BuphA.00020.b

Aldehyde Dehydrogenase

CENTER ID: BuphA.00020.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00020.b.B1.GE39387 Full length( BuphA.00020.b ) 1 478
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00020.b.B1.PS02559 Full length( BuphA.00020.b ) 1 478
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126638
PATRIC ID: fig|391038.7.peg.2218
RefSeq: YP_001858307.1
UniProt: B2JE53
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLQKSYPYYL ANEPVAANTD LEVTDKFSGE VATRVAMADA KAIDQAIGAA VAALPALRTF PPFKRQAVLE HCVKRFRERA DELAMALCIE AGKPINDSRG EVARLIDTFK VAAEESVRID GEIVNLEISP RAKGYHAYVK RVPIGPCSFI SPFNFPLNLA AHKVAPALAA GVPFVLKPAS RTPVGALIIG EVLAQTDLPK GAFSILPAHR DGADLFTTDE RFKLLSFTGS PAVGWDLKAK AGKKKVILEL GGNAAAIVDS DQRDKLDYVV ERLAFGAFYQ SGQSCIGVQR ILAHASLYDA LRDKLIAKTK SLKMGDPKDE KTFVGPMISE SESRRLSGWM DDAVKAGAKI VAGGKVDGAM FEATLLESVG HDTDLYRKEA FGPVAILEKF DDFASALAAV NDSDFGLQAG VFTDSLAHAH QAWDELEVGG VVINDVPSFR VDNMPYGGVK DSGLGREGIR YAIEDMTEPR LMVMRETW
NT Sequence
ATGTTGCAGA AGAGTTATCC CTACTATCTC GCGAACGAAC CCGTCGCCGC GAACACCGAT CTCGAGGTCA CCGACAAATT CAGCGGCGAA GTGGCGACGC GCGTCGCGAT GGCCGATGCG AAGGCGATCG ATCAAGCCAT CGGCGCGGCC GTCGCCGCGT TGCCCGCGCT GCGCACGTTC CCGCCGTTCA AGCGTCAGGC CGTGCTCGAA CATTGCGTGA AGCGCTTTCG CGAGCGCGCC GACGAACTGG CGATGGCACT GTGCATCGAA GCCGGCAAGC CGATCAACGA TTCGCGCGGC GAGGTCGCGC GTCTGATCGA TACGTTCAAG GTCGCGGCGG AAGAATCGGT GCGCATCGAC GGCGAAATCG TCAACCTGGA AATCTCGCCG CGCGCAAAGG GCTATCACGC GTACGTGAAG CGGGTGCCCA TCGGACCGTG CTCGTTCATC TCGCCGTTCA ACTTTCCGCT GAATCTGGCC GCGCACAAGG TGGCGCCCGC GCTGGCGGCA GGCGTGCCGT TCGTGCTGAA ACCGGCGAGC CGCACGCCCG TCGGCGCGCT GATCATCGGC GAGGTGCTCG CGCAGACCGA TCTGCCGAAG GGTGCGTTCT CGATCCTGCC CGCGCATCGC GACGGCGCCG ATCTGTTCAC GACGGACGAG CGCTTCAAGC TGCTGTCGTT CACGGGCTCG CCTGCCGTCG GCTGGGACCT GAAGGCGAAG GCGGGCAAGA AGAAGGTGAT TCTGGAGCTG GGCGGCAACG CGGCCGCCAT CGTCGACAGC GATCAGCGCG ACAAGCTCGA CTACGTTGTG GAGCGCCTTG CGTTCGGCGC GTTTTATCAG TCGGGGCAAA GCTGCATCGG CGTGCAGCGC ATTCTCGCGC ATGCGTCGCT GTACGATGCG CTGCGCGACA AGCTGATCGC GAAAACGAAG TCGCTGAAGA TGGGCGATCC GAAGGACGAG AAGACTTTCG TCGGGCCGAT GATCTCCGAA TCGGAATCGC GCCGTCTTTC AGGCTGGATG GACGACGCAG TGAAGGCGGG CGCGAAGATC GTCGCAGGCG GCAAGGTGGA CGGCGCGATG TTCGAGGCGA CGTTGCTCGA GAGCGTCGGG CATGACACCG ATCTGTATCG CAAGGAGGCG TTCGGGCCGG TGGCGATCCT CGAAAAATTC GACGACTTCG CGAGCGCGCT CGCGGCCGTC AACGACAGCG ACTTCGGCTT GCAGGCGGGC GTGTTCACGG ATTCCCTGGC GCACGCGCAT CAGGCGTGGG ATGAACTCGA AGTGGGCGGC GTGGTCATCA ACGATGTGCC ATCGTTCCGC GTCGACAACA TGCCGTATGG CGGCGTGAAG GACTCGGGCC TGGGGCGCGA AGGGATCCGC TATGCGATCG AGGACATGAC GGAACCGCGG CTGATGGTGA TGCGCGAAAC GTGG
Details for BuphA.00020.b.B1.GE39387
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML QKSYPYYLAN EPVAANTDLE VTDKFSGEVA TRVAMADAKA IDQAIGAAVA ALPALRTFPP FKRQAVLEHC VKRFRERADE LAMALCIEAG KPINDSRGEV ARLIDTFKVA AEESVRIDGE IVNLEISPRA KGYHAYVKRV PIGPCSFISP FNFPLNLAAH KVAPALAAGV PFVLKPASRT PVGALIIGEV LAQTDLPKGA FSILPAHRDG ADLFTTDERF KLLSFTGSPA VGWDLKAKAG KKKVILELGG NAAAIVDSDQ RDKLDYVVER LAFGAFYQSG QSCIGVQRIL AHASLYDALR DKLIAKTKSL KMGDPKDEKT FVGPMISESE SRRLSGWMDD AVKAGAKIVA GGKVDGAMFE ATLLESVGHD TDLYRKEAFG PVAILEKFDD FASALAAVND SDFGLQAGVF TDSLAHAHQA WDELEVGGVV INDVPSFRVD NMPYGGVKDS GLGREGIRYA IEDMTEP
Validated NT Sequence
cgcggttccg tcatgtcctc gatcgcatag cggatccctt cgcgccccag gcccgagtcc ttcacgccgc catacggcat gttgtcgacg cggaacgatg gcacatcgtt gatgaccacg ccgcccactt cgagttcatc ccacgcctga tgcgcgtgcg ccagggaatc cgtgaacacg cccgcctgca agccgaagtc gctgtcgttg acggccgcga gcgcgctcgc gaagtcgtcg aatttttcga ggatcgccac cggcccgaac gcctccttgc gatacagatc ggtgtcatgc ccgacgctct cgagcaacgt cgcctcgaac atcgcgccgt ccaccttgcc gcctgcgacg atcttcgcgc ccgccttcac tgcgtcgtcc atccagcctg aaagacggcg cgattccgat tcggagatca tcggcccgac gaaagtcttc tcgtccttcg gatcgcccat cttcagcgac ttcgttttcg cgatcagctt gtcgcgcagc gcatcgtaca gcgacgcatg cgcgagaatg cgctgcacgc cgatgcagct ttgccccgac tgataaaacg cgccgaacgc aaggcgctcc acaacgtagt cgagcttgtc gcgctgatcg ctgtcgacga tggcggccgc gttgccgccc agctccagaa tcaccttctt cttgcccgcc ttcgccttca ggtcccagcc gacggcaggc gagcccgtga acgacagcag cttgaagcgc tcgtccgtcg tgaacagatc ggcgccgtcg cgatgcgcgg gcaggatcga gaacgcaccc ttcggcagat cggtctgcgc gagcacctcg ccgatgatca gcgcgccgac gggcgtgcgg ctcgccggtt tcagcacgaa cggcacgcct gccgccagcg cgggcgccac cttgtgcgcg gccagattca gcggaaagtt gaacggcgag atgaacgagc acggtccgat gggcacccgc ttcacgtacg cgtgatagcc ctttgcgcgc ggcgagattt ccaggttgac gatttcgccg tcgatgcgca ccgattcttc cgccgcgacc ttgaacgtat cgatcagacg cgcgacctcg ccgcgcgaat cgttgatcgg cttgccggct tcgatgcaca gtgccatcgc cagttcgtcg gcgcgctcgc gaaagcgctt cacgcaatgt tcgagcacgg cctgacgctt gaacggcggg aacgtgcgca gcgcgggcaa cgcggcgacg gccgcgccga tggcttgatc gatcgccttc gcatcggcca tcgcgacgcg cgtcgccact tcgccgctga atttgtcggt gacctcgaga tcggtgttcg cggcgacggg ttcgttcgcg agatagtagg gataactctt ctgcaacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHML QKSYPYYLAN EPVAANTDLE VTDKFSGEVA TRVAMADAKA IDQAIGAAVA ALPALRTFPP FKRQAVLEHC VKRFRERADE LAMALCIEAG KPINDSRGEV ARLIDTFKVA AEESVRIDGE IVNLEISPRA KGYHAYVKRV PIGPCSFISP FNFPLNLAAH KVAPALAAGV PFVLKPASRT PVGALIIGEV LAQTDLPKGA FSILPAHRDG ADLFTTDERF KLLSFTGSPA VGWDLKAKAG KKKVILELGG NAAAIVDSDQ RDKLDYVVER LAFGAFYQSG QSCIGVQRIL AHASLYDALR DKLIAKTKSL KMGDPKDEKT FVGPMISESE SRRLSGWMDD AVKAGAKIVA GGKVDGAMFE ATLLESVGHD TDLYRKEAFG PVAILEKFDD FASALAAVND SDFGLQAGVF TDSLAHAHQA WDELEVGGVV INDVPSFRVD NMPYGGVKDS GLGREGIRYA IEDMTEPRLM VMRETW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc agaagagtta tccctactat ctcgcgaacg aacccgtcgc cgcgaacacc gatctcgagg tcaccgacaa attcagcggc gaagtggcga cgcgcgtcgc gatggccgat gcgaaggcga tcgatcaagc catcggcgcg gccgtcgccg cgttgcccgc gctgcgcacg ttcccgccgt tcaagcgtca ggccgtgctc gaacattgcg tgaagcgctt tcgcgagcgc gccgacgaac tggcgatggc actgtgcatc gaagccggca agccgatcaa cgattcgcgc ggcgaggtcg cgcgtctgat cgatacgttc aaggtcgcgg cggaagaatc ggtgcgcatc gacggcgaaa tcgtcaacct ggaaatctcg ccgcgcgcaa agggctatca cgcgtacgtg aagcgggtgc ccatcggacc gtgctcgttc atctcgccgt tcaactttcc gctgaatctg gccgcgcaca aggtggcgcc cgcgctggcg gcaggcgtgc cgttcgtgct gaaaccggcg agccgcacgc ccgtcggcgc gctgatcatc ggcgaggtgc tcgcgcagac cgatctgccg aagggtgcgt tctcgatcct gcccgcgcat cgcgacggcg ccgatctgtt cacgacggac gagcgcttca agctgctgtc gttcacgggc tcgcctgccg tcggctggga cctgaaggcg aaggcgggca agaagaaggt gattctggag ctgggcggca acgcggccgc catcgtcgac agcgatcagc gcgacaagct cgactacgtt gtggagcgcc ttgcgttcgg cgcgttttat cagtcggggc aaagctgcat cggcgtgcag cgcattctcg cgcatgcgtc gctgtacgat gcgctgcgcg acaagctgat cgcgaaaacg aagtcgctga agatgggcga tccgaaggac gagaagactt tcgtcgggcc gatgatctcc gaatcggaat cgcgccgtct ttcaggctgg atggacgacg cagtgaaggc gggcgcgaag atcgtcgcag gcggcaaggt ggacggcgcg atgttcgagg cgacgttgct cgagagcgtc gggcatgaca ccgatctgta tcgcaaggag gcgttcgggc cggtggcgat cctcgaaaaa ttcgacgact tcgcgagcgc gctcgcggcc gtcaacgaca gcgacttcgg cttgcaggcg ggcgtgttca cggattccct ggcgcacgcg catcaggcgt gggatgaact cgaagtgggc ggcgtggtca tcaacgatgt gccatcgttc cgcgtcgaca acatgccgta tggcggcgtg aaggactcgg gcctggggcg cgaagggatc cgctatgcga tcgaggacat gacggaaccg cggctgatgg tgatgcgcga aacgtggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BuphA.00020.b.B1.PS02559
PURIFICATION DATe: 12/22/2015
CONCENTRATION: 53.61mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML QKSYPYYLAN EPVAANTDLE VTDKFSGEVA TRVAMADAKA IDQAIGAAVA ALPALRTFPP FKRQAVLEHC VKRFRERADE LAMALCIEAG KPINDSRGEV ARLIDTFKVA AEESVRIDGE IVNLEISPRA KGYHAYVKRV PIGPCSFISP FNFPLNLAAH KVAPALAAGV PFVLKPASRT PVGALIIGEV LAQTDLPKGA FSILPAHRDG ADLFTTDERF KLLSFTGSPA VGWDLKAKAG KKKVILELGG NAAAIVDSDQ RDKLDYVVER LAFGAFYQSG QSCIGVQRIL AHASLYDALR DKLIAKTKSL KMGDPKDEKT FVGPMISESE SRRLSGWMDD AVKAGAKIVA GGKVDGAMFE ATLLESVGHD TDLYRKEAFG PVAILEKFDD FASALAAVND SDFGLQAGVF TDSLAHAHQA WDELEVGGVV INDVPSFRVD NMPYGGVKDS GLGREGIRYA IEDMTEP
Validated NT Sequence
cgcggttccg tcatgtcctc gatcgcatag cggatccctt cgcgccccag gcccgagtcc ttcacgccgc catacggcat gttgtcgacg cggaacgatg gcacatcgtt gatgaccacg ccgcccactt cgagttcatc ccacgcctga tgcgcgtgcg ccagggaatc cgtgaacacg cccgcctgca agccgaagtc gctgtcgttg acggccgcga gcgcgctcgc gaagtcgtcg aatttttcga ggatcgccac cggcccgaac gcctccttgc gatacagatc ggtgtcatgc ccgacgctct cgagcaacgt cgcctcgaac atcgcgccgt ccaccttgcc gcctgcgacg atcttcgcgc ccgccttcac tgcgtcgtcc atccagcctg aaagacggcg cgattccgat tcggagatca tcggcccgac gaaagtcttc tcgtccttcg gatcgcccat cttcagcgac ttcgttttcg cgatcagctt gtcgcgcagc gcatcgtaca gcgacgcatg cgcgagaatg cgctgcacgc cgatgcagct ttgccccgac tgataaaacg cgccgaacgc aaggcgctcc acaacgtagt cgagcttgtc gcgctgatcg ctgtcgacga tggcggccgc gttgccgccc agctccagaa tcaccttctt cttgcccgcc ttcgccttca ggtcccagcc gacggcaggc gagcccgtga acgacagcag cttgaagcgc tcgtccgtcg tgaacagatc ggcgccgtcg cgatgcgcgg gcaggatcga gaacgcaccc ttcggcagat cggtctgcgc gagcacctcg ccgatgatca gcgcgccgac gggcgtgcgg ctcgccggtt tcagcacgaa cggcacgcct gccgccagcg cgggcgccac cttgtgcgcg gccagattca gcggaaagtt gaacggcgag atgaacgagc acggtccgat gggcacccgc ttcacgtacg cgtgatagcc ctttgcgcgc ggcgagattt ccaggttgac gatttcgccg tcgatgcgca ccgattcttc cgccgcgacc ttgaacgtat cgatcagacg cgcgacctcg ccgcgcgaat cgttgatcgg cttgccggct tcgatgcaca gtgccatcgc cagttcgtcg gcgcgctcgc gaaagcgctt cacgcaatgt tcgagcacgg cctgacgctt gaacggcggg aacgtgcgca gcgcgggcaa cgcggcgacg gccgcgccga tggcttgatc gatcgccttc gcatcggcca tcgcgacgcg cgtcgccact tcgccgctga atttgtcggt gacctcgaga tcggtgttcg cggcgacggg ttcgttcgcg agatagtagg gataactctt ctgcaacata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHML QKSYPYYLAN EPVAANTDLE VTDKFSGEVA TRVAMADAKA IDQAIGAAVA ALPALRTFPP FKRQAVLEHC VKRFRERADE LAMALCIEAG KPINDSRGEV ARLIDTFKVA AEESVRIDGE IVNLEISPRA KGYHAYVKRV PIGPCSFISP FNFPLNLAAH KVAPALAAGV PFVLKPASRT PVGALIIGEV LAQTDLPKGA FSILPAHRDG ADLFTTDERF KLLSFTGSPA VGWDLKAKAG KKKVILELGG NAAAIVDSDQ RDKLDYVVER LAFGAFYQSG QSCIGVQRIL AHASLYDALR DKLIAKTKSL KMGDPKDEKT FVGPMISESE SRRLSGWMDD AVKAGAKIVA GGKVDGAMFE ATLLESVGHD TDLYRKEAFG PVAILEKFDD FASALAAVND SDFGLQAGVF TDSLAHAHQA WDELEVGGVV INDVPSFRVD NMPYGGVKDS GLGREGIRYA IEDMTEPRLM VMRETW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGC AGAAGAGTTA TCCCTACTAT CTCGCGAACG AACCCGTCGC CGCGAACACC GATCTCGAGG TCACCGACAA ATTCAGCGGC GAAGTGGCGA CGCGCGTCGC GATGGCCGAT GCGAAGGCGA TCGATCAAGC CATCGGCGCG GCCGTCGCCG CGTTGCCCGC GCTGCGCACG TTCCCGCCGT TCAAGCGTCA GGCCGTGCTC GAACATTGCG TGAAGCGCTT TCGCGAGCGC GCCGACGAAC TGGCGATGGC ACTGTGCATC GAAGCCGGCA AGCCGATCAA CGATTCGCGC GGCGAGGTCG CGCGTCTGAT CGATACGTTC AAGGTCGCGG CGGAAGAATC GGTGCGCATC GACGGCGAAA TCGTCAACCT GGAAATCTCG CCGCGCGCAA AGGGCTATCA CGCGTACGTG AAGCGGGTGC CCATCGGACC GTGCTCGTTC ATCTCGCCGT TCAACTTTCC GCTGAATCTG GCCGCGCACA AGGTGGCGCC CGCGCTGGCG GCAGGCGTGC CGTTCGTGCT GAAACCGGCG AGCCGCACGC CCGTCGGCGC GCTGATCATC GGCGAGGTGC TCGCGCAGAC CGATCTGCCG AAGGGTGCGT TCTCGATCCT GCCCGCGCAT CGCGACGGCG CCGATCTGTT CACGACGGAC GAGCGCTTCA AGCTGCTGTC GTTCACGGGC TCGCCTGCCG TCGGCTGGGA CCTGAAGGCG AAGGCGGGCA AGAAGAAGGT GATTCTGGAG CTGGGCGGCA ACGCGGCCGC CATCGTCGAC AGCGATCAGC GCGACAAGCT CGACTACGTT GTGGAGCGCC TTGCGTTCGG CGCGTTTTAT CAGTCGGGGC AAAGCTGCAT CGGCGTGCAG CGCATTCTCG CGCATGCGTC GCTGTACGAT GCGCTGCGCG ACAAGCTGAT CGCGAAAACG AAGTCGCTGA AGATGGGCGA TCCGAAGGAC GAGAAGACTT TCGTCGGGCC GATGATCTCC GAATCGGAAT CGCGCCGTCT TTCAGGCTGG ATGGACGACG CAGTGAAGGC GGGCGCGAAG ATCGTCGCAG GCGGCAAGGT GGACGGCGCG ATGTTCGAGG CGACGTTGCT CGAGAGCGTC GGGCATGACA CCGATCTGTA TCGCAAGGAG GCGTTCGGGC CGGTGGCGAT CCTCGAAAAA TTCGACGACT TCGCGAGCGC GCTCGCGGCC GTCAACGACA GCGACTTCGG CTTGCAGGCG GGCGTGTTCA CGGATTCCCT GGCGCACGCG CATCAGGCGT GGGATGAACT CGAAGTGGGC GGCGTGGTCA TCAACGATGT GCCATCGTTC CGCGTCGACA ACATGCCGTA TGGCGGCGTG AAGGACTCGG GCCTGGGGCG CGAAGGGATC CGCTATGCGA TCGAGGACAT GACGGAACCG CGGCTGATGG TGATGCGCGA AACGTGGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT