BumuA.15997.a
Beta-lactamase domain protein
| CENTER ID: | BumuA.15997.a |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.15997.a.B1.GE39716 | Full length( BumuA.15997.a ) | 1 | 359 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.15997.a.B1.PS37897 | Full length( BumuA.15997.a ) | 1 | 359 |
Structures
5U8O
DEPOSITED: 12/14/2016
DETERMINATION: XRay
CLONE: BumuA.15997.a.B1.GE39716
PROTEIN: BumuA.15997.a.B1.PS37897
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_155546 |
| PATRIC ID: | fig|395019.8.peg.513 |
| RefSeq: | YP_001578572.1 |
| UniProt: | A0A0H3KRR5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNALEHQLDY PFADTLPAAG DTFEVAPGVR WLRMPLPFSL DHINLWLLRD EIDGQAGWTV VDCGIASDAI RTHWEQIFDA HLDGLPVLRV IVTHCHPDHF GLANWLCEGG DKRRWNVRLW MTLGEYMFGC LMAAGNGSNA GGAAAADHFA RHGLTDAASL EKLRNRRSYY SDLVPAVPPR YRRLRDGDAV TIGTRTWRVV TGYGHSPEHC ALHSEADGVL ISGDMVLPRI STNVSVFDLE PEANPLALYL QSLGRYETMA PDTLVLPSHG KPFRGVRTRI AQLRAHHDAR LDEVRVACAE QPMSAADIVP IMFRRRELDI HQMTFALGEA IAHLNLLWLA GELVREQGDD GVLRFRIAR
NT Sequence
ATGAACGCAC TGGAACACCA ACTCGACTAT CCGTTCGCCG ACACGCTGCC CGCCGCCGGC GACACGTTCG AGGTCGCGCC CGGCGTGCGC TGGCTGCGCA TGCCGCTGCC GTTTTCGCTC GATCACATCA ACCTGTGGCT GCTGCGCGAC GAGATCGACG GGCAGGCCGG CTGGACCGTG GTCGACTGCG GGATCGCATC GGACGCGATC CGCACGCACT GGGAACAGAT CTTCGACGCG CACCTCGACG GCCTGCCGGT GCTGCGCGTG ATCGTCACGC ACTGCCATCC CGATCACTTC GGGCTCGCGA ACTGGCTCTG CGAGGGCGGC GACAAGCGGC GCTGGAACGT GCGGCTATGG ATGACGCTCG GCGAATACAT GTTCGGCTGC CTGATGGCGG CCGGCAACGG ATCGAACGCG GGCGGCGCGG CCGCGGCCGA CCATTTCGCG CGCCACGGGC TGACGGACGC CGCGTCGCTC GAGAAGCTGC GCAATCGCCG CAGCTATTAC TCGGACCTCG TGCCGGCCGT GCCGCCGCGC TACCGGCGCC TGCGCGACGG CGACGCCGTG ACGATCGGCA CGCGCACGTG GCGCGTCGTG ACGGGCTACG GCCATTCGCC TGAACATTGC GCGCTGCACA GCGAAGCGGA CGGCGTGCTG ATCTCCGGCG ACATGGTGCT GCCGCGGATC TCGACGAACG TGTCGGTGTT CGATCTGGAG CCGGAGGCGA ATCCGCTCGC GCTCTATCTG CAGTCGCTCG GCCGCTACGA GACGATGGCG CCCGACACGC TCGTGCTGCC GTCGCACGGC AAGCCGTTTC GCGGCGTGCG CACGCGCATC GCGCAACTGC GCGCGCATCA CGACGCGCGG CTCGACGAAG TGCGCGTCGC GTGCGCGGAA CAGCCGATGA GCGCGGCCGA CATCGTGCCG ATCATGTTCC GCCGCCGCGA GCTCGACATC CATCAGATGA CGTTCGCCCT CGGCGAAGCG ATCGCCCACC TGAACCTGCT GTGGCTCGCC GGCGAACTCG TACGCGAGCA GGGCGACGAC GGCGTGCTGC GGTTCCGCAT CGCGCGC
Details for BumuA.15997.a.B1.GE39716
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMN ALEHQLDYPF ADTLPAAGDT FEVAPGVRWL RMPLPFSLDH INLWLLRDEI DGQAGWTVVD CGIASDAIRT HWEQIFDAHL DGLPVLRVIV THCHPDHFGL ANWLCEGGDK RRWNVRLWMT LGEYMFGCLM AAGNGSNAGG AAAADHFARH GLTDAASLEK LRNRRSYYSD LVPAVPPRYR RLRDGDAVTI GTRTWRVVTG YGHSPEHCAL HSEADGVLIS GDMVLPRIST NVSVFDLEPE ANPLALYLQS LGRYETMAPD TLVLPSHGKP FRGVRTRIAQ LRAHHDARLD EVRVACAEQP MSAADIVPIM FRRRELDIHQ MTFALGEAIA HLNLLWLAGE LVREQGDD
Validated NT Sequence
cgtcgtcgcc ctgctcgcgt acgagttcgc cggcgagcca cagcaggttc aggtgggcga tcgcttcgcc gagggcgaac gtcatctgat ggatgtcgag ctcgcggcgg cggaacatga tcggcacgat gtcggccgcg ctcatcggct gttccgcgca cgcgacgcgc acttcgtcga gccgcgcgtc gtgatgcgcg cgcagttgcg cgatgcgcgt gcgcacgccg cgaaacggct tgccgtgcga cggcagcacg agcgtgtcgg gcgccatcgt ctcgtagcgg ccgagcgact gcagatagag cgcgagcgga ttcgcctccg gctccagatc gaacaccgac acgttcgtcg agatccgcgg cagcaccatg tcgccggaga tcagcacgcc gtccgcttcg ctgtgcagcg cgcaatgttc aggcgaatgg ccgtagcccg tcacgacgcg ccacgtgcgc gtgccgatcg tcacggcgtc gccgtcgcgc aggcgccggt agcgcggcgg cacggccggc acgaggtccg agtaatagct gcggcgattg cgcagcttct cgagcgacgc ggcgtccgtc agcccgtggc gcgcgaaatg gtcggccgcg gccgcgccgc ccgcgttcga tccgttgccg gccgccatca ggcagccgaa catgtattcg ccgagcgtca tccatagccg cacgttccag cgccgcttgt cgccgccctc gcagagccag ttcgcgagcc cgaagtgatc gggatggcag tgcgtgacga tcacgcgcag caccggcagg ccgtcgaggt gcgcgtcgaa gatctgttcc cagtgcgtgc ggatcgcgtc cgatgcgatc ccgcagtcga ccacggtcca gccggcctgc ccgtcgatct cgtcgcgcag cagccacagg ttgatgtgat cgagcgaaaa cggcagcggc atgcgcagcc agcgcacgcc gggcgcgacc tcgaacgtgt cgccggcggc gggcagcgtg tcggcgaacg gatagtcgag ttggtgttcc agtgcgttca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMN ALEHQLDYPF ADTLPAAGDT FEVAPGVRWL RMPLPFSLDH INLWLLRDEI DGQAGWTVVD CGIASDAIRT HWEQIFDAHL DGLPVLRVIV THCHPDHFGL ANWLCEGGDK RRWNVRLWMT LGEYMFGCLM AAGNGSNAGG AAAADHFARH GLTDAASLEK LRNRRSYYSD LVPAVPPRYR RLRDGDAVTI GTRTWRVVTG YGHSPEHCAL HSEADGVLIS GDMVLPRIST NVSVFDLEPE ANPLALYLQS LGRYETMAPD TLVLPSHGKP FRGVRTRIAQ LRAHHDARLD EVRVACAEQP MSAADIVPIM FRRRELDIHQ MTFALGEAIA HLNLLWLAGE LVREQGDDGV LRFRIAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg cactggaaca ccaactcgac tatccgttcg ccgacacgct gcccgccgcc ggcgacacgt tcgaggtcgc gcccggcgtg cgctggctgc gcatgccgct gccgttttcg ctcgatcaca tcaacctgtg gctgctgcgc gacgagatcg acgggcaggc cggctggacc gtggtcgact gcgggatcgc atcggacgcg atccgcacgc actgggaaca gatcttcgac gcgcacctcg acggcctgcc ggtgctgcgc gtgatcgtca cgcactgcca tcccgatcac ttcgggctcg cgaactggct ctgcgagggc ggcgacaagc ggcgctggaa cgtgcggcta tggatgacgc tcggcgaata catgttcggc tgcctgatgg cggccggcaa cggatcgaac gcgggcggcg cggccgcggc cgaccatttc gcgcgccacg ggctgacgga cgccgcgtcg ctcgagaagc tgcgcaatcg ccgcagctat tactcggacc tcgtgccggc cgtgccgccg cgctaccggc gcctgcgcga cggcgacgcc gtgacgatcg gcacgcgcac gtggcgcgtc gtgacgggct acggccattc gcctgaacat tgcgcgctgc acagcgaagc ggacggcgtg ctgatctccg gcgacatggt gctgccgcgg atctcgacga acgtgtcggt gttcgatctg gagccggagg cgaatccgct cgcgctctat ctgcagtcgc tcggccgcta cgagacgatg gcgcccgaca cgctcgtgct gccgtcgcac ggcaagccgt ttcgcggcgt gcgcacgcgc atcgcgcaac tgcgcgcgca tcacgacgcg cggctcgacg aagtgcgcgt cgcgtgcgcg gaacagccga tgagcgcggc cgacatcgtg ccgatcatgt tccgccgccg cgagctcgac atccatcaga tgacgttcgc cctcggcgaa gcgatcgccc acctgaacct gctgtggctc gccggcgaac tcgtacgcga gcagggcgac gacggcgtgc tgcggttccg catcgcgcgc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BumuA.15997.a.B1.PS37897
| PURIFICATION DATe: | 4/5/2016 |
| CONCENTRATION: | 44.9mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN ALEHQLDYPF ADTLPAAGDT FEVAPGVRWL RMPLPFSLDH INLWLLRDEI DGQAGWTVVD CGIASDAIRT HWEQIFDAHL DGLPVLRVIV THCHPDHFGL ANWLCEGGDK RRWNVRLWMT LGEYMFGCLM AAGNGSNAGG AAAADHFARH GLTDAASLEK LRNRRSYYSD LVPAVPPRYR RLRDGDAVTI GTRTWRVVTG YGHSPEHCAL HSEADGVLIS GDMVLPRIST NVSVFDLEPE ANPLALYLQS LGRYETMAPD TLVLPSHGKP FRGVRTRIAQ LRAHHDARLD EVRVACAEQP MSAADIVPIM FRRRELDIHQ MTFALGEAIA HLNLLWLAGE LVREQGDD
Validated NT Sequence
cgtcgtcgcc ctgctcgcgt acgagttcgc cggcgagcca cagcaggttc aggtgggcga tcgcttcgcc gagggcgaac gtcatctgat ggatgtcgag ctcgcggcgg cggaacatga tcggcacgat gtcggccgcg ctcatcggct gttccgcgca cgcgacgcgc acttcgtcga gccgcgcgtc gtgatgcgcg cgcagttgcg cgatgcgcgt gcgcacgccg cgaaacggct tgccgtgcga cggcagcacg agcgtgtcgg gcgccatcgt ctcgtagcgg ccgagcgact gcagatagag cgcgagcgga ttcgcctccg gctccagatc gaacaccgac acgttcgtcg agatccgcgg cagcaccatg tcgccggaga tcagcacgcc gtccgcttcg ctgtgcagcg cgcaatgttc aggcgaatgg ccgtagcccg tcacgacgcg ccacgtgcgc gtgccgatcg tcacggcgtc gccgtcgcgc aggcgccggt agcgcggcgg cacggccggc acgaggtccg agtaatagct gcggcgattg cgcagcttct cgagcgacgc ggcgtccgtc agcccgtggc gcgcgaaatg gtcggccgcg gccgcgccgc ccgcgttcga tccgttgccg gccgccatca ggcagccgaa catgtattcg ccgagcgtca tccatagccg cacgttccag cgccgcttgt cgccgccctc gcagagccag ttcgcgagcc cgaagtgatc gggatggcag tgcgtgacga tcacgcgcag caccggcagg ccgtcgaggt gcgcgtcgaa gatctgttcc cagtgcgtgc ggatcgcgtc cgatgcgatc ccgcagtcga ccacggtcca gccggcctgc ccgtcgatct cgtcgcgcag cagccacagg ttgatgtgat cgagcgaaaa cggcagcggc atgcgcagcc agcgcacgcc gggcgcgacc tcgaacgtgt cgccggcggc gggcagcgtg tcggcgaacg gatagtcgag ttggtgttcc agtgcgttca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMN ALEHQLDYPF ADTLPAAGDT FEVAPGVRWL RMPLPFSLDH INLWLLRDEI DGQAGWTVVD CGIASDAIRT HWEQIFDAHL DGLPVLRVIV THCHPDHFGL ANWLCEGGDK RRWNVRLWMT LGEYMFGCLM AAGNGSNAGG AAAADHFARH GLTDAASLEK LRNRRSYYSD LVPAVPPRYR RLRDGDAVTI GTRTWRVVTG YGHSPEHCAL HSEADGVLIS GDMVLPRIST NVSVFDLEPE ANPLALYLQS LGRYETMAPD TLVLPSHGKP FRGVRTRIAQ LRAHHDARLD EVRVACAEQP MSAADIVPIM FRRRELDIHQ MTFALGEAIA HLNLLWLAGE LVREQGDDGV LRFRIAR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG CACTGGAACA CCAACTCGAC TATCCGTTCG CCGACACGCT GCCCGCCGCC GGCGACACGT TCGAGGTCGC GCCCGGCGTG CGCTGGCTGC GCATGCCGCT GCCGTTTTCG CTCGATCACA TCAACCTGTG GCTGCTGCGC GACGAGATCG ACGGGCAGGC CGGCTGGACC GTGGTCGACT GCGGGATCGC ATCGGACGCG ATCCGCACGC ACTGGGAACA GATCTTCGAC GCGCACCTCG ACGGCCTGCC GGTGCTGCGC GTGATCGTCA CGCACTGCCA TCCCGATCAC TTCGGGCTCG CGAACTGGCT CTGCGAGGGC GGCGACAAGC GGCGCTGGAA CGTGCGGCTA TGGATGACGC TCGGCGAATA CATGTTCGGC TGCCTGATGG CGGCCGGCAA CGGATCGAAC GCGGGCGGCG CGGCCGCGGC CGACCATTTC GCGCGCCACG GGCTGACGGA CGCCGCGTCG CTCGAGAAGC TGCGCAATCG CCGCAGCTAT TACTCGGACC TCGTGCCGGC CGTGCCGCCG CGCTACCGGC GCCTGCGCGA CGGCGACGCC GTGACGATCG GCACGCGCAC GTGGCGCGTC GTGACGGGCT ACGGCCATTC GCCTGAACAT TGCGCGCTGC ACAGCGAAGC GGACGGCGTG CTGATCTCCG GCGACATGGT GCTGCCGCGG ATCTCGACGA ACGTGTCGGT GTTCGATCTG GAGCCGGAGG CGAATCCGCT CGCGCTCTAT CTGCAGTCGC TCGGCCGCTA CGAGACGATG GCGCCCGACA CGCTCGTGCT GCCGTCGCAC GGCAAGCCGT TTCGCGGCGT GCGCACGCGC ATCGCGCAAC TGCGCGCGCA TCACGACGCG CGGCTCGACG AAGTGCGCGT CGCGTGCGCG GAACAGCCGA TGAGCGCGGC CGACATCGTG CCGATCATGT TCCGCCGCCG CGAGCTCGAC ATCCATCAGA TGACGTTCGC CCTCGGCGAA GCGATCGCCC ACCTGAACCT GCTGTGGCTC GCCGGCGAAC TCGTACGCGA GCAGGGCGAC GACGGCGTGC TGCGGTTCCG CATCGCGCGC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT