SSGCID: Target Page

BuphA.00057.e

ABC transporter related

CENTER ID:	BuphA.00057.e
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00057.e.A1.GE33047	Full length( BuphA.00057.e )		1	371

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.664
RefSeq:	YP_001856867.1
UniProt:	B2JEE4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASLSIRDVY KTYPNGVPVL KGVNIDIEDG QFLILVGGSG CGKSTLLNMI AGLETVTKGE IQIGGKTVNN LSPKDRDIAM VFQSYALYPS MTVRDNISFG LNIRKVPKGE QDQIVSRVSK LLQIEHLLDR KPGQLSGGQR QRVAMGRALA RDPAMFLFDE PLSNLDAKLR IEMRSEIKLL HQRLGTTIVY VTHDQIEAMT LGDRIAVMKD GIVQQFGAPQ EIYDSPSNLF VAGFIGAPPM NFIQGKVVEQ GSGVGLELDT GVKRSVLNLP FDGAVKGHVG KEVILGLRPE RITDTRNAHN VEDGKLQPIE VKVDVIEPTG PDTLVFAQVN GKRIVSRVHP GANPQPDQNM SLMFDVSKAV LFDPKSEERI A

NT Sequence

ATGGCAAGCC TTTCCATCCG TGACGTGTAC AAGACCTACC CGAACGGGGT GCCTGTCCTG AAGGGTGTCA ACATTGACAT CGAAGACGGT CAGTTCCTGA TTCTCGTCGG CGGCTCGGGC TGCGGTAAGT CGACGCTGCT TAACATGATC GCCGGTCTCG AGACCGTGAC GAAGGGCGAG ATTCAGATCG GCGGCAAGAC GGTGAACAAC CTGTCGCCGA AGGATCGCGA TATCGCGATG GTGTTCCAGT CGTATGCGCT GTATCCGTCG ATGACGGTGC GCGACAACAT CTCTTTTGGC TTGAACATTC GCAAGGTGCC GAAGGGTGAG CAGGATCAGA TCGTTTCGCG CGTGTCGAAG CTGCTGCAGA TCGAGCATCT ACTGGATCGC AAGCCGGGCC AGCTTTCCGG TGGTCAGCGT CAGCGCGTGG CGATGGGGCG TGCGTTGGCG CGCGATCCGG CGATGTTCCT GTTCGATGAG CCTTTGTCGA ACCTGGATGC GAAGCTGCGG ATCGAGATGC GCTCGGAGAT CAAGCTGCTG CATCAGCGCC TGGGCACGAC GATTGTTTAT GTGACGCATG ATCAGATCGA GGCCATGACG CTGGGTGATC GTATCGCCGT GATGAAGGAC GGAATCGTTC AGCAGTTCGG TGCGCCGCAA GAGATCTATG ACTCGCCTTC GAATCTGTTC GTCGCTGGGT TCATCGGTGC ACCGCCGATG AACTTCATTC AGGGGAAGGT TGTTGAGCAG GGTTCGGGCG TTGGGCTCGA GTTGGATACT GGCGTTAAGC GCTCGGTGCT GAATCTGCCG TTCGATGGCG CGGTGAAGGG ACATGTCGGC AAGGAAGTGA TTCTTGGCCT GCGTCCTGAA CGCATCACCG ATACGCGTAA TGCTCACAAC GTCGAGGACG GTAAGCTGCA GCCGATCGAA GTCAAGGTCG ATGTGATCGA GCCGACTGGC CCTGACACCC TGGTGTTCGC GCAAGTGAAT GGCAAGCGAA TCGTCAGCCG GGTTCACCCG GGCGCTAATC CGCAACCGGA TCAGAACATG TCGCTGATGT TCGATGTTTC CAAGGCAGTT CTGTTCGATC CAAAGTCGGA AGAGCGAATT GCT

Details for BuphA.00057.e.A1.GE33047

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMASLSIRDV YKTYPNGVPV LKGVNIDIED GQFLILVGGS GCGKSTLLNM IAGLETVTKG EIQIGGKTVN NLSPKDRDIA MVFQSYALYP SMTVRDNISF GLNIRKVPKG EQDQIVSRVS KLLQIEHLLD RKPGQLSGGQ RQRVAMGRAL ARDPAMFLFD EPLSNLDAKL RIEMRSEIKL LHQRLGTTIV YVTHDQIEAM TLGDRIAVMK DGIVQQFGAP QEIYDSPSNL FVAGFIGAPP MNFIQGKVLS XVRALGSSWI LALSARC

Validated NT Sequence

attttgtttn nctttaagan ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggca agcctttcca tccgtgacgt gtacaagacc tacccgaacg gggtgcctgt cctgaagggt gtcaacattg acatcgaaga cggtcagttc ctgattctcg tcggcggctc gggctgcggt aagtcgacgc tgcttaacat gatcgccggt ctcgagaccg tgacgaaggg cgagattcag atcggcggca agacggtgaa caacctgtcg ccgaaggatc gcgatatcgc gatggtgttc cagtcgtatg cgctgtatcc gtcgatgacg gtgcgcgaca acatctcttt tggcttgaac attcgcaagg tgccgaaggg tgagcaggat cagatcgttt cgcgcgtgtc gaagctgctg cagatcgagc atctactgga tcgcaagccg ggccagcttt ccggtggtca gcgtcagcgc gtggcgatgg ggcgtgcgtt ggcgcgcgat ccggcgatgt tcctgttcga tgagcctttg tcgaacctgg atgcgaagct gcggatcgag atgcgctcgg agatcaagct gctgcatcag cgcctgggca cgacgattgt ttatgtgacg catgatcaga tcgaggccat gacgctgggt gatcgtatcg ccgtgatgaa ggacggaatc gttcagcagt tcggtgcgcc gcaagagatc tatgactcgc cttcgaatct gttcgtcgct gggttcatcg gtgcaccgcc gatgaacttc attcagggga aggtgttgag canggttcgg gcgttgggct cgagttggat actggcgtta agcgctcggt gctgaatctg ccgttcgatg gcgcggtgna aggnacatgt cggcaaggaa gtgantcttg gnctgcgtcc tgaacgcatc accgatacgc gtaatgctca cnacgtcnag ganggtaagc tgcagccnat cgaagtcaag gncgangtga tcganccnac tggnccngan nncctggnnn tcnnnncant gaatggnaag cgaatcgtca ncnngntnnn cccnnnnntn annccnnnnn cngatcnnna anatgnnnnc nnnngntnnn nntttncnnn gnnnnnnnnn ntnancnnnn nngnannnng nntnnnntnn nnnannnnnn nnanantnnn gnnnnnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaagcctt tccatccgtg acgtgtacaa gacctacccg aacggggtgc ctgtcctgaa gggtgtcaac attgacatcg aagacggtca gttcctgatt ctcgtcggcg gctcgggctg cggtaagtcg acgctgctta acatgatcgc cggtctcgag accgtgacga agggcgagat tcagatcggc ggcaagacgg tgaacaacct gtcgccgaag gatcgcgata tcgcgatggt gttccagtcg tatgcgctgt atccgtcgat gacggtgcgc gacaacatct cttttggctt gaacattcgc aaggtgccga agggtgagca ggatcagatc gtttcgcgcg tgtcgaagct gctgcagatc gagcatctac tggatcgcaa gccgggccag ctttccggtg gtcagcgtca gcgcgtggcg atggggcgtg cgttggcgcg cgatccggcg atgttcctgt tcgatgagcc tttgtcgaac ctggatgcga agctgcggat cgagatgcgc tcggagatca agctgctgca tcagcgcctg ggcacgacga ttgtttatgt gacgcatgat cagatcgagg ccatgacgct gggtgatcgt atcgccgtga tgaaggacgg aatcgttcag cagttcggtg cgccgcaaga gatctatgac tcgccttcga atctgttcgt cgctgggttc atcggtgcac cgccgatgaa cttcattcag gggaaggttg ttgagcaggg ttcgggcgtt gggctcgagt tggatactgg cgttaagcgc tcggtgctga atctgccgtt cgatggcgcg gtgaagggac atgtcggcaa ggaagtgatt cttggcctgc gtcctgaacg catcaccgat acgcgtaatg ctcacaacgt cgaggacggt aagctgcagc cgatcgaagt caaggtcgat gtgatcgagc cgactggccc tgacaccctg gtgttcgcgc aagtgaatgg caagcgaatc gtcagccggg ttcacccggg cgctaatccg caaccggatc agaacatgtc gctgatgttc gatgtttcca aggcagttct gttcgatcca aagtcggaag agcgaattgc ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa