BuphA.00020.z

Aldehyde Dehydrogenase

CENTER ID: BuphA.00020.z
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00020.z.B1.GE39388 Full length( BuphA.00020.z ) 1 492
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.6932
RefSeq: YP_001862668.1
UniProt: B2JSR9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRFDPATIN VRSGHFIGGE YVDVHGPRIE VARPSDGAVY ASVPIADAEL VDRAVQNAWT AFRTSGWARI APRERARVLR RFARLVAADV HTLAPLEALG STRPIRDAFN WDVPFTAEGI RFYAEFADKI GGDVAATDHD HLGMTIAEPY GVIGAIAPWN FPLVMASWKI APALAAGNAV VLKPSEMTPF SALRLAELAV EAGVPPGIFN VVQGDGRTTG DALVRHPRMA KVTFTGSTRT GAAIMAACAE TGTKPVTLEL GGKSPQIVFA DAPRLDDVAA RIAGAITGNA GQVCVAGSRL LVERPIADEL AERIQRLFSE PRAGATWSPD TTLPPIISEP QAARIEAIVG RSIASGASLR CGGHRADAAT PGAFYAPTLL THVTPQTDAV RDEIFGPVLT LQTFDTEDEA LALAAHPDYG LAAGVHTADL GRALRMVRGI EAGTVWVNRY GRTSDFMIPT GGYKRSGIGK DLGRQAYEAN LRFKSVLIDA RV
NT Sequence
ATGGACCGAT TCGATCCCGC GACGATCAAC GTGCGCAGCG GACATTTTAT CGGCGGCGAA TACGTCGATG TGCACGGACC GCGCATCGAG GTCGCGCGTC CGTCGGACGG CGCTGTCTAC GCGTCGGTGC CCATCGCCGA TGCCGAACTT GTCGACCGCG CGGTGCAGAA CGCATGGACG GCTTTTCGGA CGAGCGGCTG GGCGCGCATC GCGCCGCGCG AACGGGCGCG CGTACTGCGC CGCTTCGCCC GTCTCGTTGC CGCCGATGTC CACACGCTAG CGCCGCTAGA AGCGCTCGGC TCGACGCGGC CCATCCGCGA CGCCTTCAAC TGGGACGTGC CGTTCACGGC GGAAGGCATC CGCTTCTATG CGGAGTTCGC CGACAAGATC GGCGGCGACG TCGCCGCGAC CGATCACGAT CATCTCGGCA TGACGATCGC CGAACCGTAT GGCGTGATCG GCGCGATCGC GCCGTGGAAT TTCCCGCTTG TGATGGCGTC GTGGAAGATC GCGCCTGCGC TTGCGGCGGG CAACGCCGTC GTGCTGAAGC CTTCGGAGAT GACGCCGTTT TCCGCATTGC GGCTGGCCGA ACTCGCCGTC GAAGCGGGCG TGCCGCCCGG CATCTTCAAC GTCGTGCAAG GAGACGGCCG CACCACGGGC GATGCGCTCG TGCGTCACCC GCGCATGGCG AAGGTGACCT TCACGGGATC GACGCGCACG GGCGCGGCGA TCATGGCGGC GTGCGCCGAA ACGGGCACCA AGCCCGTCAC GCTCGAACTG GGCGGCAAGA GCCCCCAGAT CGTCTTCGCC GATGCGCCGC GTCTCGACGA CGTGGCGGCT CGCATCGCCG GCGCGATTAC GGGCAATGCG GGGCAGGTCT GCGTCGCGGG GTCGCGGCTG CTGGTCGAGC GGCCGATCGC GGACGAACTT GCCGAACGCA TCCAGCGCCT TTTCAGCGAA CCGAGGGCGG GCGCAACCTG GTCGCCCGAT ACGACGCTGC CGCCGATCAT CTCGGAGCCG CAGGCGGCGC GGATCGAGGC GATCGTCGGG CGCAGCATCG CGAGCGGCGC GAGCTTGCGC TGCGGCGGCC ATCGCGCGGA TGCGGCGACG CCGGGCGCCT TCTACGCGCC GACGCTGCTC ACGCACGTCA CGCCGCAGAC AGACGCTGTG CGTGACGAGA TCTTCGGCCC CGTGCTGACC TTGCAGACCT TCGACACGGA AGACGAAGCG CTCGCGCTAG CCGCGCATCC CGACTACGGC CTTGCCGCCG GCGTGCATAC GGCCGATCTG GGCCGCGCGC TGCGCATGGT GCGCGGCATC GAGGCGGGTA CGGTCTGGGT GAACCGCTAC GGACGCACCA GCGACTTCAT GATTCCGACG GGCGGCTACA AGCGTTCGGG CATCGGCAAG GATCTTGGCC GGCAGGCTTA CGAAGCGAAC CTGCGCTTCA AGAGCGTGCT GATCGACGCG CGCGTC
Details for BuphA.00020.z.B1.GE39388
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMD RFDPATINVR SGHFIGGEYV DVHGPRIEVA RPSDGAVYAS VPIADAELVD RAVQNAWTAF RTSGWARIAP RERARVLRRF ARLVAADVHT LAPLEALGST RPIRDAFNWD VPFTAEGIRF YAEFADKIGG DVAATDHDHL GMTIAEPYGV IGAIAPWNFP LVMASWKIAP ALAAGNAVVL KPSEMTPFSA LRLAELAVEA GVPPGIFNVV QGDGRTTGDA LVRHPRMAKV TFTGSTRTGA AIMAACAETG TKPVTLELGG KSPQIVFADA PRLDDVAARI AGAITGNAGQ VCVAGSRLLV ERPIADELAE RIQRLFSEPR AGATWSPDTT LPPIISEPQA ARIEAIVGRS IASGASLRCG GHRADAATPG AFYAPTLLTH VTPQTDAVRD EIFGPVLTLQ TFDTEDEALA LAAHPDYGLA AGVHTADLGR ALRMVRGIEA GTVWVNRYGR TSDFMIPTGG YKRSGIGKDL GRQAYEANLR FK
Validated NT Sequence
tcttgaagcg caggttcgct tcgtaagcct gccggccaag atccttgccg atgcccgaac gcttgtagcc gcccgtcgga atcatgaagt cgctggtgcg tccgtagcgg ttcacccaga ccgtacccgc ctcgatgccg cgcaccatgc gcagcgcgcg gcccagatcg gccgtatgca cgccggcggc aaggccgtag tcgggatgcg cggctagcgc gagcgcttcg tcttccgtgt cgaaggtctg caaggtcagc acggggccga agatctcgtc acgcacagcg tctgtctgcg gcgtgacgtg cgtgagcagc gtcggcgcgt agaaggcgcc cggcgtcgcc gcatccgcgc gatggccgcc gcagcgcaag ctcgcgccgc tcgcgatgct gcgcccgacg atcgcctcga tccgcgccgc ctgcggctcc gagatgatcg gcggcagcgt cgtatcgggc gaccaggttg cgcccgccct cggttcgctg aaaaggcgct ggatgcgttc ggcaagttcg tccgcgatcg gccgctcgac cagcagccgc gaccccgcga cgcagacctg ccccgcattg cccgtaatcg cgccggcgat gcgagccgcc acgtcgtcga gacgcggcgc atcggcgaag acgatctggg ggctcttgcc gcccagttcg agcgtgacgg gcttggtgcc cgtttcggcg cacgccgcca tgatcgccgc gcccgtgcgc gtcgatcccg tgaaggtcac cttcgccatg cgcgggtgac gcacgagcgc atcgcccgtg gtgcggccgt ctccttgcac gacgttgaag atgccgggcg gcacgcccgc ttcgacggcg agttcggcca gccgcaatgc ggaaaacggc gtcatctccg aaggcttcag cacgacggcg ttgcccgccg caagcgcagg cgcgatcttc cacgacgcca tcacaagcgg gaaattccac ggcgcgatcg cgccgatcac gccatacggt tcggcgatcg tcatgccgag atgatcgtga tcggtcgcgg cgacgtcgcc gccgatcttg tcggcgaact ccgcatagaa gcggatgcct tccgccgtga acggcacgtc ccagttgaag gcgtcgcgga tgggccgcgt cgagccgagc gcttctagcg gcgctagcgt gtggacatcg gcggcaacga gacgggcgaa gcggcgcagt acgcgcgccc gttcgcgcgg cgcgatgcgc gcccagccgc tcgtccgaaa agccgtccat gcgttctgca ccgcgcggtc gacaagttcg gcatcggcga tgggcaccga cgcgtagaca gcgccgtccg acggacgcgc gacctcgatg cgcggtccgt gcacatcgac gtattcgccg ccgataaaat gtccgctgcg cacgttgatc gtcgcgggat cgaatcggtc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMD RFDPATINVR SGHFIGGEYV DVHGPRIEVA RPSDGAVYAS VPIADAELVD RAVQNAWTAF RTSGWARIAP RERARVLRRF ARLVAADVHT LAPLEALGST RPIRDAFNWD VPFTAEGIRF YAEFADKIGG DVAATDHDHL GMTIAEPYGV IGAIAPWNFP LVMASWKIAP ALAAGNAVVL KPSEMTPFSA LRLAELAVEA GVPPGIFNVV QGDGRTTGDA LVRHPRMAKV TFTGSTRTGA AIMAACAETG TKPVTLELGG KSPQIVFADA PRLDDVAARI AGAITGNAGQ VCVAGSRLLV ERPIADELAE RIQRLFSEPR AGATWSPDTT LPPIISEPQA ARIEAIVGRS IASGASLRCG GHRADAATPG AFYAPTLLTH VTPQTDAVRD EIFGPVLTLQ TFDTEDEALA LAAHPDYGLA AGVHTADLGR ALRMVRGIEA GTVWVNRYGR TSDFMIPTGG YKRSGIGKDL GRQAYEANLR FKSVLIDARV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacc gattcgatcc cgcgacgatc aacgtgcgca gcggacattt tatcggcggc gaatacgtcg atgtgcacgg accgcgcatc gaggtcgcgc gtccgtcgga cggcgctgtc tacgcgtcgg tgcccatcgc cgatgccgaa cttgtcgacc gcgcggtgca gaacgcatgg acggcttttc ggacgagcgg ctgggcgcgc atcgcgccgc gcgaacgggc gcgcgtactg cgccgcttcg cccgtctcgt tgccgccgat gtccacacgc tagcgccgct agaagcgctc ggctcgacgc ggcccatccg cgacgccttc aactgggacg tgccgttcac ggcggaaggc atccgcttct atgcggagtt cgccgacaag atcggcggcg acgtcgccgc gaccgatcac gatcatctcg gcatgacgat cgccgaaccg tatggcgtga tcggcgcgat cgcgccgtgg aatttcccgc ttgtgatggc gtcgtggaag atcgcgcctg cgcttgcggc gggcaacgcc gtcgtgctga agccttcgga gatgacgccg ttttccgcat tgcggctggc cgaactcgcc gtcgaagcgg gcgtgccgcc cggcatcttc aacgtcgtgc aaggagacgg ccgcaccacg ggcgatgcgc tcgtgcgtca cccgcgcatg gcgaaggtga ccttcacggg atcgacgcgc acgggcgcgg cgatcatggc ggcgtgcgcc gaaacgggca ccaagcccgt cacgctcgaa ctgggcggca agagccccca gatcgtcttc gccgatgcgc cgcgtctcga cgacgtggcg gctcgcatcg ccggcgcgat tacgggcaat gcggggcagg tctgcgtcgc ggggtcgcgg ctgctggtcg agcggccgat cgcggacgaa cttgccgaac gcatccagcg ccttttcagc gaaccgaggg cgggcgcaac ctggtcgccc gatacgacgc tgccgccgat catctcggag ccgcaggcgg cgcggatcga ggcgatcgtc gggcgcagca tcgcgagcgg cgcgagcttg cgctgcggcg gccatcgcgc ggatgcggcg acgccgggcg ccttctacgc gccgacgctg ctcacgcacg tcacgccgca gacagacgct gtgcgtgacg agatcttcgg ccccgtgctg accttgcaga ccttcgacac ggaagacgaa gcgctcgcgc tagccgcgca tcccgactac ggccttgccg ccggcgtgca tacggccgat ctgggccgcg cgctgcgcat ggtgcgcggc atcgaggcgg gtacggtctg ggtgaaccgc tacggacgca ccagcgactt catgattccg acgggcggct acaagcgttc gggcatcggc aaggatcttg gccggcaggc ttacgaagcg aacctgcgct tcaagagcgt gctgatcgac gcgcgcgtct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat