BuphA.00024.a

Taurine dioxygenase

CENTER ID: BuphA.00024.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00024.a.B1.GE40101 Full length( BuphA.00024.a ) 1 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.3804
RefSeq: YP_001859789.1
UniProt: B2JM53

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTTVSPHRH AAPRVQSSGA LQTFDIVAFD APVGAEVLGL DLNEPLSADD FARIHRAHLD YHVLVFREQR ITPEQHVAFS RRFGPLQIHV LRQFQLPGHP EVLIVSNIRE NGQPIGLGDA GHFWHSDLSY KEKPSLGSLL HAQELPSEGG DTLFANMHLA WDTLPEHLRK AVEGRTAEHT YLAKYAELQK RSPWRPNLSA EQVAEVKPVV HPVVRTHPET GRKALFVSEH FTTHIVGLPE DESRDLLGAL FVHSVRDEHI YRHQWAEHDL VFWDNRSLMH LAAGTPDHLR RKLYRTTIEG DAPF
NT Sequence
GTGACCACCA CCGTTTCACC CCATCGCCAC GCGGCGCCGC GCGTGCAATC GTCCGGCGCG CTTCAGACAT TCGACATCGT TGCGTTTGAT GCACCCGTCG GTGCCGAAGT GCTCGGCCTC GATCTGAATG AGCCGCTTTC CGCCGACGAT TTCGCGCGTA TTCATCGCGC GCATCTCGAC TATCACGTGC TCGTGTTTCG CGAGCAGCGG ATTACGCCCG AACAGCATGT CGCGTTCAGC CGCCGCTTCG GGCCGCTGCA AATCCACGTG CTGCGTCAGT TCCAGTTGCC CGGCCATCCC GAAGTGCTGA TCGTATCGAA CATTCGCGAG AACGGTCAGC CGATCGGACT CGGCGATGCC GGTCACTTCT GGCATTCGGA TCTGTCGTAC AAAGAGAAAC CGAGTCTCGG CTCGCTGCTT CACGCGCAAG AGTTGCCGTC GGAAGGCGGT GACACGCTGT TCGCGAACAT GCACCTGGCG TGGGACACAT TGCCCGAGCA TCTGCGCAAG GCCGTCGAAG GACGCACGGC GGAGCATACG TACCTCGCGA AATATGCGGA GCTGCAAAAG CGCAGCCCGT GGCGGCCGAA TCTCTCGGCT GAGCAGGTCG CCGAAGTGAA ACCCGTCGTG CATCCCGTCG TGCGCACGCA TCCGGAAACG GGCCGCAAGG CGCTTTTCGT CAGCGAGCAT TTCACTACGC ATATTGTCGG TCTTCCCGAG GACGAAAGCC GCGATCTGCT TGGCGCGCTG TTTGTGCACA GCGTGCGCGA CGAGCACATC TATCGTCATC AGTGGGCTGA GCACGATCTC GTGTTCTGGG ACAACCGCTC GTTGATGCAC CTCGCCGCGG GCACGCCCGA TCATCTGCGC CGCAAGCTGT ACCGCACGAC GATCGAAGGC GACGCGCCGT TC
Details for BuphA.00024.a.B1.GE40101
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TTTVSPHRHA AXRVQSSGAL QTFDIVAFDA PVGAEVLGLD LNEPLSADDF ARIHRAHLDY HVLVFREQRI TPEQHVAFSR RFGPLQIHVL RQFQLPGHPE VLIVSNIREN GQPIGLGDAG HFWHSDLSYK EKPSLGSLLH AQELPSEGGD TLFANMHLAW DTLPEHLRKA VEGRTAEHTY LAKYAELQKR SPWRPNLSAE QVAEVKPVVH PVVRTHPETG RKALFVSEHF TTHIVGLPED ESRDLLGALF VHSVRDEHIY RHQWAEHDLV FWDNRSLMHL AAGTPDHLRR KLYRTXXXGD APF
Validated NT Sequence
ctcacttaga acggcgcgtc gccttnnnnn nnngtgcggt acagcttgcg gcgcagatga tcgggcgtgc ccgcggcgag gtgcatcaac gagcggttgt cccagaacac gagatcgtgc tcagcccact gatgacgata gatgtgctcg tcgcgcacgc tgtgcacaaa cagcgcgcca agcagatcgc ggctttcgtc ctcgggaaga ccgacaatat gcgtagtgaa atgctcgctg acgaaaagcg ccttgcggcc cgtttccgga tgcgtgcgca cgacgggatg cacgacgggt ttcacttcgg cgacctgctc agccgagaga ttcggccgcc acgggctgcg cttttgcagc tccgcatatt tcgcgaggta cgtatgctcc gccgtgcgtc cttcgacggc cttgcgcaga tgctcgggca atgtgtccca cgccaggtgc atgttcgcga acagcgtgtc accgccttcc gacggcaact cttgcgcgtg aagcagcgag ccgagactcg gtttctcttt gtacgacaga tccgaatgcc agaagtgacc ggcatcgccg agtccgatcg gctgaccgtt ctcgcgaatg ttcgatacga tcagcacttc gggatggccg ggcaactgga actgacgcag cacgtggatt tgcagcggcc cgaagcggcg gctgaacgcg acatgctgtt cgggcgtaat ccgctgctcg cgaaacacga gcacgtgata gtcgagatgc gcgcgatgaa tacgcgcgaa atcgtcggcg gaaagcggct cattcagatc gaggccgagc acttcggcac cgacgggtgc atcaaacgca acgatgtcga atgtctgaag cgcgccggac gattgcacgc gcgncgccgc gtggcgatgg ggtgaaacgg tggtggtcac catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMT TTVSPHRHAA PRVQSSGALQ TFDIVAFDAP VGAEVLGLDL NEPLSADDFA RIHRAHLDYH VLVFREQRIT PEQHVAFSRR FGPLQIHVLR QFQLPGHPEV LIVSNIRENG QPIGLGDAGH FWHSDLSYKE KPSLGSLLHA QELPSEGGDT LFANMHLAWD TLPEHLRKAV EGRTAEHTYL AKYAELQKRS PWRPNLSAEQ VAEVKPVVHP VVRTHPETGR KALFVSEHFT THIVGLPEDE SRDLLGALFV HSVRDEHIYR HQWAEHDLVF WDNRSLMHLA AGTPDHLRRK LYRTTIEGDA PF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacca ccaccgtttc accccatcgc cacgcggcgc cgcgcgtgca atcgtccggc gcgcttcaga cattcgacat cgttgcgttt gatgcacccg tcggtgccga agtgctcggc ctcgatctga atgagccgct ttccgccgac gatttcgcgc gtattcatcg cgcgcatctc gactatcacg tgctcgtgtt tcgcgagcag cggattacgc ccgaacagca tgtcgcgttc agccgccgct tcgggccgct gcaaatccac gtgctgcgtc agttccagtt gcccggccat cccgaagtgc tgatcgtatc gaacattcgc gagaacggtc agccgatcgg actcggcgat gccggtcact tctggcattc ggatctgtcg tacaaagaga aaccgagtct cggctcgctg cttcacgcgc aagagttgcc gtcggaaggc ggtgacacgc tgttcgcgaa catgcacctg gcgtgggaca cattgcccga gcatctgcgc aaggccgtcg aaggacgcac ggcggagcat acgtacctcg cgaaatatgc ggagctgcaa aagcgcagcc cgtggcggcc gaatctctcg gctgagcagg tcgccgaagt gaaacccgtc gtgcatcccg tcgtgcgcac gcatccggaa acgggccgca aggcgctttt cgtcagcgag catttcacta cgcatattgt cggtcttccc gaggacgaaa gccgcgatct gcttggcgcg ctgtttgtgc acagcgtgcg cgacgagcac atctatcgtc atcagtgggc tgagcacgat ctcgtgttct gggacaaccg ctcgttgatg cacctcgccg cgggcacgcc cgatcatctg cgccgcaagc tgtaccgcac gacgatcgaa ggcgacgcgc cgttctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat