SSGCID: Target Page

BuphA.00057.q

ABC transporter related

CENTER ID:	BuphA.00057.q
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00057.q.A1.GE33059	Full length( BuphA.00057.q )		1	237

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.4719
RefSeq:	YP_001860624.1
UniProt:	B2JQN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNAPMLKVER LNAYYGRAHI LFDVSLEVGR GEVVALMGRN GAGKSTTMKS IMGLLPRREG TVHFNSADIS ALPPHRIARM GLGYVPENRR VFAGLTVMEN LDTGRQPSRA GAPSWTPEKL FKLFPNLGEM PKRRGDQMSG GEQQMLTVSR TLMGNPYLVL LDEPSEGVAP VVVEQMANMI LELKREGLSI LLSEQNVHFA ELVSDRAYVL EKGQIRFSGT MRELAQDDVV RRAHLGV

NT Sequence

ATGAACGCAC CGATGCTGAA AGTGGAACGC CTGAACGCGT ACTACGGGCG CGCGCATATT CTCTTCGACG TGAGTCTGGA AGTAGGGCGC GGCGAAGTGG TCGCGCTGAT GGGCCGCAAC GGCGCGGGCA AATCGACGAC GATGAAGTCG ATCATGGGCC TCTTGCCGCG CCGTGAAGGC ACGGTCCACT TCAACAGCGC GGATATCTCC GCGCTGCCGC CGCATCGCAT CGCGCGCATG GGTTTGGGCT ATGTGCCGGA AAACCGCCGC GTGTTTGCCG GGCTCACGGT GATGGAGAAT CTCGATACCG GGCGCCAGCC GTCGCGTGCG GGCGCGCCGT CGTGGACGCC GGAGAAGCTT TTCAAGCTGT TCCCGAACCT CGGCGAGATG CCGAAGCGTC GCGGCGACCA GATGAGCGGC GGGGAGCAGC AGATGCTCAC CGTGTCGCGC ACGCTGATGG GCAACCCGTA TCTGGTGCTG CTCGACGAGC CCTCCGAAGG CGTCGCGCCC GTGGTCGTCG AACAGATGGC GAACATGATT CTCGAACTGA AGCGCGAGGG TCTGTCCATT CTGCTGTCCG AGCAGAACGT GCATTTCGCC GAACTGGTCA GCGATCGCGC GTACGTGCTC GAAAAAGGCC AGATCCGTTT CAGCGGAACG ATGCGCGAAC TTGCGCAAGA CGATGTCGTC CGGCGCGCGC ACCTGGGCGT T

Details for BuphA.00057.q.A1.GE33059

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNAPMLKVE RLNAYYGRAH ILFDVSLEVG RGEVVALMGR NGAGKSTTMK SIMGLLPRRE GTVHFNSADI SALPPHRIAR MGLGYVPENR RVFAGLTVME NLDTGRQPSR AGAPSWTPEK LFKLFPNLGE MPKRRGDQMS GGEQQMLTVS RTLMGNPYLV LLDEPSEGVA PVVVEQMANM ILELKREGLS ILLSEQNVHF AELVSDRAYV LEKGQIRFSG TMRELAQDDV VRRAHLGV

Validated NT Sequence

ttttntnnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaacgc accgatgctg aaagtggaac gcctgaacgc gtactacggg cgcgcgcata ttctcttcga cgtgagtctg gaagtagggc gcggcgaagt ggtcgcgctg atgggccgca acggcgcggg caaatcgacg acgatgaagt cgatcatggg cctcttgccg cgccgtgaag gcacggtcca cttcaacagc gcggatatct ccgcgctgcc gccgcatcgc atcgcgcgca tgggtttggg ctatgtgccg gaaaaccgcc gcgtgtttgc cgggctcacg gtgatggaga atctcgatac cgggcgccag ccgtcgcgtg cgggcgcgcc gtcgtggacg ccggagaagc ttttcaagct gttcccgaac ctcggcgaga tgccgaagcg tcgcggcgac cagatgagcg gcggggagca gcagatgctc accgtgtcgc gcacgctgat gggcaacccg tatctggtgc tgctcgacga gccctccgaa ggcgtcgcgc ccgtggtcgt cgaacagatg gcgaacatga ttctcgaact gaagcgcgag ggtctgtcca ttctgctgtc cgagcagaac gtgcatttcg ccgaactggt cagcgatcgc gcgtacgtgc tcgaaaaagg ccagatccgt ttcagcggaa cgatgcgcga acttgcgcaa gacgatgtcg tccggcgcgc gcacctgggc gtttaataaa cagcacgaac aagttctgca gccnagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gncnccgctn nnnnannnnn nnnnnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgcaccg atgctgaaag tggaacgcct gaacgcgtac tacgggcgcg cgcatattct cttcgacgtg agtctggaag tagggcgcgg cgaagtggtc gcgctgatgg gccgcaacgg cgcgggcaaa tcgacgacga tgaagtcgat catgggcctc ttgccgcgcc gtgaaggcac ggtccacttc aacagcgcgg atatctccgc gctgccgccg catcgcatcg cgcgcatggg tttgggctat gtgccggaaa accgccgcgt gtttgccggg ctcacggtga tggagaatct cgataccggg cgccagccgt cgcgtgcggg cgcgccgtcg tggacgccgg agaagctttt caagctgttc ccgaacctcg gcgagatgcc gaagcgtcgc ggcgaccaga tgagcggcgg ggagcagcag atgctcaccg tgtcgcgcac gctgatgggc aacccgtatc tggtgctgct cgacgagccc tccgaaggcg tcgcgcccgt ggtcgtcgaa cagatggcga acatgattct cgaactgaag cgcgagggtc tgtccattct gctgtccgag cagaacgtgc atttcgccga actggtcagc gatcgcgcgt acgtgctcga aaaaggccag atccgtttca gcggaacgat gcgcgaactt gcgcaagacg atgtcgtccg gcgcgcgcac ctgggcgttt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa