SSGCID: Target Page

BuphA.00057.j

ABC transporter related

CENTER ID:	BuphA.00057.j
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00057.j.A1.GE33052	Full length( BuphA.00057.j )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.3236
RefSeq:	YP_001859263.1
UniProt:	B2JJL9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTVSEQENA SVSVVSGEPA LEIAGLQAWY GESHILHGVD LTVSRGEVVT LLGRNGAGRT TTLRAIMGLT GRRTGSIRVG GRETISMPTH RIAHCGVGYC PEERGIFSSL SCEENLLLPP PVGDKSLMMS IDEIYQMFPN LQERRMSQGT RLSGGEQQML AVARILRTGA NLLLLDEISE GLAPVIVQAL ARMIVTLKAR GYTIVMVEQN FRFAAPLADR FYVMEHGRIV EHFGAKELES KMPVLHDLLG V

NT Sequence

ATGAGCACAG TGAGCGAACA GGAAAACGCA TCAGTCAGTG TCGTGAGCGG CGAGCCCGCG CTGGAGATCG CCGGATTGCA GGCGTGGTAC GGCGAGTCGC ACATTCTGCA TGGCGTCGAT CTGACCGTGA GCCGAGGCGA AGTCGTCACG CTGCTGGGTC GCAATGGCGC GGGCCGCACC ACGACGCTGC GCGCGATCAT GGGCTTGACG GGGCGCCGCA CGGGCTCGAT TCGCGTCGGC GGACGCGAGA CCATATCGAT GCCGACGCAT CGAATTGCCC ATTGCGGCGT CGGCTATTGT CCGGAAGAAC GCGGTATTTT TTCGAGCCTG TCGTGCGAAG AGAATCTGCT GCTGCCGCCG CCCGTCGGCG ACAAGTCGTT GATGATGTCG ATCGACGAGA TTTACCAGAT GTTTCCCAAT CTCCAGGAAC GGCGCATGAG CCAGGGCACG CGGCTGTCAG GCGGCGAGCA GCAGATGCTG GCCGTCGCGC GCATCCTGAG AACGGGTGCG AACCTGCTGT TGCTCGATGA AATCTCGGAA GGTCTCGCGC CTGTGATCGT GCAGGCCCTC GCGCGCATGA TCGTGACGCT GAAAGCCCGC GGCTACACGA TCGTGATGGT CGAACAGAAC TTCCGCTTTG CCGCGCCGCT AGCGGATCGT TTCTATGTGA TGGAGCACGG ACGCATCGTG GAGCATTTCG GTGCAAAGGA ACTCGAAAGC AAAATGCCGG TGCTGCACGA TTTGCTCGGG GTA

Details for BuphA.00057.j.A1.GE33052

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Validated AA Sequence

Validated NT Sequence

ttattatacc ccgagcaaat cgtgcagcac cggcattttg ctttcgagtt cctttgcacc gaaatgctcc acgatgcgtc cgtgctccat cacatagaaa cgatccgcta gcggcgcggc aaagcggaag ttctgttcga ccatcacgat cgtgtagccg cgggctttca gcgtcacgat catgcgcgcg agggcctgca cgatcacagg cgcgagacct tccgagattt catcgagcaa cagcaggttc gcacccgttc tcaggatgcg cgcgacggcc agcatctgct gctcgccgcc tgacagccgc gtgccctggc tcatgcgccg ttcctggaga ttgggaaaca tctggtaaat ctcgtcgatc gacatcatca acgacttgtc gccgacgggc ggcggcagca gcagattctc ttcgcacgac aggctcgaaa aaataccgcg ttcttccgga caatagccga cgccgcaatg ggcaattcga tgcgtcggca tcgatatggt ctcgcgtccg ccgacgcgaa tcgagcccgt gcggcgcccc gtcaagccca tgatcgcgcg cagcgtcgtg gtgcggcccg cgccattgcg acccagcagc gtgacgactt cgcctcggct cacggtcaga tcgacgccat gcagaatgtg cgactcgccg taccacgcct gcaatccggc gatctccagc gcgggctcgc cgctcacgac actgactgat gcgttttcct gttcgctcac tgtgctcatc gaaccaggac cctgggtctg agcttccagg gt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTVSEQEN ASVSVVSGEP ALEIAGLQAW YGESHILHGV DLTVSRGEVV TLLGRNGAGR TTTLRAIMGL TGRRTGSIRV GGRETISMPT HRIAHCGVGY CPEERGIFSS LSCEENLLLP PPVGDKSLMM SIDEIYQMFP NLQERRMSQG TRLSGGEQQM LAVARILRTG ANLLLLDEIS EGLAPVIVQA LARMIVTLKA RGYTIVMVEQ NFRFAAPLAD RFYVMEHGRI VEHFGAKELE SKMPVLHDLL GV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcacagtg agcgaacagg aaaacgcatc agtcagtgtc gtgagcggcg agcccgcgct ggagatcgcc ggattgcagg cgtggtacgg cgagtcgcac attctgcatg gcgtcgatct gaccgtgagc cgaggcgaag tcgtcacgct gctgggtcgc aatggcgcgg gccgcaccac gacgctgcgc gcgatcatgg gcttgacggg gcgccgcacg ggctcgattc gcgtcggcgg acgcgagacc atatcgatgc cgacgcatcg aattgcccat tgcggcgtcg gctattgtcc ggaagaacgc ggtatttttt cgagcctgtc gtgcgaagag aatctgctgc tgccgccgcc cgtcggcgac aagtcgttga tgatgtcgat cgacgagatt taccagatgt ttcccaatct ccaggaacgg cgcatgagcc agggcacgcg gctgtcaggc ggcgagcagc agatgctggc cgtcgcgcgc atcctgagaa cgggtgcgaa cctgctgttg ctcgatgaaa tctcggaagg tctcgcgcct gtgatcgtgc aggccctcgc gcgcatgatc gtgacgctga aagcccgcgg ctacacgatc gtgatggtcg aacagaactt ccgctttgcc gcgccgctag cggatcgttt ctatgtgatg gagcacggac gcatcgtgga gcatttcggt gcaaaggaac tcgaaagcaa aatgccggtg ctgcacgatt tgctcggggt ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa