SSGCID: Target Page

BuphA.00057.h

ABC transporter related

CENTER ID:	BuphA.00057.h
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00057.h.A1.GE33050	Full length( BuphA.00057.h )		1	383

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.2504
RefSeq:	YP_001858574.1
UniProt:	B2JFQ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASISLKGVQ KAYGDNAPVI RNVDLEIGEN EFCVFLGPSG CGKSTLLRMI AGLEDVTDGD LSIGGRIVND VPAAERGVAM VFQSYALFPH MSVYENMAFG LKLAKKPKAE IDRKVREAAR ILQLEALLDR KPRALSGGQR QRVAIGRAIV REPGVFLFDE PLSNLDATLR GQTRIEIARL HKQFATASVV YVTHDQTEAM TLADKIVLLH SGKDTERYGS IAQIGAPLEL YHRPNSRFVA GFIGSPRMNF LPGRVTALDA QGVDVTLDHT NETMRVAVDG STLQGGQPVT LGVRPEHLEL VAHGVSQHDA ILTRTISLIE HLGEHSYVHL EQPGGSVLIA KVPGNARIEQ GDRAVFAAPV RACHLFTEDG FAVKAFDTVE HYI

NT Sequence

ATGGCGAGCA TTTCGTTGAA GGGCGTGCAA AAAGCGTATG GAGACAACGC GCCCGTGATC CGCAACGTCG ATCTGGAGAT CGGCGAAAAC GAGTTTTGCG TGTTCCTCGG TCCCTCGGGC TGCGGCAAGT CCACGTTGCT GCGCATGATC GCAGGGCTGG AGGACGTCAC GGACGGCGAC CTGTCGATTG GCGGTCGCAT CGTCAACGAT GTGCCAGCTG CCGAGCGCGG TGTCGCGATG GTGTTCCAGA GCTACGCGCT GTTTCCGCAC ATGAGCGTGT ACGAGAACAT GGCCTTCGGC CTGAAGCTCG CGAAGAAGCC GAAGGCGGAG ATCGACCGCA AGGTGCGGGA AGCGGCGCGC ATCCTGCAAC TGGAAGCGCT GCTCGATCGC AAGCCGCGCG CGTTGTCGGG CGGGCAGCGG CAGCGTGTTG CGATTGGCCG CGCGATCGTG CGCGAGCCGG GCGTGTTTCT GTTCGACGAA CCCTTGTCCA ATCTCGATGC GACGCTGCGA GGCCAGACGC GCATCGAAAT TGCGCGCCTT CACAAGCAGT TCGCCACGGC GAGCGTCGTC TATGTGACGC ACGACCAGAC GGAGGCGATG ACACTCGCCG ACAAGATCGT GCTGCTGCAC TCGGGCAAGG ACACCGAGCG CTACGGCAGC ATCGCGCAGA TCGGTGCGCC GCTCGAGTTG TACCACCGGC CGAACAGCCG TTTCGTCGCG GGCTTCATCG GCTCGCCACG CATGAACTTC CTGCCGGGCA GGGTGACGGC GCTCGATGCG CAAGGCGTCG ACGTCACGCT CGATCACACG AACGAAACGA TGCGCGTCGC CGTCGACGGC AGCACTTTGC AAGGCGGGCA GCCCGTCACC CTCGGCGTGC GCCCCGAACA TCTCGAACTG GTTGCGCACG GCGTATCACA ACATGATGCA ATCCTCACGC GCACCATTTC GCTGATCGAG CATCTGGGCG AGCACAGCTA CGTGCATCTC GAGCAGCCTG GCGGCAGCGT GCTGATCGCC AAGGTGCCCG GCAATGCGCG TATCGAGCAG GGCGATCGCG CGGTGTTCGC CGCGCCCGTG CGTGCCTGCC ATCTTTTCAC CGAAGACGGA TTCGCCGTGA AGGCGTTCGA TACCGTCGAA CACTACATT

Details for BuphA.00057.h.A1.GE33050

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	83

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMASISLKGV QKAYGDNAPV IRNVDLEIGE NEFCVFLGPS GCGKSTLLRM IAGLEDVTDG DLSIGGRIVN DVPAAERGVA MVFQSYALFP HMSVYENMAF GLKLAKKPKA EIDRKVREAA RILQLEALLD RKPRALSGGQ RQRVAIGRAI VREPGVFLFD EPLSNLDATL RGQTRIEIAR LHKQFATASV VYVTHDQTEA MTLADKIVLL HSGKDTERYG SIAQIGAPLE LYHRPNSRFV AGFIGSPRMN FLPGRVTALD AQGVDVTLDH TNETMRVAVD GSTLQGGQPV TLGVRPEHLE LVAHGVSXXM QSSXAXFR

Validated NT Sequence

attttnttnn ntttaagang gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcga gcatttcgtt gaagggcgtg caaaaagcgt atggagacaa cgcgcccgtg atccgcaacg tcgatctgga gatcggcgaa aacgagtttt gcgtgttcct cggtccctcg ggctgcggca agtccacgtt gctgcgcatg atcgcagggc tggaggacgt cacggacggc gacctgtcga ttggcggtcg catcgtcaac gatgtgccag ctgccgagcg cggtgtcgcg atggtgttcc agagctacgc gctgtttccg cacatgagcg tgtacgagaa catggccttc ggcctgaagc tcgcgaagaa gccgaaggcg gagatcgacc gcaaggtgcg ggaagcggcg cgcatcctgc aactggaagc gctgctcgat cgcaagccgc gcgcgttgtc gggcgggcag cggcagcgtg ttgcgattgg ccgcgcgatc gtgcgcgagc cgggcgtgtt tctgttcgac gaacccttgt ccaatctcga tgcgacgctg cgaggccaga cgcgcatcga aattgcgcgc cttcacaagc agttcgccac ggcgagcgtc gtctatgtga cgcacgacca gacggaggcg atgacactcg ccgacaagat cgtgctgctg cactcgggca aggacaccga gcgctacggc agcatcgcgc agatcggtgc gccgctcgag ttgtaccacc ggccgaacag ccgtttcgtc gcgggcttca tcggctcgcc acgcatgaac ttcctgccgg gcagggtgac ggcgctcgat gcgcaaggcg tcgacgtcac gctcgatcac acgaacgaaa cgatgcgcgt cgccgtcgac ggcagcactt tgcaaggngg gcagcccgtc accctcggcg tgcgccccga acatctcgaa ctggttgcnc acggcgtatc ananntgatg caatcntcan gcgcannntt tcgctgancg agcatctggg cnannncagc tncntgcatc gagcannngg nngcagnnng ntgatnnccn ngnnnncnng nanttatnnn nnnnnnnnnn nnnnnnnntn nncnnnnnnn nnnnnnnnnn cntnnnncna naannnnttn nnnnnnnnnn tnnnnncnnn annnnnanan nnnnnnnnnn nnnnnnnnnc nnnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgagcatt tcgttgaagg gcgtgcaaaa agcgtatgga gacaacgcgc ccgtgatccg caacgtcgat ctggagatcg gcgaaaacga gttttgcgtg ttcctcggtc cctcgggctg cggcaagtcc acgttgctgc gcatgatcgc agggctggag gacgtcacgg acggcgacct gtcgattggc ggtcgcatcg tcaacgatgt gccagctgcc gagcgcggtg tcgcgatggt gttccagagc tacgcgctgt ttccgcacat gagcgtgtac gagaacatgg ccttcggcct gaagctcgcg aagaagccga aggcggagat cgaccgcaag gtgcgggaag cggcgcgcat cctgcaactg gaagcgctgc tcgatcgcaa gccgcgcgcg ttgtcgggcg ggcagcggca gcgtgttgcg attggccgcg cgatcgtgcg cgagccgggc gtgtttctgt tcgacgaacc cttgtccaat ctcgatgcga cgctgcgagg ccagacgcgc atcgaaattg cgcgccttca caagcagttc gccacggcga gcgtcgtcta tgtgacgcac gaccagacgg aggcgatgac actcgccgac aagatcgtgc tgctgcactc gggcaaggac accgagcgct acggcagcat cgcgcagatc ggtgcgccgc tcgagttgta ccaccggccg aacagccgtt tcgtcgcggg cttcatcggc tcgccacgca tgaacttcct gccgggcagg gtgacggcgc tcgatgcgca aggcgtcgac gtcacgctcg atcacacgaa cgaaacgatg cgcgtcgccg tcgacggcag cactttgcaa ggcgggcagc ccgtcaccct cggcgtgcgc cccgaacatc tcgaactggt tgcgcacggc gtatcacaac atgatgcaat cctcacgcgc accatttcgc tgatcgagca tctgggcgag cacagctacg tgcatctcga gcagcctggc ggcagcgtgc tgatcgccaa ggtgcccggc aatgcgcgta tcgagcaggg cgatcgcgcg gtgttcgccg cgcccgtgcg tgcctgccat cttttcaccg aagacggatt cgccgtgaag gcgttcgata ccgtcgaaca ctacatttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa