BuphA.00010.p

Short-chain dehydrogenase/reductase SDR

CENTER ID: BuphA.00010.p
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00010.p.B2.GE39382 Mature protein( BuphA.00010.p ) 27 258
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.2776
RefSeq: YP_001858834.1
UniProt: B2JH04

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRLQGKVAL VTGAGRGIGA AIATAFAREG AAVVLAELDV ETARRTAAEI AAQTGGKTLA VQTDVTQSAS VQRAVSEGET AFGAIDVLVN NAGINVFCDP LTMTDDDWRR CFAVDLDGVW NGCRAVLPGM VERGAGSIVN IASTHAFKII PGCFPYPVAK HGVIGLTRAL GIEYAPRNVR VNAIAPGYIE TQLTRDWWDG QADPAAAKQA TLDLQPMGRI GKPEEVAMTA VFLASDEAPF INASCITVDG GRSALYHD
NT Sequence
ATGAAACGGC TGCAAGGGAA GGTTGCGCTG GTGACGGGCG CCGGACGGGG CATCGGCGCG GCGATCGCGA CGGCGTTCGC GCGCGAGGGC GCGGCTGTCG TGCTCGCGGA ACTCGACGTC GAAACGGCGC GGCGCACCGC CGCTGAAATC GCCGCGCAGA CGGGCGGCAA GACGCTCGCC GTGCAGACGG ACGTCACGCA ATCGGCCTCC GTGCAGCGCG CCGTAAGCGA AGGCGAAACT GCGTTCGGCG CGATCGACGT GCTGGTGAAC AACGCGGGCA TCAACGTGTT CTGCGATCCG CTGACGATGA CCGACGACGA CTGGCGCCGC TGTTTCGCCG TCGATCTCGA CGGTGTGTGG AATGGTTGCC GCGCGGTGCT GCCGGGAATG GTGGAACGTG GCGCGGGCAG CATCGTGAAC ATCGCGTCGA CACACGCGTT CAAGATCATT CCGGGCTGCT TCCCATACCC GGTCGCCAAG CACGGTGTGA TCGGCCTCAC GCGCGCGCTC GGTATCGAAT ACGCGCCGCG CAACGTACGG GTGAACGCGA TCGCGCCGGG CTACATCGAA ACGCAACTGA CGCGCGACTG GTGGGACGGC CAGGCCGATC CCGCCGCCGC GAAGCAGGCG ACGCTCGACC TGCAGCCGAT GGGACGTATC GGCAAGCCGG AAGAAGTGGC GATGACGGCC GTCTTTCTCG CGTCGGATGA AGCGCCGTTC ATCAACGCGA GCTGTATCAC CGTCGACGGC GGGCGTTCGG CGCTCTATCA CGAC
Details for BuphA.00010.p.B2.GE39382
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA REGAAVVLAE LDVETARRTA AEIAAQTGGK TLAVQTDVTQ SASVQRAVSE GETAFGAIDV LVNNAGINVF CDPLTMTDDD WRRCFAVDLD GVWNGCRAVL PGMVERGAGS IVNIASTHAF KIIPGCFPYP VAKHGVIGLT RALGIEYAPR NVRVNAIAPG YIETQLTRDW WDGQADPAAA KQATLDLQPM GRIGKPEEVA MTAVFLASDE APFINASCIT VDGGRSALYH D
Validated NT Sequence
atcttactca cttagtcgtg atagagcgcc gaacgcccgc cgtcgacggt gatacagctc gcgttgatga acggcgcttc atccgacgcg agaaagacgg ccgtcatcgc cacttcttcc ggcttgccga tacgtcccat cggctgcagg tcgagcgtcg cctgcttcgc ggcggcggga tcggcctggc cgtcccacca gtcgcgcgtc agttgcgttt cgatgtagcc cggcgcgatc gcgttcaccc gtacgttgcg cggcgcgtat tcgataccga gcgcgcgcgt gaggccgatc acaccgtgct tggcgaccgg gtatgggaag cagcccggaa tgatcttgaa cgcgtgtgtc gacgcgatgt tcacgatgct gcccgcgcca cgttccacca ttcccggcag caccgcgcgg caaccattcc acacaccgtc gagatcgacg gcgaaacagc ggcgccagtc gtcgtcggtc atcgtcagcg gatcgcagaa cacgttgatg cccgcgttgt tcaccagcac gtcgatcgcg ccgaacgcag tttcgccttc gcttacggcg cgctgcacgg aggccgattg cgtgacgtcc gtctgcacgg cgagcgtctt gccgcccgtc tgcgcggcga tttcagcggc ggtgcgccgc gccgtttcga cgtcgagttc cgcgagcacg acagccgcgc cctcgcgcgc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHAR EGAAVVLAEL DVETARRTAA EIAAQTGGKT LAVQTDVTQS ASVQRAVSEG ETAFGAIDVL VNNAGINVFC DPLTMTDDDW RRCFAVDLDG VWNGCRAVLP GMVERGAGSI VNIASTHAFK IIPGCFPYPV AKHGVIGLTR ALGIEYAPRN VRVNAIAPGY IETQLTRDWW DGQADPAAAK QATLDLQPMG RIGKPEEVAM TAVFLASDEA PFINASCITV DGGRSALYHD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgcgcg agggcgcggc tgtcgtgctc gcggaactcg acgtcgaaac ggcgcggcgc accgccgctg aaatcgccgc gcagacgggc ggcaagacgc tcgccgtgca gacggacgtc acgcaatcgg cctccgtgca gcgcgccgta agcgaaggcg aaactgcgtt cggcgcgatc gacgtgctgg tgaacaacgc gggcatcaac gtgttctgcg atccgctgac gatgaccgac gacgactggc gccgctgttt cgccgtcgat ctcgacggtg tgtggaatgg ttgccgcgcg gtgctgccgg gaatggtgga acgtggcgcg ggcagcatcg tgaacatcgc gtcgacacac gcgttcaaga tcattccggg ctgcttccca tacccggtcg ccaagcacgg tgtgatcggc ctcacgcgcg cgctcggtat cgaatacgcg ccgcgcaacg tacgggtgaa cgcgatcgcg ccgggctaca tcgaaacgca actgacgcgc gactggtggg acggccaggc cgatcccgcc gccgcgaagc aggcgacgct cgacctgcag ccgatgggac gtatcggcaa gccggaagaa gtggcgatga cggccgtctt tctcgcgtcg gatgaagcgc cgttcatcaa cgcgagctgt atcaccgtcg acggcgggcg ttcggcgctc tatcacgact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat