BuphA.00027.f
Acyl-CoA dehydrogenase domain protein
| CENTER ID: | BuphA.00027.f |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00027.f.B1.GE39389 | Full length( BuphA.00027.f ) | 1 | 401 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00027.f.B1.PS02558 | Full length( BuphA.00027.f ) | 1 | 401 |
Structures
5IDU
DEPOSITED: 2/24/2016
DETERMINATION: XRay
CLONE: BuphA.00027.f.B1.GE39389
PROTEIN: BuphA.00027.f.B1.PS02558
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | NO_GROUP |
| PATRIC ID: | fig|391038.7.peg.4295 |
| RefSeq: | YP_001860241.1 |
| UniProt: | B2JPK4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAANLIDPHG ALAWPFFEAR HRELAAGIEA WATQHLACVQ HDDTDTTCRK LVRALGEAGW LKYGVGGAQY GGHGDTIDTR AVCLLRETLA NHDGLADFAL AMQGLGSGAI TLAGTHEQKI RYLPRVSKGE AIAAFALSEP DAGSDVAAMS LQARAEGDCY VIDGDKTWIS NGGIADFYVV FARTGEAPGA RGISAFIVDA DTPGLQIAER IDVIAPHPLA RLHFDSARVP RSQMLGAPGE GFKIAMRTLD VFRTSVAAAS LGFARRALQE GLARAASRKM FGQTLGDFQL TQTKLAQMAL TIDSSALLVY RAAWLRDQGE NVTREAAMAK WHASEGAQQV IDAAVQLWGG MGVQSGTTVE RLYREIRALR IYEGATEVQQ LIVGRDLLKA HAAQRQQERA S
NT Sequence
TTGGCTGCGA ACCTCATCGA TCCGCATGGC GCGTTGGCCT GGCCATTCTT CGAAGCGCGT CATCGTGAAC TGGCCGCAGG TATAGAGGCA TGGGCCACGC AGCATCTCGC GTGTGTGCAG CATGACGACA CGGACACGAC GTGCCGCAAG CTCGTGCGCG CGCTTGGCGA AGCAGGCTGG CTGAAATATG GCGTAGGCGG CGCGCAATAC GGAGGACATG GCGACACGAT CGACACGCGC GCCGTGTGTC TGCTGCGCGA AACGCTCGCG AACCACGACG GCCTCGCCGA CTTCGCGCTG GCGATGCAAG GGCTCGGTTC CGGCGCGATC ACGCTGGCGG GCACGCATGA ACAGAAGATT CGCTACCTGC CGCGCGTCTC AAAGGGCGAA GCGATTGCCG CATTCGCGCT GTCGGAGCCC GACGCAGGTT CCGATGTCGC GGCCATGTCG TTGCAGGCGC GCGCCGAAGG CGACTGCTAT GTGATCGACG GCGACAAGAC GTGGATCTCG AACGGCGGTA TCGCGGACTT CTACGTTGTG TTCGCAAGAA CGGGCGAAGC ACCCGGCGCG CGCGGCATCA GCGCGTTCAT CGTCGACGCC GATACGCCGG GCCTGCAGAT CGCGGAGCGC ATCGACGTGA TCGCGCCGCA TCCGCTCGCG CGTCTGCATT TCGACAGTGC GCGCGTGCCG CGCAGCCAGA TGCTCGGCGC GCCCGGCGAA GGCTTCAAGA TCGCGATGCG TACGCTCGAC GTTTTTCGCA CGTCGGTTGC GGCGGCGTCG CTGGGTTTTG CGCGACGCGC ATTGCAGGAA GGGCTCGCAC GCGCCGCGTC GCGCAAGATG TTCGGCCAGA CGCTCGGCGA TTTTCAACTG ACGCAGACGA AGCTGGCACA GATGGCGTTG ACGATCGACA GCAGCGCGCT GCTCGTCTAT CGCGCCGCAT GGCTGCGCGA TCAGGGCGAA AACGTCACGC GCGAAGCCGC GATGGCGAAA TGGCACGCAA GCGAAGGCGC GCAACAGGTG ATCGACGCCG CCGTGCAACT GTGGGGCGGC ATGGGCGTGC AGAGCGGCAC GACGGTCGAG CGGCTGTATC GCGAGATTCG CGCGCTGCGC ATCTATGAAG GCGCGACGGA AGTGCAGCAG TTGATCGTCG GACGCGATCT GCTGAAGGCT CACGCTGCGC AACGTCAACA GGAGCGCGCG TCA
Details for BuphA.00027.f.B1.GE39389
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHML AANLIDPHGA LAWPFFEARH RELAAGIEAW ATQHLACVQH DDTDTTCRKL VRALGEAGWL KYGVGGAQYG GHGDTIDTRA VCLLRETLAN HDGLADFALA MQGLGSGAIT LAGTHEQKIR YLPRVSKGEA IAAFALSEPD AGSDVAAMSL QARAEGDCYV IDGDKTWISN GGIADFYVVF ARTGEAPGAR GISAFIVDAD TPGLQIAERI DVIAPHPLAR LHFDSARVPR SQMLGAPGEG FKIAMRTLDV FRTSVAAASL GFARRALQEG LARAASRKMF GQTLGDFQLT QTKLAQMALT IDSSALLVYR AAWLRDQGEN VTREAAMAKW HASEGAQQVI DAAVQLWGGM GVQSGTTVER LYREIRALRI YEGATEVQQL IVGRDLLKAH AA
Validated NT Sequence
tgcgcagcgt gagccttcag cagatcgcgt ccgacgatca actgctgcac ttccgtcgcg ccttcataga tgcgcagcgc gcgaatctcg cgatacagcc gctcgaccgt cgtgccgctc tgcacgccca tgccgcccca cagttgcacg gcggcgtcga tcacctgttg cgcgccttcg cttgcgtgcc atttcgccat cgcggcttcg cgcgtgacgt tttcgccctg atcgcgcagc catgcggcgc gatagacgag cagcgcgctg ctgtcgatcg tcaacgccat ctgtgccagc ttcgtctgcg tcagttgaaa atcgccgagc gtctggccga acatcttgcg cgacgcggcg cgtgcgagcc cttcctgcaa tgcgcgtcgc gcaaaaccca gcgacgccgc cgcaaccgac gtgcgaaaaa cgtcgagcgt acgcatcgcg atcttgaagc cttcgccggg cgcgccgagc atctggctgc gcggcacgcg cgcactgtcg aaatgcagac gcgcgagcgg atgcggcgcg atcacgtcga tgcgctccgc gatctgcagg cccggcgtat cggcgtcgac gatgaacgcg ctgatgccgc gcgcgccggg tgcttcgccc gttcttgcga acacaacgta gaagtccgcg ataccgccgt tcgagatcca cgtcttgtcg ccgtcgatca catagcagtc gccttcggcg cgcgcctgca acgacatggc cgcgacatcg gaacctgcgt cgggctccga cagcgcgaat gcggcaatcg cttcgccctt tgagacgcgc ggcaggtagc gaatcttctg ttcatgcgtg cccgccagcg tgatcgcgcc ggaaccgagc ccttgcatcg ccagcgcgaa gtcggcgagg ccgtcgtggt tcgcgagcgt ttcgcgcagc agacacacgg cgcgcgtgtc gatcgtgtcg ccatgtcctc cgtattgcgc gccgcctacg ccatatttca gccagcctgc ttcgccaagc gcgcgcacga gcttgcggca cgtcgtgtcc gtgtcgtcat gctgcacaca cgcgagatgc tgcgtggccc atgcctctat acctgcggcc agttcacgat gacgcgcttc gaagaatggc caggccaacg cgccatgcgg atcgatgagg ttcgcagcca acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMA ANLIDPHGAL AWPFFEARHR ELAAGIEAWA TQHLACVQHD DTDTTCRKLV RALGEAGWLK YGVGGAQYGG HGDTIDTRAV CLLRETLANH DGLADFALAM QGLGSGAITL AGTHEQKIRY LPRVSKGEAI AAFALSEPDA GSDVAAMSLQ ARAEGDCYVI DGDKTWISNG GIADFYVVFA RTGEAPGARG ISAFIVDADT PGLQIAERID VIAPHPLARL HFDSARVPRS QMLGAPGEGF KIAMRTLDVF RTSVAAASLG FARRALQEGL ARAASRKMFG QTLGDFQLTQ TKLAQMALTI DSSALLVYRA AWLRDQGENV TREAAMAKWH ASEGAQQVID AAVQLWGGMG VQSGTTVERL YREIRALRIY EGATEVQQLI VGRDLLKAHA AQRQQERAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggctg cgaacctcat cgatccgcat ggcgcgttgg cctggccatt cttcgaagcg cgtcatcgtg aactggccgc aggtatagag gcatgggcca cgcagcatct cgcgtgtgtg cagcatgacg acacggacac gacgtgccgc aagctcgtgc gcgcgcttgg cgaagcaggc tggctgaaat atggcgtagg cggcgcgcaa tacggaggac atggcgacac gatcgacacg cgcgccgtgt gtctgctgcg cgaaacgctc gcgaaccacg acggcctcgc cgacttcgcg ctggcgatgc aagggctcgg ttccggcgcg atcacgctgg cgggcacgca tgaacagaag attcgctacc tgccgcgcgt ctcaaagggc gaagcgattg ccgcattcgc gctgtcggag cccgacgcag gttccgatgt cgcggccatg tcgttgcagg cgcgcgccga aggcgactgc tatgtgatcg acggcgacaa gacgtggatc tcgaacggcg gtatcgcgga cttctacgtt gtgttcgcaa gaacgggcga agcacccggc gcgcgcggca tcagcgcgtt catcgtcgac gccgatacgc cgggcctgca gatcgcggag cgcatcgacg tgatcgcgcc gcatccgctc gcgcgtctgc atttcgacag tgcgcgcgtg ccgcgcagcc agatgctcgg cgcgcccggc gaaggcttca agatcgcgat gcgtacgctc gacgtttttc gcacgtcggt tgcggcggcg tcgctgggtt ttgcgcgacg cgcattgcag gaagggctcg cacgcgccgc gtcgcgcaag atgttcggcc agacgctcgg cgattttcaa ctgacgcaga cgaagctggc acagatggcg ttgacgatcg acagcagcgc gctgctcgtc tatcgcgccg catggctgcg cgatcagggc gaaaacgtca cgcgcgaagc cgcgatggcg aaatggcacg caagcgaagg cgcgcaacag gtgatcgacg ccgccgtgca actgtggggc ggcatgggcg tgcagagcgg cacgacggtc gagcggctgt atcgcgagat tcgcgcgctg cgcatctatg aaggcgcgac ggaagtgcag cagttgatcg tcggacgcga tctgctgaag gctcacgctg cgcaacgtca acaggagcgc gcgtcatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BuphA.00027.f.B1.PS02558
| PURIFICATION DATe: | 12/22/2015 |
| CONCENTRATION: | 42.2mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML AANLIDPHGA LAWPFFEARH RELAAGIEAW ATQHLACVQH DDTDTTCRKL VRALGEAGWL KYGVGGAQYG GHGDTIDTRA VCLLRETLAN HDGLADFALA MQGLGSGAIT LAGTHEQKIR YLPRVSKGEA IAAFALSEPD AGSDVAAMSL QARAEGDCYV IDGDKTWISN GGIADFYVVF ARTGEAPGAR GISAFIVDAD TPGLQIAERI DVIAPHPLAR LHFDSARVPR SQMLGAPGEG FKIAMRTLDV FRTSVAAASL GFARRALQEG LARAASRKMF GQTLGDFQLT QTKLAQMALT IDSSALLVYR AAWLRDQGEN VTREAAMAKW HASEGAQQVI DAAVQLWGGM GVQSGTTVER LYREIRALRI YEGATEVQQL IVGRDLLKAH AA
Validated NT Sequence
tgcgcagcgt gagccttcag cagatcgcgt ccgacgatca actgctgcac ttccgtcgcg ccttcataga tgcgcagcgc gcgaatctcg cgatacagcc gctcgaccgt cgtgccgctc tgcacgccca tgccgcccca cagttgcacg gcggcgtcga tcacctgttg cgcgccttcg cttgcgtgcc atttcgccat cgcggcttcg cgcgtgacgt tttcgccctg atcgcgcagc catgcggcgc gatagacgag cagcgcgctg ctgtcgatcg tcaacgccat ctgtgccagc ttcgtctgcg tcagttgaaa atcgccgagc gtctggccga acatcttgcg cgacgcggcg cgtgcgagcc cttcctgcaa tgcgcgtcgc gcaaaaccca gcgacgccgc cgcaaccgac gtgcgaaaaa cgtcgagcgt acgcatcgcg atcttgaagc cttcgccggg cgcgccgagc atctggctgc gcggcacgcg cgcactgtcg aaatgcagac gcgcgagcgg atgcggcgcg atcacgtcga tgcgctccgc gatctgcagg cccggcgtat cggcgtcgac gatgaacgcg ctgatgccgc gcgcgccggg tgcttcgccc gttcttgcga acacaacgta gaagtccgcg ataccgccgt tcgagatcca cgtcttgtcg ccgtcgatca catagcagtc gccttcggcg cgcgcctgca acgacatggc cgcgacatcg gaacctgcgt cgggctccga cagcgcgaat gcggcaatcg cttcgccctt tgagacgcgc ggcaggtagc gaatcttctg ttcatgcgtg cccgccagcg tgatcgcgcc ggaaccgagc ccttgcatcg ccagcgcgaa gtcggcgagg ccgtcgtggt tcgcgagcgt ttcgcgcagc agacacacgg cgcgcgtgtc gatcgtgtcg ccatgtcctc cgtattgcgc gccgcctacg ccatatttca gccagcctgc ttcgccaagc gcgcgcacga gcttgcggca cgtcgtgtcc gtgtcgtcat gctgcacaca cgcgagatgc tgcgtggccc atgcctctat acctgcggcc agttcacgat gacgcgcttc gaagaatggc caggccaacg cgccatgcgg atcgatgagg ttcgcagcca acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMA ANLIDPHGAL AWPFFEARHR ELAAGIEAWA TQHLACVQHD DTDTTCRKLV RALGEAGWLK YGVGGAQYGG HGDTIDTRAV CLLRETLANH DGLADFALAM QGLGSGAITL AGTHEQKIRY LPRVSKGEAI AAFALSEPDA GSDVAAMSLQ ARAEGDCYVI DGDKTWISNG GIADFYVVFA RTGEAPGARG ISAFIVDADT PGLQIAERID VIAPHPLARL HFDSARVPRS QMLGAPGEGF KIAMRTLDVF RTSVAAASLG FARRALQEGL ARAASRKMFG QTLGDFQLTQ TKLAQMALTI DSSALLVYRA AWLRDQGENV TREAAMAKWH ASEGAQQVID AAVQLWGGMG VQSGTTVERL YREIRALRIY EGATEVQQLI VGRDLLKAHA AQRQQERAS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGCTG CGAACCTCAT CGATCCGCAT GGCGCGTTGG CCTGGCCATT CTTCGAAGCG CGTCATCGTG AACTGGCCGC AGGTATAGAG GCATGGGCCA CGCAGCATCT CGCGTGTGTG CAGCATGACG ACACGGACAC GACGTGCCGC AAGCTCGTGC GCGCGCTTGG CGAAGCAGGC TGGCTGAAAT ATGGCGTAGG CGGCGCGCAA TACGGAGGAC ATGGCGACAC GATCGACACG CGCGCCGTGT GTCTGCTGCG CGAAACGCTC GCGAACCACG ACGGCCTCGC CGACTTCGCG CTGGCGATGC AAGGGCTCGG TTCCGGCGCG ATCACGCTGG CGGGCACGCA TGAACAGAAG ATTCGCTACC TGCCGCGCGT CTCAAAGGGC GAAGCGATTG CCGCATTCGC GCTGTCGGAG CCCGACGCAG GTTCCGATGT CGCGGCCATG TCGTTGCAGG CGCGCGCCGA AGGCGACTGC TATGTGATCG ACGGCGACAA GACGTGGATC TCGAACGGCG GTATCGCGGA CTTCTACGTT GTGTTCGCAA GAACGGGCGA AGCACCCGGC GCGCGCGGCA TCAGCGCGTT CATCGTCGAC GCCGATACGC CGGGCCTGCA GATCGCGGAG CGCATCGACG TGATCGCGCC GCATCCGCTC GCGCGTCTGC ATTTCGACAG TGCGCGCGTG CCGCGCAGCC AGATGCTCGG CGCGCCCGGC GAAGGCTTCA AGATCGCGAT GCGTACGCTC GACGTTTTTC GCACGTCGGT TGCGGCGGCG TCGCTGGGTT TTGCGCGACG CGCATTGCAG GAAGGGCTCG CACGCGCCGC GTCGCGCAAG ATGTTCGGCC AGACGCTCGG CGATTTTCAA CTGACGCAGA CGAAGCTGGC ACAGATGGCG TTGACGATCG ACAGCAGCGC GCTGCTCGTC TATCGCGCCG CATGGCTGCG CGATCAGGGC GAAAACGTCA CGCGCGAAGC CGCGATGGCG AAATGGCACG CAAGCGAAGG CGCGCAACAG GTGATCGACG CCGCCGTGCA ACTGTGGGGC GGCATGGGCG TGCAGAGCGG CACGACGGTC GAGCGGCTGT ATCGCGAGAT TCGCGCGCTG CGCATCTATG AAGGCGCGAC GGAAGTGCAG CAGTTGATCG TCGGACGCGA TCTGCTGAAG GCTCACGCTG CGCAACGTCA ACAGGAGCGC GCGTCATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T