SSGCID: Target Page

BumuA.12800.x

LysR family transcriptional regulator

CENTER ID:	BumuA.12800.x
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.x.A1.GE34668	Full length( BumuA.12800.x )		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.6379
RefSeq:	YP_001586005.1
UniProt:	A0A0H3KPY8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MELNDLRIFV ATVDAGSFTA AADQLMLSKQ FVSRRTMALE ASLGVRLLHR NTRNLAVTES GQEFYARAQR ILAEVADAEQ AMSVRSTELH GSLKISAPLS FGITHVSPLI AEFLSAHPAV RLNLDLTDRR VDVIGEGFDL VLRIGALEDS TLIARALGAW KMIACASPAY LKRHGTPATP AELANHTCLL YGRERRVGWE FRVDGAPRTF DVQGPLVANN GEVVRDAAIA GLGIALLPSF IVGAALDGGA LVPVLDAYAP APITLNAVFP QHREGFVTLR TFIGFLAERL GPRA

NT Sequence

ATGGAACTCA ACGACCTCCG GATTTTTGTC GCGACCGTCG ATGCGGGCAG TTTCACCGCG GCGGCCGATC AACTGATGCT GTCGAAGCAG TTCGTCAGCC GGCGCACGAT GGCGCTCGAG GCGTCGCTCG GCGTGCGCCT GCTGCACCGG AACACGCGCA ATCTCGCCGT GACCGAATCG GGCCAGGAGT TCTATGCGCG CGCGCAGCGG ATCCTCGCGG AAGTCGCCGA TGCCGAGCAG GCGATGTCGG TGCGCAGCAC CGAACTGCAC GGTTCGCTGA AGATCAGCGC GCCGCTGTCG TTCGGGATCA CGCACGTCTC GCCGCTGATC GCCGAGTTCC TGTCCGCGCA TCCGGCCGTG CGGCTCAATC TCGATCTGAC CGATCGGCGC GTCGACGTGA TCGGCGAGGG CTTCGATCTC GTGCTGCGGA TCGGCGCGCT CGAGGATTCG ACGCTGATCG CGCGGGCGCT CGGCGCATGG AAGATGATCG CGTGCGCGAG CCCCGCCTAT CTGAAGCGGC ACGGCACGCC GGCGACGCCG GCCGAGCTCG CGAATCACAC GTGCCTGCTG TACGGGCGCG AGCGGCGCGT CGGCTGGGAG TTCCGCGTCG ACGGCGCGCC GCGCACGTTC GACGTGCAGG GGCCGCTCGT CGCGAACAAC GGCGAAGTCG TGCGCGATGC GGCAATCGCG GGGCTCGGCA TCGCGTTGCT GCCGTCCTTC ATCGTCGGCG CGGCGCTCGA CGGCGGCGCG CTCGTGCCGG TGCTCGACGC GTATGCGCCC GCGCCGATCA CGCTGAACGC GGTGTTTCCG CAGCATCGCG AAGGCTTCGT CACGCTGCGC ACCTTCATCG GCTTTCTCGC CGAACGCCTC GGTCCGCGCG CG

Details for BumuA.12800.x.A1.GE34668

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMELNDLR IFVATVDAGS FTAAADQLML SKQFVSRRTM ALEASLGVRL LHRNTRNLAV TESGQEFYAR AQRILAEVAD AEQAMSVRST ELHGSLKISA PLSFGITHVS PLIAEFLSAH PAVRLNLDLT DRRVDVIGEG FDLVLRIGAL EDSTLIARAL GAWKMIACAS PAYLKRHGTP ATPAELANHT CLLYGRERRV GWEFRVDGAP RTFDVQGPLV ANNGEVVRDA AIAGLGIALL PSFIVGAALD GGALVPVLDA YAPAPITLNA VFPQHREGFV TLRTFIGFLA ERLGPRA

Validated NT Sequence

ttattacgcg cgcggaccga ggcgttcggc gagaaagccg atgaaggtgc gcagcgtgac gaagccttcg cgatgctgcg gaaacaccgc gttcagcgtg atcggcgcgg gcgcatacgc gtcgagcacc ggcacgagcg cgccgccgtc gagcgccgcg ccgacgatga aggacggcag caacgcgatg ccgagccccg cgattgccgc atcgcgcacg acttcgccgt tgttcgcgac gagcggcccc tgcacgtcga acgtgcgcgg cgcgccgtcg acgcggaact cccagccgac gcgccgctcg cgcccgtaca gcaggcacgt gtgattcgcg agctcggccg gcgtcgccgg cgtgccgtgc cgcttcagat aggcggggct cgcgcacgcg atcatcttcc atgcgccgag cgcccgcgcg atcagcgtcg aatcctcgag cgcgccgatc cgcagcacga gatcgaagcc ctcgccgatc acgtcgacgc gccgatcggt cagatcgaga ttgagccgca cggccggatg cgcggacagg aactcggcga tcagcggcga gacgtgcgtg atcccgaacg acagcggcgc gctgatcttc agcgaaccgt gcagttcggt gctgcgcacc gacatcgcct gctcggcatc ggcgacttcc gcgaggatcc gctgcgcgcg cgcatagaac tcctggcccg attcggtcac ggcgagattg cgcgtgttcc ggtgcagcag gcgcacgccg agcgacgcct cgagcgccat cgtgcgccgg ctgacgaact gcttcgacag catcagttga tcggccgccg cggtgaaact gcccgcatcg acggtcgcga caaaaatccg gaggtcgttg agttccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMELNDLRIF VATVDAGSFT AAADQLMLSK QFVSRRTMAL EASLGVRLLH RNTRNLAVTE SGQEFYARAQ RILAEVADAE QAMSVRSTEL HGSLKISAPL SFGITHVSPL IAEFLSAHPA VRLNLDLTDR RVDVIGEGFD LVLRIGALED STLIARALGA WKMIACASPA YLKRHGTPAT PAELANHTCL LYGRERRVGW EFRVDGAPRT FDVQGPLVAN NGEVVRDAAI AGLGIALLPS FIVGAALDGG ALVPVLDAYA PAPITLNAVF PQHREGFVTL RTFIGFLAER LGPRA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaactcaac gacctccgga tttttgtcgc gaccgtcgat gcgggcagtt tcaccgcggc ggccgatcaa ctgatgctgt cgaagcagtt cgtcagccgg cgcacgatgg cgctcgaggc gtcgctcggc gtgcgcctgc tgcaccggaa cacgcgcaat ctcgccgtga ccgaatcggg ccaggagttc tatgcgcgcg cgcagcggat cctcgcggaa gtcgccgatg ccgagcaggc gatgtcggtg cgcagcaccg aactgcacgg ttcgctgaag atcagcgcgc cgctgtcgtt cgggatcacg cacgtctcgc cgctgatcgc cgagttcctg tccgcgcatc cggccgtgcg gctcaatctc gatctgaccg atcggcgcgt cgacgtgatc ggcgagggct tcgatctcgt gctgcggatc ggcgcgctcg aggattcgac gctgatcgcg cgggcgctcg gcgcatggaa gatgatcgcg tgcgcgagcc ccgcctatct gaagcggcac ggcacgccgg cgacgccggc cgagctcgcg aatcacacgt gcctgctgta cgggcgcgag cggcgcgtcg gctgggagtt ccgcgtcgac ggcgcgccgc gcacgttcga cgtgcagggg ccgctcgtcg cgaacaacgg cgaagtcgtg cgcgatgcgg caatcgcggg gctcggcatc gcgttgctgc cgtccttcat cgtcggcgcg gcgctcgacg gcggcgcgct cgtgccggtg ctcgacgcgt atgcgcccgc gccgatcacg ctgaacgcgg tgtttccgca gcatcgcgaa ggcttcgtca cgctgcgcac cttcatcggc tttctcgccg aacgcctcgg tccgcgcgcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa