BuphA.00024.b

Taurine dioxygenase

CENTER ID: BuphA.00024.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00024.b.B1.GE40102 Full length( BuphA.00024.b ) 1 316
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00024.b.B1.PS37981 Full length( BuphA.00024.b ) 1 316

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.3311
RefSeq: YP_001859330.1
UniProt: B2JR58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNTHAALAS SKGGALAGHT APETLDVRRV AGRIGAEIRG IELSGSIGAP TFDAIHAALL KHKVLFFRGQ QHLDDAAQEA FARRFGETVA HPTVPSVDGS RHLLELDSQH GARANSWHTD VTFVDAYPKI SILRAVVIPP FGGDTVWANT AAAYEHLPEP LRRLADTLWA LHTNAYDYAS SHVDADGEQL KRYREVFTST VYETEHPVVR VHPETGERSL VLGHFVQRLK GLSASESAHL LQVFHDHVTR LENTVRWSWT QGDVAIWDNR ATQHYAINDY GDAHRVVRRA TVHGDVPVSI DGRHSVVVRG AGATLQ
NT Sequence
ATGTCGAATA CGCACGCGGC CCTCGCCTCA TCGAAGGGCG GCGCCCTCGC CGGGCATACG GCACCCGAAA CGCTCGATGT GCGACGCGTC GCCGGCCGCA TCGGCGCAGA GATTCGAGGC ATCGAGCTGT CCGGGTCCAT CGGTGCGCCG ACCTTCGATG CGATCCACGC CGCGCTACTG AAGCACAAGG TTCTATTCTT TCGTGGCCAG CAGCATCTGG ACGACGCCGC ACAGGAAGCG TTCGCGCGAC GCTTCGGCGA AACTGTCGCA CACCCGACCG TGCCGTCCGT CGATGGCAGC CGCCATCTGC TCGAACTCGA TTCGCAGCAC GGCGCGCGGG CGAACTCGTG GCACACGGAC GTCACCTTCG TCGACGCCTA TCCGAAGATT TCGATCCTTC GCGCGGTCGT GATTCCGCCC TTTGGCGGCG ACACCGTCTG GGCCAATACG GCCGCCGCGT ACGAGCATCT GCCCGAGCCG CTGCGCCGTC TGGCCGATAC ACTGTGGGCG CTGCACACGA ACGCGTACGA CTACGCGTCG TCACACGTGG ATGCCGACGG CGAGCAGCTG AAGCGCTACC GCGAAGTGTT CACGTCGACG GTGTACGAGA CGGAGCATCC CGTCGTGCGC GTGCATCCGG AAACGGGCGA ACGCTCGCTC GTGCTCGGTC ATTTCGTGCA GCGCCTGAAG GGACTGTCGG CGAGCGAGTC GGCGCATCTG CTGCAGGTCT TCCACGACCA CGTCACGCGG CTCGAAAACA CCGTGCGCTG GAGCTGGACG CAAGGTGACG TCGCTATCTG GGACAACCGC GCGACCCAGC ACTACGCGAT CAACGACTAC GGCGACGCGC ACCGCGTCGT CCGTCGCGCG ACGGTGCACG GCGACGTGCC CGTGAGTATC GACGGGCGGC ACAGCGTGGT GGTTCGGGGT GCAGGCGCGA CGCTGCAG
Details for BuphA.00024.b.B1.GE40102
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS NTHAALASSK GGALAGHTAP ETLDVRRVAG RIGAEIRGIE LSGSIGAPTF DAIHAALLKH KVLFFRGQQH LDDAAQEAFA RRFGETVAHP TVPSVDGSRH LLELDSQHGA RANSWHTDVT FVDAYPKISI LRAVVIPPFG GDTVWANTAA AYEHLPEPLR RLADTLWALH TNAYDYASSH VDADGEQLKR YREVFTSTVY ETEHPVVRVH PETGERSLVL GHFVQRLKGL SASESAHLLQ VFHDHVTRLE NTVRWSWTQG DVAIWDNRAT QHYAINDYGD AHRVVRRATV HGDVPVSIDG RHSVVVXXXG ATLQ
Validated NT Sequence
cttactgcag cgtcgcgcct gnnnnnnnaa ccaccacgct gtgccgcccg tcgatactca cgggcacgtc gccgtgcacc gtcgcgcgac ggacgacgcg gtgcgcgtcg ccgtagtcgt tgatcgcgta gtgctgggtc gcgcggttgt cccagatagc gacgtcacct tgcgtccagc tccagcgcac ggtgttttcg agccgcgtga cgtggtcgtg gaagacctgc agcagatgcg ccgactcgct cgccgacagt cccttcaggc gctgcacgaa atgaccgagc acgagcgagc gttcgcccgt ttccggatgc acgcgcacga cgggatgctc cgtctcgtac accgtcgacg tgaacacttc gcggtagcgc ttcagctgct cgccgtcggc atccacgtgt gacgacgcgt agtcgtacgc gttcgtgtgc agcgcccaca gtgtatcggc cagacggcgc agcggctcgg gcagatgctc gtacgcggcg gccgtattgg cccagacggt gtcgccgcca aagggcggaa tcacgaccgc gcgaaggatc gaaatcttcg gataggcgtc gacgaaggtg acgtccgtgt gccacgagtt cgcccgcgcg ccgtgctgcg aatcgagttc gagcagatgg cggctgccat cgacggacgg cacggtcggg tgtgcgacag tttcgccgaa gcgtcgcgcg aacgcttcct gtgcggcgtc gtccagatgc tgctggccac gaaagaatag aaccttgtgc ttcagtagcg cggcgtggat cgcatcgaag gtcggcgcac cgatggaccc ggacagctcg atgcctcgaa tctctgcgcc gatgcggccg gcgacgcgtc gcacatcgag cgtttcgggt gccgtatgcc cggcgagggc gccgcccttc gatgaggcga gggccgcgtg cgtattcgac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMS NTHAALASSK GGALAGHTAP ETLDVRRVAG RIGAEIRGIE LSGSIGAPTF DAIHAALLKH KVLFFRGQQH LDDAAQEAFA RRFGETVAHP TVPSVDGSRH LLELDSQHGA RANSWHTDVT FVDAYPKISI LRAVVIPPFG GDTVWANTAA AYEHLPEPLR RLADTLWALH TNAYDYASSH VDADGEQLKR YREVFTSTVY ETEHPVVRVH PETGERSLVL GHFVQRLKGL SASESAHLLQ VFHDHVTRLE NTVRWSWTQG DVAIWDNRAT QHYAINDYGD AHRVVRRATV HGDVPVSIDG RHSVVVRGAG ATLQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga atacgcacgc ggccctcgcc tcatcgaagg gcggcgccct cgccgggcat acggcacccg aaacgctcga tgtgcgacgc gtcgccggcc gcatcggcgc agagattcga ggcatcgagc tgtccgggtc catcggtgcg ccgaccttcg atgcgatcca cgccgcgcta ctgaagcaca aggttctatt ctttcgtggc cagcagcatc tggacgacgc cgcacaggaa gcgttcgcgc gacgcttcgg cgaaactgtc gcacacccga ccgtgccgtc cgtcgatggc agccgccatc tgctcgaact cgattcgcag cacggcgcgc gggcgaactc gtggcacacg gacgtcacct tcgtcgacgc ctatccgaag atttcgatcc ttcgcgcggt cgtgattccg ccctttggcg gcgacaccgt ctgggccaat acggccgccg cgtacgagca tctgcccgag ccgctgcgcc gtctggccga tacactgtgg gcgctgcaca cgaacgcgta cgactacgcg tcgtcacacg tggatgccga cggcgagcag ctgaagcgct accgcgaagt gttcacgtcg acggtgtacg agacggagca tcccgtcgtg cgcgtgcatc cggaaacggg cgaacgctcg ctcgtgctcg gtcatttcgt gcagcgcctg aagggactgt cggcgagcga gtcggcgcat ctgctgcagg tcttccacga ccacgtcacg cggctcgaaa acaccgtgcg ctggagctgg acgcaaggtg acgtcgctat ctgggacaac cgcgcgaccc agcactacgc gatcaacgac tacggcgacg cgcaccgcgt cgtccgtcgc gcgacggtgc acggcgacgt gcccgtgagt atcgacgggc ggcacagcgt ggtggttcgg ggtgcaggcg cgacgctgca gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuphA.00024.b.B1.PS37981
PURIFICATION DATe: 7/20/2016
CONCENTRATION: 41.6mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS NTHAALASSK GGALAGHTAP ETLDVRRVAG RIGAEIRGIE LSGSIGAPTF DAIHAALLKH KVLFFRGQQH LDDAAQEAFA RRFGETVAHP TVPSVDGSRH LLELDSQHGA RANSWHTDVT FVDAYPKISI LRAVVIPPFG GDTVWANTAA AYEHLPEPLR RLADTLWALH TNAYDYASSH VDADGEQLKR YREVFTSTVY ETEHPVVRVH PETGERSLVL GHFVQRLKGL SASESAHLLQ VFHDHVTRLE NTVRWSWTQG DVAIWDNRAT QHYAINDYGD AHRVVRRATV HGDVPVSIDG RHSVVVXXXG ATLQ
Validated NT Sequence
cttactgcag cgtcgcgcct gnnnnnnnaa ccaccacgct gtgccgcccg tcgatactca cgggcacgtc gccgtgcacc gtcgcgcgac ggacgacgcg gtgcgcgtcg ccgtagtcgt tgatcgcgta gtgctgggtc gcgcggttgt cccagatagc gacgtcacct tgcgtccagc tccagcgcac ggtgttttcg agccgcgtga cgtggtcgtg gaagacctgc agcagatgcg ccgactcgct cgccgacagt cccttcaggc gctgcacgaa atgaccgagc acgagcgagc gttcgcccgt ttccggatgc acgcgcacga cgggatgctc cgtctcgtac accgtcgacg tgaacacttc gcggtagcgc ttcagctgct cgccgtcggc atccacgtgt gacgacgcgt agtcgtacgc gttcgtgtgc agcgcccaca gtgtatcggc cagacggcgc agcggctcgg gcagatgctc gtacgcggcg gccgtattgg cccagacggt gtcgccgcca aagggcggaa tcacgaccgc gcgaaggatc gaaatcttcg gataggcgtc gacgaaggtg acgtccgtgt gccacgagtt cgcccgcgcg ccgtgctgcg aatcgagttc gagcagatgg cggctgccat cgacggacgg cacggtcggg tgtgcgacag tttcgccgaa gcgtcgcgcg aacgcttcct gtgcggcgtc gtccagatgc tgctggccac gaaagaatag aaccttgtgc ttcagtagcg cggcgtggat cgcatcgaag gtcggcgcac cgatggaccc ggacagctcg atgcctcgaa tctctgcgcc gatgcggccg gcgacgcgtc gcacatcgag cgtttcgggt gccgtatgcc cggcgagggc gccgcccttc gatgaggcga gggccgcgtg cgtattcgac atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMS NTHAALASSK GGALAGHTAP ETLDVRRVAG RIGAEIRGIE LSGSIGAPTF DAIHAALLKH KVLFFRGQQH LDDAAQEAFA RRFGETVAHP TVPSVDGSRH LLELDSQHGA RANSWHTDVT FVDAYPKISI LRAVVIPPFG GDTVWANTAA AYEHLPEPLR RLADTLWALH TNAYDYASSH VDADGEQLKR YREVFTSTVY ETEHPVVRVH PETGERSLVL GHFVQRLKGL SASESAHLLQ VFHDHVTRLE NTVRWSWTQG DVAIWDNRAT QHYAINDYGD AHRVVRRATV HGDVPVSIDG RHSVVVRGAG ATLQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCGA ATACGCACGC GGCCCTCGCC TCATCGAAGG GCGGCGCCCT CGCCGGGCAT ACGGCACCCG AAACGCTCGA TGTGCGACGC GTCGCCGGCC GCATCGGCGC AGAGATTCGA GGCATCGAGC TGTCCGGGTC CATCGGTGCG CCGACCTTCG ATGCGATCCA CGCCGCGCTA CTGAAGCACA AGGTTCTATT CTTTCGTGGC CAGCAGCATC TGGACGACGC CGCACAGGAA GCGTTCGCGC GACGCTTCGG CGAAACTGTC GCACACCCGA CCGTGCCGTC CGTCGATGGC AGCCGCCATC TGCTCGAACT CGATTCGCAG CACGGCGCGC GGGCGAACTC GTGGCACACG GACGTCACCT TCGTCGACGC CTATCCGAAG ATTTCGATCC TTCGCGCGGT CGTGATTCCG CCCTTTGGCG GCGACACCGT CTGGGCCAAT ACGGCCGCCG CGTACGAGCA TCTGCCCGAG CCGCTGCGCC GTCTGGCCGA TACACTGTGG GCGCTGCACA CGAACGCGTA CGACTACGCG TCGTCACACG TGGATGCCGA CGGCGAGCAG CTGAAGCGCT ACCGCGAAGT GTTCACGTCG ACGGTGTACG AGACGGAGCA TCCCGTCGTG CGCGTGCATC CGGAAACGGG CGAACGCTCG CTCGTGCTCG GTCATTTCGT GCAGCGCCTG AAGGGACTGT CGGCGAGCGA GTCGGCGCAT CTGCTGCAGG TCTTCCACGA CCACGTCACG CGGCTCGAAA ACACCGTGCG CTGGAGCTGG ACGCAAGGTG ACGTCGCTAT CTGGGACAAC CGCGCGACCC AGCACTACGC GATCAACGAC TACGGCGACG CGCACCGCGT CGTCCGTCGC GCGACGGTGC ACGGCGACGT GCCCGTGAGT ATCGACGGGC GGCACAGCGT GGTGGTTCGG GGTGCAGGCG CGACGCTGCA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT