BumuA.17069.a

Putative uncharacterized protein

CENTER ID: BumuA.17069.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.17069.a.A2.GE34666 Mature protein( BumuA.17069.a ) 32 265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.17069.a.A2.PW35276 Mature protein( BumuA.17069.a ) 32 265

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.1020
RefSeq: YP_001579054.1
UniProt: A0A0H3KH44

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAQGRIQAV RAAAAALAVW AVCSAAPGLA HAEVAAADPI DVAMRQCLAR RDRSSTAGQI QCMNEAQQQW QAAMDAAYAR LLKDAPADAK RGWQESQRRW LTWRKDEAQL LKAVYDTTSG TMYTMASADM QLQPVRSRAL ALRAAAERYA PPAAIPAAVG SGAQAAAAPA NGKPANVTRD APIGRVRPCD QDAACEHAVF DLNRYYQKLR RKMPAHSAAT LVRAQRAWFA FRDATAPLVG EDGRVDLIGA RIATMKRLSE TAGNR
NT Sequence
ATGGCGGCGC AGGGACGAAT CCAGGCGGTG CGCGCGGCGG CCGCCGCGCT CGCCGTCTGG GCGGTCTGCT CGGCGGCGCC GGGCCTCGCG CACGCCGAAG TCGCGGCGGC CGATCCGATC GACGTCGCGA TGCGGCAGTG CCTCGCGCGG CGCGACCGTT CGTCGACGGC CGGCCAGATC CAGTGCATGA ACGAAGCGCA GCAGCAGTGG CAGGCCGCGA TGGACGCCGC CTACGCGCGC CTGCTGAAGG ACGCGCCGGC CGACGCGAAG CGCGGCTGGC AGGAGAGCCA GCGGCGCTGG CTCACGTGGC GCAAGGACGA AGCGCAACTG CTGAAGGCCG TCTACGACAC GACGAGCGGC ACGATGTACA CGATGGCGAG CGCCGACATG CAGCTGCAGC CCGTGCGCAG CCGCGCGCTC GCGCTGCGTG CCGCGGCCGA GCGCTATGCG CCGCCTGCCG CGATACCGGC GGCGGTCGGA AGCGGCGCGC AGGCCGCTGC GGCGCCGGCG AACGGCAAGC CGGCGAACGT GACGCGCGAT GCGCCGATCG GCCGCGTGCG GCCGTGCGAC CAGGACGCCG CGTGCGAGCA TGCGGTGTTC GATCTGAACC GCTATTACCA GAAGCTGCGC CGCAAGATGC CCGCGCATTC GGCCGCGACG CTCGTGCGTG CGCAGCGCGC GTGGTTCGCG TTTCGCGATG CGACCGCGCC GCTCGTCGGC GAAGACGGAC GCGTCGATCT GATCGGCGCG CGCATCGCCA CGATGAAGCG GCTGTCCGAG ACGGCCGGCA ACCGT
Details for BumuA.17069.a.A2.GE34666
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 27kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEVAAADP IDVAMRQCLA RRDRSSTAGQ IQCMNEAQQQ WQAAMDAAYA RLLKDAPADA KRGWQESQRR WLTWRKDEAQ LLKAVYDTTS GTMYTMASAD MQLQPVRSRA LALRAAAERY APPAAIPAAV GSGAQAAAAP ANGKPANVTR DAPIGRVRPC DQDAACEHAV FDLNRYYQKL RRKMPAHSAA TLVRAQRAWF AFRDATAPLV GEDGRVXLIG ARIATMKRLS ETAG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg aagtcgcggc ggccgatccg atcgacgtcg cgatgcggca gtgcctcgcg cggcgcgacc gttcgtcgac ggccggccag atccagtgca tgaacgaagc gcagcagcag tggcaggccg cgatggacgc cgcctacgcg cgcctgctga aggacgcgcc ggccgacgcg aagcgcggct ggcaggagag ccagcggcgc tggctcacgt ggcgcaagga cgaagcgcaa ctgctgaagg ccgtctacga cacgacgagc ggcacgatgt acacgatggc gagcgccgac atgcagctgc agcccgtgcg cagccgcgcg ctcgcgctgc gtgccgcggc cgagcgctat gcgccgcctg ccgcgatacc ggcggcggtc ggaagcggcg cgcaggccgc tgcggcgccg gcgaacggca agccggcgaa cgtgacgcgc gatgcgccga tcggccgcgt gcggccgtgc gaccaggacg ccgcgtgcga gcatgcggtg ttcgatctga accgctatta ccagaagctg cgccgcaaga tgcccgcgca ttcggccgcg acgctcgtgc gtgcgcagcg cgcgtggttc gcgtttcgcg atgcgaccgc gccgctcgtc ggcgaagacg gacgcgtcna tctgatcggc gcgcgcatcg ccacnatgaa gcggctgtcc gagacggccg gca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEVAAADP IDVAMRQCLA RRDRSSTAGQ IQCMNEAQQQ WQAAMDAAYA RLLKDAPADA KRGWQESQRR WLTWRKDEAQ LLKAVYDTTS GTMYTMASAD MQLQPVRSRA LALRAAAERY APPAAIPAAV GSGAQAAAAP ANGKPANVTR DAPIGRVRPC DQDAACEHAV FDLNRYYQKL RRKMPAHSAA TLVRAQRAWF AFRDATAPLV GEDGRVDLIG ARIATMKRLS ETAGNR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgaagtc gcggcggccg atccgatcga cgtcgcgatg cggcagtgcc tcgcgcggcg cgaccgttcg tcgacggccg gccagatcca gtgcatgaac gaagcgcagc agcagtggca ggccgcgatg gacgccgcct acgcgcgcct gctgaaggac gcgccggccg acgcgaagcg cggctggcag gagagccagc ggcgctggct cacgtggcgc aaggacgaag cgcaactgct gaaggccgtc tacgacacga cgagcggcac gatgtacacg atggcgagcg ccgacatgca gctgcagccc gtgcgcagcc gcgcgctcgc gctgcgtgcc gcggccgagc gctatgcgcc gcctgccgcg ataccggcgg cggtcggaag cggcgcgcag gccgctgcgg cgccggcgaa cggcaagccg gcgaacgtga cgcgcgatgc gccgatcggc cgcgtgcggc cgtgcgacca ggacgccgcg tgcgagcatg cggtgttcga tctgaaccgc tattaccaga agctgcgccg caagatgccc gcgcattcgg ccgcgacgct cgtgcgtgcg cagcgcgcgt ggttcgcgtt tcgcgatgcg accgcgccgc tcgtcggcga agacggacgc gtcgatctga tcggcgcgcg catcgccacg atgaagcggc tgtccgagac ggccggcaac cgttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BumuA.17069.a.A2.PW35276
PURIFICATION DATe: 4/13/2012
CONCENTRATION: 37.47mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEVAAADP IDVAMRQCLA RRDRSSTAGQ IQCMNEAQQQ WQAAMDAAYA RLLKDAPADA KRGWQESQRR WLTWRKDEAQ LLKAVYDTTS GTMYTMASAD MQLQPVRSRA LALRAAAERY APPAAIPAAV GSGAQAAAAP ANGKPANVTR DAPIGRVRPC DQDAACEHAV FDLNRYYQKL RRKMPAHSAA TLVRAQRAWF AFRDATAPLV GEDGRVXLIG ARIATMKRLS ETAG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg aagtcgcggc ggccgatccg atcgacgtcg cgatgcggca gtgcctcgcg cggcgcgacc gttcgtcgac ggccggccag atccagtgca tgaacgaagc gcagcagcag tggcaggccg cgatggacgc cgcctacgcg cgcctgctga aggacgcgcc ggccgacgcg aagcgcggct ggcaggagag ccagcggcgc tggctcacgt ggcgcaagga cgaagcgcaa ctgctgaagg ccgtctacga cacgacgagc ggcacgatgt acacgatggc gagcgccgac atgcagctgc agcccgtgcg cagccgcgcg ctcgcgctgc gtgccgcggc cgagcgctat gcgccgcctg ccgcgatacc ggcggcggtc ggaagcggcg cgcaggccgc tgcggcgccg gcgaacggca agccggcgaa cgtgacgcgc gatgcgccga tcggccgcgt gcggccgtgc gaccaggacg ccgcgtgcga gcatgcggtg ttcgatctga accgctatta ccagaagctg cgccgcaaga tgcccgcgca ttcggccgcg acgctcgtgc gtgcgcagcg cgcgtggttc gcgtttcgcg atgcgaccgc gccgctcgtc ggcgaagacg gacgcgtcna tctgatcggc gcgcgcatcg ccacnatgaa gcggctgtcc gagacggccg gca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEVAAADP IDVAMRQCLA RRDRSSTAGQ IQCMNEAQQQ WQAAMDAAYA RLLKDAPADA KRGWQESQRR WLTWRKDEAQ LLKAVYDTTS GTMYTMASAD MQLQPVRSRA LALRAAAERY APPAAIPAAV GSGAQAAAAP ANGKPANVTR DAPIGRVRPC DQDAACEHAV FDLNRYYQKL RRKMPAHSAA TLVRAQRAWF AFRDATAPLV GEDGRVDLIG ARIATMKRLS ETAGNR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGAAGTC GCGGCGGCCG ATCCGATCGA CGTCGCGATG CGGCAGTGCC TCGCGCGGCG CGACCGTTCG TCGACGGCCG GCCAGATCCA GTGCATGAAC GAAGCGCAGC AGCAGTGGCA GGCCGCGATG GACGCCGCCT ACGCGCGCCT GCTGAAGGAC GCGCCGGCCG ACGCGAAGCG CGGCTGGCAG GAGAGCCAGC GGCGCTGGCT CACGTGGCGC AAGGACGAAG CGCAACTGCT GAAGGCCGTC TACGACACGA CGAGCGGCAC GATGTACACG ATGGCGAGCG CCGACATGCA GCTGCAGCCC GTGCGCAGCC GCGCGCTCGC GCTGCGTGCC GCGGCCGAGC GCTATGCGCC GCCTGCCGCG ATACCGGCGG CGGTCGGAAG CGGCGCGCAG GCCGCTGCGG CGCCGGCGAA CGGCAAGCCG GCGAACGTGA CGCGCGATGC GCCGATCGGC CGCGTGCGGC CGTGCGACCA GGACGCCGCG TGCGAGCATG CGGTGTTCGA TCTGAACCGC TATTACCAGA AGCTGCGCCG CAAGATGCCC GCGCATTCGG CCGCGACGCT CGTGCGTGCG CAGCGCGCGT GGTTCGCGTT TCGCGATGCG ACCGCGCCGC TCGTCGGCGA AGACGGACGC GTCGATCTGA TCGGCGCGCG CATCGCCACG ATGAAGCGGC TGTCCGAGAC GGCCGGCAAC CGTTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA