BuphA.00057.a

Spermidine/putrescine ABC transporter ATPase subunit

CENTER ID: BuphA.00057.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00057.a.A1.GE33043 Full length( BuphA.00057.a ) 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.243
RefSeq: YP_001856463.1
UniProt: B2JC53

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAFLEIDSLH KAFGANTALH HFDMKIERGE FITFLGPSGC GKTTVLRMIA GFETPTRGTI RLDGRDVTHL RTRQRKVGMV FQAYALFPNM TVAQNIGFGL KIANRPQSEI KQRVDEMLQL IKLPHLGERY PWQLSGGQQQ RVALARALAG KPQVLLLDEP LSALDAKIRI SLRQDIRALQ RELGITSIFV THDQEEALSI SDRIVVMNEG RVEQVGTPLE IYNYPRTRFV ASFVGTLNIL SGHVIDPSSG RMAVDGQELH TTQALPADDA GKKRMLAVRP EAIVLEPPAA GRNTLTATVE EVSFLGAIVR IRTRVKDEVV SLDIFNDPNR RLPERGQPVA LGFSHENLLV LEEGAQAQG
NT Sequence
ATGGCATTTC TTGAAATCGA CAGTCTGCAC AAGGCGTTCG GCGCAAACAC GGCGCTGCAT CACTTCGACA TGAAGATCGA GCGCGGCGAG TTCATCACGT TTCTCGGGCC GTCGGGGTGC GGCAAGACGA CCGTGCTGCG CATGATCGCC GGCTTCGAAA CGCCGACGCG CGGCACGATC CGCCTCGACG GGCGCGACGT CACGCATCTG CGCACGCGAC AGCGCAAGGT CGGCATGGTG TTTCAGGCGT ATGCGCTGTT TCCGAACATG ACGGTCGCGC AGAACATCGG CTTTGGGCTG AAGATCGCGA ACCGGCCGCA GAGCGAGATC AAACAGCGCG TCGACGAAAT GCTGCAACTG ATCAAGCTGC CGCACCTGGG CGAACGGTAT CCGTGGCAGC TGTCGGGCGG CCAGCAGCAG CGTGTCGCGC TGGCGCGCGC GCTCGCTGGC AAGCCGCAAG TGCTGCTGCT CGACGAGCCG CTGTCGGCAC TCGACGCGAA GATCCGCATT TCGCTGCGTC AGGACATTCG CGCGTTGCAG CGCGAACTCG GCATCACGTC GATCTTCGTC ACGCACGATC AGGAAGAGGC GCTGTCGATT TCCGACCGCA TCGTCGTGAT GAACGAAGGG CGCGTCGAGC AGGTCGGCAC GCCGCTTGAG ATTTACAACT ATCCGCGCAC GCGTTTCGTC GCGTCGTTCG TCGGCACGCT GAATATCCTG TCGGGCCATG TGATCGACCC GTCGTCGGGA CGCATGGCCG TCGACGGTCA GGAACTGCAC ACCACGCAAG CCTTGCCCGC CGACGACGCC GGCAAGAAGC GCATGCTTGC CGTGCGACCC GAGGCGATCG TGCTGGAGCC GCCCGCTGCG GGCCGCAACA CGCTGACGGC GACGGTCGAA GAGGTGAGCT TCCTCGGCGC GATCGTGCGC ATCCGCACGC GCGTGAAAGA CGAGGTCGTG TCGCTCGATA TTTTCAACGA CCCGAATCGC CGCTTGCCCG AACGCGGCCA GCCGGTGGCG CTGGGTTTCT CGCACGAGAA TCTGCTGGTT CTCGAAGAGG GTGCGCAGGC CCAGGGT
Details for BuphA.00057.a.A1.GE33043
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 109
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFLEIDSL HKAFGANTAL HHFDMKIERG EFITFLGPSG CGKTTVLRMI AGFETPTRGT IRLDGRDVTH LRTRQRKVGM VFQAYALFPN MTVAQNIGFG LKIANRPQSE IKQRVDEMLQ LIKLPHLGER YPWQLSGGQQ QRVALARALA GKPQVLLLDE PLSALDAKIR ISLRQDIRAL QRELGITSIF VTHDQEEALS ISDRIVVMNE GRVEQVGTPL EIYNYPRTRF VASFVGTLNI LSGHVIDPSS GRMAVDGQEL HTTQALPADX AXKKRMLAVR PEAIVLEPPA AGRNTLTXXV EXVSFLGAIV RIRXXXKXXV VSXRXFNDPN XXXXXXXXXX XWXXXXXXXX XXXXXXXXXX XXXXXPXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
attttgntnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcat ttcttgaaat cgacagtctg cacaaggcgt tcggcgcaaa cacggcgctg catcacttcg acatgaagat cgagcgcggc gagttcatca cgtttctcgg gccgtcgggg tgcggcaaga cgaccgtgct gcgcatgatc gccggcttcg aaacgccgac gcgcggcacg atccgcctcg acgggcgcga cgtcacgcat ctgcgcacgc gacagcgcaa ggtcggcatg gtgtttcagg cgtatgcgct gtttccgaac atgacggtcg cgcagaacat cggctttggg ctgaagatcg cgaaccggcc gcagagcgag atcaaacagc gcgtcgacga aatgctgcaa ctgatcaagc tgccgcacct gggcgaacgg tatccgtggc agctgtcggg cggccagcag cagcgtgtcg cgctggcgcg cgcgctcgct ggcaagccgc aagtgctgct gctcgacgag ccgctgtcgg cactcgacgc gaagatccgc atttcgctgc gtcaggacat tcgcgcgttg cagcgcgaac tcggcatcac gtcgatcttc gtcacgcacg atcaggaaga ggcgctgtcg atttccgacc gcatcgtcgt gatgaacgaa gggcgcgtcg agcaggtcgg cacgccgctt gagatttaca actatccgcg cacgcgtttc gtcgcgtcgt tcgtcggcac gctgaatatc ctgtcgggcc atgtgatcga cccgtcgtcg ggacgcatgg ccgtcgacgg tcaggaactg cacaccacgc aagccttgcc cgccgacnac gccnnnaaga agcgcatgct tgccgtgcga cccgaggcga tcgtgctgga gccgcccgct gcgggccgca acacgctgac gncganggtc gaagangtga gcttcctcgg cgcgatcgtg cgcatccgcn cgcncgngaa anangangtc gtgtcnnntc gnnntttcaa cgacccnaat ncnncttgnc ngaanncngn nancngnnng nnctgggntn nnncanntgn nngntnnnnn nanangnngn nnnggcnnng gnnnnnnann nnnnantnnn nnnnnccnan nnnnnnnnnn nncnnnnatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naannnnnnn nnnnccnnnn ncncnnncc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFLEIDSL HKAFGANTAL HHFDMKIERG EFITFLGPSG CGKTTVLRMI AGFETPTRGT IRLDGRDVTH LRTRQRKVGM VFQAYALFPN MTVAQNIGFG LKIANRPQSE IKQRVDEMLQ LIKLPHLGER YPWQLSGGQQ QRVALARALA GKPQVLLLDE PLSALDAKIR ISLRQDIRAL QRELGITSIF VTHDQEEALS ISDRIVVMNE GRVEQVGTPL EIYNYPRTRF VASFVGTLNI LSGHVIDPSS GRMAVDGQEL HTTQALPADD AGKKRMLAVR PEAIVLEPPA AGRNTLTATV EEVSFLGAIV RIRTRVKDEV VSLDIFNDPN RRLPERGQPV ALGFSHENLL VLEEGAQAQG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatttctt gaaatcgaca gtctgcacaa ggcgttcggc gcaaacacgg cgctgcatca cttcgacatg aagatcgagc gcggcgagtt catcacgttt ctcgggccgt cggggtgcgg caagacgacc gtgctgcgca tgatcgccgg cttcgaaacg ccgacgcgcg gcacgatccg cctcgacggg cgcgacgtca cgcatctgcg cacgcgacag cgcaaggtcg gcatggtgtt tcaggcgtat gcgctgtttc cgaacatgac ggtcgcgcag aacatcggct ttgggctgaa gatcgcgaac cggccgcaga gcgagatcaa acagcgcgtc gacgaaatgc tgcaactgat caagctgccg cacctgggcg aacggtatcc gtggcagctg tcgggcggcc agcagcagcg tgtcgcgctg gcgcgcgcgc tcgctggcaa gccgcaagtg ctgctgctcg acgagccgct gtcggcactc gacgcgaaga tccgcatttc gctgcgtcag gacattcgcg cgttgcagcg cgaactcggc atcacgtcga tcttcgtcac gcacgatcag gaagaggcgc tgtcgatttc cgaccgcatc gtcgtgatga acgaagggcg cgtcgagcag gtcggcacgc cgcttgagat ttacaactat ccgcgcacgc gtttcgtcgc gtcgttcgtc ggcacgctga atatcctgtc gggccatgtg atcgacccgt cgtcgggacg catggccgtc gacggtcagg aactgcacac cacgcaagcc ttgcccgccg acgacgccgg caagaagcgc atgcttgccg tgcgacccga ggcgatcgtg ctggagccgc ccgctgcggg ccgcaacacg ctgacggcga cggtcgaaga ggtgagcttc ctcggcgcga tcgtgcgcat ccgcacgcgc gtgaaagacg aggtcgtgtc gctcgatatt ttcaacgacc cgaatcgccg cttgcccgaa cgcggccagc cggtggcgct gggtttctcg cacgagaatc tgctggttct cgaagagggt gcgcaggccc agggttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa