SSGCID: Target Page

BumuA.12800.y

LysR family transcriptional regulator

CENTER ID:	BumuA.12800.y
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.y.A1.GE34673	Full length( BumuA.12800.y )		1	317

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.5669
RefSeq:	YP_001585289.1
UniProt:	A0A0H3KQ53

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLQMMRIF VRVAEEGSFT SAAQRLDITT AYASRSVAQL ETHLRTRLLN RSTRRIALTD AGQRYLDRCQ RILAYIDEAE AEAADAQAKP SGRLHVHATT SFGQAYVVPA VVRYRQRYPS VAVELTLSQH VPDIIDEGYD VSLQLSTAEL PDSGLVSQRL GEMHSVLCAS PAYLKERGTP RTVSDLEGHA CLQIVTPIFP RDRWHLDGPN GRETFELPLP DFQVNIADAL GAGLRAGLGI GALPMSTALP AFASGALVRV LPEYRLQKLT VYTLYASRQY LDAKIRTFVD FLRECVPEML AADEAALCAA QDARANA

NT Sequence

ATGGACACGT TACAGATGAT GCGCATTTTT GTCCGGGTCG CGGAGGAGGG CAGCTTTACG AGCGCCGCCC AGCGGCTGGA CATTACGACA GCCTACGCAT CGCGCTCGGT CGCGCAGCTG GAAACGCATC TGCGCACGCG TCTGCTGAAT CGCAGCACCC GCCGCATTGC GCTGACGGAC GCAGGCCAGC GCTACCTCGA CCGTTGCCAG CGGATTCTCG CGTATATCGA CGAAGCCGAA GCCGAGGCGG CCGACGCGCA GGCAAAGCCG TCGGGGCGAT TGCACGTGCA CGCGACGACG AGTTTCGGGC AGGCGTACGT CGTGCCTGCG GTCGTTCGCT ATCGACAGCG TTATCCGTCG GTCGCGGTGG AACTGACGCT GTCGCAGCAC GTGCCCGACA TCATCGACGA AGGCTACGAC GTGTCGCTGC AGCTGAGCAC GGCCGAACTG CCCGACTCCG GCCTCGTTTC GCAGCGGCTC GGCGAAATGC ATAGCGTGCT GTGCGCGTCG CCTGCCTATC TGAAAGAGCG CGGCACGCCG CGTACCGTGA GCGATCTGGA AGGGCACGCC TGCCTGCAGA TCGTCACGCC GATCTTTCCG CGCGATCGCT GGCATCTCGA CGGGCCCAAC GGCCGCGAGA CGTTCGAACT GCCGTTGCCC GACTTTCAGG TGAACATCGC CGACGCGCTG GGCGCGGGGC TGCGCGCCGG GCTCGGCATC GGCGCATTGC CGATGTCGAC GGCCTTGCCG GCATTCGCGA GCGGCGCGCT CGTGCGCGTG TTGCCCGAAT ACCGGCTGCA GAAGCTGACC GTCTATACGC TCTATGCCTC GCGTCAGTAT CTCGATGCGA AGATCAGGAC GTTCGTCGAT TTTCTGCGCG AGTGCGTGCC GGAAATGCTC GCGGCCGACG AGGCCGCACT CTGTGCGGCG CAGGATGCGC GCGCGAACGC G

Details for BumuA.12800.y.A1.GE34673

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLQMMRI FVRVAEEGSF TSAAQRLDIT TAYASRSVAQ LETHLRTRLL NRSTRRIALT DAGQRYLDRC QRILAYIDEA EAEAADAQAK PSGRLHVHAT TSFGQAYVVP AVVRYRQRYP SVAVELTLSQ HVPDIIDEGY DVSLQLSTAE LPDSGLVSQR LGEMHSVLCA SPAYLKERGT PRTVSDLEGH ACLQIVTPIF PRDRWHLDGP NGRETFELPL PDFQVNIADA LGAGLRAGLG IGALPMSTAL PAFASGALVR VLPEYRLQKL TVYTLYASRQ YLDAKIRTFV DFLRECVPEM LAADEAALCA AQDARA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaca cgttacagat gatgcgcatt tttgtccggg tcgcggagga gggcagcttt acgagcgccg cccagcggct ggacattacg acagcctacg catcgcgctc ggtcgcgcag ctggaaacgc atctgcgcac gcgtctgctg aatcgcagca cccgccgcat tgcgctgacg gacgcaggcc agcgctacct cgaccgttgc cagcggattc tcgcgtatat cgacgaagcc gaagccgagg cggccgacgc gcaggcaaag ccgtcggggc gattgcacgt gcacgcgacg acgagtttcg ggcaggcgta cgtcgtgcct gcggtcgttc gctatcgaca gcgttatccg tcggtcgcgg tggaactgac gctgtcgcag cacgtgcccg acatcatcga cgaaggctac gacgtgtcgc tgcagctgag cacggccgaa ctgcccgact ccggcctcgt ttcgcagcgg ctcggcgaaa tgcatagcgt gctgtgcgcg tcgcctgcct atctgaaaga gcgcggcacg ccgcgtaccg tgagcgatct ggaagggcac gcctgcctgc agatcgtcac gccgatcttt ccgcgcgatc gctggcatct cgacgggccc aacggccgcg agacgttcga actgccgttg cccgactttc aggtgaacat cgccgacgcg ctgggcgcgg ggctgcgcgc cgggctcggc atcggcgcat tgccgatgtc gacggccttg ccggcattcg cgagcggcgc gctcgtgcgc gtgttgcccg aataccggct gcagaagctg accgtctata cgctctatgc ctcgcgtcag tatctcgatg cgaagatcag gacgttcgtc gattttctgc gcgagtgcgt gccggaaatg ctcgcggccg acgaggccgc actctgtgcg gcgcaggatg cgcgcgcga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacacgtta cagatgatgc gcatttttgt ccgggtcgcg gaggagggca gctttacgag cgccgcccag cggctggaca ttacgacagc ctacgcatcg cgctcggtcg cgcagctgga aacgcatctg cgcacgcgtc tgctgaatcg cagcacccgc cgcattgcgc tgacggacgc aggccagcgc tacctcgacc gttgccagcg gattctcgcg tatatcgacg aagccgaagc cgaggcggcc gacgcgcagg caaagccgtc ggggcgattg cacgtgcacg cgacgacgag tttcgggcag gcgtacgtcg tgcctgcggt cgttcgctat cgacagcgtt atccgtcggt cgcggtggaa ctgacgctgt cgcagcacgt gcccgacatc atcgacgaag gctacgacgt gtcgctgcag ctgagcacgg ccgaactgcc cgactccggc ctcgtttcgc agcggctcgg cgaaatgcat agcgtgctgt gcgcgtcgcc tgcctatctg aaagagcgcg gcacgccgcg taccgtgagc gatctggaag ggcacgcctg cctgcagatc gtcacgccga tctttccgcg cgatcgctgg catctcgacg ggcccaacgg ccgcgagacg ttcgaactgc cgttgcccga ctttcaggtg aacatcgccg acgcgctggg cgcggggctg cgcgccgggc tcggcatcgg cgcattgccg atgtcgacgg ccttgccggc attcgcgagc ggcgcgctcg tgcgcgtgtt gcccgaatac cggctgcaga agctgaccgt ctatacgctc tatgcctcgc gtcagtatct cgatgcgaag atcaggacgt tcgtcgattt tctgcgcgag tgcgtgccgg aaatgctcgc ggccgacgag gccgcactct gtgcggcgca ggatgcgcgc gcgaacgcgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa