BuphA.00010.g
Short-chain dehydrogenase/reductase SDR
| CENTER ID: | BuphA.00010.g |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00010.g.B1.GE39380 | Full length( BuphA.00010.g ) | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.00010.g.B1.PS37866 | Full length( BuphA.00010.g ) | 1 | 259 |
Structures
5VA8
DEPOSITED: 3/24/2017
DETERMINATION: XRay
CLONE: BuphA.00010.g.B1.GE39380
PROTEIN: BuphA.00010.g.B1.PS37866
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129079 |
| PATRIC ID: | fig|391038.7.peg.2197 |
| RefSeq: | YP_001858286.1 |
| UniProt: | B2JE32 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLGIAGKSA LVCAASKGLG RGCAEALAAE GVNVTIVART PETLDATAAA IRANAGVDVQ AVACDITTPE GRAAALAACP QPDILVNNAG GPPPGDFRNF THDDWIRALE ANMLTPIELI RATIDGMISR GFGRVVNITS SSVKAPIDVL GLSNGARSGL TGFIAGVARK VAPNGVTINN LLPGIFDTDR IAVTFDAAAK AQNISVDEAR KQRMATIPAR RFGTPDEFGR ACAFLCSVHA GYITGQNWLI DGGAYPGTY
NT Sequence
ATGGATCTGG GCATCGCAGG AAAATCCGCG CTCGTGTGCG CGGCGAGCAA GGGGCTGGGG CGCGGCTGCG CCGAGGCGCT CGCGGCCGAA GGCGTGAATG TGACGATCGT CGCGCGCACG CCTGAAACGC TCGACGCGAC GGCGGCCGCA ATCCGCGCGA ACGCGGGCGT CGACGTGCAG GCCGTCGCGT GCGACATCAC CACGCCCGAA GGCCGCGCCG CGGCGCTCGC CGCCTGTCCG CAGCCGGATA TTCTCGTCAA CAATGCAGGC GGGCCGCCGC CCGGCGATTT CCGCAACTTC ACGCACGACG ACTGGATCCG CGCGCTCGAA GCGAACATGC TGACGCCGAT CGAACTGATC CGTGCGACCA TCGACGGGAT GATTTCGCGC GGCTTCGGGC GCGTGGTGAA CATCACGAGT TCGTCGGTGA AAGCGCCCAT CGACGTGCTC GGTCTGTCGA ACGGCGCGCG TTCCGGGCTG ACGGGCTTCA TTGCGGGCGT CGCGCGCAAG GTCGCGCCGA ACGGCGTGAC GATCAACAAC CTGCTGCCGG GTATTTTCGA CACGGACCGC ATTGCCGTGA CGTTCGACGC CGCGGCGAAA GCGCAGAACA TTTCCGTCGA CGAGGCGCGC AAGCAGCGCA TGGCGACGAT TCCCGCGCGC CGCTTCGGCA CGCCAGACGA GTTCGGCCGC GCCTGCGCGT TCCTGTGCAG CGTGCACGCG GGCTATATCA CGGGACAGAA CTGGCTGATC GACGGCGGCG CCTACCCGGG CACGTAT
Details for BuphA.00010.g.B1.GE39380
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMD LGIAGKSALV CAASKGLGRG CAEALAAEGV NVTIVARTPE TLDATAAAIR ANAGVDVQAV ACDITTPEGR AAALAACPQP DILVNNAGGP PPGDFRNFTH DDWIRALEAN MLTPIELIRA TIDGMISRGF GRVVNITSSS VKAPIDVLGL SNGARSGLTG FIAGVARKVA PNGVTINNLL PGIFDTDRIA VTFDAAAKAQ NISVDEARKQ RMATIPARRF GTPDEFGRAC AFLCSVHAGY ITGQNWLIDX XAYPGTY
Validated NT Sequence
atcttactca cttaatacgt gcccgggtag gcgcnnncgt cgatcagcca gttctgtccc gtgatatagc ccgcgtgcac gctgcacagg aacgcgcagg cgcggccgaa ctcgtctggc gtgccgaagc ggcgcgcggg aatcgtcgcc atgcgctgct tgcgcgcctc gtcgacggaa atgttctgcg ctttcgccgc ggcgtcgaac gtcacggcaa tgcggtccgt gtcgaaaata cccggcagca ggttgttgat cgtcacgccg ttcggcgcga ccttgcgcgc gacgcccgca atgaagcccg tcagcccgga acgcgcgccg ttcgacagac cgagcacgtc gatgggcgct ttcaccgacg aactcgtgat gttcaccacg cgcccgaagc cgcgcgaaat catcccgtcg atggtcgcac ggatcagttc gatcggcgtc agcatgttcg cttcgagcgc gcggatccag tcgtcgtgcg tgaagttgcg gaaatcgccg ggcggcggcc cgcctgcatt gttgacgaga atatccggct gcggacaggc ggcgagcgcc gcggcgcggc cttcgggcgt ggtgatgtcg cacgcgacgg cctgcacgtc gacgcccgcg ttcgcgcgga ttgcggccgc cgtcgcgtcg agcgtttcag gcgtgcgcgc gacgatcgtc acattcacgc cttcggccgc gagcgcctcg gcgcagccgc gccccagccc cttgctcgcc gcgcacacga gcgcggattt tcctgcgatg cccagatcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMD LGIAGKSALV CAASKGLGRG CAEALAAEGV NVTIVARTPE TLDATAAAIR ANAGVDVQAV ACDITTPEGR AAALAACPQP DILVNNAGGP PPGDFRNFTH DDWIRALEAN MLTPIELIRA TIDGMISRGF GRVVNITSSS VKAPIDVLGL SNGARSGLTG FIAGVARKVA PNGVTINNLL PGIFDTDRIA VTFDAAAKAQ NISVDEARKQ RMATIPARRF GTPDEFGRAC AFLCSVHAGY ITGQNWLIDG GAYPGTY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc tgggcatcgc aggaaaatcc gcgctcgtgt gcgcggcgag caaggggctg gggcgcggct gcgccgaggc gctcgcggcc gaaggcgtga atgtgacgat cgtcgcgcgc acgcctgaaa cgctcgacgc gacggcggcc gcaatccgcg cgaacgcggg cgtcgacgtg caggccgtcg cgtgcgacat caccacgccc gaaggccgcg ccgcggcgct cgccgcctgt ccgcagccgg atattctcgt caacaatgca ggcgggccgc cgcccggcga tttccgcaac ttcacgcacg acgactggat ccgcgcgctc gaagcgaaca tgctgacgcc gatcgaactg atccgtgcga ccatcgacgg gatgatttcg cgcggcttcg ggcgcgtggt gaacatcacg agttcgtcgg tgaaagcgcc catcgacgtg ctcggtctgt cgaacggcgc gcgttccggg ctgacgggct tcattgcggg cgtcgcgcgc aaggtcgcgc cgaacggcgt gacgatcaac aacctgctgc cgggtatttt cgacacggac cgcattgccg tgacgttcga cgccgcggcg aaagcgcaga acatttccgt cgacgaggcg cgcaagcagc gcatggcgac gattcccgcg cgccgcttcg gcacgccaga cgagttcggc cgcgcctgcg cgttcctgtg cagcgtgcac gcgggctata tcacgggaca gaactggctg atcgacggcg gcgcctaccc gggcacgtat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuphA.00010.g.B1.PS37866
| PURIFICATION DATe: | 3/9/2016 |
| CONCENTRATION: | 60mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD LGIAGKSALV CAASKGLGRG CAEALAAEGV NVTIVARTPE TLDATAAAIR ANAGVDVQAV ACDITTPEGR AAALAACPQP DILVNNAGGP PPGDFRNFTH DDWIRALEAN MLTPIELIRA TIDGMISRGF GRVVNITSSS VKAPIDVLGL SNGARSGLTG FIAGVARKVA PNGVTINNLL PGIFDTDRIA VTFDAAAKAQ NISVDEARKQ RMATIPARRF GTPDEFGRAC AFLCSVHAGY ITGQNWLIDX XAYPGTY
Validated NT Sequence
atcttactca cttaatacgt gcccgggtag gcgcnnncgt cgatcagcca gttctgtccc gtgatatagc ccgcgtgcac gctgcacagg aacgcgcagg cgcggccgaa ctcgtctggc gtgccgaagc ggcgcgcggg aatcgtcgcc atgcgctgct tgcgcgcctc gtcgacggaa atgttctgcg ctttcgccgc ggcgtcgaac gtcacggcaa tgcggtccgt gtcgaaaata cccggcagca ggttgttgat cgtcacgccg ttcggcgcga ccttgcgcgc gacgcccgca atgaagcccg tcagcccgga acgcgcgccg ttcgacagac cgagcacgtc gatgggcgct ttcaccgacg aactcgtgat gttcaccacg cgcccgaagc cgcgcgaaat catcccgtcg atggtcgcac ggatcagttc gatcggcgtc agcatgttcg cttcgagcgc gcggatccag tcgtcgtgcg tgaagttgcg gaaatcgccg ggcggcggcc cgcctgcatt gttgacgaga atatccggct gcggacaggc ggcgagcgcc gcggcgcggc cttcgggcgt ggtgatgtcg cacgcgacgg cctgcacgtc gacgcccgcg ttcgcgcgga ttgcggccgc cgtcgcgtcg agcgtttcag gcgtgcgcgc gacgatcgtc acattcacgc cttcggccgc gagcgcctcg gcgcagccgc gccccagccc cttgctcgcc gcgcacacga gcgcggattt tcctgcgatg cccagatcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMD LGIAGKSALV CAASKGLGRG CAEALAAEGV NVTIVARTPE TLDATAAAIR ANAGVDVQAV ACDITTPEGR AAALAACPQP DILVNNAGGP PPGDFRNFTH DDWIRALEAN MLTPIELIRA TIDGMISRGF GRVVNITSSS VKAPIDVLGL SNGARSGLTG FIAGVARKVA PNGVTINNLL PGIFDTDRIA VTFDAAAKAQ NISVDEARKQ RMATIPARRF GTPDEFGRAC AFLCSVHAGY ITGQNWLIDG GAYPGTY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATC TGGGCATCGC AGGAAAATCC GCGCTCGTGT GCGCGGCGAG CAAGGGGCTG GGGCGCGGCT GCGCCGAGGC GCTCGCGGCC GAAGGCGTGA ATGTGACGAT CGTCGCGCGC ACGCCTGAAA CGCTCGACGC GACGGCGGCC GCAATCCGCG CGAACGCGGG CGTCGACGTG CAGGCCGTCG CGTGCGACAT CACCACGCCC GAAGGCCGCG CCGCGGCGCT CGCCGCCTGT CCGCAGCCGG ATATTCTCGT CAACAATGCA GGCGGGCCGC CGCCCGGCGA TTTCCGCAAC TTCACGCACG ACGACTGGAT CCGCGCGCTC GAAGCGAACA TGCTGACGCC GATCGAACTG ATCCGTGCGA CCATCGACGG GATGATTTCG CGCGGCTTCG GGCGCGTGGT GAACATCACG AGTTCGTCGG TGAAAGCGCC CATCGACGTG CTCGGTCTGT CGAACGGCGC GCGTTCCGGG CTGACGGGCT TCATTGCGGG CGTCGCGCGC AAGGTCGCGC CGAACGGCGT GACGATCAAC AACCTGCTGC CGGGTATTTT CGACACGGAC CGCATTGCCG TGACGTTCGA CGCCGCGGCG AAAGCGCAGA ACATTTCCGT CGACGAGGCG CGCAAGCAGC GCATGGCGAC GATTCCCGCG CGCCGCTTCG GCACGCCAGA CGAGTTCGGC CGCGCCTGCG CGTTCCTGTG CAGCGTGCAC GCGGGCTATA TCACGGGACA GAACTGGCTG ATCGACGGCG GCGCCTACCC GGGCACGTAT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT