BuphA.00046.a

Alpha,alpha-trehalose-phosphate synthase (UDP-forming)

CENTER ID: BuphA.00046.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00046.a.B1.GE40106 Full length( BuphA.00046.a ) 1 473
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.2282
RefSeq: YP_001858366.1
UniProt: B2JEB2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLIVVSNR VAPTQEGRPA AGGLAIGVLD ALKETGGVWF GWSGEIAAEP STPVIEKQGN VTYATVGLTK RDYDQYYRGF SNATLWPTFH YRNDLSRYDR QEYAGYLRVN VTLAKQLKEL IRPDDIIWVH DYHLLPFARC LRDLGVKNPI GFFLHIPFPV PEVMRTVPPH EELVKCMCSY DVVGFQTDAD RQSFVDYIER GNHGTSSDDN MIHAYGRFLK VAAYPIGIYP DAIAKAAEQF SDRKQVRNMR DAMRGRKLIM SVDRLDYSKG LVERFQAFER LLQNAPGWHG RVSLVQIAPP TRADVQTYQR IRQNLEGEAG RINGRFAQLD WTPIQYLNRK YERNLLMALF RQSQVGYVTP LRDGMNLVAK EYVASQDPAD PGVLVLSQFA GAAEQLPGAL VVNPFDLSQM AEALERALSM PLAERQARHA DMMAPLRENN LSVWRDTFLA DLRHVATASS VTEKAVKRAD VTA
NT Sequence
ATGAGCAGAT TGATCGTCGT TTCAAACCGC GTCGCGCCGA CTCAGGAAGG GCGCCCCGCA GCGGGCGGTC TCGCAATCGG AGTGCTGGAT GCACTGAAGG AAACGGGAGG TGTGTGGTTC GGATGGAGTG GCGAGATCGC CGCCGAGCCT TCGACGCCCG TCATCGAGAA GCAGGGCAAT GTGACCTACG CCACCGTGGG ACTGACCAAG CGCGATTACG ACCAGTACTA CCGCGGTTTC TCGAATGCGA CGCTGTGGCC GACTTTCCAT TATCGCAACG ACCTGTCGCG CTACGACCGG CAGGAATACG CGGGGTATCT GCGGGTGAAC GTGACGCTCG CGAAGCAGCT CAAGGAACTG ATCAGGCCCG ACGACATCAT CTGGGTGCAC GACTATCACC TGCTGCCGTT CGCGCGCTGC CTGCGCGATC TCGGCGTGAA AAATCCGATC GGCTTTTTCC TGCATATTCC GTTTCCCGTG CCGGAAGTGA TGCGCACCGT GCCGCCGCAC GAAGAACTCG TGAAATGCAT GTGCAGCTAT GACGTGGTCG GTTTCCAGAC GGATGCGGAC CGGCAATCGT TCGTCGATTA CATCGAGCGC GGCAATCACG GCACATCGAG CGACGACAAC ATGATTCACG CGTACGGCCG CTTCCTGAAG GTGGCCGCCT ATCCGATCGG CATCTATCCC GATGCGATCG CGAAAGCCGC CGAGCAGTTC AGCGACCGGA AGCAGGTGCG CAACATGCGC GACGCGATGC GGGGCCGCAA GCTGATCATG AGCGTCGACC GGCTCGACTA TTCGAAGGGG CTGGTCGAGC GTTTCCAGGC GTTCGAGCGC CTGCTGCAGA ATGCGCCCGG CTGGCATGGC CGCGTGTCGC TCGTGCAGAT CGCGCCGCCG ACGCGCGCCG ACGTGCAGAC CTACCAGCGC ATTCGTCAGA ACCTCGAAGG CGAAGCGGGG CGGATCAACG GCCGGTTTGC GCAGCTCGAC TGGACGCCGA TTCAGTACCT GAACCGCAAA TACGAGCGCA ATCTGCTGAT GGCGCTGTTC CGCCAGTCGC AGGTCGGCTA TGTGACGCCG CTGCGCGACG GCATGAATCT CGTCGCGAAG GAATATGTCG CGTCGCAGGA TCCCGCGGAT CCCGGCGTGC TCGTGCTGTC GCAGTTCGCG GGCGCGGCCG AGCAGCTGCC CGGCGCGCTG GTCGTGAATC CGTTCGATCT GTCGCAGATG GCCGAGGCGC TGGAGCGCGC GCTGTCGATG CCGCTCGCCG AGCGCCAGGC GCGGCACGCG GACATGATGG CGCCGCTGCG CGAGAACAAT CTGTCGGTGT GGCGCGATAC GTTCCTCGCC GACCTGCGCC ATGTCGCGAC GGCTTCGTCG GTGACGGAGA AGGCCGTCAA GCGCGCTGAC GTGACGGCA
Details for BuphA.00046.a.B1.GE40106
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 54kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS RLIVVSNRVA PTQEGRPAAG GLAIGVLDAL KETGGVWFGW SGEIAAEPST PVIEKQGNVT YATVGLTKRD YDQYYRGFSN ATLWPTFHYR NDLSRYDRQE YAGYLRVNVT LAKQLKELIR PDDIIWVHDY HLLPFARCLR DLGVKNPIGF FLHIPFPVPE VMRTVPPHEE LVKCMCSYDV VGFQTDADRQ SFVDYIERGN HGTSSDDNMI HAYGRFLKVA AYPIGIYPDA IAKAAEQFSD RKQVRNMRDA MRGRKLIMSV DRLDYSKGLV ERFQAFERLL QNAPGWHGRV SLVQIAPPTR ADVQTYQRIR QNLEGEAGRI NGRFAQLDWT PIQYLNRKYE RNLLMALFRQ SQVGYVTPLR DGMNLVAKEY VASQDPADPG VLVLSQFAGA AEQLPGALVV NPFDLSQMAE ALERALSMPL AERQARHADM MAPLRENNLS VWRDTFLADL RHVATASSVT EKAVKRADVT A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca gattgatcgt cgtttcaaac cgcgtcgcgc cgactcagga agggcgcccc gcagcgggcg gtctcgcaat cggagtgctg gatgcactga aggaaacggg aggtgtgtgg ttcggatgga gtggcgagat cgccgccgag ccttcgacgc ccgtcatcga gaagcagggc aatgtgacct acgccaccgt gggactgacc aagcgcgatt acgaccagta ctaccgcggt ttctcgaatg cgacgctgtg gccgactttc cattatcgca acgacctgtc gcgctacgac cggcaggaat acgcggggta tctgcgggtg aacgtgacgc tcgcgaagca gctcaaggaa ctgatcaggc ccgacgacat catctgggtg cacgactatc acctgctgcc gttcgcgcgc tgcctgcgcg atctcggcgt gaaaaatccg atcggctttt tcctgcatat tccgtttccc gtgccggaag tgatgcgcac cgtgccgccg cacgaagaac tcgtgaaatg catgtgcagc tatgacgtgg tcggtttcca gacggatgcg gaccggcaat cgttcgtcga ttacatcgag cgcggcaatc acggcacatc gagcgacgac aacatgattc acgcgtacgg ccgcttcctg aaggtggccg cctatccgat cggcatctat cccgatgcga tcgcgaaagc cgccgagcag ttcagcgacc ggaagcaggt gcgcaacatg cgcgacgcga tgcggggccg caagctgatc atgagcgtcg accggctcga ctattcgaag gggctggtcg agcgtttcca ggcgttcgag cgcctgctgc agaatgcgcc cggctggcat ggccgcgtgt cgctcgtgca gatcgcgccg ccgacgcgcg ccgacgtgca gacctaccag cgcattcgtc agaacctcga aggcgaagcg gggcggatca acggccggtt tgcgcagctc gactggacgc cgattcagta cctgaaccgc aaatacgagc gcaatctgct gatggcgctg ttccgccagt cgcaggtcgg ctatgtgacg ccgctgcgcg acggcatgaa tctcgtcgcg aaggaatatg tcgcgtcgca ggatcccgcg gatcccggcg tgctcgtgct gtcgcagttc gcgggcgcgg ccgagcagct gcccggcgcg ctggtcgtga atccgttcga tctgtcgcag atggccgagg cgctggagcg cgcgctgtcg atgccgctcg ccgagcgcca ggcgcggcac gcggacatga tggcgccgct gcgcgagaac aatctgtcgg tgtggcgcga tacgttcctc gccgacctgc gccatgtcgc gacggcttcg tcggtgacgg agaaggccgt caagcgcgct gacgtgacgg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat