SSGCID: Target Page

BuphA.00039.b

Methionine aminopeptidase

CENTER ID:	BuphA.00039.b
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00039.b.B1.GE40105	Full length( BuphA.00039.b )		1	270

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00039.b.B1.PS38015	Full length( BuphA.00039.b )		1	270

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.1423
RefSeq:	YP_001857567.1
UniProt:	B2JIC7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAITLKNEDD IAQMRIAGRL ASEVLDYITP FVKPGVTTGE LDRLCHEYMT NVQGTVPAPL NYQPPGYPPF PKAVCTSVND VICHGIPGDK ALKNGDALNI DVTVIKNGYY GDTSRMFIVG EGSIMAKRLV QTTYECMWLG IEQVRPGAHL GDIGHAIQKH AESLGYSVVR EYCGHGIGKV FHDEPQILHY GRPGTGIELQ AGMIFTIEPM INAGRRDIRT MPDQWTVKTK DRSLSAQWEH TILVTPTGYE VLTVSEGTPA RPQIVAAATA

NT Sequence

ATGGCTATTA CGCTCAAAAA CGAAGACGAT ATCGCGCAGA TGCGCATCGC CGGACGGCTC GCGAGCGAGG TGCTCGACTA CATCACGCCG TTCGTCAAGC CCGGCGTCAC GACGGGCGAA CTCGACCGTC TTTGCCACGA ATACATGACG AACGTGCAAG GCACGGTTCC CGCGCCGCTG AATTACCAGC CGCCCGGCTA TCCGCCTTTC CCGAAGGCCG TCTGCACGTC CGTCAACGAC GTGATCTGCC ACGGCATCCC CGGCGACAAG GCGCTGAAGA ACGGCGACGC GCTGAATATC GACGTCACCG TGATCAAGAA CGGCTATTAC GGCGACACGA GCCGCATGTT CATCGTCGGC GAGGGCTCGA TCATGGCGAA GCGCCTCGTC CAGACCACGT ATGAATGCAT GTGGCTCGGC ATCGAGCAGG TGCGCCCCGG CGCGCATCTC GGCGACATCG GCCATGCGAT CCAGAAACAT GCGGAAAGCC TCGGCTACAG CGTCGTGCGC GAATATTGCG GGCACGGCAT CGGCAAGGTC TTCCACGACG AGCCGCAAAT CCTGCACTAC GGCCGCCCTG GCACGGGCAT CGAACTGCAG GCGGGCATGA TTTTCACGAT CGAGCCGATG ATCAATGCCG GCCGCCGCGA TATCCGCACG ATGCCCGACC AGTGGACCGT CAAGACCAAG GACCGCAGCC TGTCCGCCCA ATGGGAGCAC ACGATCCTCG TCACGCCGAC GGGCTACGAA GTGCTGACCG TGTCGGAAGG CACGCCCGCG CGTCCGCAAA TCGTCGCCGC CGCCACGGCC

Details for BuphA.00039.b.B1.GE40105

HARVESTED ON:	4/6/2016
SEQUENCED ON:	4/13/2016
EXPECTED MW:	31kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA ITLKNEDDIA QMRIAGRLAS EVLDYITPFV KPGVTTGELD RLCHEYMTNV QGTVPAPLNY QPPGYPPFPK AVCTSVNDVI CHGIPGDKAL KNGDALNIDV TVIKNGYYGD TSRMFIVGEG SIMAKRLVQT TYECMWLGIE QVRPGAHLGD IGHAIQKHAE SLGYSVVREY CGHGIGKVFH DEPQILHYGR PGTGIELQAG MIFTIEPMIN AGRRDIRTMP DQWTVKTKDR SLSAQWEHTI LVTPTGYEVL TVSEGTPARP QIVAAATA

Validated NT Sequence

atcttactca cttaggccgt ggcggcggcg acgatttgcg gacgcgcggg cgtgccttcc gacacggtca gcacttcgta gcccgtcggc gtgacgagga tcgtgtgctc ccattgggcg gacaggctgc ggtccttggt cttgacggtc cactggtcgg gcatcgtgcg gatatcgcgg cggccggcat tgatcatcgg ctcgatcgtg aaaatcatgc ccgcctgcag ttcgatgccc gtgccagggc ggccgtagtg caggatttgc ggctcgtcgt ggaagacctt gccgatgccg tgcccgcaat attcgcgcac gacgctgtag ccgaggcttt ccgcatgttt ctggatcgca tggccgatgt cgccgagatg cgcgccgggg cgcacctgct cgatgccgag ccacatgcat tcatacgtgg tctggacgag gcgcttcgcc atgatcgagc cctcgccgac gatgaacatg cggctcgtgt cgccgtaata gccgttcttg atcacggtga cgtcgatatt cagcgcgtcg ccgttcttca gcgccttgtc gccggggatg ccgtggcaga tcacgtcgtt gacggacgtg cagacggcct tcgggaaagg cggatagccg ggcggctggt aattcagcgg cgcgggaacc gtgccttgca cgttcgtcat gtattcgtgg caaagacggt cgagttcgcc cgtcgtgacg ccgggcttga cgaacggcgt gatgtagtcg agcacctcgc tcgcgagccg tccggcgatg cgcatctgcg cgatatcgtc ttcgtttttg agcgtaatag ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta ttacgctcaa aaacgaagac gatatcgcgc agatgcgcat cgccggacgg ctcgcgagcg aggtgctcga ctacatcacg ccgttcgtca agcccggcgt cacgacgggc gaactcgacc gtctttgcca cgaatacatg acgaacgtgc aaggcacggt tcccgcgccg ctgaattacc agccgcccgg ctatccgcct ttcccgaagg ccgtctgcac gtccgtcaac gacgtgatct gccacggcat ccccggcgac aaggcgctga agaacggcga cgcgctgaat atcgacgtca ccgtgatcaa gaacggctat tacggcgaca cgagccgcat gttcatcgtc ggcgagggct cgatcatggc gaagcgcctc gtccagacca cgtatgaatg catgtggctc ggcatcgagc aggtgcgccc cggcgcgcat ctcggcgaca tcggccatgc gatccagaaa catgcggaaa gcctcggcta cagcgtcgtg cgcgaatatt gcgggcacgg catcggcaag gtcttccacg acgagccgca aatcctgcac tacggccgcc ctggcacggg catcgaactg caggcgggca tgattttcac gatcgagccg atgatcaatg ccggccgccg cgatatccgc acgatgcccg accagtggac cgtcaagacc aaggaccgca gcctgtccgc ccaatgggag cacacgatcc tcgtcacgcc gacgggctac gaagtgctga ccgtgtcgga aggcacgccc gcgcgtccgc aaatcgtcgc cgccgccacg gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BuphA.00039.b.B1.PS38015

PURIFICATION DATe:	8/2/2016
CONCENTRATION:	52.24mg/ml
OBSERVED MW:	31kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTA TTACGCTCAA AAACGAAGAC GATATCGCGC AGATGCGCAT CGCCGGACGG CTCGCGAGCG AGGTGCTCGA CTACATCACG CCGTTCGTCA AGCCCGGCGT CACGACGGGC GAACTCGACC GTCTTTGCCA CGAATACATG ACGAACGTGC AAGGCACGGT TCCCGCGCCG CTGAATTACC AGCCGCCCGG CTATCCGCCT TTCCCGAAGG CCGTCTGCAC GTCCGTCAAC GACGTGATCT GCCACGGCAT CCCCGGCGAC AAGGCGCTGA AGAACGGCGA CGCGCTGAAT ATCGACGTCA CCGTGATCAA GAACGGCTAT TACGGCGACA CGAGCCGCAT GTTCATCGTC GGCGAGGGCT CGATCATGGC GAAGCGCCTC GTCCAGACCA CGTATGAATG CATGTGGCTC GGCATCGAGC AGGTGCGCCC CGGCGCGCAT CTCGGCGACA TCGGCCATGC GATCCAGAAA CATGCGGAAA GCCTCGGCTA CAGCGTCGTG CGCGAATATT GCGGGCACGG CATCGGCAAG GTCTTCCACG ACGAGCCGCA AATCCTGCAC TACGGCCGCC CTGGCACGGG CATCGAACTG CAGGCGGGCA TGATTTTCAC GATCGAGCCG ATGATCAATG CCGGCCGCCG CGATATCCGC ACGATGCCCG ACCAGTGGAC CGTCAAGACC AAGGACCGCA GCCTGTCCGC CCAATGGGAG CACACGATCC TCGTCACGCC GACGGGCTAC GAAGTGCTGA CCGTGTCGGA AGGCACGCCC GCGCGTCCGC AAATCGTCGC CGCCGCCACG GCCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT