc gcaatcagga tggcggcgga ttcaaccgtg agtcccttgt cggtacgcgc gagccagcgc cccgttatcc agctgctcga ccgtgtcgac gcgatgcccg agatgcagcg gcggagcgaa aggccggcat tgctcgagca ggcggtcgac cagttctcgg gcggtgcagg aggggatcgc ggggatgtcg tagatcggct tgtccggata cagctcgata cactggccgc ctgcgcgctc gacgttgtcg acgatcacgc tggaaagccc gatcacgcct gcctcgaaag cggcgaacag gcccactggg cccgcgccga tgatcagcac gtcggtgttg gccggtgagt cggtcgcaat gtttgccgaa cttgtcatcg aaccaggacc ctgggtctga gcttccagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTSSANIAT DSPANTDVLI IGAGPVGLFA AFEAGVIGLS SVIVDNVERA GGQCIELYPD KPIYDIPAIP SCTARELVDR LLEQCRPFAP PLHLGHRVDT VEQLDNGRWL ARTDKGLTVE SAAILIAAGN GSFVPQRLQL DDAAPLEGHY VHYSVSNLAD FAGKKMVVAG GGDSALDWAL ALRTVAQHVT LVHRRNGFSA TDSSVANMRR AVEAGEMNFA VGTIASLNAP GGQLESIELR QAEGSVQLAT DHLLVLFGLV ADLGPIQQWG VEVRGGRITV DTSNYESSRP GIFAAGDIAG YPNKQKLILS GFHEASLALR KAYSYAYPDK KRVHIHSSYD AKLAERVAAS HT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAAGTTCG GCAAACATTG CGACCGACTC ACCGGCCAAC ACCGACGTGC TGATCATCGG CGCGGGCCCA GTGGGCCTGT TCGCCGCTTT CGAGGCAGGC GTGATCGGGC TTTCCAGCGT GATCGTCGAC AACGTCGAGC GCGCAGGCGG CCAGTGTATC GAGCTGTATC CGGACAAGCC GATCTACGAC ATCCCCGCGA TCCCCTCCTG CACCGCCCGA GAACTGGTCG ACCGCCTGCT CGAGCAATGC CGGCCTTTCG CTCCGCCGCT GCATCTCGGG CATCGCGTCG ACACGGTCGA GCAGCTGGAT AACGGGCGCT GGCTCGCGCG TACCGACAAG GGACTCACGG TTGAATCCGC CGCCATCCTG ATTGCGGCCG GCAATGGGTC GTTCGTGCCG CAACGCTTGC AGCTCGACGA CGCAGCGCCG CTCGAAGGTC ATTATGTTCA CTACAGCGTA AGCAATCTCG CCGATTTCGC CGGGAAGAAA ATGGTGGTGG CAGGGGGAGG CGACTCCGCG CTCGATTGGG CCTTGGCGCT TCGCACCGTC GCGCAGCACG TCACGCTCGT GCACCGGCGC AATGGCTTCA GCGCGACGGA CTCGAGCGTC GCCAACATGC GCCGTGCCGT CGAGGCGGGC GAAATGAATT TTGCGGTCGG CACGATTGCG AGCCTGAACG CACCGGGTGG ACAGCTCGAA TCGATCGAGC TTCGTCAGGC CGAGGGATCC GTGCAGCTGG CCACGGATCA TCTGCTCGTT CTGTTCGGTC TCGTCGCGGA CCTGGGTCCG ATCCAACAAT GGGGCGTCGA AGTGCGGGGC GGCCGCATCA CTGTCGACAC CTCGAACTAC GAGAGTTCGC GTCCCGGCAT CTTCGCAGCA GGCGATATCG CGGGCTACCC GAACAAGCAG AAGCTGATTC TGTCTGGCTT TCACGAAGCG TCGCTCGCGC TTCGGAAGGC GTACAGCTAC GCGTATCCGG ACAAGAAACG CGTGCATATC CACTCGAGTT ACGACGCCAA ACTCGCAGAG CGCGTCGCCG CGTCTCACAC CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA