BuphA.00057.o

ABC transporter related

CENTER ID: BuphA.00057.o
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00057.o.A1.GE33057 Full length( BuphA.00057.o ) 1 354
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4278
RefSeq: YP_001860224.1
UniProt: B2JPI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAVELSGIY KRYGDTQVVH GIDLHIDDGE FVVLVGPSGC GKSTLMRMVA GLEEISGGEL TIGGTRANGL APQQRNISMV FQSYALYPHL SVYDNIAFGP RIRKEASTSF KPRIEAAAKM LNLSGYLDRL PRALSGGQRQ RVAMGRAVVR EPSLFLFDEP LSNLDAKLRV QMRTEIKALH QRLKNTVIYV THDQIEAMTM ADRIVVMNAG RIEQIGRPLE LYDRPANLFV ASFLGSPSMN FSAGEVVSAA NGVALKLGDG EQIRLPGAPV TPGAKVTLGV RPEHIETSTE GIPMDVEVIE PTGAETHLYG KIGGASWCVT TRERASIEPG QRVTVRFPEQ HMHLFDTESG RRLG
NT Sequence
ATGGCAGCAG TGGAACTGAG CGGAATCTAC AAACGTTACG GCGACACGCA GGTCGTGCAC GGCATCGACC TGCATATCGA CGACGGCGAG TTCGTCGTGC TGGTCGGCCC GTCGGGTTGC GGCAAGAGCA CGCTGATGCG GATGGTCGCG GGACTCGAGG AAATCAGCGG CGGCGAACTG ACGATCGGCG GCACGCGCGC GAACGGCTTG GCGCCGCAGC AGCGCAATAT TTCGATGGTG TTCCAGAGCT ACGCGCTGTA TCCGCATTTG TCCGTTTACG ACAACATCGC GTTCGGCCCG CGCATCCGCA AGGAAGCGTC GACGAGTTTC AAGCCGCGCA TCGAGGCTGC CGCGAAGATG CTGAACCTGA GCGGTTATCT GGACCGGTTG CCGCGCGCAC TGTCGGGCGG TCAGCGCCAG CGCGTCGCGA TGGGGCGCGC GGTGGTGCGC GAGCCGTCGC TGTTCCTGTT CGACGAACCG CTCTCGAATC TCGATGCAAA GCTCCGCGTG CAGATGCGCA CGGAAATCAA GGCGCTGCAT CAGCGGTTGA AGAACACGGT CATCTACGTC ACGCACGATC AGATTGAAGC GATGACGATG GCCGATCGCA TCGTCGTGAT GAACGCGGGG CGCATCGAGC AGATCGGGCG TCCGCTGGAG CTTTACGACA GGCCGGCGAA TCTTTTCGTC GCGAGCTTTC TCGGTTCGCC GTCGATGAAT TTTTCCGCGG GCGAAGTGGT GAGCGCGGCG AACGGCGTTG CGTTGAAGCT CGGTGACGGC GAGCAGATAC GGCTGCCCGG CGCACCCGTG ACGCCCGGCG CGAAAGTAAC GCTCGGTGTG CGGCCCGAGC ACATCGAAAC ATCGACTGAA GGCATTCCGA TGGATGTCGA GGTGATCGAG CCGACGGGCG CGGAAACGCA TCTGTACGGC AAGATCGGCG GCGCGTCGTG GTGCGTGACG ACGCGCGAGC GGGCGTCGAT CGAGCCCGGT CAGCGCGTGA CGGTGCGCTT TCCCGAGCAG CACATGCATC TGTTCGATAC GGAGAGTGGA CGCAGATTGG GC
Details for BuphA.00057.o.A1.GE33057
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAVELSGI YKRYGDTQVV HGIDLHIDDG EFVVLVGPSG CGKSTLMRMV AGLEEISGGE LTIGGTRANG LAPQQRNISM VFQSYALYPH LSVYDNIAFG PRIRKEASTS FKPRIEAAAK MLNLSGYLDR LPRALSGGQR QRVAMGRAVV REPSLFLFDE PLSNLDAKLR VQMRTEIKAL HQRLKNTVIY VTHDQIEAMT MADRIVVMNA GRIEQIGASA GALRQAGESF RRELSRFAVD EFFRGRSGER GERRCVEAR
Validated NT Sequence
attttgtnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcag cagtggaact gagcggaatc tacaaacgtt acggcgacac gcaggtcgtg cacggcatcg acctgcatat cgacgacggc gagttcgtcg tgctggtcgg cccgtcgggt tgcggcaaga gcacgctgat gcggatggtc gcgggactcg aggaaatcag cggcggcgaa ctgacgatcg gcggcacgcg cgcgaacggc ttggcgccgc agcagcgcaa tatttcgatg gtgttccaga gctacgcgct gtatccgcat ttgtccgttt acgacaacat cgcgttcggc ccgcgcatcc gcaaggaagc gtcgacgagt ttcaagccgc gcatcgaggc tgccgcgaag atgctgaacc tgagcggtta tctggaccgg ttgccgcgcg cactgtcggg cggtcagcgc cagcgcgtcg cgatggggcg cgcggtggtg cgcgagccgt cgctgttcct gttcgacgaa ccgctctcga atctcgatgc aaagctccgc gtgcagatgc gcacggaaat caaggcgctg catcagcggt tgaagaacac ggtcatctac gtcacgcacg atcagattga agcgatgacg atggccgatc gcatcgtcgt gatgaacgcg gggcgcatcg agcagatcgg ggcgtccgct ggagctttac gacaggccgg cgaatctttt cgtcgcgagc tttctcggtt cgccgtcgat gaatttttcc gcgggcgaag tggtgagcgc ggcgaacggc gttgcgttga agctcggtga cngcgagcag atannntgcc cggcgcaccc gtgacgcccg gcgcgaaant aacgctcggt gtgcggcccg agcacatcga ancatcgact gaangcattc cgatggangt cgaggngatc gagccgannn cgnngaaacn catctgtann nanatcngng nnnnggtgnn tgannanccn nnnnnnggnn ncnatcnagc cnngnnnnnn nnngnnnnnn nnnnccnnnn nnnnncatgc atnnnntcnn nnnnngnnnn nnnnannnng nnnnnaannn nnnnnnannn nnnnntgann nnnnnnnnnn nncnnnnnnn nnanncnnnn nnnannnnnn nnnnnntnnn nnnnnnnnnn nnnnnnaaaa nnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAVELSGI YKRYGDTQVV HGIDLHIDDG EFVVLVGPSG CGKSTLMRMV AGLEEISGGE LTIGGTRANG LAPQQRNISM VFQSYALYPH LSVYDNIAFG PRIRKEASTS FKPRIEAAAK MLNLSGYLDR LPRALSGGQR QRVAMGRAVV REPSLFLFDE PLSNLDAKLR VQMRTEIKAL HQRLKNTVIY VTHDQIEAMT MADRIVVMNA GRIEQIGRPL ELYDRPANLF VASFLGSPSM NFSAGEVVSA ANGVALKLGD GEQIRLPGAP VTPGAKVTLG VRPEHIETST EGIPMDVEVI EPTGAETHLY GKIGGASWCV TTRERASIEP GQRVTVRFPE QHMHLFDTES GRRLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagcagtg gaactgagcg gaatctacaa acgttacggc gacacgcagg tcgtgcacgg catcgacctg catatcgacg acggcgagtt cgtcgtgctg gtcggcccgt cgggttgcgg caagagcacg ctgatgcgga tggtcgcggg actcgaggaa atcagcggcg gcgaactgac gatcggcggc acgcgcgcga acggcttggc gccgcagcag cgcaatattt cgatggtgtt ccagagctac gcgctgtatc cgcatttgtc cgtttacgac aacatcgcgt tcggcccgcg catccgcaag gaagcgtcga cgagtttcaa gccgcgcatc gaggctgccg cgaagatgct gaacctgagc ggttatctgg accggttgcc gcgcgcactg tcgggcggtc agcgccagcg cgtcgcgatg gggcgcgcgg tggtgcgcga gccgtcgctg ttcctgttcg acgaaccgct ctcgaatctc gatgcaaagc tccgcgtgca gatgcgcacg gaaatcaagg cgctgcatca gcggttgaag aacacggtca tctacgtcac gcacgatcag attgaagcga tgacgatggc cgatcgcatc gtcgtgatga acgcggggcg catcgagcag atcgggcgtc cgctggagct ttacgacagg ccggcgaatc ttttcgtcgc gagctttctc ggttcgccgt cgatgaattt ttccgcgggc gaagtggtga gcgcggcgaa cggcgttgcg ttgaagctcg gtgacggcga gcagatacgg ctgcccggcg cacccgtgac gcccggcgcg aaagtaacgc tcggtgtgcg gcccgagcac atcgaaacat cgactgaagg cattccgatg gatgtcgagg tgatcgagcc gacgggcgcg gaaacgcatc tgtacggcaa gatcggcggc gcgtcgtggt gcgtgacgac gcgcgagcgg gcgtcgatcg agcccggtca gcgcgtgacg gtgcgctttc ccgagcagca catgcatctg ttcgatacgg agagtggacg cagattgggc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa