SSGCID: Target Page

BuphA.00057.f

Urea ABC transporter, ATP-binding protein UrtD

CENTER ID:	BuphA.00057.f
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00057.f.A1.GE33048	Full length( BuphA.00057.f )		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.2400
RefSeq:	YP_001858475.1
UniProt:	B2JF61

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNENPMVPDL ALPEEHDGRA LSGVATMGRV VTPGEIDVSH GAILYLEDVT VSFDGFRALN ALSLSIDVGE LRCIIGPNGA GKTTMMDVIT GKTAPDSGKV FLGQTIDLTR MNEPSIARAG IGRKFQKPTV FEQHPVWENL ELAMKTDKGW MASLRARLDR SAQARIEETL SLIRLEGEAL RPAGELSHGQ KQRLEIGMLL MQQPALVLLD EPAAGMTDDE TMELASLLNR LRGSCSMMVV EHDMEFVAAL AGETGKVTVM AEGRVLAEGS LDEVKRDDAV VESYLGR

NT Sequence

ATGAACGAAA ACCCGATGGT TCCCGATCTT GCTTTACCCG AAGAGCACGA TGGCCGCGCG CTGTCCGGCG TCGCAACGAT GGGCCGCGTC GTCACGCCTG GCGAGATCGA CGTATCGCAC GGGGCGATTC TGTATCTGGA AGACGTGACC GTTTCATTCG ATGGCTTTCG CGCGTTGAAT GCATTGTCGC TGTCGATCGA TGTGGGCGAA CTGCGCTGCA TCATCGGGCC GAACGGCGCG GGCAAGACGA CGATGATGGA CGTAATCACG GGGAAGACCG CGCCTGATTC GGGCAAGGTG TTTCTGGGCC AGACGATCGA CCTGACGCGC ATGAACGAGC CGTCGATTGC CCGTGCAGGC ATAGGCCGCA AGTTTCAGAA GCCGACCGTG TTCGAGCAGC ATCCCGTGTG GGAGAACCTC GAGCTCGCGA TGAAGACGGA CAAGGGCTGG ATGGCTTCGC TGCGAGCCCG GCTGGACCGT TCGGCGCAGG CGCGGATCGA AGAGACCCTG TCCTTGATTC GCCTCGAAGG CGAGGCGTTG CGGCCTGCCG GCGAGTTGTC GCATGGACAG AAGCAGCGAC TCGAGATCGG GATGTTGCTG ATGCAGCAAC CTGCACTTGT GTTGCTCGAT GAGCCTGCAG CCGGCATGAC GGATGATGAG ACGATGGAGT TGGCTTCATT GCTTAACCGG CTTCGCGGGT CTTGCTCGAT GATGGTCGTC GAGCATGATA TGGAGTTTGT GGCCGCTCTG GCGGGTGAAA CCGGCAAGGT GACGGTGATG GCCGAGGGCC GGGTGCTTGC CGAAGGGTCC CTCGATGAGG TCAAGCGGGA CGACGCTGTG GTTGAGAGCT ACCTGGGGAG G

Details for BuphA.00057.f.A1.GE33048

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	33kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNENPMVPD LALPEEHDGR ALSGVATMGR VVTPGEIDVS HGAILYLEDV TVSFDGFRAL NALSLSIDVG ELRCIIGPNG AGKTTMMDVI TGKTAPDSGK VFLGQTIDLT RMNEPSIARA GIGRKFQKPT VFEQHPVWEN LELAMKTDKG WMASLRARLD RSAQARIEET LSLIRLEGEA LRPAGELSHG QKQRLEIGML LMQQPALVLL DEPAAGMTDD ETMELASLLN RLRGSCSMMV VEHDMEFVXA LAGETGKVTV MAEGRVLAEG SLDXVKRDXA VVESYLGRX

Validated NT Sequence

ttttntttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaacga aaacccgatg gttcccgatc ttgctttacc cgaagagcac gatggccgcg cgctgtccgg cgtcgcaacg atgggccgcg tcgtcacgcc tggcgagatc gacgtatcgc acggggcgat tctgtatctg gaagacgtga ccgtttcatt cgatggcttt cgcgcgttga atgcattgtc gctgtcgatc gatgtgggcg aactgcgctg catcatcggg ccgaacggcg cgggcaagac gacgatgatg gacgtaatca cggggaagac cgcgcctgat tcgggcaagg tgtttctggg ccagacgatc gacctgacgc gcatgaacga gccgtcgatt gcccgtgcag gcataggccg caagtttcag aagccgaccg tgttcgagca gcatcccgtg tgggagaacc tcgagctcgc gatgaagacg gacaagggct ggatggcttc gctgcgagcc cggctggacc gttcggcgca ggcgcggatc gaagagaccc tgtccttgat tcgcctcgaa ggcgaggcgt tgcggcctgc cggcgagttg tcgcatggac agaagcagcg actcgagatc gggatgttgc tgatgcagca acctgcactt gtgttgctcg atgagcctgc agccggcatg acggatgatg agacgatgga gttggcttca ttgcttaacc ggcttcgcgg gtcttgctcg atgatggtcg tcgagcatga tatggagttt gtggncgctc tggcgggtga aaccggcaag gtgacngtga tggccgaggg ccgggtgctt gccgaaggnt ccctcgatga ngtcaagcgg gacnacgctg tggttgagag ctacctgggg aggnaataaa cagcacgaac aagttctgca gccaagcttc tcgaggnncc ngctgctaac aaagcccgaa aggaagctga nttggctgct gc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgaaaac ccgatggttc ccgatcttgc tttacccgaa gagcacgatg gccgcgcgct gtccggcgtc gcaacgatgg gccgcgtcgt cacgcctggc gagatcgacg tatcgcacgg ggcgattctg tatctggaag acgtgaccgt ttcattcgat ggctttcgcg cgttgaatgc attgtcgctg tcgatcgatg tgggcgaact gcgctgcatc atcgggccga acggcgcggg caagacgacg atgatggacg taatcacggg gaagaccgcg cctgattcgg gcaaggtgtt tctgggccag acgatcgacc tgacgcgcat gaacgagccg tcgattgccc gtgcaggcat aggccgcaag tttcagaagc cgaccgtgtt cgagcagcat cccgtgtggg agaacctcga gctcgcgatg aagacggaca agggctggat ggcttcgctg cgagcccggc tggaccgttc ggcgcaggcg cggatcgaag agaccctgtc cttgattcgc ctcgaaggcg aggcgttgcg gcctgccggc gagttgtcgc atggacagaa gcagcgactc gagatcggga tgttgctgat gcagcaacct gcacttgtgt tgctcgatga gcctgcagcc ggcatgacgg atgatgagac gatggagttg gcttcattgc ttaaccggct tcgcgggtct tgctcgatga tggtcgtcga gcatgatatg gagtttgtgg ccgctctggc gggtgaaacc ggcaaggtga cggtgatggc cgagggccgg gtgcttgccg aagggtccct cgatgaggtc aagcgggacg acgctgtggt tgagagctac ctggggaggt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa