BuphA.00057.b
ABC transporter related
| CENTER ID: | BuphA.00057.b |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00057.b.A1.GE33044 | Full length( BuphA.00057.b ) | 1 | 260 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00057.b.A1.PS01348 | Full length( BuphA.00057.b ) | 1 | 260 |
Structures
4WBS
DEPOSITED: 9/3/2014
DETERMINATION: XRay
CLONE: BuphA.00057.b.A1.GE33044
PROTEIN: BuphA.00057.b.A1.PS01348
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_132803 |
| PATRIC ID: | fig|391038.7.peg.341 |
| RefSeq: | YP_001856566.1 |
| UniProt: | B2JCQ1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAPALPNRK PAGTSSSLVV RNLKKRYGSR TVVKDVSLDV KSGEVVGLLG PNGAGKTTSF YMIVGLVPLD AGEIDLDGKS ISLLPIHKRA SLGLSYLPQE ASVFRKLSVE ENIRAVLELQ VGDDGKRLSK DAIASRTEAL LDELQISHLR ENPALSLSGG ERRRVEIARA LATNPSFILL DEPFAGVDPI AVLEIQKIVK FLKQRNIGVL ITDHNVRETL GICDHAYIIS DGSVLAAGAP GDIIENESVR RVYLGEHFRM
NT Sequence
GTGAGCGCCC CAGCCCTTCC CAACCGAAAG CCGGCCGGCA CCAGCAGTTC GCTCGTCGTT CGCAATCTGA AAAAGCGTTA TGGCTCGCGT ACGGTCGTCA AGGACGTCTC GCTCGACGTG AAAAGCGGCG AAGTGGTCGG CCTGCTCGGT CCGAACGGCG CGGGCAAGAC GACGTCGTTC TACATGATCG TCGGCCTCGT GCCGCTCGAC GCCGGCGAGA TCGATCTCGA CGGCAAGTCG ATCAGCCTTC TGCCCATTCA CAAGCGCGCG TCCCTCGGCC TGTCGTATCT GCCGCAAGAA GCGTCCGTGT TCCGCAAGCT TTCCGTCGAG GAAAACATTC GCGCGGTGCT CGAACTGCAG GTCGGCGACG ACGGCAAGCG CCTGTCGAAA GACGCGATCG CGTCGCGCAC GGAAGCGCTG CTCGACGAAC TGCAGATCTC GCATCTGCGC GAAAACCCCG CGCTGTCGCT GTCGGGCGGC GAACGCCGCC GCGTCGAGAT CGCGCGCGCG CTCGCGACCA ACCCGAGCTT TATCCTGCTA GACGAACCGT TCGCGGGCGT CGACCCGATC GCCGTTCTTG AAATCCAGAA GATCGTGAAG TTCCTGAAGC AGCGCAACAT CGGCGTGCTC ATCACGGACC ACAACGTCCG TGAAACGCTC GGTATCTGCG ATCACGCATA TATCATCAGC GATGGCAGCG TGCTCGCAGC AGGCGCGCCC GGCGACATCA TTGAAAACGA GAGCGTGCGC CGCGTCTATC TGGGCGAACA TTTCCGCATG
Details for BuphA.00057.b.A1.GE33044
| HARVESTED ON: | 8/16/2011 |
| SEQUENCED ON: | 8/22/2011 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAPALPN RKPAGTSSSL VVRNLKKRYG SRTVVKDVSL DVKSGEVVGL LGPNGAGKTT SFYMIVGLVP LDAGEIDLDG KSISLLPIHK RASLGLSYLP QEASVFRKLS VEENIRAVLE LQVGDDGKRL SKDAIASRTE ALLDELQISH LRENPALSLS GGERRRVEIA RALATNPSFI LLDEPFAGVD PIAVLEIQKI VKFLKQRNIG VLITDHNVRE TLGICDHAYI ISDGSVLAAG APGDIIENES VRRVYLGEHF RM
Validated NT Sequence
ttattacatg cggaaatgtt cgcccagata gacgcggcgc acgctctcgt tttcaatgat gtcgccgggc gcgcctgctg cgagcacgct gccatcgctg atgatatatg cgtgatcgca gataccgagc gtttcacgga cgttgtggtc cgtgatgagc acgccgatgt tgcgctgctt caggaacttc acgatcttct ggatttcaag aacggcgatc gggtcgacgc ccgcgaacgg ttcgtctagc aggataaagc tcgggttggt cgcgagcgcg cgcgcgatct cgacgcggcg gcgttcgccg cccgacagcg acagcgcggg gttttcgcgc agatgcgaga tctgcagttc gtcgagcagc gcttccgtgc gcgacgcgat cgcgtctttc gacaggcgct tgccgtcgtc gccgacctgc agttcgagca ccgcgcgaat gttttcctcg acggaaagct tgcggaacac ggacgcttct tgcggcagat acgacaggcc gagggacgcg cgcttgtgaa tgggcagaag gctgatcgac ttgccgtcga gatcgatctc gccggcgtcg agcggcacga ggccgacgat catgtagaac gacgtcgtct tgcccgcgcc gttcggaccg agcaggccga ccacttcgcc gcttttcacg tcgagcgaga cgtccttgac gaccgtacgc gagccataac gctttttcag attgcgaacg acgagcgaac tgctggtgcc ggccggcttt cggttgggaa gggctggggc gctcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAPALPN RKPAGTSSSL VVRNLKKRYG SRTVVKDVSL DVKSGEVVGL LGPNGAGKTT SFYMIVGLVP LDAGEIDLDG KSISLLPIHK RASLGLSYLP QEASVFRKLS VEENIRAVLE LQVGDDGKRL SKDAIASRTE ALLDELQISH LRENPALSLS GGERRRVEIA RALATNPSFI LLDEPFAGVD PIAVLEIQKI VKFLKQRNIG VLITDHNVRE TLGICDHAYI ISDGSVLAAG APGDIIENES VRRVYLGEHF RM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgcc ccagcccttc ccaaccgaaa gccggccggc accagcagtt cgctcgtcgt tcgcaatctg aaaaagcgtt atggctcgcg tacggtcgtc aaggacgtct cgctcgacgt gaaaagcggc gaagtggtcg gcctgctcgg tccgaacggc gcgggcaaga cgacgtcgtt ctacatgatc gtcggcctcg tgccgctcga cgccggcgag atcgatctcg acggcaagtc gatcagcctt ctgcccattc acaagcgcgc gtccctcggc ctgtcgtatc tgccgcaaga agcgtccgtg ttccgcaagc tttccgtcga ggaaaacatt cgcgcggtgc tcgaactgca ggtcggcgac gacggcaagc gcctgtcgaa agacgcgatc gcgtcgcgca cggaagcgct gctcgacgaa ctgcagatct cgcatctgcg cgaaaacccc gcgctgtcgc tgtcgggcgg cgaacgccgc cgcgtcgaga tcgcgcgcgc gctcgcgacc aacccgagct ttatcctgct agacgaaccg ttcgcgggcg tcgacccgat cgccgttctt gaaatccaga agatcgtgaa gttcctgaag cagcgcaaca tcggcgtgct catcacggac cacaacgtcc gtgaaacgct cggtatctgc gatcacgcat atatcatcag cgatggcagc gtgctcgcag caggcgcgcc cggcgacatc attgaaaacg agagcgtgcg ccgcgtctat ctgggcgaac atttccgcat gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BuphA.00057.b.A1.PS01348
| PURIFICATION DATe: | 1/25/2012 |
| CONCENTRATION: | 54.06mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAPALPN RKPAGTSSSL VVRNLKKRYG SRTVVKDVSL DVKSGEVVGL LGPNGAGKTT SFYMIVGLVP LDAGEIDLDG KSISLLPIHK RASLGLSYLP QEASVFRKLS VEENIRAVLE LQVGDDGKRL SKDAIASRTE ALLDELQISH LRENPALSLS GGERRRVEIA RALATNPSFI LLDEPFAGVD PIAVLEIQKI VKFLKQRNIG VLITDHNVRE TLGICDHAYI ISDGSVLAAG APGDIIENES VRRVYLGEHF RM
Validated NT Sequence
ttattacatg cggaaatgtt cgcccagata gacgcggcgc acgctctcgt tttcaatgat gtcgccgggc gcgcctgctg cgagcacgct gccatcgctg atgatatatg cgtgatcgca gataccgagc gtttcacgga cgttgtggtc cgtgatgagc acgccgatgt tgcgctgctt caggaacttc acgatcttct ggatttcaag aacggcgatc gggtcgacgc ccgcgaacgg ttcgtctagc aggataaagc tcgggttggt cgcgagcgcg cgcgcgatct cgacgcggcg gcgttcgccg cccgacagcg acagcgcggg gttttcgcgc agatgcgaga tctgcagttc gtcgagcagc gcttccgtgc gcgacgcgat cgcgtctttc gacaggcgct tgccgtcgtc gccgacctgc agttcgagca ccgcgcgaat gttttcctcg acggaaagct tgcggaacac ggacgcttct tgcggcagat acgacaggcc gagggacgcg cgcttgtgaa tgggcagaag gctgatcgac ttgccgtcga gatcgatctc gccggcgtcg agcggcacga ggccgacgat catgtagaac gacgtcgtct tgcccgcgcc gttcggaccg agcaggccga ccacttcgcc gcttttcacg tcgagcgaga cgtccttgac gaccgtacgc gagccataac gctttttcag attgcgaacg acgagcgaac tgctggtgcc ggccggcttt cggttgggaa gggctggggc gctcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAPALPN RKPAGTSSSL VVRNLKKRYG SRTVVKDVSL DVKSGEVVGL LGPNGAGKTT SFYMIVGLVP LDAGEIDLDG KSISLLPIHK RASLGLSYLP QEASVFRKLS VEENIRAVLE LQVGDDGKRL SKDAIASRTE ALLDELQISH LRENPALSLS GGERRRVEIA RALATNPSFI LLDEPFAGVD PIAVLEIQKI VKFLKQRNIG VLITDHNVRE TLGICDHAYI ISDGSVLAAG APGDIIENES VRRVYLGEHF RM
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCGCC CCAGCCCTTC CCAACCGAAA GCCGGCCGGC ACCAGCAGTT CGCTCGTCGT TCGCAATCTG AAAAAGCGTT ATGGCTCGCG TACGGTCGTC AAGGACGTCT CGCTCGACGT GAAAAGCGGC GAAGTGGTCG GCCTGCTCGG TCCGAACGGC GCGGGCAAGA CGACGTCGTT CTACATGATC GTCGGCCTCG TGCCGCTCGA CGCCGGCGAG ATCGATCTCG ACGGCAAGTC GATCAGCCTT CTGCCCATTC ACAAGCGCGC GTCCCTCGGC CTGTCGTATC TGCCGCAAGA AGCGTCCGTG TTCCGCAAGC TTTCCGTCGA GGAAAACATT CGCGCGGTGC TCGAACTGCA GGTCGGCGAC GACGGCAAGC GCCTGTCGAA AGACGCGATC GCGTCGCGCA CGGAAGCGCT GCTCGACGAA CTGCAGATCT CGCATCTGCG CGAAAACCCC GCGCTGTCGC TGTCGGGCGG CGAACGCCGC CGCGTCGAGA TCGCGCGCGC GCTCGCGACC AACCCGAGCT TTATCCTGCT AGACGAACCG TTCGCGGGCG TCGACCCGAT CGCCGTTCTT GAAATCCAGA AGATCGTGAA GTTCCTGAAG CAGCGCAACA TCGGCGTGCT CATCACGGAC CACAACGTCC GTGAAACGCT CGGTATCTGC GATCACGCAT ATATCATCAG CGATGGCAGC GTGCTCGCAG CAGGCGCGCC CGGCGACATC ATTGAAAACG AGAGCGTGCG CCGCGTCTAT CTGGGCGAAC ATTTCCGCAT GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA