SSGCID: Target Page

BuphA.00010.r

Short-chain dehydrogenase/reductase SDR

CENTER ID:	BuphA.00010.r
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00010.r.B1.GE39383	Full length( BuphA.00010.r )		1	276

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.3070
RefSeq:	YP_001859112.1
UniProt:	B2JIM0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTVSNDTFAA RAQPHRPVRT VLVTGAAHRI GRGLAVGFAA AGWDVAVHYG SSQEQADGVV AEIAALGRRA VALKADLSVE AEVERLVPDC MAALGRPSCI VNNASLFDED TAFDVGYAKL LHLTSINLGA PLVLARMLHE VTPDDARHDE SLRTCVINVL DQKLYNMNPD YLSYTLSKAA LGTATVALAQ ALAPKVRVVG LAPGLTLQSG DQTPASFADA HKVTPLGRAS RVEDLVAAAL YLADARGVTG TTLVVDGGQH LVPLPRDVMF LTGGDG

NT Sequence

ATGACCGTCT CGAACGATAC CTTCGCCGCC CGCGCCCAGC CGCACCGGCC CGTCCGCACC GTGCTCGTCA CGGGCGCCGC GCATCGGATC GGCAGAGGGC TGGCCGTCGG GTTCGCCGCG GCGGGCTGGG ACGTGGCGGT GCATTACGGG TCGTCGCAGG AACAGGCCGA CGGCGTAGTC GCCGAAATCG CCGCGCTGGG ACGGCGGGCG GTCGCGCTGA AGGCGGATCT GTCCGTCGAG GCGGAAGTCG AGCGGCTCGT GCCCGATTGC ATGGCGGCGC TCGGGCGTCC GTCCTGCATC GTGAACAACG CGTCGCTGTT CGACGAGGAC ACCGCGTTCG ATGTCGGTTA CGCGAAGCTG CTGCACCTGA CGTCGATCAA CCTCGGCGCG CCGCTCGTGC TTGCGCGGAT GCTGCACGAA GTCACGCCGG ACGACGCGCG GCACGATGAA TCGCTGCGCA CGTGCGTGAT CAACGTGCTC GACCAGAAGC TGTACAACAT GAATCCGGAT TACCTGTCGT ACACACTGTC GAAGGCCGCG CTCGGCACGG CGACCGTCGC GCTCGCGCAG GCGCTGGCGC CGAAGGTGCG TGTCGTGGGT CTCGCGCCAG GCCTCACGCT GCAATCGGGC GATCAGACGC CAGCCAGCTT CGCGGATGCG CACAAGGTGA CGCCGCTCGG CCGGGCGTCG CGCGTCGAGG ATCTCGTCGC GGCGGCGCTG TATCTCGCGG ATGCGCGCGG CGTGACGGGC ACGACGCTTG TCGTCGATGG CGGCCAGCAT CTCGTGCCGC TGCCGCGCGA CGTGATGTTC TTGACGGGCG GCGACGGC

Details for BuphA.00010.r.B1.GE39383

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT VSNDTFAARA QPHRPVRTVL VTGAAHRIGR GLAVGFAAAG WDVAVHYGSS QEQADGVVAE IAALGRRAVA LKADLSVEAE VERLVPDCMA ALGRPSCIVN NASLFDEDTA FDVGYAKLLH LTSINLGAPL VLARMLHEVT PDDARHDESL RTCVINVLDQ KLYNMNPDYL SYTLSKAALG TATVALAQAL APKVRVVGLA PGLTLQSGDQ TPASFADAHK VTPLGRASRV EDLVAAALYL ADARGVTGTT LVVDGGQHLV PLPRDVXXXT GGDG

Validated NT Sequence

ttactcactt agccgtcgcc gcccgtcann nannnnacgt cgcgcggcag cggcacgaga tgctggccgc catcgacgac aagcgtcgtg cccgtcacgc cgcgcgcatc cgcgagatac agcgccgccg cgacgagatc ctcgacgcgc gacgcccggc cgagcggcgt caccttgtgc gcatccgcga agctggctgg cgtctgatcg cccgattgca gcgtgaggcc tggcgcgaga cccacgacac gcaccttcgg cgccagcgcc tgcgcgagcg cgacggtcgc cgtgccgagc gcggccttcg acagtgtgta cgacaggtaa tccggattca tgttgtacag cttctggtcg agcacgttga tcacgcacgt gcgcagcgat tcatcgtgcc gcgcgtcgtc cggcgtgact tcgtgcagca tccgcgcaag cacgagcggc gcgccgaggt tgatcgacgt caggtgcagc agcttcgcgt aaccgacatc gaacgcggtg tcctcgtcga acagcgacgc gttgttcacg atgcaggacg gacgcccgag cgccgccatg caatcgggca cgagccgctc gacttccgcc tcgacggaca gatccgcctt cagcgcgacc gcccgccgtc ccagcgcggc gatttcggcg actacgccgt cggcctgttc ctgcgacgac ccgtaatgca ccgccacgtc ccagcccgcc gcggcgaacc cgacggccag ccctctgccg atccgatgcg cggcgcccgt gacgagcacg gtgcggacgg gccggtgcgg ctgggcgcgg gcggcgaagg tatcgttcga gacggtcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg tctcgaacga taccttcgcc gcccgcgccc agccgcaccg gcccgtccgc accgtgctcg tcacgggcgc cgcgcatcgg atcggcagag ggctggccgt cgggttcgcc gcggcgggct gggacgtggc ggtgcattac gggtcgtcgc aggaacaggc cgacggcgta gtcgccgaaa tcgccgcgct gggacggcgg gcggtcgcgc tgaaggcgga tctgtccgtc gaggcggaag tcgagcggct cgtgcccgat tgcatggcgg cgctcgggcg tccgtcctgc atcgtgaaca acgcgtcgct gttcgacgag gacaccgcgt tcgatgtcgg ttacgcgaag ctgctgcacc tgacgtcgat caacctcggc gcgccgctcg tgcttgcgcg gatgctgcac gaagtcacgc cggacgacgc gcggcacgat gaatcgctgc gcacgtgcgt gatcaacgtg ctcgaccaga agctgtacaa catgaatccg gattacctgt cgtacacact gtcgaaggcc gcgctcggca cggcgaccgt cgcgctcgcg caggcgctgg cgccgaaggt gcgtgtcgtg ggtctcgcgc caggcctcac gctgcaatcg ggcgatcaga cgccagccag cttcgcggat gcgcacaagg tgacgccgct cggccgggcg tcgcgcgtcg aggatctcgt cgcggcggcg ctgtatctcg cggatgcgcg cggcgtgacg ggcacgacgc ttgtcgtcga tggcggccag catctcgtgc cgctgccgcg cgacgtgatg ttcttgacgg gcggcgacgg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat