BuphA.00046.c

Alpha,alpha-trehalose-phosphate synthase (UDP-forming)

CENTER ID: BuphA.00046.c
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00046.c.B1.GE40109 Full length( BuphA.00046.c ) 1 479
BuphA.00046.c.B11.GE40110 Full length( BuphA.00046.c ) 1 479
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00046.c.B11.PS37993 Full length( BuphA.00046.c ) 1 479
BuphA.00046.c.B11.PS37994 Full length( BuphA.00046.c ) 1 479
BuphA.00046.c.B1.PS38009 Full length( BuphA.00046.c ) 1 479

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.1107
RefSeq: YP_001857283.1
UniProt: B2JGQ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRLIVVSNR VATPTETRGS AGGLAVGVFG ALKDAGGVWF GWSGDVVSET VANAGPTLET DGSVTFATTG LTRKDYDQYY RGFSNATLWP VFHYRNDLAR YEREEYAGYR RVNAWLSHKL IKLLEPDDII WVHDYHLIPF AEALRSEGVR NRIGFFLHIP FPAPQILLNI PPHEELVKSL CCYDLLGFQT ENDELAFHDY LRRHARGTVS ADGHAQAFGR QMRTGVYRIG VFPDEIAEQA KRYESRQHVL DLKQSLEGRK LIMSVDRLDY SKGLVERFRA FEALLERSPE WRGNVTLVQI APPTRSDVTT YQDIRQQLEY EAGRINGKYS GLDYTPIRYL NQQYDRWKLM SLFRESQVGF VTPLHDGMNL VAKEYVAAQN PDDPGVLVLS MFAGAAAELE GALIVNPHDS IGMCEALQRA LAMPLDERKR RHDIDMQALR KNDLGVWRDS FLRDLRSVPL AGSPSDASGT STDSAKRTK
NT Sequence
ATGGGCCGAC TGATCGTCGT GTCGAATCGC GTGGCAACGC CGACTGAAAC GAGAGGCTCG GCGGGCGGGC TGGCCGTCGG CGTGTTCGGC GCGCTGAAGG ATGCGGGCGG GGTGTGGTTC GGGTGGAGCG GCGACGTCGT CAGCGAGACG GTGGCGAACG CCGGGCCCAC GCTCGAAACG GACGGCAGCG TCACGTTTGC CACCACAGGC CTCACGCGCA AGGACTACGA CCAGTACTAC CGGGGCTTTT CGAACGCGAC GCTGTGGCCC GTCTTCCACT ATCGCAACGA TCTCGCTCGC TACGAACGCG AGGAATACGC AGGCTACCGG CGGGTCAATG CGTGGCTCTC GCACAAGCTC ATCAAGCTGC TGGAGCCTGA CGACATTATC TGGGTCCACG ACTATCACCT CATCCCCTTC GCGGAAGCGC TGCGATCCGA GGGCGTCAGG AATCGCATCG GTTTTTTCCT GCACATTCCG TTTCCGGCGC CGCAGATCCT GTTGAACATT CCGCCGCATG AAGAACTGGT GAAGTCGTTA TGTTGCTACG ACCTGCTCGG TTTTCAGACC GAAAACGACG AGCTCGCGTT TCATGACTAT CTGCGCCGGC ACGCACGCGG CACCGTCTCG GCTGACGGAC ACGCGCAAGC GTTCGGTCGG CAGATGCGCA CGGGCGTCTA CCGGATCGGC GTGTTCCCCG ACGAGATTGC CGAGCAGGCA AAGCGCTATG AGAGCCGTCA GCACGTGCTC GACCTCAAGC AGAGCCTGGA AGGGCGCAAG CTGATCATGA GCGTCGACCG TCTCGACTAT TCGAAGGGGC TGGTCGAGCG CTTCCGCGCA TTCGAGGCGC TGCTGGAGCG CTCGCCCGAA TGGCGCGGCA ATGTCACACT TGTGCAGATT GCGCCGCCCA CGCGCTCCGA CGTCACGACG TACCAGGACA TCCGTCAGCA ACTCGAATAC GAAGCCGGGC GTATCAACGG AAAATACTCA GGCCTGGACT ACACGCCCAT TCGTTATTTG AATCAGCAAT ACGACCGCTG GAAGCTGATG TCGCTGTTCC GCGAGTCGCA GGTCGGATTC GTCACGCCCT TGCACGACGG GATGAATCTC GTCGCGAAGG AATATGTCGC CGCCCAGAAC CCGGACGATC CAGGTGTGCT CGTACTGTCG ATGTTCGCGG GCGCGGCCGC CGAACTGGAA GGGGCATTGA TCGTGAATCC TCACGATTCG ATCGGCATGT GCGAAGCGTT GCAGCGTGCG CTCGCGATGC CGCTCGACGA ACGCAAGCGG CGTCACGATA TCGACATGCA GGCGCTGCGC AAGAACGATC TCGGTGTGTG GCGCGACTCG TTCCTGCGCG ATCTGCGCAG CGTGCCGCTT GCCGGGTCGC CGAGCGACGC TTCCGGAACC TCGACAGACT CAGCAAAAAG GACAAAG
Details for BuphA.00046.c.B1.GE40109
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG RLIVVSNRVA TPTETRGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLETDG SVTFATTGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYAGYRRV NAWLSHKLIK LLEPDDIIWV HDYHLIPFAE ALRSEGVRNR IGFFLHIPFP APQILLNIPP HEELVKSLCC YDLLGFQTEN DELAFHDYLR RHARGTVSAD GHAQAFGRQM RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE ALLERSPEWR GNVTLVQIAP PTRSDVTTYQ DIRQQLEYEA GRINGKYSGL DYTPIRYLNQ QYDRWKLMSL FRESQVGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSMF AGAAAELEGA LIVNPHDSIG MCEALQRALA MPLDERKRRH DIDMQALRKN DLGVWRDSFL RDLRSVPLAG SPSDASGTST DSAKRTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcc gactgatcgt cgtgtcgaat cgcgtggcaa cgccgactga aacgagaggc tcggcgggcg ggctggccgt cggcgtgttc ggcgcgctga aggatgcggg cggggtgtgg ttcgggtgga gcggcgacgt cgtcagcgag acggtggcga acgccgggcc cacgctcgaa acggacggca gcgtcacgtt tgccaccaca ggcctcacgc gcaaggacta cgaccagtac taccggggct tttcgaacgc gacgctgtgg cccgtcttcc actatcgcaa cgatctcgct cgctacgaac gcgaggaata cgcaggctac cggcgggtca atgcgtggct ctcgcacaag ctcatcaagc tgctggagcc tgacgacatt atctgggtcc acgactatca cctcatcccc ttcgcggaag cgctgcgatc cgagggcgtc aggaatcgca tcggtttttt cctgcacatt ccgtttccgg cgccgcagat cctgttgaac attccgccgc atgaagaact ggtgaagtcg ttatgttgct acgacctgct cggttttcag accgaaaacg acgagctcgc gtttcatgac tatctgcgcc ggcacgcacg cggcaccgtc tcggctgacg gacacgcgca agcgttcggt cggcagatgc gcacgggcgt ctaccggatc ggcgtgttcc ccgacgagat tgccgagcag gcaaagcgct atgagagccg tcagcacgtg ctcgacctca agcagagcct ggaagggcgc aagctgatca tgagcgtcga ccgtctcgac tattcgaagg ggctggtcga gcgcttccgc gcattcgagg cgctgctgga gcgctcgccc gaatggcgcg gcaatgtcac acttgtgcag attgcgccgc ccacgcgctc cgacgtcacg acgtaccagg acatccgtca gcaactcgaa tacgaagccg ggcgtatcaa cggaaaatac tcaggcctgg actacacgcc cattcgttat ttgaatcagc aatacgaccg ctggaagctg atgtcgctgt tccgcgagtc gcaggtcgga ttcgtcacgc ccttgcacga cgggatgaat ctcgtcgcga aggaatatgt cgccgcccag aacccggacg atccaggtgt gctcgtactg tcgatgttcg cgggcgcggc cgccgaactg gaaggggcat tgatcgtgaa tcctcacgat tcgatcggca tgtgcgaagc gttgcagcgt gcgctcgcga tgccgctcga cgaacgcaag cggcgtcacg atatcgacat gcaggcgctg cgcaagaacg atctcggtgt gtggcgcgac tcgttcctgc gcgatctgcg cagcgtgccg cttgccgggt cgccgagcga cgcttccgga acctcgacag actcagcaaa aaggacaaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuphA.00046.c.B11.GE40110
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG RLIVVSNRVA TPTETRGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLETDG SVTFATTGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYAGYRRV NAWLSHKLIK LLEPDDIIWV HDYHLIPFAE ALRSEGVRNR IGFFLHIPFP APQILLNIPP HEELVKSLCC YDLLGFQTEN DELAFHDYLR RHARGTVSAD GHAQAFGRQM RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE ALLERSPEWR GNVTLVQIAP PTRSDVTTYQ DIRQQLEYEA GRINGKYSGL DYTPIRYLNQ QYDRWKLMSL FRESQVGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSMF AGAAAELEGA LIVNPHDSIG MCEALQRALA MPLDERKRRH DIDMQALRKN DLGVWRDSFL RDLRSVPLAG SPSDASGTST
Validated NT Sequence
ctgtcgaggt tccggaagcg tcgctcggcg acccggcaag cggcacgctg cgcagatcgc gcaggaacga gtcgcgccac acaccgagat cgttcttgcg cagcgcctgc atgtcgatat cgtgacgccg cttgcgttcg tcgagcggca tcgcgagcgc acgctgcaac gcttcgcaca tgccgatcga atcgtgagga ttcacgatca atgccccttc cagttcggcg gccgcgcccg cgaacatcga cagtacgagc acacctggat cgtccgggtt ctgggcggcg acatattcct tcgcgacgag attcatcccg tcgtgcaagg gcgtgacgaa tccgacctgc gactcgcgga acagcgacat cagcttccag cggtcgtatt gctgattcaa ataacgaatg ggcgtgtagt ccaggcctga gtattttccg ttgatacgcc cggcttcgta ttcgagttgc tgacggatgt cctggtacgt cgtgacgtcg gagcgcgtgg gcggcgcaat ctgcacaagt gtgacattgc cgcgccattc gggcgagcgc tccagcagcg cctcgaatgc gcggaagcgc tcgaccagcc ccttcgaata gtcgagacgg tcgacgctca tgatcagctt gcgcccttcc aggctctgct tgaggtcgag cacgtgctga cggctctcat agcgctttgc ctgctcggca atctcgtcgg ggaacacgcc gatccggtag acgcccgtgc gcatctgccg accgaacgct tgcgcgtgtc cgtcagccga gacggtgccg cgtgcgtgcc ggcgcagata gtcatgaaac gcgagctcgt cgttttcggt ctgaaaaccg agcaggtcgt agcaacataa cgacttcacc agttcttcat gcggcggaat gttcaacagg atctgcggcg ccggaaacgg aatgtgcagg aaaaaaccga tgcgattcct gacgccctcg gatcgcagcg cttccgcgaa ggggatgagg tgatagtcgt ggacccagat aatgtcgtca ggctccagca gcttgatgag cttgtgcgag agccacgcat tgacccgccg gtagcctgcg tattcctcgc gttcgtagcg agcgagatcg ttgcgatagt ggaagacggg ccacagcgtc gcgttcgaaa agccccggta gtactggtcg tagtccttgc gcgtgaggcc tgtggtggca aacgtgacgc tgccgtccgt ttcgagcgtg ggcccggcgt tcgccaccgt ctcgctgacg acgtcgccgc tccacccgaa ccacaccccg cccgcatcct tcagcgcgcc gaacacgccg acggccagcc cgcccgccga gcctctcgtt tcagtcggcg ttgccacgcg attcgacacg acgatcagtc ggcccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMG RLIVVSNRVA TPTETRGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLETDG SVTFATTGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYAGYRRV NAWLSHKLIK LLEPDDIIWV HDYHLIPFAE ALRSEGVRNR IGFFLHIPFP APQILLNIPP HEELVKSLCC YDLLGFQTEN DELAFHDYLR RHARGTVSAD GHAQAFGRQM RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE ALLERSPEWR GNVTLVQIAP PTRSDVTTYQ DIRQQLEYEA GRINGKYSGL DYTPIRYLNQ QYDRWKLMSL FRESQVGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSMF AGAAAELEGA LIVNPHDSIG MCEALQRALA MPLDERKRRH DIDMQALRKN DLGVWRDSFL RDLRSVPLAG SPSDASGTST DSAKRTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcc gactgatcgt cgtgtcgaat cgcgtggcaa cgccgactga aacgagaggc tcggcgggcg ggctggccgt cggcgtgttc ggcgcgctga aggatgcggg cggggtgtgg ttcgggtgga gcggcgacgt cgtcagcgag acggtggcga acgccgggcc cacgctcgaa acggacggca gcgtcacgtt tgccaccaca ggcctcacgc gcaaggacta cgaccagtac taccggggct tttcgaacgc gacgctgtgg cccgtcttcc actatcgcaa cgatctcgct cgctacgaac gcgaggaata cgcaggctac cggcgggtca atgcgtggct ctcgcacaag ctcatcaagc tgctggagcc tgacgacatt atctgggtcc acgactatca cctcatcccc ttcgcggaag cgctgcgatc cgagggcgtc aggaatcgca tcggtttttt cctgcacatt ccgtttccgg cgccgcagat cctgttgaac attccgccgc atgaagaact ggtgaagtcg ttatgttgct acgacctgct cggttttcag accgaaaacg acgagctcgc gtttcatgac tatctgcgcc ggcacgcacg cggcaccgtc tcggctgacg gacacgcgca agcgttcggt cggcagatgc gcacgggcgt ctaccggatc ggcgtgttcc ccgacgagat tgccgagcag gcaaagcgct atgagagccg tcagcacgtg ctcgacctca agcagagcct ggaagggcgc aagctgatca tgagcgtcga ccgtctcgac tattcgaagg ggctggtcga gcgcttccgc gcattcgagg cgctgctgga gcgctcgccc gaatggcgcg gcaatgtcac acttgtgcag attgcgccgc ccacgcgctc cgacgtcacg acgtaccagg acatccgtca gcaactcgaa tacgaagccg ggcgtatcaa cggaaaatac tcaggcctgg actacacgcc cattcgttat ttgaatcagc aatacgaccg ctggaagctg atgtcgctgt tccgcgagtc gcaggtcgga ttcgtcacgc ccttgcacga cgggatgaat ctcgtcgcga aggaatatgt cgccgcccag aacccggacg atccaggtgt gctcgtactg tcgatgttcg cgggcgcggc cgccgaactg gaaggggcat tgatcgtgaa tcctcacgat tcgatcggca tgtgcgaagc gttgcagcgt gcgctcgcga tgccgctcga cgaacgcaag cggcgtcacg atatcgacat gcaggcgctg cgcaagaacg atctcggtgt gtggcgcgac tcgttcctgc gcgatctgcg cagcgtgccg cttgccgggt cgccgagcga cgcttccgga acctcgacag actcagcaaa aaggacaaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuphA.00046.c.B11.PS37993
PURIFICATION DATe: 7/26/2016
CONCENTRATION: 42mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG RLIVVSNRVA TPTETRGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLETDG SVTFATTGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYAGYRRV NAWLSHKLIK LLEPDDIIWV HDYHLIPFAE ALRSEGVRNR IGFFLHIPFP APQILLNIPP HEELVKSLCC YDLLGFQTEN DELAFHDYLR RHARGTVSAD GHAQAFGRQM RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE ALLERSPEWR GNVTLVQIAP PTRSDVTTYQ DIRQQLEYEA GRINGKYSGL DYTPIRYLNQ QYDRWKLMSL FRESQVGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSMF AGAAAELEGA LIVNPHDSIG MCEALQRALA MPLDERKRRH DIDMQALRKN DLGVWRDSFL RDLRSVPLAG SPSDASGTST
Validated NT Sequence
ctgtcgaggt tccggaagcg tcgctcggcg acccggcaag cggcacgctg cgcagatcgc gcaggaacga gtcgcgccac acaccgagat cgttcttgcg cagcgcctgc atgtcgatat cgtgacgccg cttgcgttcg tcgagcggca tcgcgagcgc acgctgcaac gcttcgcaca tgccgatcga atcgtgagga ttcacgatca atgccccttc cagttcggcg gccgcgcccg cgaacatcga cagtacgagc acacctggat cgtccgggtt ctgggcggcg acatattcct tcgcgacgag attcatcccg tcgtgcaagg gcgtgacgaa tccgacctgc gactcgcgga acagcgacat cagcttccag cggtcgtatt gctgattcaa ataacgaatg ggcgtgtagt ccaggcctga gtattttccg ttgatacgcc cggcttcgta ttcgagttgc tgacggatgt cctggtacgt cgtgacgtcg gagcgcgtgg gcggcgcaat ctgcacaagt gtgacattgc cgcgccattc gggcgagcgc tccagcagcg cctcgaatgc gcggaagcgc tcgaccagcc ccttcgaata gtcgagacgg tcgacgctca tgatcagctt gcgcccttcc aggctctgct tgaggtcgag cacgtgctga cggctctcat agcgctttgc ctgctcggca atctcgtcgg ggaacacgcc gatccggtag acgcccgtgc gcatctgccg accgaacgct tgcgcgtgtc cgtcagccga gacggtgccg cgtgcgtgcc ggcgcagata gtcatgaaac gcgagctcgt cgttttcggt ctgaaaaccg agcaggtcgt agcaacataa cgacttcacc agttcttcat gcggcggaat gttcaacagg atctgcggcg ccggaaacgg aatgtgcagg aaaaaaccga tgcgattcct gacgccctcg gatcgcagcg cttccgcgaa ggggatgagg tgatagtcgt ggacccagat aatgtcgtca ggctccagca gcttgatgag cttgtgcgag agccacgcat tgacccgccg gtagcctgcg tattcctcgc gttcgtagcg agcgagatcg ttgcgatagt ggaagacggg ccacagcgtc gcgttcgaaa agccccggta gtactggtcg tagtccttgc gcgtgaggcc tgtggtggca aacgtgacgc tgccgtccgt ttcgagcgtg ggcccggcgt tcgccaccgt ctcgctgacg acgtcgccgc tccacccgaa ccacaccccg cccgcatcct tcagcgcgcc gaacacgccg acggccagcc cgcccgccga gcctctcgtt tcagtcggcg ttgccacgcg attcgacacg acgatcagtc ggcccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMG RLIVVSNRVA TPTETRGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLETDG SVTFATTGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYAGYRRV NAWLSHKLIK LLEPDDIIWV HDYHLIPFAE ALRSEGVRNR IGFFLHIPFP APQILLNIPP HEELVKSLCC YDLLGFQTEN DELAFHDYLR RHARGTVSAD GHAQAFGRQM RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE ALLERSPEWR GNVTLVQIAP PTRSDVTTYQ DIRQQLEYEA GRINGKYSGL DYTPIRYLNQ QYDRWKLMSL FRESQVGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSMF AGAAAELEGA LIVNPHDSIG MCEALQRALA MPLDERKRRH DIDMQALRKN DLGVWRDSFL RDLRSVPLAG SPSDASGTST DSAKRTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGCC GACTGATCGT CGTGTCGAAT CGCGTGGCAA CGCCGACTGA AACGAGAGGC TCGGCGGGCG GGCTGGCCGT CGGCGTGTTC GGCGCGCTGA AGGATGCGGG CGGGGTGTGG TTCGGGTGGA GCGGCGACGT CGTCAGCGAG ACGGTGGCGA ACGCCGGGCC CACGCTCGAA ACGGACGGCA GCGTCACGTT TGCCACCACA GGCCTCACGC GCAAGGACTA CGACCAGTAC TACCGGGGCT TTTCGAACGC GACGCTGTGG CCCGTCTTCC ACTATCGCAA CGATCTCGCT CGCTACGAAC GCGAGGAATA CGCAGGCTAC CGGCGGGTCA ATGCGTGGCT CTCGCACAAG CTCATCAAGC TGCTGGAGCC TGACGACATT ATCTGGGTCC ACGACTATCA CCTCATCCCC TTCGCGGAAG CGCTGCGATC CGAGGGCGTC AGGAATCGCA TCGGTTTTTT CCTGCACATT CCGTTTCCGG CGCCGCAGAT CCTGTTGAAC ATTCCGCCGC ATGAAGAACT GGTGAAGTCG TTATGTTGCT ACGACCTGCT CGGTTTTCAG ACCGAAAACG ACGAGCTCGC GTTTCATGAC TATCTGCGCC GGCACGCACG CGGCACCGTC TCGGCTGACG GACACGCGCA AGCGTTCGGT CGGCAGATGC GCACGGGCGT CTACCGGATC GGCGTGTTCC CCGACGAGAT TGCCGAGCAG GCAAAGCGCT ATGAGAGCCG TCAGCACGTG CTCGACCTCA AGCAGAGCCT GGAAGGGCGC AAGCTGATCA TGAGCGTCGA CCGTCTCGAC TATTCGAAGG GGCTGGTCGA GCGCTTCCGC GCATTCGAGG CGCTGCTGGA GCGCTCGCCC GAATGGCGCG GCAATGTCAC ACTTGTGCAG ATTGCGCCGC CCACGCGCTC CGACGTCACG ACGTACCAGG ACATCCGTCA GCAACTCGAA TACGAAGCCG GGCGTATCAA CGGAAAATAC TCAGGCCTGG ACTACACGCC CATTCGTTAT TTGAATCAGC AATACGACCG CTGGAAGCTG ATGTCGCTGT TCCGCGAGTC GCAGGTCGGA TTCGTCACGC CCTTGCACGA CGGGATGAAT CTCGTCGCGA AGGAATATGT CGCCGCCCAG AACCCGGACG ATCCAGGTGT GCTCGTACTG TCGATGTTCG CGGGCGCGGC CGCCGAACTG GAAGGGGCAT TGATCGTGAA TCCTCACGAT TCGATCGGCA TGTGCGAAGC GTTGCAGCGT GCGCTCGCGA TGCCGCTCGA CGAACGCAAG CGGCGTCACG ATATCGACAT GCAGGCGCTG CGCAAGAACG ATCTCGGTGT GTGGCGCGAC TCGTTCCTGC GCGATCTGCG CAGCGTGCCG CTTGCCGGGT CGCCGAGCGA CGCTTCCGGA ACCTCGACAG ACTCAGCAAA AAGGACAAAG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for BuphA.00046.c.B11.PS37994
PURIFICATION DATe: 7/26/2016
CONCENTRATION: 39mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG RLIVVSNRVA TPTETRGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLETDG SVTFATTGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYAGYRRV NAWLSHKLIK LLEPDDIIWV HDYHLIPFAE ALRSEGVRNR IGFFLHIPFP APQILLNIPP HEELVKSLCC YDLLGFQTEN DELAFHDYLR RHARGTVSAD GHAQAFGRQM RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE ALLERSPEWR GNVTLVQIAP PTRSDVTTYQ DIRQQLEYEA GRINGKYSGL DYTPIRYLNQ QYDRWKLMSL FRESQVGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSMF AGAAAELEGA LIVNPHDSIG MCEALQRALA MPLDERKRRH DIDMQALRKN DLGVWRDSFL RDLRSVPLAG SPSDASGTST
Validated NT Sequence
ctgtcgaggt tccggaagcg tcgctcggcg acccggcaag cggcacgctg cgcagatcgc gcaggaacga gtcgcgccac acaccgagat cgttcttgcg cagcgcctgc atgtcgatat cgtgacgccg cttgcgttcg tcgagcggca tcgcgagcgc acgctgcaac gcttcgcaca tgccgatcga atcgtgagga ttcacgatca atgccccttc cagttcggcg gccgcgcccg cgaacatcga cagtacgagc acacctggat cgtccgggtt ctgggcggcg acatattcct tcgcgacgag attcatcccg tcgtgcaagg gcgtgacgaa tccgacctgc gactcgcgga acagcgacat cagcttccag cggtcgtatt gctgattcaa ataacgaatg ggcgtgtagt ccaggcctga gtattttccg ttgatacgcc cggcttcgta ttcgagttgc tgacggatgt cctggtacgt cgtgacgtcg gagcgcgtgg gcggcgcaat ctgcacaagt gtgacattgc cgcgccattc gggcgagcgc tccagcagcg cctcgaatgc gcggaagcgc tcgaccagcc ccttcgaata gtcgagacgg tcgacgctca tgatcagctt gcgcccttcc aggctctgct tgaggtcgag cacgtgctga cggctctcat agcgctttgc ctgctcggca atctcgtcgg ggaacacgcc gatccggtag acgcccgtgc gcatctgccg accgaacgct tgcgcgtgtc cgtcagccga gacggtgccg cgtgcgtgcc ggcgcagata gtcatgaaac gcgagctcgt cgttttcggt ctgaaaaccg agcaggtcgt agcaacataa cgacttcacc agttcttcat gcggcggaat gttcaacagg atctgcggcg ccggaaacgg aatgtgcagg aaaaaaccga tgcgattcct gacgccctcg gatcgcagcg cttccgcgaa ggggatgagg tgatagtcgt ggacccagat aatgtcgtca ggctccagca gcttgatgag cttgtgcgag agccacgcat tgacccgccg gtagcctgcg tattcctcgc gttcgtagcg agcgagatcg ttgcgatagt ggaagacggg ccacagcgtc gcgttcgaaa agccccggta gtactggtcg tagtccttgc gcgtgaggcc tgtggtggca aacgtgacgc tgccgtccgt ttcgagcgtg ggcccggcgt tcgccaccgt ctcgctgacg acgtcgccgc tccacccgaa ccacaccccg cccgcatcct tcagcgcgcc gaacacgccg acggccagcc cgcccgccga gcctctcgtt tcagtcggcg ttgccacgcg attcgacacg acgatcagtc ggcccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMG RLIVVSNRVA TPTETRGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLETDG SVTFATTGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYAGYRRV NAWLSHKLIK LLEPDDIIWV HDYHLIPFAE ALRSEGVRNR IGFFLHIPFP APQILLNIPP HEELVKSLCC YDLLGFQTEN DELAFHDYLR RHARGTVSAD GHAQAFGRQM RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE ALLERSPEWR GNVTLVQIAP PTRSDVTTYQ DIRQQLEYEA GRINGKYSGL DYTPIRYLNQ QYDRWKLMSL FRESQVGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSMF AGAAAELEGA LIVNPHDSIG MCEALQRALA MPLDERKRRH DIDMQALRKN DLGVWRDSFL RDLRSVPLAG SPSDASGTST DSAKRTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGCC GACTGATCGT CGTGTCGAAT CGCGTGGCAA CGCCGACTGA AACGAGAGGC TCGGCGGGCG GGCTGGCCGT CGGCGTGTTC GGCGCGCTGA AGGATGCGGG CGGGGTGTGG TTCGGGTGGA GCGGCGACGT CGTCAGCGAG ACGGTGGCGA ACGCCGGGCC CACGCTCGAA ACGGACGGCA GCGTCACGTT TGCCACCACA GGCCTCACGC GCAAGGACTA CGACCAGTAC TACCGGGGCT TTTCGAACGC GACGCTGTGG CCCGTCTTCC ACTATCGCAA CGATCTCGCT CGCTACGAAC GCGAGGAATA CGCAGGCTAC CGGCGGGTCA ATGCGTGGCT CTCGCACAAG CTCATCAAGC TGCTGGAGCC TGACGACATT ATCTGGGTCC ACGACTATCA CCTCATCCCC TTCGCGGAAG CGCTGCGATC CGAGGGCGTC AGGAATCGCA TCGGTTTTTT CCTGCACATT CCGTTTCCGG CGCCGCAGAT CCTGTTGAAC ATTCCGCCGC ATGAAGAACT GGTGAAGTCG TTATGTTGCT ACGACCTGCT CGGTTTTCAG ACCGAAAACG ACGAGCTCGC GTTTCATGAC TATCTGCGCC GGCACGCACG CGGCACCGTC TCGGCTGACG GACACGCGCA AGCGTTCGGT CGGCAGATGC GCACGGGCGT CTACCGGATC GGCGTGTTCC CCGACGAGAT TGCCGAGCAG GCAAAGCGCT ATGAGAGCCG TCAGCACGTG CTCGACCTCA AGCAGAGCCT GGAAGGGCGC AAGCTGATCA TGAGCGTCGA CCGTCTCGAC TATTCGAAGG GGCTGGTCGA GCGCTTCCGC GCATTCGAGG CGCTGCTGGA GCGCTCGCCC GAATGGCGCG GCAATGTCAC ACTTGTGCAG ATTGCGCCGC CCACGCGCTC CGACGTCACG ACGTACCAGG ACATCCGTCA GCAACTCGAA TACGAAGCCG GGCGTATCAA CGGAAAATAC TCAGGCCTGG ACTACACGCC CATTCGTTAT TTGAATCAGC AATACGACCG CTGGAAGCTG ATGTCGCTGT TCCGCGAGTC GCAGGTCGGA TTCGTCACGC CCTTGCACGA CGGGATGAAT CTCGTCGCGA AGGAATATGT CGCCGCCCAG AACCCGGACG ATCCAGGTGT GCTCGTACTG TCGATGTTCG CGGGCGCGGC CGCCGAACTG GAAGGGGCAT TGATCGTGAA TCCTCACGAT TCGATCGGCA TGTGCGAAGC GTTGCAGCGT GCGCTCGCGA TGCCGCTCGA CGAACGCAAG CGGCGTCACG ATATCGACAT GCAGGCGCTG CGCAAGAACG ATCTCGGTGT GTGGCGCGAC TCGTTCCTGC GCGATCTGCG CAGCGTGCCG CTTGCCGGGT CGCCGAGCGA CGCTTCCGGA ACCTCGACAG ACTCAGCAAA AAGGACAAAG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for BuphA.00046.c.B1.PS38009
PURIFICATION DATe: 8/10/2016
CONCENTRATION: 22.25mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG RLIVVSNRVA TPTETRGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLETDG SVTFATTGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYAGYRRV NAWLSHKLIK LLEPDDIIWV HDYHLIPFAE ALRSEGVRNR IGFFLHIPFP APQILLNIPP HEELVKSLCC YDLLGFQTEN DELAFHDYLR RHARGTVSAD GHAQAFGRQM RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE ALLERSPEWR GNVTLVQIAP PTRSDVTTYQ DIRQQLEYEA GRINGKYSGL DYTPIRYLNQ QYDRWKLMSL FRESQVGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSMF AGAAAELEGA LIVNPHDSIG MCEALQRALA MPLDERKRRH DIDMQALRKN DLGVWRDSFL RDLRSVPLAG SPSDASGTST DSAKRTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGCC GACTGATCGT CGTGTCGAAT CGCGTGGCAA CGCCGACTGA AACGAGAGGC TCGGCGGGCG GGCTGGCCGT CGGCGTGTTC GGCGCGCTGA AGGATGCGGG CGGGGTGTGG TTCGGGTGGA GCGGCGACGT CGTCAGCGAG ACGGTGGCGA ACGCCGGGCC CACGCTCGAA ACGGACGGCA GCGTCACGTT TGCCACCACA GGCCTCACGC GCAAGGACTA CGACCAGTAC TACCGGGGCT TTTCGAACGC GACGCTGTGG CCCGTCTTCC ACTATCGCAA CGATCTCGCT CGCTACGAAC GCGAGGAATA CGCAGGCTAC CGGCGGGTCA ATGCGTGGCT CTCGCACAAG CTCATCAAGC TGCTGGAGCC TGACGACATT ATCTGGGTCC ACGACTATCA CCTCATCCCC TTCGCGGAAG CGCTGCGATC CGAGGGCGTC AGGAATCGCA TCGGTTTTTT CCTGCACATT CCGTTTCCGG CGCCGCAGAT CCTGTTGAAC ATTCCGCCGC ATGAAGAACT GGTGAAGTCG TTATGTTGCT ACGACCTGCT CGGTTTTCAG ACCGAAAACG ACGAGCTCGC GTTTCATGAC TATCTGCGCC GGCACGCACG CGGCACCGTC TCGGCTGACG GACACGCGCA AGCGTTCGGT CGGCAGATGC GCACGGGCGT CTACCGGATC GGCGTGTTCC CCGACGAGAT TGCCGAGCAG GCAAAGCGCT ATGAGAGCCG TCAGCACGTG CTCGACCTCA AGCAGAGCCT GGAAGGGCGC AAGCTGATCA TGAGCGTCGA CCGTCTCGAC TATTCGAAGG GGCTGGTCGA GCGCTTCCGC GCATTCGAGG CGCTGCTGGA GCGCTCGCCC GAATGGCGCG GCAATGTCAC ACTTGTGCAG ATTGCGCCGC CCACGCGCTC CGACGTCACG ACGTACCAGG ACATCCGTCA GCAACTCGAA TACGAAGCCG GGCGTATCAA CGGAAAATAC TCAGGCCTGG ACTACACGCC CATTCGTTAT TTGAATCAGC AATACGACCG CTGGAAGCTG ATGTCGCTGT TCCGCGAGTC GCAGGTCGGA TTCGTCACGC CCTTGCACGA CGGGATGAAT CTCGTCGCGA AGGAATATGT CGCCGCCCAG AACCCGGACG ATCCAGGTGT GCTCGTACTG TCGATGTTCG CGGGCGCGGC CGCCGAACTG GAAGGGGCAT TGATCGTGAA TCCTCACGAT TCGATCGGCA TGTGCGAAGC GTTGCAGCGT GCGCTCGCGA TGCCGCTCGA CGAACGCAAG CGGCGTCACG ATATCGACAT GCAGGCGCTG CGCAAGAACG ATCTCGGTGT GTGGCGCGAC TCGTTCCTGC GCGATCTGCG CAGCGTGCCG CTTGCCGGGT CGCCGAGCGA CGCTTCCGGA ACCTCGACAG ACTCAGCAAA AAGGACAAAG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT