SSGCID: Target Page

BumuA.12800.w

LysR family transcriptional regulator

CENTER ID:	BumuA.12800.w
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.12800.w.A1.GE34676	Full length( BumuA.12800.w )		1	323

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.4417
RefSeq:	YP_001584093.1
UniProt:	A0A0H3KLW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDMLENMRLF VRVVEAGSFT AVAKEIDATT AQVSRAVSNL EAHVQTRLLH RTTRHLGLTE SGERYFERAK SILAEIDYAN AEARNALLRP SGKMRIHAMT GLGQSHVVSS IVRYQEDNPE VSVELTLAQR MPNLVEEGYD VSIVTASQLP DSGYVAQTCG TSCSVLVASR DYLARHGTPQ TPDDLPNHVC LRLDTPASPA SEWRLERADG EETVYELQPA PFQVNVPDAL CVAVRAGRGI ACVALYTVLD DIREGRLIRV LPEYRLQTVS VYAVYATRRY LDAKIRTFLD HLRTTLTPAL ENDLRELDRL TIEQAQSGGL KRA

NT Sequence

ATGGACATGC TCGAAAACAT GCGCCTGTTC GTGCGCGTTG TCGAAGCCGG CAGTTTCACC GCGGTTGCGA AAGAGATCGA TGCGACCACG GCGCAGGTGT CGCGCGCCGT GTCGAATCTC GAGGCGCACG TGCAGACGCG TCTTCTGCAT CGCACGACGC GCCACCTCGG TCTGACCGAA AGCGGCGAGC GCTATTTCGA GCGCGCGAAA TCGATCCTTG CGGAGATCGA CTACGCGAAC GCCGAGGCGC GCAATGCGCT GCTGCGGCCG AGCGGGAAGA TGCGCATCCA TGCGATGACG GGGCTCGGGC AGAGTCATGT CGTGTCGTCG ATCGTCCGCT ATCAGGAGGA CAACCCCGAG GTGTCGGTCG AGCTGACGCT TGCGCAGCGC ATGCCGAACC TCGTCGAGGA AGGGTACGAC GTGTCGATCG TCACGGCGTC GCAGTTGCCC GATTCGGGCT ACGTCGCGCA GACCTGCGGC ACGAGCTGCA GCGTGCTCGT CGCGTCGCGC GACTATCTCG CGCGGCACGG TACGCCGCAG ACGCCGGACG ATCTGCCGAA CCACGTGTGC CTGCGCCTCG ACACACCGGC GTCGCCCGCG AGCGAGTGGC GGCTCGAACG CGCCGACGGC GAGGAAACCG TGTACGAACT GCAGCCCGCG CCGTTCCAGG TCAACGTGCC GGACGCGCTC TGCGTCGCAG TGCGCGCCGG GCGCGGGATC GCCTGCGTCG CGCTCTATAC GGTGCTCGAC GACATCCGCG AAGGGCGGCT GATCCGCGTG CTGCCCGAGT ACCGGTTGCA GACCGTCAGC GTCTATGCGG TCTATGCGAC GCGCCGCTAT CTCGACGCGA AGATCCGCAC GTTCCTCGAC CATCTGCGCA CGACGCTGAC GCCGGCGCTG GAGAACGATC TGCGCGAGCT CGACCGGCTC ACGATCGAGC AGGCGCAAAG CGGCGGTCTG AAGCGCGCG

Details for BumuA.12800.w.A1.GE34676

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDMLENMRL FVRVVEAGSF TAVAKEIDAT TAQVSRAVSN LEAHVQTRLL HRTTRHLGLT ESGERYFERA KSILAEIDYA NAEARNALLR PSGKMRIHAM TGLGQSHVVS SIVRYQEDNP EVSVELTLAQ RMPNLVEEGY DVSIVTASQL PDSGYVAQTC GTSCSVLVAS RDYLARHGTP QTPDDLPNHV CLRLDTPASP ASEWRLERAD GEETVYELQP APFQVNVPDA LCVAVRAGRG IACVALYTVL DDIREGRLIR VLPEYRLQTV SVYAVYATRR YLDAKIRTFL DHLRTTLTPA LENDLRELDR LTIEQAQSGG LKRA

Validated NT Sequence

ttaagaagga gntataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggacatg ctcgaaaaca tgcgcctgtt cgtgcgcgtt gtcgaagccg gcagtttcac cgcggttgcg aaagagatcg atgcgaccac ggcgcaggtg tcgcgcgccg tgtcgaatct cgaggcgcac gtgcagacgc gtcttctgca tcgcacgacg cgccacctcg gtctgaccga aagcggcgag cgctatttcg agcgcgcgaa atcgatcctt gcggagatcg actacgcgaa cgccgaggcg cgcaatgcgc tgctgcggcc gagcgggaag atgcgcatcc atgcgatgac ggggctcggg cagagtcatg tcgtgtcgtc gatcgtccgc tatcaggagg acaaccccga ggtgtcggtc gagctgacgc ttgcgcagcg catgccgaac ctcgtcgagg aagggtacga cgtgtcgatc gtcacggcgt cgcagttgcc cgattcgggc tacgtcgcgc agacctgcgg cacgagctgc agcgtgctcg tcgcgtcgcg cgactatctc gcgcggcacg gtacgccgca gacgccggac gatctgccga accacgtgtg cctgcgcctc gacacaccgg cgtcgcccgc gagcgagtgg cggctcgaac gcgccgacgg cgaggaaacc gtgtacgaac tgcagcccgc gccgttccag gtcaacgtgc cggacgcgct ctgcgtcgca gtgcgcgccg ggcgcgggat cgcctgcgtc gcgctctata cggtgctcga cgacatccgc gaagggcggc tgatccgcgt gctgcccgag taccggttgc agaccgtcag cgtctatgcg gtctatgcga cgcgccgcta tctcgacgcg aagatccgca cgttcctcga ccatctgcgc acgacgctga cgccggcgct ggagaacgat ctgcgcgagc tcgaccggct cacgatcgag caggcgcaaa gcggcggtct gaagcgcgcg taata

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacatgctc gaaaacatgc gcctgttcgt gcgcgttgtc gaagccggca gtttcaccgc ggttgcgaaa gagatcgatg cgaccacggc gcaggtgtcg cgcgccgtgt cgaatctcga ggcgcacgtg cagacgcgtc ttctgcatcg cacgacgcgc cacctcggtc tgaccgaaag cggcgagcgc tatttcgagc gcgcgaaatc gatccttgcg gagatcgact acgcgaacgc cgaggcgcgc aatgcgctgc tgcggccgag cgggaagatg cgcatccatg cgatgacggg gctcgggcag agtcatgtcg tgtcgtcgat cgtccgctat caggaggaca accccgaggt gtcggtcgag ctgacgcttg cgcagcgcat gccgaacctc gtcgaggaag ggtacgacgt gtcgatcgtc acggcgtcgc agttgcccga ttcgggctac gtcgcgcaga cctgcggcac gagctgcagc gtgctcgtcg cgtcgcgcga ctatctcgcg cggcacggta cgccgcagac gccggacgat ctgccgaacc acgtgtgcct gcgcctcgac acaccggcgt cgcccgcgag cgagtggcgg ctcgaacgcg ccgacggcga ggaaaccgtg tacgaactgc agcccgcgcc gttccaggtc aacgtgccgg acgcgctctg cgtcgcagtg cgcgccgggc gcgggatcgc ctgcgtcgcg ctctatacgg tgctcgacga catccgcgaa gggcggctga tccgcgtgct gcccgagtac cggttgcaga ccgtcagcgt ctatgcggtc tatgcgacgc gccgctatct cgacgcgaag atccgcacgt tcctcgacca tctgcgcacg acgctgacgc cggcgctgga gaacgatctg cgcgagctcg accggctcac gatcgagcag gcgcaaagcg gcggtctgaa gcgcgcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa