SSGCID: Target Page

BuphA.00057.i

ABC transporter related

CENTER ID:	BuphA.00057.i
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00057.i.A1.GE33051	Full length( BuphA.00057.i )		1	253

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.3227
RefSeq:	YP_001859256.1
UniProt:	B2JJL2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADHMMTAPI LEVSSLSVRY GKVEALHGAK IAVRPGQIVS VIGPNGAGKS TLLNAIMGAL PITGHAKGGV MYRNENVSHV PIEKRVARGM CLVPEKRELF AGMTVEDNLI LGAYRRKRAG ERDYLDQLEP VFQLFPRLKE RRKQAAGTLS GGERQMLAVG RALMGKPDLL MLDEPSLGLA PLIVKEIFHI ISALRQTGVA TLLIEQNARA ALQISDYGYV LETGELALEG EADALAQNPR VIETYLGLTK KVA

NT Sequence

ATGGCAGATC ACATGATGAC GGCACCGATT CTCGAAGTAA GCAGTCTTTC GGTGCGGTAC GGGAAGGTCG AGGCGTTGCA CGGCGCGAAG ATCGCGGTGC GCCCCGGGCA GATCGTCTCC GTGATTGGTC CGAATGGTGC AGGCAAGTCG ACGTTGCTCA ATGCGATCAT GGGCGCGTTG CCAATAACGG GGCATGCAAA GGGCGGGGTG ATGTATCGCA ACGAGAACGT GAGCCATGTG CCCATCGAGA AGCGTGTCGC GCGCGGCATG TGCCTCGTGC CGGAGAAGCG CGAGTTGTTT GCCGGGATGA CCGTCGAAGA CAACCTGATC CTCGGTGCGT ATCGCCGCAA GCGGGCCGGC GAGCGCGACT ATCTCGACCA GCTCGAACCT GTCTTTCAGC TGTTTCCGCG CCTTAAGGAG CGTCGTAAGC AGGCGGCTGG AACGTTGTCG GGTGGTGAGC GGCAGATGCT GGCTGTTGGC CGGGCACTGA TGGGCAAGCC CGATCTGCTA ATGCTTGACG AGCCGAGCCT TGGCTTGGCG CCATTGATTG TGAAGGAGAT TTTTCACATC ATCAGTGCGC TACGGCAGAC GGGGGTCGCC ACGCTGCTGA TTGAGCAGAA TGCACGCGCC GCACTGCAGA TTTCAGACTA CGGTTATGTA CTCGAAACAG GGGAACTTGC GCTAGAAGGC GAAGCTGACG CACTGGCCCA AAACCCACGC GTGATCGAGA CCTACCTGGG GTTGACGAAG AAAGTCGCG

Details for BuphA.00057.i.A1.GE33051

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMADHMMTAP ILEVSSLSVR YGKVEALHGA KIAVRPGQIV SVIGPNGAGK STLLNAIMGA LPITGHAKGG VMYRNENVSH VPIEKRVARG MCLVPEKREL FAGMTVEDNL ILGAYRRKRA GERDYLDQLE PVFQLFPRLK ERRKQAAGTL SGGERQMLAV GRALMGKPDL LMLDEPSLGL APLIVKEIFH IISALRQTGV ATLLIEQNAR AALXISDYXY VLETXELALE GEADALAQNP RVIETYLGLT KKVA

Validated NT Sequence

ttttntnnnn nttaanangg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcaga tcacatgatg acggcaccga ttctcgaagt aagcagtctt tcggtgcggt acgggaaggt cgaggcgttg cacggcgcga agatcgcggt gcgccccggg cagatcgtct ccgtgattgg tccgaatggt gcaggcaagt cgacgttgct caatgcgatc atgggcgcgt tgccaataac ggggcatgca aagggcgggg tgatgtatcg caacgagaac gtgagccatg tgcccatcga gaagcgtgtc gcgcgcggca tgtgcctcgt gccggagaag cgcgagttgt ttgccgggat gaccgtcgaa gacaacctga tcctcggtgc gtatcgccgc aagcgggccg gcgagcgcga ctatctcgac cagctcgaac ctgtctttca gctgtttccg cgccttaagg agcgtcgtaa gcaggcggct ggaacgttgt cgggtggtga gcggcagatg ctggctgttg gccgggcact gatgggcaag cccgatctgc taatgcttga cgagccgagc cttggcttgg cgccattgat tgtgaaggag atttttcaca tcatcagtgc gctacggcag acgggggtcg ccacgctgct gattgagcag aatgcacgcg ccgcactgca natttcagac tacngttatg tactcgaaac angggaactt gcgctagaag gcgaagctga cgcactggcc caaaacccac gcgtgatcga gacctacctg gggttgacga agaaagtcgc gtaataaaca gcacgaacaa gttctgcagc caagcttctc naggatnccg nntgctaaca aagcccnaaa ggaanctnan ttggctgctg cnnccnntna nnnnnnnncn gcncnnnnnt nccncnnggn nnnncnnnnn nnnnnnnntn ncntnnnnnn nnnnnnntnn ngtnnnnngn nnng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagatcac atgatgacgg caccgattct cgaagtaagc agtctttcgg tgcggtacgg gaaggtcgag gcgttgcacg gcgcgaagat cgcggtgcgc cccgggcaga tcgtctccgt gattggtccg aatggtgcag gcaagtcgac gttgctcaat gcgatcatgg gcgcgttgcc aataacgggg catgcaaagg gcggggtgat gtatcgcaac gagaacgtga gccatgtgcc catcgagaag cgtgtcgcgc gcggcatgtg cctcgtgccg gagaagcgcg agttgtttgc cgggatgacc gtcgaagaca acctgatcct cggtgcgtat cgccgcaagc gggccggcga gcgcgactat ctcgaccagc tcgaacctgt ctttcagctg tttccgcgcc ttaaggagcg tcgtaagcag gcggctggaa cgttgtcggg tggtgagcgg cagatgctgg ctgttggccg ggcactgatg ggcaagcccg atctgctaat gcttgacgag ccgagccttg gcttggcgcc attgattgtg aaggagattt ttcacatcat cagtgcgcta cggcagacgg gggtcgccac gctgctgatt gagcagaatg cacgcgccgc actgcagatt tcagactacg gttatgtact cgaaacaggg gaacttgcgc tagaaggcga agctgacgca ctggcccaaa acccacgcgt gatcgagacc tacctggggt tgacgaagaa agtcgcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa