BuphA.00010.t

Short-chain dehydrogenase/reductase SDR

CENTER ID: BuphA.00010.t
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00010.t.B2.GE39385 Mature protein( BuphA.00010.t ) 21 256
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.200
RefSeq: YP_001856424.1
UniProt: B2JKI9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSPLKVFIT GASSGIGEAL AAEYARRGAI LGLVARRGDA LARFRASHPQ NTISVYAVDV RDADALAHAA QRFIAEHGCP DIVIANAGIS RGAVTGHGDL QTFRDVMDIN YFGMVATFEP FAQPMIAART GALVGIASVA GVRGLPGSGA YSASKSAALK YLEALRVEMR PLGVSVTTIA PGYIRTPMTA HNPYSMPFLM DADRFAAKVA DAVARRKRFA IFPWQMRFAA MLLHVAPRWL YDAVFEKAPR KPRAAQ
NT Sequence
ATGAGCTCAC CGCTGAAGGT CTTCATCACC GGCGCGTCCA GCGGCATCGG CGAAGCGCTC GCCGCCGAAT ACGCGCGGCG CGGCGCAATC CTCGGCCTCG TTGCCCGCCG CGGCGACGCA CTCGCCCGCT TCCGGGCGTC GCATCCCCAG AACACCATCT CCGTGTATGC CGTCGACGTA CGGGACGCGG ACGCGCTCGC ACATGCGGCG CAGCGCTTCA TCGCCGAGCA CGGCTGCCCC GACATCGTGA TCGCCAACGC GGGCATCAGC CGGGGCGCCG TCACGGGTCA CGGCGATCTG CAGACGTTTC GCGACGTGAT GGACATCAAC TACTTCGGCA TGGTCGCGAC CTTCGAGCCG TTCGCGCAAC CGATGATCGC GGCGCGCACG GGCGCGCTCG TCGGCATTGC GAGCGTCGCG GGCGTCCGCG GCTTGCCTGG TTCGGGCGCG TACAGCGCAT CGAAATCGGC GGCGCTCAAG TATCTGGAAG CCCTGCGTGT CGAGATGCGG CCGCTTGGGG TGTCGGTAAC GACGATTGCG CCCGGCTATA TCCGCACGCC AATGACGGCG CACAACCCGT ACTCGATGCC GTTCCTCATG GACGCGGACC GTTTCGCCGC CAAAGTCGCC GATGCGGTCG CGCGCAGGAA GCGCTTTGCA ATCTTCCCGT GGCAGATGCG CTTTGCGGCA ATGCTGCTGC ACGTCGCGCC GCGCTGGCTC TACGACGCGG TGTTCGAAAA GGCGCCCCGC AAGCCGCGCG CGGCGCAG
Details for BuphA.00010.t.B2.GE39385
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 27kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA AEYARRGAIL GLVARRGDAL ARFRASHPQN TISVYAVDVR DADALAHAAQ RFIAEHGCPD IVIANAGISR GAVTGHGDLQ TFRDVMDINY FGMVATFEPF AQPMIAARTG ALVGIASVAG VRGLPGSGAY SASKSAALKY LEALRVEMRP LGVSVTTIAP GYIRTPMTAH NPYSMPFLMD ADRFAAKVAD AVARRKRFAI FPWQMRFAAM LLHVAPRWLY DAVFEKAPRK PRAAQ
Validated NT Sequence
atcttactca cttactgcgc cgcgcgcggc ttgcggggcg ccttttcgaa caccgcgtcg tagagccagc gcggcgcgac gtgcagcagc attgccgcaa agcgcatctg ccacgggaag attgcaaagc gcttcctgcg cgcgaccgca tcggcgactt tggcggcgaa acggtccgcg tccatgagga acggcatcga gtacgggttg tgcgccgtca ttggcgtgcg gatatagccg ggcgcaatcg tcgttaccga caccccaagc ggccgcatct cgacacgcag ggcttccaga tacttgagcg ccgccgattt cgatgcgctg tacgcgcccg aaccaggcaa gccgcggacg cccgcgacgc tcgcaatgcc gacgagcgcg cccgtgcgcg ccgcgatcat cggttgcgcg aacggctcga aggtcgcgac catgccgaag tagttgatgt ccatcacgtc gcgaaacgtc tgcagatcgc cgtgacccgt gacggcgccc cggctgatgc ccgcgttggc gatcacgatg tcggggcagc cgtgctcggc gatgaagcgc tgcgccgcat gtgcgagcgc gtccgcgtcc cgtacgtcga cggcatacac ggagatggtg ttctggggat gcgacgcccg gaagcgggcg agtgcgtcgc cgcggcgggc aacgaggccg aggattgcgc cgcgccgcgc gtattcggcg gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHAA EYARRGAILG LVARRGDALA RFRASHPQNT ISVYAVDVRD ADALAHAAQR FIAEHGCPDI VIANAGISRG AVTGHGDLQT FRDVMDINYF GMVATFEPFA QPMIAARTGA LVGIASVAGV RGLPGSGAYS ASKSAALKYL EALRVEMRPL GVSVTTIAPG YIRTPMTAHN PYSMPFLMDA DRFAAKVADA VARRKRFAIF PWQMRFAAML LHVAPRWLYD AVFEKAPRKP RAAQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgccg aatacgcgcg gcgcggcgca atcctcggcc tcgttgcccg ccgcggcgac gcactcgccc gcttccgggc gtcgcatccc cagaacacca tctccgtgta tgccgtcgac gtacgggacg cggacgcgct cgcacatgcg gcgcagcgct tcatcgccga gcacggctgc cccgacatcg tgatcgccaa cgcgggcatc agccggggcg ccgtcacggg tcacggcgat ctgcagacgt ttcgcgacgt gatggacatc aactacttcg gcatggtcgc gaccttcgag ccgttcgcgc aaccgatgat cgcggcgcgc acgggcgcgc tcgtcggcat tgcgagcgtc gcgggcgtcc gcggcttgcc tggttcgggc gcgtacagcg catcgaaatc ggcggcgctc aagtatctgg aagccctgcg tgtcgagatg cggccgcttg gggtgtcggt aacgacgatt gcgcccggct atatccgcac gccaatgacg gcgcacaacc cgtactcgat gccgttcctc atggacgcgg accgtttcgc cgccaaagtc gccgatgcgg tcgcgcgcag gaagcgcttt gcaatcttcc cgtggcagat gcgctttgcg gcaatgctgc tgcacgtcgc gccgcgctgg ctctacgacg cggtgttcga aaaggcgccc cgcaagccgc gcgcggcgca gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat