BuphA.00010.n
Short-chain dehydrogenase/reductase SDR
| CENTER ID: | BuphA.00010.n |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00010.n.B1.GE39381 | Full length( BuphA.00010.n ) | 1 | 289 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00010.n.B1.PS02549 | Full length( BuphA.00010.n ) | 1 | 289 | ||
| BuphA.00010.n.B1.PS37820 | Full length( BuphA.00010.n ) | 1 | 289 |
Structures
5IG2
DEPOSITED: 2/26/2016
DETERMINATION: XRay
CLONE: BuphA.00010.n.B1.GE39381
PROTEIN: BuphA.00010.n.B1.PS02549
Publications by SSGCID
Crystal structure of a short chain dehydrogenase/reductase from Burkholderia phymatum in complex with NAD
Myler PJ, Subramanian S, Mayclin SJ, Asojo OA, Alenazi J
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2022
volume 78, issue Pt 2, pages 52-58
PMID: 35102893; PMCID: PMC8805215
PDB:
5IG2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_139105 |
| PATRIC ID: | fig|391038.7.peg.1097 |
| RefSeq: | YP_001857273.1 |
| UniProt: | B2JGP2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFEFDGKVAV ITGAGSGFGR AFAEKGASLG MKLVLADVDE GALARTVDTL RAAGAEVIGV RTDVSNGAQV QALADAALEA FGKVHLLFNN AGVGAGGFLW ESSANDWAWV FGVNVMGVAH GVRVFAPIML GQNEAAHIVN TASVAGLLSP PSMGIYNASK HAVVSLTETL YHDLRNAGGE VGCSLLCPAF VPTGIADAER VRPEALRNEA QPTRSQLAAD RQLQRAVRSG KLGATDVATL TFEAIAERRF YILTHPAILA TVRLRHEDIE LQRNPTDPLS LKPEVKEAR
NT Sequence
ATGTTCGAAT TCGACGGCAA GGTCGCGGTG ATAACAGGCG CGGGAAGCGG CTTTGGCCGT GCGTTTGCGG AGAAGGGCGC GTCGCTCGGC ATGAAGCTCG TGCTGGCGGA TGTCGACGAA GGCGCGCTCG CACGAACTGT CGATACGTTG CGCGCAGCGG GCGCCGAGGT GATCGGCGTG CGCACGGACG TGTCGAATGG CGCGCAGGTT CAGGCGCTCG CCGATGCGGC GCTCGAAGCG TTCGGCAAGG TGCATCTGCT GTTCAACAAC GCGGGCGTCG GCGCCGGCGG CTTTCTATGG GAAAGCTCGG CGAACGACTG GGCGTGGGTG TTCGGCGTCA ACGTGATGGG CGTCGCGCAC GGCGTGCGCG TCTTCGCGCC CATCATGCTC GGGCAGAACG AAGCGGCGCA TATCGTCAAT ACGGCGTCGG TGGCGGGCCT GCTGTCGCCG CCGTCGATGG GCATCTACAA CGCGTCGAAG CATGCGGTGG TTTCGCTGAC CGAGACGCTG TATCACGACC TGCGCAATGC GGGCGGCGAG GTGGGCTGTT CGCTGCTGTG TCCCGCGTTC GTGCCCACAG GTATCGCCGA CGCCGAACGC GTGCGCCCCG AGGCGTTGCG CAACGAAGCG CAACCCACGC GCTCGCAGCT CGCCGCCGAC AGGCAACTGC AGCGTGCAGT GCGGTCCGGC AAGTTGGGCG CGACCGACGT CGCGACGCTC ACCTTCGAAG CGATCGCGGA ACGCCGCTTC TACATCCTCA CGCATCCCGC CATTCTCGCG ACGGTGCGGC TTCGTCACGA GGACATCGAA CTGCAGCGCA ATCCGACCGA TCCGTTGTCG CTGAAGCCCG AAGTCAAGGA AGCCCGC
Details for BuphA.00010.n.B1.GE39381
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMF EFDGKVAVIT GAGSGFGRAF AEKGASLGMK LVLADVDEGA LARTVDTLRA AGAEVIGVRT DVSNGAQVQA LADAALEAFG KVHLLFNNAG VGAGGFLWES SANDWAWVFG VNVMGVAHGV RVFAPIMLGQ NEAAHIVNTA SVAGLLSPPS MGIYNASKHA VVSLTETLYH DLRNAGGEVG CSLLCPAFVP TGIADAERVR PEALRNEAQP TRSQLAADRQ LQRAVRSGKL GATDVATLTF EAIAERRFYI LTHPAILATV RLRHEDIELQ RNPTDPLSLX XXVKEAR
Validated NT Sequence
ttactcactt agcgggcttc cttgacttnn gnnntcagcg acaacggatc ggtcggattg cgctgcagtt cgatgtcctc gtgacgaagc cgcaccgtcg cgagaatggc gggatgcgtg aggatgtaga agcggcgttc cgcgatcgct tcgaaggtga gcgtcgcgac gtcggtcgcg cccaacttgc cggaccgcac tgcacgctgc agttgcctgt cggcggcgag ctgcgagcgc gtgggttgcg cttcgttgcg caacgcctcg gggcgcacgc gttcggcgtc ggcgatacct gtgggcacga acgcgggaca cagcagcgaa cagcccacct cgccgcccgc attgcgcagg tcgtgataca gcgtctcggt cagcgaaacc accgcatgct tcgacgcgtt gtagatgccc atcgacggcg gcgacagcag gcccgccacc gacgccgtat tgacgatatg cgccgcttcg ttctgcccga gcatgatggg cgcgaagacg cgcacgccgt gcgcgacgcc catcacgttg acgccgaaca cccacgccca gtcgttcgcc gagctttccc atagaaagcc gccggcgccg acgcccgcgt tgttgaacag cagatgcacc ttgccgaacg cttcgagcgc cgcatcggcg agcgcctgaa cctgcgcgcc attcgacacg tccgtgcgca cgccgatcac ctcggcgccc gctgcgcgca acgtatcgac agttcgtgcg agcgcgcctt cgtcgacatc cgccagcacg agcttcatgc cgagcgacgc gcccttctcc gcaaacgcac ggccaaagcc gcttcccgcg cctgttatca ccgcgacctt gccgtcgaat tcgaacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMF EFDGKVAVIT GAGSGFGRAF AEKGASLGMK LVLADVDEGA LARTVDTLRA AGAEVIGVRT DVSNGAQVQA LADAALEAFG KVHLLFNNAG VGAGGFLWES SANDWAWVFG VNVMGVAHGV RVFAPIMLGQ NEAAHIVNTA SVAGLLSPPS MGIYNASKHA VVSLTETLYH DLRNAGGEVG CSLLCPAFVP TGIADAERVR PEALRNEAQP TRSQLAADRQ LQRAVRSGKL GATDVATLTF EAIAERRFYI LTHPAILATV RLRHEDIELQ RNPTDPLSLK PEVKEAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcg aattcgacgg caaggtcgcg gtgataacag gcgcgggaag cggctttggc cgtgcgtttg cggagaaggg cgcgtcgctc ggcatgaagc tcgtgctggc ggatgtcgac gaaggcgcgc tcgcacgaac tgtcgatacg ttgcgcgcag cgggcgccga ggtgatcggc gtgcgcacgg acgtgtcgaa tggcgcgcag gttcaggcgc tcgccgatgc ggcgctcgaa gcgttcggca aggtgcatct gctgttcaac aacgcgggcg tcggcgccgg cggctttcta tgggaaagct cggcgaacga ctgggcgtgg gtgttcggcg tcaacgtgat gggcgtcgcg cacggcgtgc gcgtcttcgc gcccatcatg ctcgggcaga acgaagcggc gcatatcgtc aatacggcgt cggtggcggg cctgctgtcg ccgccgtcga tgggcatcta caacgcgtcg aagcatgcgg tggtttcgct gaccgagacg ctgtatcacg acctgcgcaa tgcgggcggc gaggtgggct gttcgctgct gtgtcccgcg ttcgtgccca caggtatcgc cgacgccgaa cgcgtgcgcc ccgaggcgtt gcgcaacgaa gcgcaaccca cgcgctcgca gctcgccgcc gacaggcaac tgcagcgtgc agtgcggtcc ggcaagttgg gcgcgaccga cgtcgcgacg ctcaccttcg aagcgatcgc ggaacgccgc ttctacatcc tcacgcatcc cgccattctc gcgacggtgc ggcttcgtca cgaggacatc gaactgcagc gcaatccgac cgatccgttg tcgctgaagc ccgaagtcaa ggaagcccgc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuphA.00010.n.B1.PS02549
| PURIFICATION DATe: | 12/8/2015 |
| CONCENTRATION: | 45mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF EFDGKVAVIT GAGSGFGRAF AEKGASLGMK LVLADVDEGA LARTVDTLRA AGAEVIGVRT DVSNGAQVQA LADAALEAFG KVHLLFNNAG VGAGGFLWES SANDWAWVFG VNVMGVAHGV RVFAPIMLGQ NEAAHIVNTA SVAGLLSPPS MGIYNASKHA VVSLTETLYH DLRNAGGEVG CSLLCPAFVP TGIADAERVR PEALRNEAQP TRSQLAADRQ LQRAVRSGKL GATDVATLTF EAIAERRFYI LTHPAILATV RLRHEDIELQ RNPTDPLSLX XXVKEAR
Validated NT Sequence
ttactcactt agcgggcttc cttgacttnn gnnntcagcg acaacggatc ggtcggattg cgctgcagtt cgatgtcctc gtgacgaagc cgcaccgtcg cgagaatggc gggatgcgtg aggatgtaga agcggcgttc cgcgatcgct tcgaaggtga gcgtcgcgac gtcggtcgcg cccaacttgc cggaccgcac tgcacgctgc agttgcctgt cggcggcgag ctgcgagcgc gtgggttgcg cttcgttgcg caacgcctcg gggcgcacgc gttcggcgtc ggcgatacct gtgggcacga acgcgggaca cagcagcgaa cagcccacct cgccgcccgc attgcgcagg tcgtgataca gcgtctcggt cagcgaaacc accgcatgct tcgacgcgtt gtagatgccc atcgacggcg gcgacagcag gcccgccacc gacgccgtat tgacgatatg cgccgcttcg ttctgcccga gcatgatggg cgcgaagacg cgcacgccgt gcgcgacgcc catcacgttg acgccgaaca cccacgccca gtcgttcgcc gagctttccc atagaaagcc gccggcgccg acgcccgcgt tgttgaacag cagatgcacc ttgccgaacg cttcgagcgc cgcatcggcg agcgcctgaa cctgcgcgcc attcgacacg tccgtgcgca cgccgatcac ctcggcgccc gctgcgcgca acgtatcgac agttcgtgcg agcgcgcctt cgtcgacatc cgccagcacg agcttcatgc cgagcgacgc gcccttctcc gcaaacgcac ggccaaagcc gcttcccgcg cctgttatca ccgcgacctt gccgtcgaat tcgaacatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMF EFDGKVAVIT GAGSGFGRAF AEKGASLGMK LVLADVDEGA LARTVDTLRA AGAEVIGVRT DVSNGAQVQA LADAALEAFG KVHLLFNNAG VGAGGFLWES SANDWAWVFG VNVMGVAHGV RVFAPIMLGQ NEAAHIVNTA SVAGLLSPPS MGIYNASKHA VVSLTETLYH DLRNAGGEVG CSLLCPAFVP TGIADAERVR PEALRNEAQP TRSQLAADRQ LQRAVRSGKL GATDVATLTF EAIAERRFYI LTHPAILATV RLRHEDIELQ RNPTDPLSLK PEVKEAR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTCG AATTCGACGG CAAGGTCGCG GTGATAACAG GCGCGGGAAG CGGCTTTGGC CGTGCGTTTG CGGAGAAGGG CGCGTCGCTC GGCATGAAGC TCGTGCTGGC GGATGTCGAC GAAGGCGCGC TCGCACGAAC TGTCGATACG TTGCGCGCAG CGGGCGCCGA GGTGATCGGC GTGCGCACGG ACGTGTCGAA TGGCGCGCAG GTTCAGGCGC TCGCCGATGC GGCGCTCGAA GCGTTCGGCA AGGTGCATCT GCTGTTCAAC AACGCGGGCG TCGGCGCCGG CGGCTTTCTA TGGGAAAGCT CGGCGAACGA CTGGGCGTGG GTGTTCGGCG TCAACGTGAT GGGCGTCGCG CACGGCGTGC GCGTCTTCGC GCCCATCATG CTCGGGCAGA ACGAAGCGGC GCATATCGTC AATACGGCGT CGGTGGCGGG CCTGCTGTCG CCGCCGTCGA TGGGCATCTA CAACGCGTCG AAGCATGCGG TGGTTTCGCT GACCGAGACG CTGTATCACG ACCTGCGCAA TGCGGGCGGC GAGGTGGGCT GTTCGCTGCT GTGTCCCGCG TTCGTGCCCA CAGGTATCGC CGACGCCGAA CGCGTGCGCC CCGAGGCGTT GCGCAACGAA GCGCAACCCA CGCGCTCGCA GCTCGCCGCC GACAGGCAAC TGCAGCGTGC AGTGCGGTCC GGCAAGTTGG GCGCGACCGA CGTCGCGACG CTCACCTTCG AAGCGATCGC GGAACGCCGC TTCTACATCC TCACGCATCC CGCCATTCTC GCGACGGTGC GGCTTCGTCA CGAGGACATC GAACTGCAGC GCAATCCGAC CGATCCGTTG TCGCTGAAGC CCGAAGTCAA GGAAGCCCGC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for BuphA.00010.n.B1.PS37820
| PURIFICATION DATe: | 1/20/2016 |
| CONCENTRATION: | 57.18mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF EFDGKVAVIT GAGSGFGRAF AEKGASLGMK LVLADVDEGA LARTVDTLRA AGAEVIGVRT DVSNGAQVQA LADAALEAFG KVHLLFNNAG VGAGGFLWES SANDWAWVFG VNVMGVAHGV RVFAPIMLGQ NEAAHIVNTA SVAGLLSPPS MGIYNASKHA VVSLTETLYH DLRNAGGEVG CSLLCPAFVP TGIADAERVR PEALRNEAQP TRSQLAADRQ LQRAVRSGKL GATDVATLTF EAIAERRFYI LTHPAILATV RLRHEDIELQ RNPTDPLSLX XXVKEAR
Validated NT Sequence
ttactcactt agcgggcttc cttgacttnn gnnntcagcg acaacggatc ggtcggattg cgctgcagtt cgatgtcctc gtgacgaagc cgcaccgtcg cgagaatggc gggatgcgtg aggatgtaga agcggcgttc cgcgatcgct tcgaaggtga gcgtcgcgac gtcggtcgcg cccaacttgc cggaccgcac tgcacgctgc agttgcctgt cggcggcgag ctgcgagcgc gtgggttgcg cttcgttgcg caacgcctcg gggcgcacgc gttcggcgtc ggcgatacct gtgggcacga acgcgggaca cagcagcgaa cagcccacct cgccgcccgc attgcgcagg tcgtgataca gcgtctcggt cagcgaaacc accgcatgct tcgacgcgtt gtagatgccc atcgacggcg gcgacagcag gcccgccacc gacgccgtat tgacgatatg cgccgcttcg ttctgcccga gcatgatggg cgcgaagacg cgcacgccgt gcgcgacgcc catcacgttg acgccgaaca cccacgccca gtcgttcgcc gagctttccc atagaaagcc gccggcgccg acgcccgcgt tgttgaacag cagatgcacc ttgccgaacg cttcgagcgc cgcatcggcg agcgcctgaa cctgcgcgcc attcgacacg tccgtgcgca cgccgatcac ctcggcgccc gctgcgcgca acgtatcgac agttcgtgcg agcgcgcctt cgtcgacatc cgccagcacg agcttcatgc cgagcgacgc gcccttctcc gcaaacgcac ggccaaagcc gcttcccgcg cctgttatca ccgcgacctt gccgtcgaat tcgaacatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMF EFDGKVAVIT GAGSGFGRAF AEKGASLGMK LVLADVDEGA LARTVDTLRA AGAEVIGVRT DVSNGAQVQA LADAALEAFG KVHLLFNNAG VGAGGFLWES SANDWAWVFG VNVMGVAHGV RVFAPIMLGQ NEAAHIVNTA SVAGLLSPPS MGIYNASKHA VVSLTETLYH DLRNAGGEVG CSLLCPAFVP TGIADAERVR PEALRNEAQP TRSQLAADRQ LQRAVRSGKL GATDVATLTF EAIAERRFYI LTHPAILATV RLRHEDIELQ RNPTDPLSLK PEVKEAR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTCG AATTCGACGG CAAGGTCGCG GTGATAACAG GCGCGGGAAG CGGCTTTGGC CGTGCGTTTG CGGAGAAGGG CGCGTCGCTC GGCATGAAGC TCGTGCTGGC GGATGTCGAC GAAGGCGCGC TCGCACGAAC TGTCGATACG TTGCGCGCAG CGGGCGCCGA GGTGATCGGC GTGCGCACGG ACGTGTCGAA TGGCGCGCAG GTTCAGGCGC TCGCCGATGC GGCGCTCGAA GCGTTCGGCA AGGTGCATCT GCTGTTCAAC AACGCGGGCG TCGGCGCCGG CGGCTTTCTA TGGGAAAGCT CGGCGAACGA CTGGGCGTGG GTGTTCGGCG TCAACGTGAT GGGCGTCGCG CACGGCGTGC GCGTCTTCGC GCCCATCATG CTCGGGCAGA ACGAAGCGGC GCATATCGTC AATACGGCGT CGGTGGCGGG CCTGCTGTCG CCGCCGTCGA TGGGCATCTA CAACGCGTCG AAGCATGCGG TGGTTTCGCT GACCGAGACG CTGTATCACG ACCTGCGCAA TGCGGGCGGC GAGGTGGGCT GTTCGCTGCT GTGTCCCGCG TTCGTGCCCA CAGGTATCGC CGACGCCGAA CGCGTGCGCC CCGAGGCGTT GCGCAACGAA GCGCAACCCA CGCGCTCGCA GCTCGCCGCC GACAGGCAAC TGCAGCGTGC AGTGCGGTCC GGCAAGTTGG GCGCGACCGA CGTCGCGACG CTCACCTTCG AAGCGATCGC GGAACGCCGC TTCTACATCC TCACGCATCC CGCCATTCTC GCGACGGTGC GGCTTCGTCA CGAGGACATC GAACTGCAGC GCAATCCGAC CGATCCGTTG TCGCTGAAGC CCGAAGTCAA GGAAGCCCGC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT