BumuA.17230.b

Putative transketolase

CENTER ID: BumuA.17230.b
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.17230.b.A1.GE31963 Full length( BumuA.17230.b ) 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.5316
RefSeq: YP_001584954.1
UniProt: A0A0H3KJJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTAAQKPRL KTSAMIASIA AEGQPTRAAP FGHALVELAR TKPDVIGMTA DLGKYTDLHV FAKAFPERYY QMGMAEQLLM GAAAGFAHEG AQPFVTTYAV FATRRAYDFI HQTIAEDNLD VKIVAALPGL TTGYGPSHQA AEDLALMRAM PNMTVIDPCD ALDIEQMVPA IAAHDGPVYA RLLRGNVPVV LDEYDYTFEL GKAKRLRDGN DVLLISSGIM TMRALEVANA LEADRVGVAV LHVPTIKPLD TATIVREAKR TGRMVVVAEN HTVIGGLGEA VARTLLAEGV APTFRQIALP DAFLDAGALP TLHDRYGIST ATMVASIKRW LG
NT Sequence
ATGAGTACCG CCGCCCAGAA GCCGCGCCTG AAAACCTCCG CGATGATCGC ATCGATCGCG GCCGAAGGAC AGCCGACGCG CGCCGCACCG TTCGGTCACG CCCTCGTCGA GCTCGCGCGC ACGAAGCCCG ACGTGATCGG CATGACGGCG GATCTCGGCA AGTACACGGA CCTGCACGTA TTCGCGAAGG CGTTTCCCGA GCGCTACTAC CAGATGGGGA TGGCCGAGCA GCTGTTGATG GGAGCCGCGG CCGGCTTTGC GCACGAAGGT GCGCAGCCGT TCGTGACGAC CTATGCGGTG TTCGCGACGC GCCGGGCGTA CGACTTCATC CATCAGACGA TCGCCGAGGA CAATCTCGAC GTGAAGATCG TCGCCGCGCT GCCGGGACTG ACGACGGGCT ACGGCCCGAG CCACCAGGCC GCGGAAGATC TCGCGCTGAT GCGCGCGATG CCGAACATGA CCGTGATCGA TCCGTGCGAC GCGCTCGACA TCGAGCAGAT GGTGCCCGCG ATCGCGGCGC ACGACGGCCC CGTGTATGCG CGGCTGCTGC GCGGCAACGT GCCGGTCGTG CTCGACGAAT ACGACTACAC GTTCGAGCTC GGCAAGGCGA AACGGCTGCG CGACGGCAAC GACGTACTGC TGATCTCGTC CGGCATCATG ACAATGCGCG CGCTCGAAGT GGCGAACGCG CTGGAGGCCG ATCGCGTCGG CGTCGCGGTG CTGCACGTGC CGACGATCAA GCCGCTCGAT ACCGCGACGA TCGTGCGCGA AGCGAAGCGC ACGGGCCGCA TGGTCGTCGT CGCGGAAAAC CACACGGTGA TCGGCGGGCT CGGCGAAGCC GTCGCGCGCA CACTGCTTGC CGAAGGCGTC GCGCCGACGT TCCGGCAGAT CGCGCTGCCC GATGCGTTCC TCGACGCGGG CGCGCTGCCG ACGCTGCACG ATCGCTACGG CATCTCGACC GCCACGATGG TCGCGTCGAT CAAGCGCTGG CTCGGC
Details for BumuA.17230.b.A1.GE31963
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMSTAAQK PRLKTSAMIA SIAAEGQPTR AAPFGHALVE LARTKPDVIG MTADLGKYTD LHVFAKAFPE RYYQMGMAEQ LLMGAAAGFA HEGAQPFVTT YAVFATRRAY DFIHQTIAED NLDVKIVAAL PGLTTGYGPS HQAAEDLALM RAMPNMTVID PCDALDIEQM VPAIAAHDGP VYARLLRGNV PVVLDEYDYT FELGKAKRLR DGNDVLLISS GIMTMRALEV ANALEADRVG VAVLHVPTIK PLDTATIVRE AKRTGRMVVV AENHTVIGGL GEAVARTLLA EGVAPTFRQI ALPDAFLDAG ALPTLHDRYG ISTATMVASI KRW
Validated NT Sequence
agccagcgct tgatcgacgc gaccatcgtg gcggtcgaga tgccgtagcg atcgtgcagc gtcggcagcg cgcccgcgtc gaggaacgca tcgggcagcg cgatctgccg gaacgtcggc gcgacgcctt cggcaagcag tgtgcgcgcg acggcttcgc cgagcccgcc gatcaccgtg tggttttccg cgacgacgac catgcggccc gtgcgcttcg cttcgcgcac gatcgtcgcg gtatcgagcg gcttgatcgt cggcacgtgc agcaccgcga cgccgacgcg atcggcctcc agcgcgttcg ccacttcgag cgcgcgcatt gtcatgatgc cggacgagat cagcagtacg tcgttgccgt cgcgcagccg tttcgccttg ccgagctcga acgtgtagtc gtattcgtcg agcacgaccg gcacgttgcc gcgcagcagc cgcgcataca cggggccgtc gtgcgccgcg atcgcgggca ccatctgctc gatgtcgagc gcgtcgcacg gatcgatcac ggtcatgttc ggcatcgcgc gcatcagcgc gagatcttcc gcggcctggt ggctcgggcc gtagcccgtc gtcagtcccg gcagcgcggc gacgatcttc acgtcgagat tgtcctcggc gatcgtctga tggatgaagt cgtacgcccg gcgcgtcgcg aacaccgcat aggtcgtcac gaacggctgc gcaccttcgt gcgcaaagcc ggccgcggct cccatcaaca gctgctcggc catccccatc tggtagtagc gctcgggaaa cgccttcgcg aatacgtgca ggtccgtgta cttgccgaga tccgccgtca tgccgatcac gtcgggcttc gtgcgcgcga gctcgacgag ggcgtgaccg aacggtgcgg cgcgcgtcgg ctgtccttcg gccgcgatcg atgcgatcat cgcggaggtt ttcaggcgcg gcttctgggc ggcggtactc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTAAQKPR LKTSAMIASI AAEGQPTRAA PFGHALVELA RTKPDVIGMT ADLGKYTDLH VFAKAFPERY YQMGMAEQLL MGAAAGFAHE GAQPFVTTYA VFATRRAYDF IHQTIAEDNL DVKIVAALPG LTTGYGPSHQ AAEDLALMRA MPNMTVIDPC DALDIEQMVP AIAAHDGPVY ARLLRGNVPV VLDEYDYTFE LGKAKRLRDG NDVLLISSGI MTMRALEVAN ALEADRVGVA VLHVPTIKPL DTATIVREAK RTGRMVVVAE NHTVIGGLGE AVARTLLAEG VAPTFRQIAL PDAFLDAGAL PTLHDRYGIS TATMVASIKR WLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtaccgcc gcccagaagc cgcgcctgaa aacctccgcg atgatcgcat cgatcgcggc cgaaggacag ccgacgcgcg ccgcaccgtt cggtcacgcc ctcgtcgagc tcgcgcgcac gaagcccgac gtgatcggca tgacggcgga tctcggcaag tacacggacc tgcacgtatt cgcgaaggcg tttcccgagc gctactacca gatggggatg gccgagcagc tgttgatggg agccgcggcc ggctttgcgc acgaaggtgc gcagccgttc gtgacgacct atgcggtgtt cgcgacgcgc cgggcgtacg acttcatcca tcagacgatc gccgaggaca atctcgacgt gaagatcgtc gccgcgctgc cgggactgac gacgggctac ggcccgagcc accaggccgc ggaagatctc gcgctgatgc gcgcgatgcc gaacatgacc gtgatcgatc cgtgcgacgc gctcgacatc gagcagatgg tgcccgcgat cgcggcgcac gacggccccg tgtatgcgcg gctgctgcgc ggcaacgtgc cggtcgtgct cgacgaatac gactacacgt tcgagctcgg caaggcgaaa cggctgcgcg acggcaacga cgtactgctg atctcgtccg gcatcatgac aatgcgcgcg ctcgaagtgg cgaacgcgct ggaggccgat cgcgtcggcg tcgcggtgct gcacgtgccg acgatcaagc cgctcgatac cgcgacgatc gtgcgcgaag cgaagcgcac gggccgcatg gtcgtcgtcg cggaaaacca cacggtgatc ggcgggctcg gcgaagccgt cgcgcgcaca ctgcttgccg aaggcgtcgc gccgacgttc cggcagatcg cgctgcccga tgcgttcctc gacgcgggcg cgctgccgac gctgcacgat cgctacggca tctcgaccgc cacgatggtc gcgtcgatca agcgctggct cggctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa