BuphA.00013.a

Holliday junction ATP-dependent DNA helicase ruvB

CENTER ID: BuphA.00013.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00013.a.B1.GE40184 Full length( BuphA.00013.a ) 1 363
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00013.a.B1.PS38154 Full length( BuphA.00013.a ) 1 363

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.2714
RefSeq: YP_001858775.1
UniProt: B2JGU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGHARYNARM IEHDKLAAER IIAATPVSPN EEAFERALRP RQLDEYVGQE KVRGQLEIFI EAAKRRSESL DHVLLFGPPG LGKTTLAHII AREMGVNLRQ TSGPVLERAG DLAALLTNLE ANDVLFIDEI HRLSPVVEEI LYPALEDYQI DIMIGEGPAA RSVKLDLQPF TLVGATTRAG MLTNPLRDRF GIVARLEFYN AEELARIVSR SASLLGAQIH PEGALEIAKR SRGTPRIANR LLRRVRDYAE VKADGNITAK VADAALKMLD VDPVGFDLMD RKLLEAILHK FDGGPVGVDN LAAAIGEERD TIEDVLEPYL IQQGFLQRTP RGRVATLLTY RHFGLAAPDS ASPVRNLWDA EPS
NT Sequence
ATGGGCCACG CGCGGTACAA TGCCCGCATG ATCGAACACG ACAAACTCGC CGCCGAACGC ATCATCGCCG CCACGCCCGT GTCGCCGAAC GAGGAAGCGT TCGAGCGCGC GTTGCGCCCC CGTCAGCTGG ACGAATACGT CGGCCAGGAA AAGGTGCGCG GCCAGCTGGA AATTTTCATC GAGGCTGCCA AGCGCCGCTC GGAATCGCTG GATCACGTGC TGCTGTTCGG TCCGCCTGGC CTCGGCAAGA CCACGCTCGC GCATATCATC GCGCGCGAAA TGGGCGTGAA TCTGCGGCAG ACTTCCGGTC CCGTGCTGGA GCGCGCGGGC GATCTCGCGG CGCTTCTGAC CAACCTCGAA GCCAATGACG TTCTCTTCAT CGACGAGATT CACCGGCTGT CGCCCGTCGT CGAGGAAATC CTGTATCCGG CGCTCGAGGA TTATCAGATC GATATCATGA TCGGCGAAGG CCCCGCGGCG CGCAGTGTGA AGCTCGATCT GCAACCGTTC ACGCTGGTCG GCGCCACGAC GCGCGCGGGC ATGCTGACCA ATCCGTTGCG CGACCGCTTT GGTATTGTCG CGCGATTGGA GTTTTATAAC GCAGAAGAGC TGGCACGCAT CGTGAGCCGT TCCGCATCGC TGCTCGGCGC GCAGATCCAC CCGGAAGGTG CACTGGAAAT CGCGAAGCGC TCGCGCGGCA CGCCGCGTAT CGCGAACCGT CTGCTGCGGC GCGTGCGCGA TTACGCGGAA GTGAAGGCGG ACGGCAATAT CACCGCGAAG GTCGCGGATG CAGCGCTGAA AATGCTCGAC GTCGATCCGG TCGGTTTTGA TCTGATGGAC CGCAAGCTTC TCGAAGCGAT CTTGCACAAG TTCGATGGCG GTCCCGTGGG CGTCGACAAT CTCGCGGCGG CAATTGGCGA AGAGCGCGAC ACAATCGAAG ACGTGCTCGA GCCGTATCTG ATCCAGCAGG GCTTTCTGCA ACGCACGCCG CGTGGACGCG TCGCCACGTT GTTGACGTAT CGGCACTTCG GGCTTGCCGC ACCTGACTCG GCAAGTCCTG TTCGCAATCT GTGGGATGCC GAGCCGAGC
Details for BuphA.00013.a.B1.GE40184
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG HARYNARMIE HDKLAAERII AATPVSPNEE AFERALRPRQ LDEYVGQEKV RGQLEIFIEA AKRRSESLDH VLLFGPPGLG KTTLAHIIAR EMGVNLRQTS GPVLERAGDL AALLTNLEAN DVLFIDEIHR LSPVVEEILY PALEDYQIDI MIGEGPAARS VKLDLQPFTL VGATTRAGML TNPLRDRFGI VARLEFYNAE ELARIVSRSA SLLGAQIHPE GALEIAKRSR GTPRIANRLL RRVRDYAEVK ADGNITAKVA DAALKMLDVD PVGFDLMDRK LLEAILHKFD GGPVGVDNLA AAIGEERDTI EDVLEPYLIQ QGFLQRTPRG RVATLLTYRH FGLAAPDSAS PVX
Validated NT Sequence
ttnngaacag gacttgccga gtcaggtgcg gcaagcccga agtgccgata cgtcaacaac gtggcgacgc gtccacgcgg cgtgcgttgc agaaagccct gctggatcag atacggctcg agcacgtctt cgattgtgtc gcgctcttcg ccaattgccg ccgcgagatt gtcgacgccc acgggaccgc catcgaactt gtgcaagatc gcttcgagaa gcttgcggtc catcagatca aaaccgaccg gatcgacgtc gagcattttc agcgctgcat ccgcgacctt cgcggtgata ttgccgtccg ccttcacttc cgcgtaatcg cgcacgcgcc gcagcagacg gttcgcgata cgcggcgtgc cgcgcgagcg cttcgcgatt tccagtgcac cttccgggtg gatctgcgcg ccgagcagcg atgcggaacg gctcacgatg cgtgccagct cttctgcgtt ataaaactcc aatcgcgcga caataccaaa gcggtcgcgc aacggattgg tcagcatgcc cgcgcgcgtc gtggcgccga ccagcgtgaa cggttgcaga tcgagcttca cactgcgcgc cgcggggcct tcgccgatca tgatatcgat ctgataatcc tcgagcgccg gatacaggat ttcctcgacg acgggcgaca gccggtgaat ctcgtcgatg aagagaacgt cattggcttc gaggttggtc agaagcgccg cgagatcgcc cgcgcgctcc agcacgggac cggaagtctg ccgcagattc acgcccattt cgcgcgcgat gatatgcgcg agcgtggtct tgccgaggcc aggcggaccg aacagcagca cgtgatccag cgattccgag cggcgcttgg cagcctcgat gaaaatttcc agctggccgc gcaccttttc ctggccgacg tattcgtcca gctgacgggg gcgcaacgcg cgctcgaacg cttcctcgtt cggcgacacg ggcgtggcgg cgatgatgcg ttcggcggcg agtttgtcgt gttcgatcat gcgggcattg taccgcgcgt ggcccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMG HARYNARMIE HDKLAAERII AATPVSPNEE AFERALRPRQ LDEYVGQEKV RGQLEIFIEA AKRRSESLDH VLLFGPPGLG KTTLAHIIAR EMGVNLRQTS GPVLERAGDL AALLTNLEAN DVLFIDEIHR LSPVVEEILY PALEDYQIDI MIGEGPAARS VKLDLQPFTL VGATTRAGML TNPLRDRFGI VARLEFYNAE ELARIVSRSA SLLGAQIHPE GALEIAKRSR GTPRIANRLL RRVRDYAEVK ADGNITAKVA DAALKMLDVD PVGFDLMDRK LLEAILHKFD GGPVGVDNLA AAIGEERDTI EDVLEPYLIQ QGFLQRTPRG RVATLLTYRH FGLAAPDSAS PVRNLWDAEP S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcc acgcgcggta caatgcccgc atgatcgaac acgacaaact cgccgccgaa cgcatcatcg ccgccacgcc cgtgtcgccg aacgaggaag cgttcgagcg cgcgttgcgc ccccgtcagc tggacgaata cgtcggccag gaaaaggtgc gcggccagct ggaaattttc atcgaggctg ccaagcgccg ctcggaatcg ctggatcacg tgctgctgtt cggtccgcct ggcctcggca agaccacgct cgcgcatatc atcgcgcgcg aaatgggcgt gaatctgcgg cagacttccg gtcccgtgct ggagcgcgcg ggcgatctcg cggcgcttct gaccaacctc gaagccaatg acgttctctt catcgacgag attcaccggc tgtcgcccgt cgtcgaggaa atcctgtatc cggcgctcga ggattatcag atcgatatca tgatcggcga aggccccgcg gcgcgcagtg tgaagctcga tctgcaaccg ttcacgctgg tcggcgccac gacgcgcgcg ggcatgctga ccaatccgtt gcgcgaccgc tttggtattg tcgcgcgatt ggagttttat aacgcagaag agctggcacg catcgtgagc cgttccgcat cgctgctcgg cgcgcagatc cacccggaag gtgcactgga aatcgcgaag cgctcgcgcg gcacgccgcg tatcgcgaac cgtctgctgc ggcgcgtgcg cgattacgcg gaagtgaagg cggacggcaa tatcaccgcg aaggtcgcgg atgcagcgct gaaaatgctc gacgtcgatc cggtcggttt tgatctgatg gaccgcaagc ttctcgaagc gatcttgcac aagttcgatg gcggtcccgt gggcgtcgac aatctcgcgg cggcaattgg cgaagagcgc gacacaatcg aagacgtgct cgagccgtat ctgatccagc agggctttct gcaacgcacg ccgcgtggac gcgtcgccac gttgttgacg tatcggcact tcgggcttgc cgcacctgac tcggcaagtc ctgttcgcaa tctgtgggat gccgagccga gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuphA.00013.a.B1.PS38154
PURIFICATION DATe: 12/16/2016
CONCENTRATION: 30.77mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG HARYNARMIE HDKLAAERII AATPVSPNEE AFERALRPRQ LDEYVGQEKV RGQLEIFIEA AKRRSESLDH VLLFGPPGLG KTTLAHIIAR EMGVNLRQTS GPVLERAGDL AALLTNLEAN DVLFIDEIHR LSPVVEEILY PALEDYQIDI MIGEGPAARS VKLDLQPFTL VGATTRAGML TNPLRDRFGI VARLEFYNAE ELARIVSRSA SLLGAQIHPE GALEIAKRSR GTPRIANRLL RRVRDYAEVK ADGNITAKVA DAALKMLDVD PVGFDLMDRK LLEAILHKFD GGPVGVDNLA AAIGEERDTI EDVLEPYLIQ QGFLQRTPRG RVATLLTYRH FGLAAPDSAS PVX
Validated NT Sequence
ttnngaacag gacttgccga gtcaggtgcg gcaagcccga agtgccgata cgtcaacaac gtggcgacgc gtccacgcgg cgtgcgttgc agaaagccct gctggatcag atacggctcg agcacgtctt cgattgtgtc gcgctcttcg ccaattgccg ccgcgagatt gtcgacgccc acgggaccgc catcgaactt gtgcaagatc gcttcgagaa gcttgcggtc catcagatca aaaccgaccg gatcgacgtc gagcattttc agcgctgcat ccgcgacctt cgcggtgata ttgccgtccg ccttcacttc cgcgtaatcg cgcacgcgcc gcagcagacg gttcgcgata cgcggcgtgc cgcgcgagcg cttcgcgatt tccagtgcac cttccgggtg gatctgcgcg ccgagcagcg atgcggaacg gctcacgatg cgtgccagct cttctgcgtt ataaaactcc aatcgcgcga caataccaaa gcggtcgcgc aacggattgg tcagcatgcc cgcgcgcgtc gtggcgccga ccagcgtgaa cggttgcaga tcgagcttca cactgcgcgc cgcggggcct tcgccgatca tgatatcgat ctgataatcc tcgagcgccg gatacaggat ttcctcgacg acgggcgaca gccggtgaat ctcgtcgatg aagagaacgt cattggcttc gaggttggtc agaagcgccg cgagatcgcc cgcgcgctcc agcacgggac cggaagtctg ccgcagattc acgcccattt cgcgcgcgat gatatgcgcg agcgtggtct tgccgaggcc aggcggaccg aacagcagca cgtgatccag cgattccgag cggcgcttgg cagcctcgat gaaaatttcc agctggccgc gcaccttttc ctggccgacg tattcgtcca gctgacgggg gcgcaacgcg cgctcgaacg cttcctcgtt cggcgacacg ggcgtggcgg cgatgatgcg ttcggcggcg agtttgtcgt gttcgatcat gcgggcattg taccgcgcgt ggcccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMG HARYNARMIE HDKLAAERII AATPVSPNEE AFERALRPRQ LDEYVGQEKV RGQLEIFIEA AKRRSESLDH VLLFGPPGLG KTTLAHIIAR EMGVNLRQTS GPVLERAGDL AALLTNLEAN DVLFIDEIHR LSPVVEEILY PALEDYQIDI MIGEGPAARS VKLDLQPFTL VGATTRAGML TNPLRDRFGI VARLEFYNAE ELARIVSRSA SLLGAQIHPE GALEIAKRSR GTPRIANRLL RRVRDYAEVK ADGNITAKVA DAALKMLDVD PVGFDLMDRK LLEAILHKFD GGPVGVDNLA AAIGEERDTI EDVLEPYLIQ QGFLQRTPRG RVATLLTYRH FGLAAPDSAS PVRNLWDAEP S
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGCC ACGCGCGGTA CAATGCCCGC ATGATCGAAC ACGACAAACT CGCCGCCGAA CGCATCATCG CCGCCACGCC CGTGTCGCCG AACGAGGAAG CGTTCGAGCG CGCGTTGCGC CCCCGTCAGC TGGACGAATA CGTCGGCCAG GAAAAGGTGC GCGGCCAGCT GGAAATTTTC ATCGAGGCTG CCAAGCGCCG CTCGGAATCG CTGGATCACG TGCTGCTGTT CGGTCCGCCT GGCCTCGGCA AGACCACGCT CGCGCATATC ATCGCGCGCG AAATGGGCGT GAATCTGCGG CAGACTTCCG GTCCCGTGCT GGAGCGCGCG GGCGATCTCG CGGCGCTTCT GACCAACCTC GAAGCCAATG ACGTTCTCTT CATCGACGAG ATTCACCGGC TGTCGCCCGT CGTCGAGGAA ATCCTGTATC CGGCGCTCGA GGATTATCAG ATCGATATCA TGATCGGCGA AGGCCCCGCG GCGCGCAGTG TGAAGCTCGA TCTGCAACCG TTCACGCTGG TCGGCGCCAC GACGCGCGCG GGCATGCTGA CCAATCCGTT GCGCGACCGC TTTGGTATTG TCGCGCGATT GGAGTTTTAT AACGCAGAAG AGCTGGCACG CATCGTGAGC CGTTCCGCAT CGCTGCTCGG CGCGCAGATC CACCCGGAAG GTGCACTGGA AATCGCGAAG CGCTCGCGCG GCACGCCGCG TATCGCGAAC CGTCTGCTGC GGCGCGTGCG CGATTACGCG GAAGTGAAGG CGGACGGCAA TATCACCGCG AAGGTCGCGG ATGCAGCGCT GAAAATGCTC GACGTCGATC CGGTCGGTTT TGATCTGATG GACCGCAAGC TTCTCGAAGC GATCTTGCAC AAGTTCGATG GCGGTCCCGT GGGCGTCGAC AATCTCGCGG CGGCAATTGG CGAAGAGCGC GACACAATCG AAGACGTGCT CGAGCCGTAT CTGATCCAGC AGGGCTTTCT GCAACGCACG CCGCGTGGAC GCGTCGCCAC GTTGTTGACG TATCGGCACT TCGGGCTTGC CGCACCTGAC TCGGCAAGTC CTGTTCGCAA TCTGTGGGAT GCCGAGCCGA GCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT