BuphA.00057.r

ABC transporter related

CENTER ID: BuphA.00057.r
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00057.r.A1.GE33060 Full length( BuphA.00057.r ) 1 371
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.7727
RefSeq: YP_001863455.1
UniProt: B2JXP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEVQLESVR KSYGGTEILH DIDLSIRHGE FVVFVGPSGS GKSTLLRMIG GLEQITGGRL SIDNQVVNHL DAADRNLGMV FQSYALYPHL SVRDNLAFPL RMAKVPRDAI GPRVEEVAGM LQIAHLLDRR PKQLSGGQRQ RVAIGRAIVR EPKVFLFDEP LSNLDTELRV QMRVQIAKLH RELGNTMIYV THDQVEAMTM ADKIVVLRNG HIEQVGTPHE LYHQPSSQFV AGFIGSPRMN FLKAACVNTG PGGVTVSLSS AAPVVLPVEA NGVSPGQAVT VGVRPDDFTA PTSDHDQLRV ELDVDFVEHL GNATYLYGTV GGEALVARAP AEGNFAAPMG RVSLSVARGD CHLFDASGQA LRRRNVPRAW N
NT Sequence
ATGGCTGAAG TTCAACTCGA AAGTGTCCGG AAGAGCTATG GTGGAACGGA AATTCTCCAC GACATCGATC TGTCGATCCG CCACGGCGAA TTCGTTGTCT TCGTGGGTCC GTCGGGATCA GGCAAGTCGA CGCTGTTGCG CATGATCGGG GGCCTCGAGC AGATCACCGG CGGACGGCTT TCCATCGATA ACCAGGTGGT CAATCATCTC GATGCGGCTG ATCGAAATCT CGGCATGGTG TTTCAGAGCT ACGCCCTTTA CCCGCATCTA AGCGTGCGGG ACAACCTGGC GTTTCCGCTG CGCATGGCGA AGGTGCCTCG TGACGCGATC GGGCCGCGGG TTGAAGAAGT CGCCGGCATG CTTCAGATCG CGCACCTGCT CGATCGTCGG CCAAAGCAGC TCTCGGGCGG TCAGCGCCAG CGTGTGGCGA TCGGGCGCGC CATCGTCAGG GAACCCAAGG TCTTTCTCTT TGACGAGCCG CTGTCGAACC TCGATACGGA GCTTCGCGTG CAGATGCGTG TGCAGATCGC GAAGCTGCAC CGTGAACTCG GCAACACGAT GATCTATGTC ACCCATGATC AGGTCGAGGC AATGACCATG GCCGACAAGA TCGTCGTACT TCGGAACGGC CATATCGAGC AGGTCGGAAC GCCGCACGAG CTTTATCATC AGCCCAGCTC ACAGTTTGTG GCCGGATTCA TCGGGTCCCC GCGTATGAAT TTTCTCAAGG CAGCCTGTGT GAACACGGGC CCCGGAGGGG TGACCGTGAG TCTGAGCAGC GCGGCCCCAG TCGTGCTGCC GGTCGAGGCG AACGGTGTTT CACCCGGTCA GGCTGTCACG GTCGGCGTGC GTCCGGATGA CTTCACCGCG CCAACCAGTG ACCACGATCA GCTGCGCGTG GAGCTGGATG TCGACTTTGT CGAACATCTC GGCAACGCGA CTTATCTCTA TGGAACAGTG GGCGGTGAGG CACTTGTTGC ACGAGCTCCT GCGGAAGGAA ACTTCGCAGC TCCAATGGGG CGAGTCTCGC TGTCAGTTGC GAGGGGCGAT TGCCACCTCT TTGACGCAAG CGGGCAAGCG TTGCGGCGCA GGAATGTCCC ACGGGCGTGG AAT
Details for BuphA.00057.r.A1.GE33060
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 106
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEVQLESV RKSYGGTEIL HDIDLSIRHG EFVVFVGPSG SGKSTLLRMI GGLEQITGGR LSIDNQVVNH LDAADRNLGM VFQSYALYPH LSVRDNLAFP LRMAKVPRDA IGPRVEEVAG MLQIAHLLDR RPKQLSGGQR QRVAIGRAIV REPKVFLFDE PLSNLDTELR VQMRVQIAKL HRELGNTMIY VTHDQVEAMT MADKIVVLRN GHIEQVGTPH ELYHQPSSQF VAGFIGSPRM NFLKAACVNT GPGXVXVSLS SAAPVVLPVE ANGVSPGQAV TVGVRPDDFT APTSDHDQLX VELDVDFVEH LGNATYLYXX VGGEXLXXXX XXXKLXXXXG XXXXXXXXXG XLXXXXXXXX XXXXXXXPXG XXXXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggctga agttcaactc gaaagtgtcc ggaagagcta tggtggaacg gaaattctcc acgacatcga tctgtcgatc cgccacggcg aattcgttgt cttcgtgggt ccgtcgggat caggcaagtc gacgctgttg cgcatgatcg ggggcctcga gcagatcacc ggcggacggc tttccatcga taaccaggtg gtcaatcatc tcgatgcggc tgatcgaaat ctcggcatgg tgtttcagag ctacgccctt tacccgcatc taagcgtgcg ggacaacctg gcgtttccgc tgcgcatggc gaaggtgcct cgtgacgcga tcgggccgcg ggttgaagaa gtcgccggca tgcttcagat cgcgcacctg ctcgatcgtc ggccaaagca gctctcgggc ggtcagcgcc agcgtgtggc gatcgggcgc gccatcgtca gggaacccaa ggtctttctc tttgacgagc cgctgtcgaa cctcgatacg gagcttcgcg tgcagatgcg tgtgcagatc gcgaagctgc accgtgaact cggcaacacg atgatctatg tcacccatga tcaggtcgag gcaatgacca tggccgacaa gatcgtcgta cttcggaacg gccatatcga gcaggtcgga acgccgcacg agctttatca tcagcccagc tcacagtttg tggccggatt catcgggtcc ccgcgtatga attttctcaa ggcagcctgt gtgaacacgg gccccggang ggtgancgtg agtctgagca gcgcggcccc agtcgtgctg ccggtcgagg cgaacggtgt ttcacccggt caggctgtca cggtcggcgt gcgtccggat gacttcaccg cgccaaccag tgaccacgat cagctgcncg tggagctgga tgtcgacttt gtcgaacatc tcggcaacgc gacttatctc tatnnancag tgggcggtga ggnacttgnt gnnngagnnn cngnnnnnaa acttcncanc nncnangggg nnnngtnnnn nntgncantt nnnnnggggn nattgcnnnc nnnnntnnnn nnnagnnnnn nannnnnnnn nnnnannnnc ccnnngggnn nnnnntnnnn aannnncncn annannnnnn ngnnnnnnnn nnnnnnnngn nnnnnnnnnn annaannnnn nngnnnnnnn nnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEVQLESV RKSYGGTEIL HDIDLSIRHG EFVVFVGPSG SGKSTLLRMI GGLEQITGGR LSIDNQVVNH LDAADRNLGM VFQSYALYPH LSVRDNLAFP LRMAKVPRDA IGPRVEEVAG MLQIAHLLDR RPKQLSGGQR QRVAIGRAIV REPKVFLFDE PLSNLDTELR VQMRVQIAKL HRELGNTMIY VTHDQVEAMT MADKIVVLRN GHIEQVGTPH ELYHQPSSQF VAGFIGSPRM NFLKAACVNT GPGGVTVSLS SAAPVVLPVE ANGVSPGQAV TVGVRPDDFT APTSDHDQLR VELDVDFVEH LGNATYLYGT VGGEALVARA PAEGNFAAPM GRVSLSVARG DCHLFDASGQ ALRRRNVPRA WN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgaagtt caactcgaaa gtgtccggaa gagctatggt ggaacggaaa ttctccacga catcgatctg tcgatccgcc acggcgaatt cgttgtcttc gtgggtccgt cgggatcagg caagtcgacg ctgttgcgca tgatcggggg cctcgagcag atcaccggcg gacggctttc catcgataac caggtggtca atcatctcga tgcggctgat cgaaatctcg gcatggtgtt tcagagctac gccctttacc cgcatctaag cgtgcgggac aacctggcgt ttccgctgcg catggcgaag gtgcctcgtg acgcgatcgg gccgcgggtt gaagaagtcg ccggcatgct tcagatcgcg cacctgctcg atcgtcggcc aaagcagctc tcgggcggtc agcgccagcg tgtggcgatc gggcgcgcca tcgtcaggga acccaaggtc tttctctttg acgagccgct gtcgaacctc gatacggagc ttcgcgtgca gatgcgtgtg cagatcgcga agctgcaccg tgaactcggc aacacgatga tctatgtcac ccatgatcag gtcgaggcaa tgaccatggc cgacaagatc gtcgtacttc ggaacggcca tatcgagcag gtcggaacgc cgcacgagct ttatcatcag cccagctcac agtttgtggc cggattcatc gggtccccgc gtatgaattt tctcaaggca gcctgtgtga acacgggccc cggaggggtg accgtgagtc tgagcagcgc ggccccagtc gtgctgccgg tcgaggcgaa cggtgtttca cccggtcagg ctgtcacggt cggcgtgcgt ccggatgact tcaccgcgcc aaccagtgac cacgatcagc tgcgcgtgga gctggatgtc gactttgtcg aacatctcgg caacgcgact tatctctatg gaacagtggg cggtgaggca cttgttgcac gagctcctgc ggaaggaaac ttcgcagctc caatggggcg agtctcgctg tcagttgcga ggggcgattg ccacctcttt gacgcaagcg ggcaagcgtt gcggcgcagg aatgtcccac gggcgtggaa ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa